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    • 63. 发明申请
    • METHOD FOR MEASURING CYSTATIN C IN HUMAN BODY FLUID
    • 人体体液中CYSTATIN C的测定方法
    • US20110236996A1
    • 2011-09-29
    • US13132790
    • 2009-12-03
    • Shinya NakayamaHiroshi TakahashiYasushi NakamuraTomo Shimizu
    • Shinya NakayamaHiroshi TakahashiYasushi NakamuraTomo Shimizu
    • G01N33/68
    • G01N33/543G01N33/6893G01N2333/8139
    • There is provided a particle-enhanced immunoassay method for cystatin C in a human body fluid, which has higher specificity and is easily automatable at low cost as compared with conventional assay methods that use large amounts of polyclonal antibodies having low specificity or monoclonal antibodies having high specificity but having poor agglutinability.Disclosed is an assay method specific to cystatin C in a human body fluid, which is a particle-enhanced immunoassay method utilizes a combination of antibody-coated particles in which some insoluble carrier particles are coated with anti-human cystatin C monoclonal antibodies of one type which have high affinity and in which some other insoluble carrier particles are coated with anti-human cystatin C monoclonal antibodies of one type which recognize an epitope different from that of the monoclonal antibody of the first-mentioned type and which have relatively low affinity, wherein the binding amount of the anti-human cystatin C monoclonal antibodies relative to the total weight of the particles being coated with the corresponding antibodies is less than 5% by weight.
    • 与使用大量具有低特异性的多克隆抗体或具有高特异性的单克隆抗体的常规测定方法相比,提供了人体液中半胱氨酸蛋白酶抑制剂C的微粒增强免疫测定方法,其具有更高的特异性并且可以以低成本容易地自动化 特异性但具有差的凝集性。 本发明公开了一种人体液中半胱氨酸蛋白酶抑制剂C特异性检测方法,其是使用抗体包被的颗粒的组合,其中一些不溶性载体颗粒被一种类型的抗人半胱氨酸蛋白酶抑制剂C单克隆抗体包被, 其具有高亲和力,并且其中一些其它不溶性载体颗粒被一种类型的抗人半胱氨酸蛋白酶抑制剂C单克隆抗体包被,所述抗人半胱氨酸蛋白酶抑制剂C单克隆抗体识别与第一类型的单克隆抗体的表位不同并具有相对低亲和力的表位,其中 抗人半胱氨酸蛋白酶抑制剂C单克隆抗体相对于被相应抗体包被的颗粒的总重量的结合量小于5重量%。