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61. 发明授权

US07910364B2 Rapidly cleavable sumo fusion protein expression system for difficult to express proteins 有权
标题翻译：快速切割相容融合蛋白表达系统难以表达蛋白质
公开(公告)号：US07910364B2
公开(公告)日：2011-03-22
申请号：US12249334
申请日：2008-10-10
申请人： Christopher D. Lima , Elena Mossessova
发明人： Christopher D. Lima , Elena Mossessova
IPC分类号： C12N15/00 , C12N5/00 , C12P21/06 , C07H21/00
CPC分类号： C12N15/62 , C07K2319/02 , C07K2319/21 , C07K2319/23 , C07K2319/35 , C07K2319/43 , C07K2319/95
摘要： A recombinant expression system for the expression of a poly amino acid, peptide or protein is provided. The poly amino acid of interest is expressed as a fusion protein that includes an amino acid sequence recognized and cleaved by a Ulp1 protease. The amino acid sequence joined to the poly amino acid of interest is preferably from a SUMO (small ubiquitin-like molecule) protein. This sequence imparts favorable solubility and refolding properties to the fusion protein. A purification tag may also be incorporated into the fusion protein for ease of isolation. This recombinant expression system is particularly advantageous for expression and rapid and highly specific cleavage and purification of poly amino acids that have low solubilities or are difficult to express in other systems.
摘要翻译：提供了用于表达多氨基酸，肽或蛋白质的重组表达系统。感兴趣的多氨基酸被表达为融合蛋白，其包含由Ulp1蛋白酶识别并切割的氨基酸序列。与目的聚氨基酸连接的氨基酸序列优选为SUMO（小泛素样分子）蛋白质。该序列赋予融合蛋白有利的溶解度和重折叠性质。还可以将融合蛋白引入纯化标签以便于分离。这种重组表达系统特别有利于表达和快速且高度特异性地裂解和纯化具有低溶解性或难以在其它系统中表达的聚氨基酸。

62. 发明授权

US07910301B2 Methods for drug discovery, disease treatment, and diagnosis using metabolomics 有权
标题翻译：药物发现，疾病治疗和使用代谢组学诊断的方法
公开(公告)号：US07910301B2
公开(公告)日：2011-03-22
申请号：US11728826
申请日：2007-03-27
申请人： Rima Kaddurah-Daouk , Bruce S. Kristal
发明人： Rima Kaddurah-Daouk , Bruce S. Kristal
IPC分类号： C12Q1/00
CPC分类号： G01N33/5038 , G01N33/4833 , G01N33/5011 , G01N33/5079 , G01N33/5091 , G01N33/6893 , G01N2800/304 , G01N2800/52 , Y02A50/57 , Y02A90/26
摘要： The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.
摘要翻译：比较细胞的小分子轮廓以鉴定在改变状态下调节的小分子。还讨论了细胞小分子文库，鉴定组织来源的方法，用于治疗遗传和非遗传性疾病的方法以及预测药物功效的方法。

63. 发明授权

US07902321B2 One-pot, one-step in situ living polymerization from silicate anchored multifunctional initiator 有权
标题翻译：来自硅酸盐锚定多功能引发剂的一锅，一步原位活性聚合
公开(公告)号：US07902321B2
公开(公告)日：2011-03-08
申请号：US11665157
申请日：2005-11-09
申请人： Dotsevi Y. Sogah , Jianbo Di
发明人： Dotsevi Y. Sogah , Jianbo Di
IPC分类号： C08K3/34 , C08K3/20 , C08G63/48 , C08G63/91
CPC分类号： C08G63/823 , C08G63/00 , C08G65/10 , C08G77/442
摘要： Silicate anchored multifunctional initiator has moiety initiating ring opening living polymerization of lactone or ethylene oxide or cyclic siloxane monomer and other moiety for initiating living free radical polymerization of ethylenically unsaturated monomer. The monomers are reacted with the initiator in a one-pot, one-step reaction to cause living polymerization of both monomers and exfoliation of silicate layers to provide dispersed block copolymer silicate nanocomposite, with the junction of the two blocks being anchored to silicate layer and each block dangling therefrom.
摘要翻译：硅酸盐锚定多功能引发剂具有内酯或环氧乙烷或环状硅氧烷单体等部分引发开环活性聚合，用于引发烯属不饱和单体的活性自由基聚合。单体与引发剂在一锅一步反应中反应，引起单体的活性聚合和硅酸盐层的剥离，以提供分散的嵌段共聚物硅酸盐纳米复合材料，两个嵌段的连接被锚定到硅酸盐层和每个块悬挂在其中。

64. 发明授权

US07893233B2 Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays 有权
标题翻译：使用具有可寻址阵列的连接酶检测反应检测核酸序列差异
公开(公告)号：US07893233B2
公开(公告)日：2011-02-22
申请号：US12725230
申请日：2010-03-16
申请人： Francis Barany , George Barany , Robert P. Hammer , Maria Kempe , Herman Blok , Monib Zirvi
发明人： Francis Barany , George Barany , Robert P. Hammer , Maria Kempe , Herman Blok , Monib Zirvi
IPC分类号： C07H21/04 , C12N15/11
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00536 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00729 , B82Y30/00 , C12Q1/6816 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C40B40/06 , C40B60/14 , Y02A50/58 , C12Q2565/501 , C12Q2561/125 , C12Q2537/143 , C12Q2565/514
摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.
摘要翻译：本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。该方法包括连接阶段，捕获阶段和检测阶段。连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

65. 发明申请

US20110039729A1 GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES 有权
标题翻译：糖蛋白在蛋白质表达中的表达
公开(公告)号：US20110039729A1
公开(公告)日：2011-02-17
申请号：US12811788
申请日：2009-01-05
申请人： Matthew Delisa , Cassandra Guarino , Thomas Mansell , Adam Fisher
发明人： Matthew Delisa , Cassandra Guarino , Thomas Mansell , Adam Fisher
IPC分类号： C40B30/06 , C12N1/00 , C07K14/00 , C12P21/00 , C07K16/00
CPC分类号： C12N15/70 , C07K16/1278 , C07K2317/21 , C07K2317/41 , C12N9/1048 , C12N9/1051 , C12N9/14 , C12P21/005 , C12Y204/01132 , C12Y204/01142 , C12Y204/01255 , C12Y204/01257 , C12Y306/03001
摘要： The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.
摘要翻译：本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中真核糖基转移酶活性是真核的二酰基连接的UDP-GlcNAc转移酶活性和真核甘露糖基转移酶活性。还公开了通过提供包含真核糖基转移酶活性的原核宿主细胞并在有效产生糖基化蛋白的条件下培养原核宿主细胞来生产糖基化蛋白的方法。本发明的另一方面涉及通过在细菌表面上表达一种或多种聚糖以在细菌表面上或噬菌体的表面上附着一个或多个聚糖上的标记物来筛选细菌或噬菌体的方法衍生自细菌，并以高通量格式分析标签。还公开了包含识别和结合天然抗原的Fv部分的糖基化抗体和在保守的天冬酰胺残基处被糖基化的Fc部分。

66. 发明申请

US20110021885A1 SUBDURAL ELECTRO-OPTICAL SENSOR 有权
标题翻译：小型电光传感器
公开(公告)号：US20110021885A1
公开(公告)日：2011-01-27
申请号：US12598543
申请日：2008-05-05
申请人： Hongtao Ma , Theodore H. Schwartz , Jian-Young Wu
发明人： Hongtao Ma , Theodore H. Schwartz , Jian-Young Wu
IPC分类号： A61B5/00
CPC分类号： A61B5/0086 , A61B5/0084 , A61B5/4076 , A61B2562/0233 , A61B2562/046
摘要： A subdural electro-optical sensor system may include a substrate to which is attached an array of electrodes, light emitters, and light detectors. The sensor system may be sufficiently thin, flexible, sterile and biocompatible to be positioned subdurally.
摘要翻译：硬膜下电光传感器系统可以包括附接有电极阵列，发光体和光检测器的基底。传感器系统可以是足够薄的，柔性的，无菌的和生物相容的，以便被硬膜定位。

67. 发明授权

US07867335B2 GaN bulk growth by Ga vapor transport 有权
标题翻译：通过Ga蒸汽输送生长GaN
公开(公告)号：US07867335B2
公开(公告)日：2011-01-11
申请号：US11541919
申请日：2006-10-02
申请人： Michael G. Spencer , Phani Konkapaka , Huaqiang Wu , Yuri Makarov
发明人： Michael G. Spencer , Phani Konkapaka , Huaqiang Wu , Yuri Makarov
IPC分类号： C30B21/02
CPC分类号： C30B25/00 , C30B23/00 , C30B29/40 , C30B29/406 , H01L21/02389 , H01L21/0242 , H01L21/0254 , H01L21/0262 , H01L21/02631 , H01L31/184
摘要： GaN is grown by creating a Ga vapor from a powder, and using an inert purge gas from a source to transport the vapor to a growth site where the GaN growth takes place. In one embodiment, the inert gas is N2, and the powder source is GaN powder that is loaded into source chambers. The GaN powder is congruently evaporated into Ga and N2 vapors at temperatures between approximately 1000 and 1200° C. The formation of Ga liquid in the powder is suppressed by the purging of an inert gas through the powder. The poser may also be isolated from a nitride containing gas provided at the growth cite. In one embodiment, the inert gas is flowed through the powder.
摘要翻译：通过从粉末中产生Ga蒸气并使用来自源的惰性吹扫气体将蒸汽输送到发生GaN生长的生长位置来生长GaN。在一个实施方案中，惰性气体是N 2，并且粉末源是装载到源室中的GaN粉末。在大约1000-1200℃的温度下，GaN粉末被一致地蒸发成Ga和N 2蒸气。通过粉末中的惰性气体的吹扫来抑制粉末中的Ga液的形成。该位置也可以与生长引起的含氮气体隔离。在一个实施方案中，惰性气体流过粉末。

68. 发明申请

US20100330043A1 GPR125 AS A MARKER FOR STEM AND PROGENITOR CELLS AND METHODS USE THEREOF 审中-公开
标题翻译： GPR125作为干细胞和祖细胞的标记物及其使用方法
公开(公告)号：US20100330043A1
公开(公告)日：2010-12-30
申请号：US12446730
申请日：2007-10-23
申请人： Shahin Rafii , Sergey V. Shmelkov , Marco Seandel , Sai H. Chavala
发明人： Shahin Rafii , Sergey V. Shmelkov , Marco Seandel , Sai H. Chavala
IPC分类号： A61K35/12 , C12N5/074 , G01N33/53 , A61P35/00
CPC分类号： C12N5/061 , A61K35/12 , C12N5/0623 , C12N5/068 , C12N2501/11 , C12N2501/115 , C12N2501/13 , C12N2501/235 , C12N2501/998 , C12N2502/02 , C12N2502/04 , G01N33/56966 , G01N33/57492
摘要： The present invention relates to GPR1 25 as a marker of stem and progenitor cells, including multipotent adult spermatogonial-derived stem cells (MASCs), spermatogonial stem and progenitor cells, skin stem or progenitor cells, intestinal stem or progenitor cells, neural stem or progenitor cells, and cancer stem cells. The invention provides, inter alia, methods for enriching or isolating GPR125-positive stem or progenitor cells, methods for detecting GPR125-positive stem or progenitor cells, methods for culturing GPR125-positive stem or progenitor cells, purified GPR125-positive stem or progenitor cells, therapeutic compositions containing purified GPR125-positive stem or progenitor cells, methods for targeting therapeutic agents to GPR125-positive stem and progenitor cells, and methods of treatment comprising administering GPR125-positive stem and progenitor cells, or differentiated cells derived therefrom, to subjects in need thereof. The present invention also provides methods of detecting cancer cells based on GPR1 25 expression, and methods of targeting therapeutic agents to cancer cells to GPR125-positive cancer cells.
摘要翻译：本发明涉及GPR125作为干细胞和祖细胞的标志物，包括多能成人精原细胞来源的干细胞（MASC），精原干细胞和祖细胞，皮肤干细胞或祖细胞，肠干或祖细胞，神经干或祖细胞细胞和癌症干细胞。本发明尤其提供了富集或分离GPR125阳性干细胞或祖细胞的方法，用于检测GPR125阳性干细胞或祖细胞的方法，培养GPR125阳性干细胞或祖细胞，纯化的GPR125阳性干细胞或祖细胞含有纯化的GPR125阳性干细胞或祖细胞的治疗组合物，用于将治疗剂靶向GPR125阳性干细胞和祖细胞的方法，以及治疗方法，包括将GPR125阳性干细胞和祖细胞或其衍生的分化细胞给予受试者需要。本发明还提供了基于GPR125表达检测癌细胞的方法，以及将治疗剂靶向癌细胞至GPR125阳性癌细胞的方法。

69. 发明授权

US07839775B2 Methods and systems for channel sensing multiple access communications with multipacket reception 有权
标题翻译：用于信道感测与多包接收多址通信的方法和系统
公开(公告)号：US07839775B2
公开(公告)日：2010-11-23
申请号：US11376067
申请日：2006-03-14
申请人： Douglas S. Chan , Toby Berger
发明人： Douglas S. Chan , Toby Berger
IPC分类号： H04W4/00 , H04J1/16 , H04J3/14
CPC分类号： H04W74/0808 , H04L1/0001 , H04L1/002
摘要： Methods and Systems for communication using carrier sense multiple access (CSMA) with multipacket reception (MPR).
摘要翻译：使用具有多段接收（MPR）的载波侦听多路访问（CSMA）进行通信的方法和系统。

70. 发明授权

US07835055B2 Fiber based MEMS 有权
标题翻译：基于光纤的MEMS
公开(公告)号：US07835055B2
公开(公告)日：2010-11-16
申请号：US12615625
申请日：2009-11-10
申请人： Shahyaan Desai
发明人： Shahyaan Desai
IPC分类号： G02B26/08
CPC分类号： B82Y20/00 , B82Y30/00 , G02B6/3512 , G02B6/3566 , G02B6/357 , G02B6/3572 , G02B6/3578 , G02B6/3584 , G02B26/0833 , Y10T29/49826
摘要： MEMS can be fabricated from fibers without the use of a matrix material. Devices can be built where fibers are attached only at a substrate edge (e.g. cantilevers, bridges). Motions can be controlled by adjusting the linkage between multiple fibers with weak coupling (e.g. base, tip, in-between). Driving mechanisms include base-forcing (magnetics, piezo, electrostatics) or tip forcing (magnetics). Mirrors may be formed on free ends of cantilevers to form optical scanners.
摘要翻译： MEMS可以由不使用基质材料的纤维制成。可以在仅在基板边缘（例如悬臂梁，桥梁）附接纤维的地方构建装置。可以通过调节具有弱耦合的多个纤维之间的连接（例如底部，尖端，中间）来控制运动。驱动机构包括强制（磁性，压电，静电）或尖端强制（磁性）。可以在悬臂的自由端上形成镜子以形成光学扫描器。

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