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    • 61. 发明授权
    • Apparatus and method for measuring spectral characteristics of
fluorescent sample
    • 用于测量荧光样品光谱特性的装置和方法
    • US6020959A
    • 2000-02-01
    • US172127
    • 1998-10-14
    • Kenji Imura
    • Kenji Imura
    • G01J3/443G01J3/02G01J3/42G01J3/44G01N21/27G01N21/64
    • G01J3/4406G01J3/02G01J3/0254G01J3/027G01N21/6428G01N21/645G01N2021/6419G01N2021/6421G01N21/276G01N2201/065
    • An apparatus for measuring a spectral characteristic of a fluorescent sample, being provided with: a first illuminator for emitting a beam in a wavelength range including the ultraviolet spectrum; a second illuminator for emitting a beam in a wavelength range longer than a first cutoff wavelength; a spectral radiance factor measuring device for measuring first and second total spectral radiance factors of a fluorescent sample by illuminating the fluorescent sample by the first illumination device and second illumination device, respectively; a memory for storing weight coefficients for weighting first and second total spectral radiance factors; and a calculator for calculating a total spectral radiance factor of the fluorescent sample in accordance with the following equation using measured first and second total spectral radiance factors and a weight coefficient:B.sub.t (.lambda.)=A(.lambda.).multidot.Bt.sub.1 (.lambda.)+{1-A(.lambda.)}.multidot.Bt.sub.2 (.lambda.)wherein:Bt(.lambda.): Total spectral radiance factor of the fluorescent sampleA(.lambda.): Weight coefficientBt.sub.1 (.lambda.): First total spectral radiance factorBt.sub.2 (.lambda.): Second total spectral radiance factor.
    • 一种用于测量荧光样品的光谱特性的装置,设置有:用于发射包括紫外光谱的波长范围内的光束的第一照明器; 用于发射长于第一截止波长的波长范围的光束的第二照明器; 光谱辐射因子测量装置,用于分别通过由第一照明装置和第二照明装置照射荧光样品来测量荧光样品的第一和第二总光谱辐射系数; 用于存储用于加权第一和第二总光谱辐射因子的权重系数的存储器; 以及计算器,用于使用测量的第一和第二总光谱辐射系数和权重系数根据以下等式计算荧光样品的总光谱辐射系数:Bt(λ)= A(λ)xBt1(λ)+ {1 -A(λ)} xBt2(λ)其中:Bt(λ):荧光样品的总光谱辐射系数A(λ):重量系数Bt1(λ):第一总光谱辐射系数Bt2(λ):第二总光谱 辐射因子
    • 65. 发明授权
    • Apparatus for measuring two different fluorescences of a sample
    • 用于测量样品的两种不同荧光的装置
    • US3971951A
    • 1976-07-27
    • US513869
    • 1974-10-10
    • Katsuji RikukawaKenji Onogi
    • Katsuji RikukawaKenji Onogi
    • G01J1/58G01N21/27G01N21/64G02B21/16
    • G01N21/645G01N2021/6419G01N2021/6421G01N2021/6441G01N2021/6473G01N21/276
    • In apparatus, such as a fluorometric microscope, for measuring two different fluorescences in a sample, the sample is irradiated by light modulated at a first frequency and having a first wavelength for causing a first fluorescence and also is irradiated by light modulated at a second frequency different from the first frequency and having a second wavelength for causing a second fluorescence. A photoelectric element is responsive to the light emitted by the sample during both fluorescences for generating an electrical signal representative of the combined intensities of the two fluorescences. From this electrical signal, a demodulator derives first and second signal components having the first and second frequencies, respectively, and having amplitudes representative of the individual intensities of the first and second fluorescences, respectively.
    • 在诸如荧光显微镜的装置中,用于测量样品中的两种不同的荧光,通过以第一频率调制并具有用于引起第一荧光的第一波长的光照射样品,并且还以第二频率调制的光照射样品 与第一频率不同,并且具有用于引起第二荧光的第二波长。 光电元件响应于在两个荧光期间由样品发射的光,以产生代表两种荧光的组合强度的电信号。 从该电信号中,解调器分别导出具有第一和第二频率的第一和第二信号分量,并分别具有代表第一和第二荧光的各个强度的振幅。