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61. 发明授权

US4865976A Method of cyclodextrin manufacture using an immobilized cyclodextrin glycosyltransferase 失效
标题翻译：使用固定化环糊精糖基转移酶制备环糊精的方法
公开(公告)号：US4865976A
公开(公告)日：1989-09-12
申请号：US905599
申请日：1986-09-10
申请人： Ronald P. Rohrbach , Dale S. Scherl
发明人： Ronald P. Rohrbach , Dale S. Scherl
IPC分类号： C12P19/18
CPC分类号： C12P19/18
摘要： A process for the continuous production of cyclodextrins employs as a feedstock an aqueous solution of partially hydrolyzed starch with a dextrose equivalent between about 10 and about 15 as the feedstock for an immobilized cyclodextrin glycosyltransferase. In one variant the feedstock is sent to a membrane which removes cyclodextrins and glucose and the retentate is recycled to an immobilized glycosyltransferase so as to effectively utilize the feedstock. In another variant the effluent is passed through a water immiscibled liquid organic compound which forms a solid insoluble complex with cyclodextrins, the solids are continually removed, and the aqueous phase is recycled to an immobilized cyclodextrin glycosyltransferase as in the prior variant.
摘要翻译：用于连续生产环糊精的方法使用具有约10至约15的葡萄糖当量的部分水解淀粉的水溶液作为原料，作为固定化环糊精糖基转移酶的原料。在一个变体中，将原料送入除去环糊精和葡萄糖的膜，并将滞留物再循环至固定的糖基转移酶，以便有效地利用原料。在另一个变体中，流出物通过不溶于水的液体有机化合物，其与环糊精形成固体不溶性络合物，连续除去固体，并将水相如先前变体一样再循环至固定的环糊精糖基转移酶。

62. 发明授权

US4748237A Increasing cyclodextrin yields by the addition of various solutes to starch feedstocks 失效
标题翻译：通过向淀粉原料中加入各种溶质来增加环糊精产率
公开(公告)号：US4748237A
公开(公告)日：1988-05-31
申请号：US36725
申请日：1987-04-10
申请人： Ronald P. Rohrbach , Dale S. Scherl
发明人： Ronald P. Rohrbach , Dale S. Scherl
IPC分类号： C08B37/16 , C12P19/18
CPC分类号： C08B37/0012 , C12P19/18
摘要： The yield of cyclodextrins formed in the hydrolysis of starch catalyzed by a cyclodextrin glycosyltransferase can be substantially increased by the addition of certain water-soluble solutes. Alcohols containing 1 to 6 carbon atoms form one such class, with ethanol and isopropyl alcohol being especially effective. Where an immobilized cyclodextrin glycosyltransferase is used yields of beta-cyclodextrin can be increased from approximately 20% to almost 40% using 15% isopropyl alcohol in a thinned starch feedstock, with glucose production being increased by only about 25%.
摘要翻译：在环糊精糖基转移酶催化的淀粉水解中形成的环糊精的产率可以通过加入某些水溶性溶质而显着提高。含有1至6个碳原子的醇形成一类，其中乙醇和异丙醇是特别有效的。在使用固定化环糊精糖基转移酶的情况下，使用15％异丙醇的稀释淀粉原料中β-环糊精的产率可从约20％增加至近40％，葡萄糖产量仅增加约25％。

63. 发明授权

US4250260A Regeneration of an immobilized enzyme system 失效
标题翻译：固定化酶体系的再生
公开(公告)号：US4250260A
公开(公告)日：1981-02-10
申请号：US68803
申请日：1979-08-22
申请人： Ronald P. Rohrbach , Joseph Levy
发明人： Ronald P. Rohrbach , Joseph Levy
IPC分类号： C12N11/08 , C12N11/14 , C12P19/20 , C12P19/24 , B01J31/40
CPC分类号： C12N11/14 , C12N11/08 , C12P19/20 , C12P19/24
摘要： A method for regenerating an immobilized enzyme system comprises treating the system with an acid, removing excess acid, treating the system with a bifunctional organic reagent which furnishes a pendant group, removing excess of said bifunctional reagent, and immobilizing fresh, active enzyme.
摘要翻译：用于再生固定化酶体系的方法包括用酸处理体系，除去过量的酸，用提供侧基的双官能有机试剂处理系统，除去过量的所述双功能试剂，并固定新鲜的活性酶。

64. 发明授权

US4250080A Preparation of support matrices for immobilized enzymes 失效
标题翻译：固定化酶支持基质的制备
公开(公告)号：US4250080A
公开(公告)日：1981-02-10
申请号：US91928
申请日：1979-11-07
申请人： Ronald P. Rohrbach , George W. Lester
发明人： Ronald P. Rohrbach , George W. Lester
IPC分类号： C12N11/08 , C12N11/14 , C08K3/00
CPC分类号： C12N11/08 , C12N11/14
摘要： Support matrices for immobilized enzymes may be prepared by treating a solid porous, inorganic, water-insoluble support such as an alumina with a prepolymerized polymeric compound such as polystyrene. After treating the solid support the resultant organic-inorganic composite may then be derivatized, one example of derivatization being nitration of the composite with nitric acid and reducing the nitro-substituted composite to form an aminopolystyrene alumina composite. This composite is then treated with a bifunctional monomer to form a copolymeric material which is substantially entrapped in the pores of the solid support, said copolymeric material containing functionalized pendent groups to which an enzyme may be coupled to form an immobilized enzyme conjugate.
摘要翻译：固定化酶的支撑基质可以通过用预聚合的聚合物如聚苯乙烯处理固体多孔，无机，水不溶性载体如氧化铝来制备。在处理固体支持物之后，所得到的有机 - 无机复合物可以被衍生化，衍生化的一个实例是用硝酸硝化复合物并还原硝基取代的复合物以形成氨基聚苯乙烯氧化铝复合材料。然后用双官能单体处理该复合物以形成基本上夹带在固体载体的孔中的共聚物，所述共聚物含有官能化的侧基，其中酶可与之偶联以形成固定化的酶偶联物。

65. 发明授权

US5286767A Modified agar and process for preparing modified agar for use ceramic composition to add green strength and/or improve other properties of a preform 失效
标题翻译：改性琼脂和用于制备用于使用陶瓷组合物的改性琼脂以增加生坯强度和/或改善预成型件的其它性能的方法
公开(公告)号：US5286767A
公开(公告)日：1994-02-15
申请号：US676646
申请日：1991-03-28
申请人： Ronald P. Rohrbach , Julie R. Schollmeyer , Anthony J. Fanelli
发明人： Ronald P. Rohrbach , Julie R. Schollmeyer , Anthony J. Fanelli
IPC分类号： B22F3/02 , B22F1/00 , B22F3/22 , C04B35/622 , C04B35/632 , C04B35/636 , C08L5/12 , B09C39/00
CPC分类号： B22F3/225 , B22F1/0059 , B22F3/22 , C04B35/636 , B22F2001/0066 , B22F2998/00
摘要： Novel gel-forming compositions are used in a process for molding ceramic compositions. Gel-forming compositions, such as agar, are improved with a gel-enhancing agent, e.g. polyethyleneimine. The novel gel-forming compositions improved the ceramic molding process and/or the ceramic products formed therefrom.
摘要翻译：新型凝胶形成组合物用于模制陶瓷组合物的方法中。凝胶形成组合物，例如琼脂，用凝胶增强剂，例如，聚乙烯亚胺。新型凝胶形成组合物改善了陶瓷成型工艺和/或由其形成的陶瓷产品。

66. 发明授权

US4511654A Production of high sugar syrups 失效
标题翻译：生产高糖糖浆
公开(公告)号：US4511654A
公开(公告)日：1985-04-16
申请号：US499652
申请日：1983-05-31
申请人： Ronald P. Rohrbach , Mary J. Maliarik , Thomas P. Malloy
发明人： Ronald P. Rohrbach , Mary J. Maliarik , Thomas P. Malloy
IPC分类号： C12P19/20 , C12P19/22 , C12N11/14
CPC分类号： C12P19/22 , C12P19/20
摘要： The preparation of syrups which contain a high glucose or maltose content may be effected in a two-step process. In the first step, a feedstock such as starch which has been pretreated with alpha-amylase to adjust the dextrose equivalent is contacted with an enzyme such as amyloglucosidase or beta-amylase immobilized on a solid support. The contact time is adjusted by correlating the residence time and the liquid hourly space velocity so as to provide a conversion percentage of starch in the range of from about 50% to 85%, thus avoiding the formation of undesired conversion products. The partially hydrolyzed reaction mixture from the enzyme treatment is then passed through an ultrafiltration membrane whereby the permeate comprising the high glucose or maltose content is recovered, while the retentate is recycled for admixture with the partially hydrolyzed reaction mixture or for further treatment with the immobilized enzyme.
摘要翻译：含有高葡萄糖或麦芽糖含量的糖浆的制备可以两步法进行。在第一步中，将已经用α-淀粉酶预处理以调节葡萄糖当量的原料如淀粉与固定在固体支持物上的酶如淀粉葡糖苷酶或β-淀粉酶接触。通过将停留时间和液体时空速相关联来调节接触时间，从而提供约50％至85％范围内淀粉的转化率，从而避免形成不期望的转化产物。然后将来自酶处理的部分水解的反应混合物通过超滤膜，由此回收包含高葡萄糖或麦芽糖含量的渗透物，同时将渗余物再循环用于与部分水解的反应混合物混合或用固定化酶进一步处理。

67. 发明授权

US4415656A Increasing the stability of amyloglucosidase 失效
标题翻译：提高淀粉葡萄糖苷酶的稳定性
公开(公告)号：US4415656A
公开(公告)日：1983-11-15
申请号：US380797
申请日：1982-05-21
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： C12N9/00 , C12N9/34 , C12P19/20
CPC分类号： C12N9/2428 , C12N9/00 , C12P19/20
摘要： Thermal deactivation of amyloglucosidase is substantially retarded at elevated pressures relative to atmospheric pressure. The productivity of glucose formation from amyloglucosidase-catalyzed hydrolysis of starch or partially hydrolyzed starch is significantly increased when the hydrolysis is performed at a pressure greater than about 500 psig.
摘要翻译：淀粉葡糖苷酶的热失活在相对于大气压的升高的压力下基本上延迟。当在大约500psig的压力下进行水解时，由淀粉或部分水解淀粉的淀粉葡糖苷酶催化的水解产生葡萄糖的生产率显着增加。

68. 发明授权

US4382121A Pretreatment of glucose feedstock 失效
标题翻译：葡萄糖原料的预处理
公开(公告)号：US4382121A
公开(公告)日：1983-05-03
申请号：US261995
申请日：1981-05-08
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： C12P19/24 , C12P7/00
CPC分类号： C12P19/24
摘要： Commercial glucose solutions used as feedstock for enzymatic conversion of glucose to fructose by glucose isomerase often contain materials which act as poisons toward the enzyme. It has been found that these poisons can be removed, or destroyed, by treatment of the feedstock with oxidizing agents, chief of which is hydrogen peroxide.
摘要翻译：用作葡萄糖异构酶将葡萄糖酶转化为果糖的原料的商业葡萄糖溶液通常含有作为对该酶的毒物的物质。已经发现，通过用主要是过氧化氢的氧化剂处理原料，可以除去或破坏这些毒素。

69. 发明授权

US4292199A Method of preparing a support matrix 失效
标题翻译：制备支撑体的方法
公开(公告)号：US4292199A
公开(公告)日：1981-09-29
申请号：US178659
申请日：1980-08-18
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： B01J31/00 , B01J31/02 , B01J31/06 , C12N11/08 , C12N11/14
CPC分类号： B01J31/0254 , B01J31/003 , B01J31/06 , C12N11/08 , C12N11/14
摘要： An improved method of preparing support matrices, characterized as inorganic oxides impregnated with a polyamine cross-linked with a bifunctional reagent so as to furnish a plurality of pendant functional groups, comprises impregnating the oxide at a pH less than about 4 and subsequently curing the polyamine-impregnated oxide at a pH from about 8 to about 12. This improved method leads to subsequently prepared immobilized enzyme systems whose activity is increased by about 35%.
摘要翻译：一种改进的制备支撑基质的方法，其特征在于用双官能试剂交联的多胺浸渍的无机氧化物，以便提供多个侧链官能团，包括在小于约4的pH下浸渍氧化物，随后固化多胺在约8至约12的pH下的浸渍氧化物。该改进的方法导致随后制备的活性增加约35％的固定化酶体系。

70. 发明授权

US4268419A Support matrices for immobilized enzymes 失效
标题翻译：支持固定化酶的基质
公开(公告)号：US4268419A
公开(公告)日：1981-05-19
申请号：US95020
申请日：1979-11-16
申请人： Ronald P. Rohrbach
发明人： Ronald P. Rohrbach
IPC分类号： C12N11/08 , C12N11/14 , B01J31/06
CPC分类号： C12N11/14 , C12N11/08
摘要： An improved method of preparing support matrices for immobilization of reactive chemical entities, such as enzymes, comprises deposition of a polyamine on core support, such as an inorganic oxide, contacting the polyamine-coated core support with a bifunctional reagent which cross-links the polyamine and provides pendant functional groups, and recovering the matrix, wherein the improvement comprises means of depositing the polyamine as a thin, uniform film.
摘要翻译：制备用于固定化学反应活性化合物例如酶的支持基质的改进方法包括将多胺沉积在核载体如无机氧化物上，使多胺包被的核心载体与交联多胺的双官能试剂接触并提供侧链官能团和回收基质，其中改进包括将多胺沉积为薄均匀的膜的方法。

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