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    • 63. 发明申请
    • Focused acoustic ejection cell sorting system and method
    • 聚焦声弹出细胞分选系统及方法
    • US20050130257A1
    • 2005-06-16
    • US11049206
    • 2005-02-01
    • Mitchell MutzRichard EllsonDavid Lee
    • Mitchell MutzRichard EllsonDavid Lee
    • B41J2/14C12M1/26C12M3/00C12N5/02G01N15/10G01N15/14G01N30/02C12P33/02B07C5/02
    • G01N35/1074B41J2/14008C12M47/04G01N15/1056G01N29/0681G01N30/02G01N2015/1081G01N2015/1415G01N2015/142G01N2035/1039B01D2015/389
    • A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.
    • 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴,每个流体液滴包含一个或多个颗粒或其它局部体积。 可以是活细胞的局部体积被排出到基底表面,容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统,其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列,其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法,其比单体阵列方法更有效,快速,灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效,连续和同时的分选,并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统,用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测,选择和排出所选择的颗粒或外切体积(例如活细胞) 。
    • 64. 发明申请
    • Air purification system and method
    • 空气净化系统及方法
    • US20050109209A1
    • 2005-05-26
    • US10862364
    • 2004-06-08
    • David Lee
    • David Lee
    • B01D45/12B01D53/14B01D53/26F24F3/14F24F3/16
    • B01D53/14B01D45/12B01D53/265F24F3/1405F24F3/16F24F2003/1617F24F2006/146Y02A50/21
    • The air purification system and method for purifying and cooling air for use in a building has an air pollutant removal stage, an air pre-cooling unit, and a dual cooling and water removal unit, including a cyclone separator having a cooling coil wrapped around the separator for condensing and removing water from the airflow. The method comprises the steps of removing pollutants from an incoming polluted airflow to provide a substantially pollutant free outgoing moist airflow; pre-cooling the substantially pollutant free moist airflow to provide a clean cool moist airflow; and condensing water out of the clean cool moist airflow to provide a substantially clean dry airflow suitable for use in a building.
    • 用于建筑物中的净化和冷却空气的空气净化系统和方法具有空气污染物去除阶段,空气预冷单元和双重冷却和除水单元,包括旋风分离器,该旋风分离器具有围绕 用于冷凝和从气流中除去水分离器。 该方法包括从进入的污染气流中除去污染物以提供基本上无污染的流出湿气流的步骤; 预冷却基本上无污染的潮湿气流,提供清洁的潮湿气流; 并将水从干净的潮湿气流中冷凝出来,提供适合用于建筑物的基本干净的干燥气流。