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61. 发明授权

US4604351A Method for determining bacterial sensitivity to chemical agents 失效
标题翻译：确定细菌对化学药剂的敏感性的方法
公开(公告)号：US4604351A
公开(公告)日：1986-08-05
申请号：US630736
申请日：1984-07-13
申请人： Leonard Amaral
发明人： Leonard Amaral
IPC分类号： C12M1/34 , C12Q1/18 , C12Q1/16 , C12Q1/02
CPC分类号： C12Q1/18
摘要： The effect of a chemical on bacterial growth is rapidly determined by culturing a sample of bacteria in the presence of said chemical for a period of time sufficient to obtain detectable bacterial growth. During the culturing period, a labelled nucleotide is added to the growth media and the uptake of said nucleotide is measured and compared to a control.
摘要翻译：化学品对细菌生长的影响通过在所述化学品存在下培养细菌样品足以获得可检测的细菌生长的时间来快速测定。在培养期间，将标记的核苷酸加入到生长培养基中，并测量所述核苷酸的摄取并与对照进行比较。

62. 发明授权

US4563419A Detection of microbial nucleic acids by a one-step sandwich hybridization test 失效
标题翻译：通过一步夹心杂交试验检测微生物核酸
公开(公告)号：US4563419A
公开(公告)日：1986-01-07
申请号：US566532
申请日：1983-12-29
申请人： Tuula M. Ranki , Hans E. Soderlund
发明人： Tuula M. Ranki , Hans E. Soderlund
IPC分类号： G01N33/50 , C12N15/00 , C12N15/09 , C12Q1/04 , C12Q1/16 , C12Q1/68 , C12Q1/6813 , C12Q1/6888 , C12Q1/689 , C12Q1/70 , C12R1/07 , C12R1/91 , G01N33/58
CPC分类号： C12Q1/04 , C12Q1/6813 , C12Q1/6888 , C12Q1/689 , C12Q1/701 , Y10S435/81 , Y10S436/804 , Y10S436/808 , Y10S436/811 , Y10S436/823
摘要： This invention relates to a kit for the detection of microbial nucleic acids and a method for identifying the nucleic acids using a one-step sandwich hybridization technique. The technique requires two complementary nucleic acid reagents for each microbe or group of microbes to be identified.

63. 发明授权

US4486539A Detection of microbial nucleic acids by a one-step sandwich hybridization test 失效
标题翻译：通过一步夹心杂交试验检测微生物核酸
公开(公告)号：US4486539A
公开(公告)日：1984-12-04
申请号：US434182
申请日：1982-10-14
申请人： Tuula M. Ranki , Soderlund Hans E.
发明人： Tuula M. Ranki , Soderlund Hans E.
IPC分类号： G01N33/50 , C12N15/00 , C12N15/09 , C12Q1/04 , C12Q1/16 , C12Q1/68 , C12Q1/6813 , C12Q1/6888 , C12Q1/689 , C12Q1/70 , C12R1/07 , C12R1/91 , G01N33/58
CPC分类号： C12Q1/04 , C12Q1/6813 , C12Q1/6888 , C12Q1/689 , C12Q1/701 , Y10S435/81 , Y10S436/804 , Y10S436/808 , Y10S436/811 , Y10S436/823
摘要： This invention relates to a diagnostic kit based on a one step hybridization procedure and method of using the kit for identifying the nucleic acids of viruses and bacteria contained in a single sample. The procedure requires two nucleic acid reagents for each microbe or group of microbes to be identified.
摘要翻译：本发明涉及一种基于一步杂交方法的诊断试剂盒和使用该试剂盒鉴定单个样品中所含的病毒和细菌核酸的方法。该方法需要鉴定每种微生物或一组微生物的两种核酸试剂。

64. 发明授权

US4446237A Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid 失效
标题翻译：在无细胞生物液体中检测可疑病毒脱氧核糖核酸的方法
公开(公告)号：US4446237A
公开(公告)日：1984-05-01
申请号：US248048
申请日：1981-03-27
申请人： Mark S. Berninger
发明人： Mark S. Berninger
IPC分类号： G01N33/50 , C12Q1/16 , C12Q1/68 , C12Q1/6813 , C12Q1/70 , G01N33/60 , G01N33/58 , C12N15/00
CPC分类号： G01N33/60 , C12Q1/6813 , C12Q1/70
摘要： There is disclosed a method for evaluating acellular biological fluid for viral DNA, such as DNA of the Hepatitus-B virus, whereby the acellular biological fluid is treated to immobilize in denatured form the DNAs including the suspect viral DNA on a solid support or substrate with the resulting solid substrate being contacted with a solution including radioisotropically-labeled viral denatured DNA followed by analysis of solid substrate for radioisotopically-labeled suspect viral renatured DNA.
摘要翻译：公开了一种用于评估病毒DNA的无细胞生物流体的方法，例如Hepatitus-B病毒的DNA，由此无细胞生物流体被处理以在固体支持物或底物上变性形式包含可疑病毒DNA的DNA，使得到的固体底物与包括放射性标记的病毒变性DNA的溶液接触，随后分析用于放射性同位素标记的可疑病毒复性DNA的固体底物。

65. 发明授权

US4416995A Method and apparatus for determining bacterial sensitivity 失效
标题翻译：用于确定细菌敏感性的方法和装置
公开(公告)号：US4416995A
公开(公告)日：1983-11-22
申请号：US300943
申请日：1981-09-10
申请人： Leonard Amaral
发明人： Leonard Amaral
IPC分类号： C12M1/34 , C12Q1/18 , C12M1/12 , C12Q1/16
CPC分类号： C12Q1/18 , Y10S435/803
摘要： Method, apparatus and automated system for determining the effects of a chemical agent upon a bacterial sample. Aliquots of a bacterial suspension in a culture medium, the chemical agent, and tritiated thymidine are deposited in polyethylene tubes having first and second compartments separated by a fixed millipore filter. A precipitation agent, such as trichloroacetic acid, is deposited into the polyethylene tubes to precipitate the bacteria. The bacteria is separated from the liquid by operation of a vacuum system which draws the liquid through the filter, thereby filtering out the bacteria. The filtered bacteria is subsequently washed with a washing solution. In one embodiment, a scintillation fluid is added to the bacteria, the bacteria then being conveyed to a scintillation counter which produces an output signal corresponding to the amount of radiation emanating from the bacteria. The amount of emanating radiation corresponds to the amount of tritiated thymidine which is incorporated into the bacteria, and hence is a measure of the rate of synthesis of DNA. In this manner, bacterial activity in the presence of a chemical agent is determined prior to a change in the bacterial population.
摘要翻译：用于确定化学试剂对细菌样品的影响的方法，装置和自动化系统。在培养基，化学试剂和氚标记胸苷中的细菌悬浮液的等分试样沉积在具有由固定的微孔过滤器分离的第一和第二隔室的聚乙烯管中。沉淀剂如三氯乙酸沉积在聚乙烯管中以使细菌沉淀。通过操作真空系统将细菌从液体中分离出来，该真空系统将液体通过过滤器，从而过滤细菌。随后用洗涤溶液洗涤过滤的细菌。在一个实施方案中，向细菌中加入闪烁液，然后将细菌输送到闪烁计数器，其产生对应于从细菌发出的辐射量的输出信号。发射辐射的量对应于掺入细菌中的氚标记的胸苷的量，因此是DNA合成速率的量度。以这种方式，在细菌群体的变化之前测定在化学试剂存在下的细菌活性。

66. 发明授权

US3997404A Method and apparatus for characterizing biologically active agents 失效
标题翻译：用于表征生物活性剂的方法和装置
公开(公告)号：US3997404A
公开(公告)日：1976-12-14
申请号：US581277
申请日：1975-05-27
申请人： John R. Waters
发明人： John R. Waters
IPC分类号： C12M1/34 , C12Q1/16 , G01N21/76 , C12K1/04
CPC分类号： C12Q1/16 , C12M41/34 , C12M41/36 , G01N21/76 , Y10S435/808
摘要： A method for characterization of biological activity comprising providing a plurality of gas-tight cells each having a light-permeable wall section, each cell containing a different .sup.14 C-labeled fermentable substrate, a carbon dioxide absorber and a scintillation compound in intimate proximity to each other remote from said substrate, and an axenic sample of a biologically active material; subjecting said cells to conditions conducive to biological activity and thereafter measuring the light emitted by the scintillation compound as a result of the decay of .sup.14 CO.sub.2 generated by the metabolic action of the biologically active agent in said sample on said substrate and captured by the carbon dioxide absorber. Apparatus is provided comprising a plurality of sterile chambers containing different .sup.14 C-labeled fermentable substrates, means to facilitate introduction of an axenic sample of a biologically active agent into each chamber, means for sealing each chamber to form a gas-tight cell having a light-permeable wall section, and a carbon dioxide absorber and a scintillation compound in intimate proximity to each other in each cell adjacent the light-permeable wall section.
摘要翻译：一种用于表征生物活性的方法，包括提供多个具有透光壁部分的气密细胞，每个细胞含有不同的14C标记的可发酵底物，二氧化碳吸收剂和闪烁化合物彼此紧邻远离所述衬底，以及生物活性材料的无色样品; 对所述细胞进行有利于生物活性的条件，然后测量由闪烁化合物发射的光，其是由所述样品中所述生物活性剂在所述底物上的代谢作用产生的14CO 2的衰变并由二氧化碳吸收剂。提供了包括多个含有不同14C标记的可发酵底物的无菌室的装置，有助于将生物活性剂的无菌样品引入每个室中的装置，用于密封每个室以形成具有发光基底的气密细胞的装置，可渗透壁部分，以及邻近透光壁部分的每个细胞中彼此紧密相邻的二氧化碳吸收剂和闪烁体。

67. 发明授权

US3844894A Apparatus for performing assays on reactions that produce radioactive gases 失效
标题翻译：用于对产生放射性气体的反应进行测定的装置
公开(公告)号：US3844894A
公开(公告)日：1974-10-29
申请号：US17590371
申请日：1971-08-30
申请人： US HEALTH EDUCATION & WELFARE
发明人： KRONICK M , WILSON S
IPC分类号： C12Q1/00 , C12Q1/16 , G01T1/20 , G01T7/02 , G01N31/14
CPC分类号： G01T1/20 , C12Q1/00 , C12Q1/16 , G01T7/02 , Y10S435/808
摘要： A method for performing assays on reactions that produce radioactive gases. Reaction components are placed in the reaction well of an open bottomed container. The reaction is sealed except for an aperture through which evolved radioactive gases can escape. A tent-like cover of scintillation plastic surrounds the aperture. When the radioactive gases strike the plastic, scintillations will be emitted and may be counted by a standard scintillation counter. This method is particularly useful in performing enzyme assays.
摘要翻译：对产生放射性气体的反应进行测定的方法。将反应组分置于开口的容器的反应槽中。除了放出的放射性气体可以逸出的孔之外，反应是密封的。闪烁塑料的帐篷式盖子围绕孔。当放射性气体撞击塑料时，会发出闪烁，并可用标准闪烁计数器计数。该方法在进行酶测定中特别有用。

68. 发明授权

US11933790B2 Protein composition and methods for analysing microbiota 有权
公开(公告)号：US11933790B2
公开(公告)日：2024-03-19
申请号：US17159731
申请日：2021-01-27
申请人： UNIVERSITY OF OTTAWA
发明人： Joseph Michel Daniel Figeys , Alain Christophe Stintzi , David R. Mack , Xu Zhang , Zhibin Ning
IPC分类号： G01N33/68 , A61B5/00 , C12Q1/16 , G01N33/50 , G16B30/10 , G16B40/10 , G16B40/20 , G16H50/20 , G16H50/30 , G16H50/70
CPC分类号： G01N33/6848 , A61B5/4255 , C12Q1/16 , G01N33/5023 , G01N33/5091 , G16B30/10 , G16B40/10 , G16B40/20 , G16H50/20 , G16H50/30 , G16H50/70 , A61B2503/42 , G01N2223/01 , G01N2570/00 , G01N2800/06
摘要： A method of isotope-labelling a microbiota sample. It involves providing a first microbiota sample that was obtained from a given source; exposing the first microbiota sample to an isotope enriched medium; and culturing the exposed first microbiota sample in the isotope enriched medium to obtain an isotope-labelled microbiota sample, wherein the isotope labelled metaproteome of the isotope-labelled microbiota sample is taxon specific for taxa present in the first microbiota sample when initially obtained from the given source.

69. 发明公开

US20230193346A1 METHOD FOR DETECTING MYCOBACTERIUM TUBERCULOSIS 审中-公开
公开(公告)号：US20230193346A1
公开(公告)日：2023-06-22
申请号：US17923403
申请日：2021-04-29
申请人： KOREA ATOMIC ENERGY RESEARCH INSTITUTE , CHUNGNAM NATIONAL UNIVERSITY HOSPITAL
发明人： Jae Cheong LIM , Eun Ha CHO , So Young LEE , Sung Hee JUNG , Chae Uk CHUNG
IPC分类号： C12Q1/16 , C12Q1/58
CPC分类号： C12Q1/16 , C12Q1/58 , C12R2001/32
摘要： The present invention relates to a method for detecting Mycobacterium tuberculosis, which can replace the conventional culture method that takes a long time of four to eight weeks to detect active tuberculosis, and which is a method for detecting active Mycobacterium tuberculosis by using isotopes on a sample of a patient's sputum or bronchoalveolar lavage fluid.

70. 发明申请

US20230133705A1 TREHALOSE ANALOGUES 有权
公开(公告)号：US20230133705A1
公开(公告)日：2023-05-04
申请号：US18068852
申请日：2022-12-20
申请人： Central Michigan University
发明人： Benjamin M. Swarts
IPC分类号： C07H7/06 , G01N33/60 , C12Q1/16 , G01N33/58 , C12Q1/04
摘要： Described herein are trehalose analogues. Also described herein are methods of making the trehalose analogues and uses of the analogues. For example, the disclosed trehalose analogues may be useful in the detection of bacteria.

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