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61. 发明授权

US06303339B1 DNA segments encoding autoimmune polypeptide epitopes of Ro/ss-A antigen 失效
标题翻译：编码Ro /β-A抗原自身免疫多肽表位的DNA段
公开(公告)号：US06303339B1
公开(公告)日：2001-10-16
申请号：US07576423
申请日：1991-05-24
申请人： Richard D. Sontheimer , Tsu-San Lieu , Daniel P. McCauliffe , J. Donald Capra
发明人： Richard D. Sontheimer , Tsu-San Lieu , Daniel P. McCauliffe , J. Donald Capra
IPC分类号： C12P2104
CPC分类号： G01N33/564 , C07K14/46 , C12Q1/6876 , G01N2800/24
摘要： The present disclosure relates to DNA sequences encoding one or more antigenic epitopes of the Ro 60 kD autoantigen, as well as to antigenic peptides themselves which correspond antigenically to epitopes found on the Ro/SS-A ribonucleoprotein (RNP) particle. Peptides which incorporate the antigenic epitopic core sequences disclosed herein may be employed in place of the Ro/SS-A RNP in any of a variety immunoassays including ELISA assays. The polypeptides of the invention may be employed in colorimetric assays for the identification and characterization of autoimmune diseases such as systemic lupus erythematosus (SLE) and Sjogren's syndrome. The DNA sequences disclosed herein may be employed in the preparation of the 60 kD Ro antigen, peptides which incorporate antigenic core sequences thereof, to probe for Ro sequences by hybridization analysis, and the like.
摘要翻译：本公开内容涉及编码Ro 60kD自身抗原的一种或多种抗原表位的DNA序列，以及抗原肽本身对应于在Ro / SS-A核糖核蛋白（RNP）颗粒上发现的表位的抗原性肽。可以使用包含本文公开的抗原性表位核心序列的肽来代替Ro / SS-A RNP，其包括ELISA测定中的任一种免疫测定。本发明的多肽可用于鉴定和表征自身免疫疾病如系统性红斑狼疮（SLE）和干燥综合征的比色测定。本文公开的DNA序列可以用于制备60kD Ro抗原，其中包含其抗原核心序列的肽，通过杂交分析来探测Ro序列等。

62. 发明授权

US06287805B1 Nucleic acid molecules of the protein-coupled heptahelical receptor superfamily and uses therefor 失效
标题翻译：蛋白偶联的七面体受体超家族的核酸分子和用途
公开(公告)号：US06287805B1
公开(公告)日：2001-09-11
申请号：US09045583
申请日：1998-03-20
申请人： Gerard J. Graham , Robert J. Benjamin Nibbs , Jose-Angel Gonzalo , Jose-Carlos Gutierrez-Ramos
发明人： Gerard J. Graham , Robert J. Benjamin Nibbs , Jose-Angel Gonzalo , Jose-Carlos Gutierrez-Ramos
IPC分类号： C12P2104
CPC分类号： C07K14/7158 , A61K38/00
摘要： Novel D6 polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length D6 proteins, the invention further provides isolated D6 fusion proteins, antigenic peptides and anti-D6 antibodies. The invention also provides D6 nucleic acid molecules, recombinant expression vectors containing nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a D6 gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.
摘要翻译：公开了新型D6多肽，蛋白质和核酸分子。除了分离的全长D6蛋白之外，本发明还提供分离的D6融合蛋白，抗原肽和抗D6抗体。本发明还提供了D6核酸分子，含有本发明的核酸分子的重组表达载体，已经引入了表达载体的宿主细胞和其中D6基因被引入或被破坏的非人转基因动物。还提供利用本发明组合物的诊断，筛选和治疗方法。

63. 发明授权

US06258565B1 Expression vectors based on RK2 and TOL plasmids 有权
标题翻译：基于RK2和TOL质粒的表达载体
公开(公告)号：US06258565B1
公开(公告)日：2001-07-10
申请号：US09242945
申请日：1999-09-10
申请人： Janet M. Blatny , Ponniah Karunakaran , Svein Valla
发明人： Janet M. Blatny , Ponniah Karunakaran , Svein Valla
IPC分类号： C12P2104
CPC分类号： C12N15/70 , C12N9/92 , C12N15/68 , C12N15/69 , C12N15/74
摘要： The present invention provides an expression vector comprising an RK2 minimum replicon together with an expression cassette comprising the regulatory functions of a TOL plasmid, and, in particular, an expression vector comprising a RK2 minimum replicon together with a promoter Pm and/or Pu and a corresponding regulatory gene xylS and/or xylR as derived from a TOL plasmid. Such expression vectors may be used to express desired genes in a wide range of gram negative and gram positive bacterial hosts.
摘要翻译：本发明提供包含RK2最小复制子的表达载体以及包含TOL质粒的调节功能的表达盒，特别是包含RK2最小复制子与启动子Pm和/或Pu和来自TOL质粒的相应的调控基因xylS和/或xylR。这样的表达载体可以用于在广泛的革兰氏阴性和革兰氏阳性细菌宿主中表达所需的基因。

64. 发明授权

US06207418B1 Production of a multimeric protein by cell fusion method 有权
标题翻译：通过细胞融合法制备多聚体蛋白
公开(公告)号：US06207418B1
公开(公告)日：2001-03-27
申请号：US09342301
申请日：1999-06-29
申请人： Nobuaki Hori , Claude Geoffrey Davis , Krisztina M. Zsebo , Aya Jakobovits
发明人： Nobuaki Hori , Claude Geoffrey Davis , Krisztina M. Zsebo , Aya Jakobovits
IPC分类号： C12P2104
CPC分类号： C07K16/244 , C07K14/00 , C07K16/00 , C07K2317/21 , C12N5/16 , C12N5/166 , C12N2510/02
摘要： The present invention features a method of producing a multimeric protein from a hybrid cell formed from the fusion of two or more cells, each of which cell is engineered to express one component of the multimeric protein, as well as a method for screening for successful fusion of the cells to produce a desired hybrid cell. The methods of the invention are widely applicable to the production of proteins having two or more components.
摘要翻译：本发明的特征在于从由两个或多个细胞的融合形成的杂交细胞产生多聚体蛋白质的方法，每个细胞被工程化以表达多聚体蛋白质的一个组分，以及筛选成功融合的方法的细胞以产生所需的杂交细胞。本发明的方法可广泛用于生产具有两种或多种组分的蛋白质。

65. 发明授权

US06180371B2 Modified factor VIII 失效
标题翻译：修改因子VIII
公开(公告)号：US06180371B2
公开(公告)日：2001-01-30
申请号：US09037601
申请日：1998-03-10
申请人： John S. Lollar
发明人： John S. Lollar
IPC分类号： C12P2104
CPC分类号： C07K14/755 , A61K38/00 , C07K2319/00
摘要： Specific amino acid loci of human factor VIII interact with inhibitory antibodies of hemophilia patients who have developed such antibodies after being treated with factor VIII. Modified factor VIII is disclosed in which the amino acid sequence is changed by a substitution at one or more of the specific loci. The modified factor VIII is not inhibited by inhibitory antibodies against the A2 or C2 domain epitopes. The modified factor VIII is useful for hemophiliacs, either to avoid or prevent the action of inhibitory antibodies.
摘要翻译：人因子VIII的特异性氨基酸位点与用因子VIII处理后发展了这种抗体的血友病患者的抑制性抗体相互作用。公开了修饰因子VIII，其中通过在一个或多个特定基因座处的取代改变氨基酸序列。修饰因子VIII不受针对A2或C2结构域表位的抑制性抗体的抑制。修饰的因子VIII可用于血友病患者，以避免或预防抑制性抗体的作用。

66. 发明授权

US06833256B1 Interferon tau mutants and methods for making them 失效
标题翻译：干扰素tau突变体及其制备方法
公开(公告)号：US06833256B1
公开(公告)日：2004-12-21
申请号：US09599413
申请日：2000-06-22
申请人： Carol H. Pontzer , Lynnette H. Shorts , Christina Dancz Clark
发明人： Carol H. Pontzer , Lynnette H. Shorts , Christina Dancz Clark
IPC分类号： C12P2104
CPC分类号： C07K14/555 , A61K38/00
摘要： The present invention is directed to the field of animal and human health, and more particularly to pharmacological uses of analogs or mutants of interferon-tau (IFN-&tgr;) that differ from native IFN-&tgr; because of substitutions of amino acids near the amino terminus of the IFN-&tgr; molecule that impart improved biological activity. The IFN-&tgr; mutants described in this disclosure have low toxicity, retain the same or slightly reduced antiviral activity compared with highly effective IFN-alpha, and have enhanced antiproliferative activity compared to native IFN-tau, making them useful in treating viral infections, cancer, and immune system diseases including autoimmune diseases. The present invention is also directed to a method for making novel recombinant proteins, especially interferons, interleukins, and cytokines, polypeptide hormones and other biopharmaceuticals that have improved biological activity over known proteins and/or lower toxicity and/or increased stability.
摘要翻译：本发明涉及动物和人类健康领域，更具体地涉及由于氨基末端附近的氨基酸取代而与天然IFN-τ不同的干扰素-τ（IFN-τ）的类似物或突变体的药理学用途的IFN-τ分子，其赋予改善的生物活性。在本公开中描述的IFN-τ突变体具有低毒性，与高效IFN-α相比保持相同或略微降低的抗病毒活性，并且与天然IFN-τ相比具有增强的抗增殖活性，使其可用于治疗病毒感染，癌症和免疫系统疾病，包括自身免疫性疾病。本发明还涉及一种制备新型重组蛋白质，特别是干扰素，白细胞介素和细胞因子，多肽激素和其它生物活性比已知蛋白质改善和/或降低毒性和/或增加的稳定性的生物药物的方法。

67. 发明授权

US06753166B2 IL-1 eta DNA and polypeptides 失效
标题翻译： IL-1 eta DNA和多肽
公开(公告)号：US06753166B2
公开(公告)日：2004-06-22
申请号：US09976472
申请日：2001-10-11
申请人： John E. Sims , Blair R. Renshaw
发明人： John E. Sims , Blair R. Renshaw
IPC分类号： C12P2104
CPC分类号： C07K14/545
摘要： The invention is directed to novel, purified and isolated IL-1 eta polypeptides and fragments thereof, the polynucleotides encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, peptides derived from these polypeptides, and uses thereof.
摘要翻译：本发明涉及新颖的，纯化和分离的IL-1 eta多肽及其片段，编码此类多肽的多核苷酸，产生此类多肽的重组形式的方法，针对这些多肽产生的抗体，衍生自这些多肽的肽及其用途。

68. 发明授权

US06703226B1 Expression of a foamy virus envelope protein 失效
标题翻译：泡沫病毒包膜蛋白的表达
公开(公告)号：US06703226B1
公开(公告)日：2004-03-09
申请号：US09923317
申请日：2001-08-08
申请人： Dirk Lindemann , Axel Rethwilm
发明人： Dirk Lindemann , Axel Rethwilm
IPC分类号： C12P2104
CPC分类号： C12N15/86 , C07K14/005 , C07K2319/00 , C12N2740/13043 , C12N2740/13045 , C12N2740/17022 , C12N2810/40 , C12N2810/50 , C12N2810/6054
摘要： The invention concerns constructs for the expression of a protein comprising at least a modified FV envelope protein, the protein so obtained as well as the complementation cell line permitting the production of pseudotyped viral particle. It also concerns pharmaceutical composition comprising said particles and a method for treating a disease.
摘要翻译：本发明涉及用于表达包含至少修饰的FV包膜蛋白的蛋白质，如此获得的蛋白质以及允许产生假型病毒颗粒的互补细胞系的蛋白质的构建体。它还涉及包含所述颗粒的药物组合物和治疗疾病的方法。

69. 发明授权

US06623957B2 Secretion of T cell receptor fragments from recombinant host cells 失效
标题翻译：从重组宿主细胞分泌T细胞受体片段
公开(公告)号：US06623957B2
公开(公告)日：2003-09-23
申请号：US10162127
申请日：2002-06-04
申请人： Elizabeth S. Ward
发明人： Elizabeth S. Ward
IPC分类号： C12P2104
CPC分类号： C07K16/00 , A61K47/6827 , A61K47/6849 , C07K14/70503 , C07K14/7051 , C07K14/70514 , C07K14/70535 , C07K14/70539 , C07K2317/52 , C07K2317/53 , C07K2319/00
摘要： Variable domain murine T-cell receptor genes have been isolated and used to construct cloning and expression vectors. V&agr;, V&bgr;, and single chain V&agr;-V&bgr; fragments have been expressed as secreted domains in Escherichia coli using the vectors. The domains are secreted into the culture supernatant in milligram quantities. The single domains and the single chain T-cell receptors are folded into &bgr;-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted fragments may be useful for immunization to generate anti-clonotypic antibodies, in vaccination or for high resolution structural studies. The genes encoding these domains may also serve as templates for in vitro mutagenesis and improvement of affinities of the TCR fragments for their interaction with cognate peptide-MHC complexes.
摘要翻译：已经分离了可变结构域鼠T细胞受体基因，并用于构建克隆和表达载体。 Valpha，Vbeta和单链Valpha-Vbeta片段已经使用载体在大肠杆菌中表达为分泌结构域。以毫克量将结构域分泌到培养上清液中。单结构域和单链T细胞受体被折叠成类似于免疫球蛋白可变结构域的β-折叠片结构。分泌的片段可用于免疫以产生抗克隆型抗体，在疫苗接种或高分辨率结构研究中。编码这些结构域的基因也可以用作体外诱变和提高TCR片段与同源肽-MHC复合物相互作用的亲和力的模板。

70. 发明授权

US06617134B1 Dimer of molecular variant of apolipoprotein and processes for the production thereof 失效
标题翻译：载脂蛋白的分子变体的二聚体及其制备方法
公开(公告)号：US06617134B1
公开(公告)日：2003-09-09
申请号：US09259434
申请日：1999-03-01
申请人： Cesare Sirtori , Guido Franceschini , Lars Abrahmsén , Erik Holmgren , Mats Lake , Björn Nilsson , Joanna Chmielewska , Peter Lind
发明人： Cesare Sirtori , Guido Franceschini , Lars Abrahmsén , Erik Holmgren , Mats Lake , Björn Nilsson , Joanna Chmielewska , Peter Lind
IPC分类号： C12P2104
CPC分类号： C07K14/775 , A61K38/1709 , Y02A50/473
摘要： Substantially pure dimers of Apolipoprotein AI-Milano (APO-AI-M/APO AI-M) were isolated from plasma and characterized. Apolipoprotein AI-M dimer can also be produced in a recombinant Escherichia coli system. Pharmaceutical compositions comprising the ApoAI-AI-M/ApoAI-M are described. Patients with atherosclerosis or cardiovascular diseases can be treated with the dimer. Medicaments containing the dimer can also be used to prevent thrombosis in different clinical circumstances, both at the arterial and at the venous level. The dimer can also act as a prodrug for the monomer.
摘要翻译：从血浆中分离出载脂蛋白AI-Milano（APO-AI-M / APO AI-M）的基本纯二聚体并进行表征。载脂蛋白AI-M二聚体也可以在重组大肠杆菌系统中产生。描述了包含ApoAI-AI-M / ApoAI-M的药物组合物。可以用二聚体治疗动脉粥样硬化或心血管疾病患者。含有二聚体的药物也可用于在动脉和静脉水平的不同临床情况下预防血栓形成。二聚体也可以作为单体的前药。

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