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61. 发明授权

US07713474B2 Liquid permeable composition in dry reagent devices 有权
标题翻译：干燥试剂装置中的透液性组合物
公开(公告)号：US07713474B2
公开(公告)日：2010-05-11
申请号：US10966858
申请日：2004-10-14
申请人： Lloyd S. Schulman , Michael J. Pugia , Spencer Lin , Karlheinz Hildenbrand
发明人： Lloyd S. Schulman , Michael J. Pugia , Spencer Lin , Karlheinz Hildenbrand
IPC分类号： G01N31/22 , G01N31/00
CPC分类号： G01N33/54393 , G01N33/525
摘要： A device for detecting an analyte in a fluid sample includes a liquid permeable composition for making a physical separation between compositions of the sample or for reacting with components. The liquid permeable composition has adhesive properties and can be used to make a multi-layered test strip or the composition can be used in the sample wells of microfluidic devices.
摘要翻译：用于检测流体样品中的分析物的装置包括用于在样品组合物之间进行物理分离或与组分反应的液体可渗透组合物。液体可渗透组合物具有粘合性能，并且可用于制备多层测试条，或者该组合物可用于微流体装置的样品孔中。

62. 发明授权

US06306660B1 Method for improving the accuracy of the semi-quantitative determination of analyte in fluid samples 有权
标题翻译：提高流体样品中分析物半定量测定精度的方法
公开(公告)号：US06306660B1
公开(公告)日：2001-10-23
申请号：US09484293
申请日：2000-01-18
申请人： Koleen K. Messenger , Michael J. Pugia , Jane F. Wallace
发明人： Koleen K. Messenger , Michael J. Pugia , Jane F. Wallace
IPC分类号： G01N3300
CPC分类号： G01N33/52 , Y10T436/105831
摘要： Disclosed is an improved method for determining the concentration of a first analyte in a fluid test sample as a function of a second analyte also present in the sample whose concentration in the fluid sample is clinically related to that of the first analyte. The method involves determining the concentration of the first analyte, and, if this concentration is outside of its useful analytical range, dividing this concentration by the normal concentration of the second analyte. This method of ratioing the concentrations of the first and second analyte is advantageous because accuracy is increased with fewer false positive and false negative results being reported.
摘要翻译：公开了一种用于确定流体测试样品中第一分析物的浓度作为也存在于样品中的第二分析物的函数的改进方法，其中流体样品中的浓度与第一分析物的浓度临床相关。该方法包括确定第一分析物的浓度，并且如果该浓度超出其有用的分析范围，则将该浓度除以第二分析物的正常浓度。这种比较第一和第二分析物的浓度的方法是有利的，因为精确度增加，报告的假阳性和假阴性结果较少。

63. 发明授权

US06228602B1 Competitive apo-peroxidase assay 失效
标题翻译：竞争性过氧化物酶测定
公开(公告)号：US06228602B1
公开(公告)日：2001-05-08
申请号：US09415491
申请日：1999-10-12
申请人： Michael J. Pugia
发明人： Michael J. Pugia
IPC分类号： G01N33554
CPC分类号： G01N33/6854 , C12Q1/04 , C12Q1/28 , G01N33/53
摘要： Disclosed is an assay for an analyte in a fluid test sample such as urine which involves combining the fluid test sample with a reagent system comprising an apo-peroxidase, a redox dye, a peroxide and a metal porphyrin which is bound to an analyte/analyte specific binding partner which complex has a combined molecular weight of at least about 180 K Daltons. When this conjugate interacts with analyte in the fluid test sample, a portion of the specific binding partner is dissociated from the complex thereby enabling the metal porphyrin to reconstitute with the apo-peroxidase. The reconstituted peroxidase can interact with the peroxide and redox dye to provide a colored response to analyte in the fluid test sample.
摘要翻译：公开了一种流体测试样品如尿液中的分析物的测定方法，其包括将流体测试样品与包含过氧化物酶，氧化还原染料，过氧化物和金属卟啉的试剂系统组合，所述过氧化物和金属卟啉与分析物/分析物结合该复合物具有至少约180K道尔顿的组合分子量的特异性结合配偶体。当该共轭体与流体测试样品中的分析物相互作用时，一部分特异性结合配偶体与络合物解离，从而使金属卟啉与载脂蛋白过氧化物酶重组。重构的过氧化物酶可以与过氧化物和氧化还原染料相互作用，以提供流体测试样品中分析物的着色响应。

64. 发明授权

US5846754A Enzyme determination with protease inactivation 失效
标题翻译：用蛋白酶灭活酶测定
公开(公告)号：US5846754A
公开(公告)日：1998-12-08
申请号：US653865
申请日：1996-05-28
申请人： Michael J. Pugia , Robert J. Schaeper
发明人： Michael J. Pugia , Robert J. Schaeper
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/37 , C12Q1/44 , C12Q1/54
CPC分类号： C12Q1/44 , C12Q1/37 , C12Q2334/00 , C12Q2334/70 , G01N2333/966
摘要： The present invention involves a composition and method for the determination of enzymatic activity or enzyme substrate concentration in a fluid test sample in which the enzyme catalyzes a reaction in a reagent system to provide a detectable response indicative of the concentration of the enzyme or enzyme substrate in the test sample. A measured amount of a protease capable of eliminating the enzymatic activity is included in the reagent composition, so that when a measured amount of test sample is treated, the reaction producing the detectable response will have a definite end point. The invention is particularly useful in conjunction with the determination of leucocytes in urine wherein the leukocytes are lysed to release their human leucocyte elastase which hydrolyzes a chromogenic ester to provide a phenol which then reacts with a diazonium salt to form a colored azo dye.
摘要翻译：本发明涉及用于测定流体测试样品中的酶活性或酶底物浓度的组合物和方法，其中酶催化试剂系统中的反应以提供指示酶或酶底物浓度的可检测响应测试样品。测定量的能够消除酶活性的蛋白酶被包含在试剂组合物中，使得当测量的测试样品被处理时，产生可检测反应的反应将具有确定的终点。本发明特别适用于尿中白细胞的测定，其中白细胞被裂解以释放其人类白细胞弹性蛋白酶，其水解显色酯以提供酚，然后与重氮盐反应形成着色偶氮染料。

65. 发明授权

US5424215A Assay for the determination of protein in a biological sample 失效
标题翻译：测定生物样品中蛋白质的测定
公开(公告)号：US5424215A
公开(公告)日：1995-06-13
申请号：US192345
申请日：1994-02-07
申请人： James P. Albarella , Angela A. Michaels , Michael J. Pugia , Ronald G. Sommer
发明人： James P. Albarella , Angela A. Michaels , Michael J. Pugia , Ronald G. Sommer
IPC分类号： G01N33/493 , G01N33/52 , G01N33/68 , G01N33/00
CPC分类号： G01N33/6839 , G01N33/52
摘要： The detection of protein is accomplished using novel composition and method involving phenolsulfonephthalein protein error indicator, buffer and an aliphatic ether-polycarbonate present in an amount equal to or less than ten percent by weight.
摘要翻译：使用涉及酚磺酞蛋白错误指示剂，缓冲剂和等于或小于10重量％的脂族醚 - 聚碳酸酯的新型组合物和方法来实现蛋白质的检测。

66. 发明授权

US5399498A Reduction of background interferences in the molybdate-dye protein assay 失效
标题翻译：降低钼酸盐染料蛋白质测定中的背景干扰
公开(公告)号：US5399498A
公开(公告)日：1995-03-21
申请号：US168220
申请日：1993-12-17
申请人： Michael J. Pugia
发明人： Michael J. Pugia
IPC分类号： G01N31/22 , G01N33/68 , G01N33/00
CPC分类号： G01N33/6839 , G01N33/683 , Y10T436/105831
摘要： Disclosed is an improvement to the assay for protein in urine involving the use of a molybdate or tungstate salt and an indicator dye which forms a complex with molybdate or tungstate whose absorption band is shifted in the presence of protein. The improvement involves the use of an ionizable phosphate containing compound characterized by the formula: ##STR1## to reduce background interference caused by constituents normally present in urine.
摘要翻译：本发明涉及使用钼酸盐或钨酸盐和与钼酸盐或钨酸盐形成络合物的指示剂染料在尿液中的蛋白质测定的改进，其吸收带在蛋白质存在下移动。改进之处在于使用以下化学式表征的可电离磷酸盐化合物：以减少通常存在于尿液中的成分引起的背景干扰。

67. 发明授权

US5106753A Method for determining manganese level in blood using a porphyrin composition 失效
标题翻译：使用卟啉组合物测定血液中锰含量的方法
公开(公告)号：US5106753A
公开(公告)日：1992-04-21
申请号：US468665
申请日：1990-01-23
申请人： Marvin A. Genshaw , Michael J. Pugia
发明人： Marvin A. Genshaw , Michael J. Pugia
IPC分类号： G01N33/50 , C12Q1/26 , G01N1/28 , G01N33/52 , G01N33/84
CPC分类号： G01N33/84 , C12Q1/26 , G01N33/523 , G01N2800/04
摘要： The present invention is directed to a simple and inexpensive liquid or dry reagent assay system for detecting nanomolar amounts of manganese in body fluids in microgram per liter concentrations. More specifically, the manganese assay of this invention is directed to the optical detection of a chromogenic reaction, such as the oxidation of a redox indicator, catalyzed by a manganese porphyrin complex.
摘要翻译：本发明涉及一种用于以微克/升浓度检测体液中的纳摩尔量的锰的简单廉价的液体或干试剂测定系统。更具体地，本发明的锰测定涉及由锰卟啉络合物催化的显色反应的光学检测，例如氧化还原指示剂的氧化。

68. 发明授权

US5096827A Composition for preventing unwanted oxidation of redox indicators 失效
标题翻译：用于防止氧化还原指示剂的不希望的氧化的组合物
公开(公告)号：US5096827A
公开(公告)日：1992-03-17
申请号：US284685
申请日：1988-12-15
申请人： Michael J. Pugia
发明人： Michael J. Pugia
IPC分类号： C12Q1/26 , G01N31/22
CPC分类号： G01N31/22 , C12Q1/26 , Y10T436/10 , Y10T436/108331
摘要： The present invention is directed to a reliable redox indicator composition for use in chemical assay systems and is particularly well suited for assay systems using a dry phase format. More specifically, the improved indicator composition of this invention is directed to the use of a divalent metal complex to prevent unwanted metal hydroperoxide mediated and similar-type oxidation of redox indicators. A fundamental element of the present invention involves the use of divalent metal complexes of the general formula: ##STR1## wherein M is either Zn(II), Co(II), Mn(II), or Rb(II).
摘要翻译：本发明涉及用于化学测定系统的可靠的氧化还原指示剂组合物，并且特别适用于使用干相形式的测定系统。更具体地，本发明的改进的指示剂组合物涉及使用二价金属络合物来防止不需要的金属氢过氧化物介导的氧化还原指示剂的类似氧化。本发明的基本要素涉及使用通式如下的二价金属络合物：其中M为Zn（II），Co（II），Mn（II）或Rb（II）。

69. 发明授权

US5089420A Composition, device and method of assaying for a peroxidatively active substance utilizing amine borate compounds 失效
标题翻译：组合物，装置和使用氨基硼酸盐化合物的过氧化活性物质的测定方法
公开(公告)号：US5089420A
公开(公告)日：1992-02-18
申请号：US472282
申请日：1990-01-30
申请人： James P. Albarella , Michael J. Pugia
发明人： James P. Albarella , Michael J. Pugia
IPC分类号： G01N31/00 , C12Q1/28 , G01N31/22 , G01N33/50 , G01N33/52 , G01N33/72
CPC分类号： C12Q1/28 , G01N33/725 , C12Q2326/12 , Y10S435/805 , Y10S436/904 , Y10T436/25125
摘要： A new and improved test device and method of determining the presence or concentration of a peroxidatively active substance, such as hemoglobin, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with a perioxidatively active substance to produce a detectable or measurable response. In addition, a new and improved indicator reagent composition, comprising an indicator dye, such as a redox indicator, like a benzidine indicator; a hydroperoxide; an amine borate compound having the general structural formula: ##STR1## wherein R.sub.1, R.sub.2, and R.sub.3 are, independently, methyl groups or ethyl groups, and m, n and p are numerals ranging from one to about three; a buffer, is incorporated into a suitable carrier matrix to provide a more accurate and trustworthy assay of a test sample for a peroxidatively active substance. The improved method and composition are especially useful in the assay of urine for occult blood.

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