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61. 发明授权

US5804103A Di-substituted 1,2-dioxetane compounds having increased water solubility and assay compositions 失效
标题翻译：具有增加的水溶性和测定组成的二取代的1,2-二氧杂环丁烷化合物
公开(公告)号：US5804103A
公开(公告)日：1998-09-08
申请号：US842728
申请日：1997-04-16
申请人： Hashem Akhavan-Tafti , Renuka DeSilva , Arthur Paul Schaap
发明人： Hashem Akhavan-Tafti , Renuka DeSilva , Arthur Paul Schaap
IPC分类号： G01N33/535 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N33/53 , G01N33/543 , G01N33/58 , C09K3/00
CPC分类号： G01N33/582 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , C07C2103/00
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. Compositions comprising a dioxetane with two or more water-solubilizing groups, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译：提供了具有改善的水溶性的稳定的酶触发的化学发光的1,2-二氧杂环丁烷。进一步被二氧杂环丁烷结构上的两个或更多个水溶性基团取代的二氧杂环丁烷通过消除当用于胶囊化学分析系统的分析中时试剂残留的问题而提供优异的性能。还提供了包含二环氧乙烷与两个或多个水溶性基团的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。

62. 发明授权

US5750698A Aryl N-alkylacridancarboxylate derivatives useful for chemiluminescent detection 失效
标题翻译：用于化学发光检测的N-烷基吖啶甲酸N-芳基酯衍生物
公开(公告)号：US5750698A
公开(公告)日：1998-05-12
申请号：US602464
申请日：1996-02-16
申请人： Hashem Akhavan-Tafti , Renuka DeSilva , Zahra Arghavani , Barry A. Schoenfelner
发明人： Hashem Akhavan-Tafti , Renuka DeSilva , Zahra Arghavani , Barry A. Schoenfelner
IPC分类号： G01N33/532 , C07D219/02 , C07D219/04 , C07D219/06 , C09K11/07 , C12Q1/28 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/533 , G01N33/535 , G01N33/566
CPC分类号： G01N33/533 , C07D219/02 , C07D219/04 , C07D219/06 , C12Q1/28 , Y10S435/968
摘要： Acridans which are reactable with a peroxidase and peroxide. The acridans are characterized by having an aromatic leaving group ArO which is a di- or polyhalosubstituted phenoxy group. The compounds are useful in assays where one member of a binding pair is linked to the peroxidase and for detecting the peroxidase. The method can also be used to detect hydrogen peroxide.
摘要翻译：可与过氧化物酶和过氧化物反应的吖啶。吖啶的特征在于具有芳族离去基团ArO，其为二取代或多取代的苯氧基。该化合物可用于其中一个结合对成员与过氧化物酶连接并检测过氧化物酶的测定中。该方法也可用于检测过氧化氢。

63. 发明授权

US5723295A Methods, acridan compounds and kits for producing light 失效
标题翻译：方法，吖啶化合物和用于产生光的试剂盒
公开(公告)号：US5723295A
公开(公告)日：1998-03-03
申请号：US644088
申请日：1996-05-09
申请人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
发明人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
IPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C12Q1/28 , G01N33/535
CPC分类号： C07D219/06 , C07D219/02 , C07D219/04 , C12Q1/28 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assay method, compositions, kits and chemiluminescent acridan compounds are described which use a two-step chemiluminescent reaction process. The reaction involves an acridan compound, preferably a derivative of an N-alkylacridan-9-carboxylic acid, which undergoes a reaction with a peroxide compound, a peroxidase enzyme and an enhancer under conditions of time, temperature and pH which permit the accumulation of an intermediate compound, which is subsequently induced to produce a burst of light by raising the pH. The result is generation of very high intensity light from the reaction. The peroxidase enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule. The method is particularly amenable to automated assays because of the separation of the incubation and light generating steps.
摘要翻译：描述了使用两步化学发光反应过程的化学发光测定方法，组合物，试剂盒和化学发光的吖啶化合物。该反应涉及吖啶化合物，优选N-烷基吖啶-9-羧酸的衍生物，其在时间，温度和pH条件下与过氧化物，过氧化物酶和增强剂发生反应，这允许积聚中间体化合物，其随后通过提高pH而被诱导产生突发性的光。结果是从反应产生非常高强度的光。过氧化物酶在免疫测定，DNA探针测定或其他测定中单独存在或连接到特异性结合对的成员，其中水解酶与报道分子结合。由于孵育和光生成步骤的分离，该方法特别适用于自动化测定。

64. 发明授权

US5652345A 1,2-dioxetane compound 失效
标题翻译： 1,2-二氧环乙烷化合物
公开(公告)号：US5652345A
公开(公告)日：1997-07-29
申请号：US186461
申请日：1994-01-25
申请人： Arthur Paul Schaap , Hashem Akhavan-Tafti
发明人： Arthur Paul Schaap , Hashem Akhavan-Tafti
IPC分类号： G01N21/78 , C07C37/00 , C07C37/62 , C07C39/19 , C07C39/38 , C07C43/178 , C07C51/15 , C07C69/14 , C07C69/24 , C07C69/76 , C07C305/04 , C07D207/46 , C07D277/68 , C07D311/80 , C07D311/82 , C07D311/86 , C07D321/00 , C07D405/12 , C07D407/10 , C07D407/12 , C07D417/12 , C07D493/10 , C07D495/04 , C07F7/18 , C07F9/12 , C07F9/6541 , C07F9/655 , C07F9/6558 , C07F9/6561 , C07H15/203 , C07H15/26 , C09K11/07 , C12Q1/44 , G01N31/22 , C07H3/00
CPC分类号： C07D405/12 , C07C43/1788 , C07C51/15 , C07D207/46 , C07D311/82 , C07D321/00 , C07D407/10 , C07D407/12 , C07D417/12 , C07D493/10 , C07D495/04 , C07F7/1852 , C07F7/1856 , C07F9/6541 , C07F9/65512 , C07F9/65522 , C07F9/65586 , C07F9/6561 , C07H15/203 , C07C2103/18 , C07C2103/74
摘要： 4-(3-beta-D-galactopyranosylphenyl)-4-methoxyspiro[1,2-dioxetane-3,2'-adamantane] which is useful in various chemiluminescent assays.
摘要翻译： 4-（3-β-D-吡喃半乳糖基苯基）-4-甲氧基螺[1,2-二氧杂环丁烷-2,2'-金刚烷]，其可用于各种化学发光测定。

65. 发明授权

US5593845A Aryl N-alkylacridancarboxylate derivatives useful for chemiluminescent detection 失效
标题翻译：用于化学发光检测的N-烷基吖啶甲酸N-芳基酯衍生物
公开(公告)号：US5593845A
公开(公告)日：1997-01-14
申请号：US205093
申请日：1994-03-02
申请人： Hashem Akhavan-Tafti , Renuka DeSilva , Zahra Arghavani , Barry A. Schoenfelner
发明人： Hashem Akhavan-Tafti , Renuka DeSilva , Zahra Arghavani , Barry A. Schoenfelner
IPC分类号： G01N33/532 , C07D219/02 , C07D219/04 , C07D219/06 , C09K11/07 , C12Q1/28 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/533 , G01N33/535 , G01N33/566
CPC分类号： G01N33/533 , C07D219/02 , C07D219/04 , C07D219/06 , C12Q1/28 , Y10S435/968
摘要： Acridans which are reactable with a peroxidase and peroxide. The acridans are characterized by having an aromatic leaving group ArO which is a di- or polyhalosubstituted phenoxy group. The compounds are useful in assays where one member of a binding pair is linked to the peroxidase and for detecting the peroxidase. The method can also be used to detect hydrogen peroxide.
摘要翻译：可与过氧化物酶和过氧化物反应的吖啶。吖啶的特征在于具有芳族离去基团ArO，其为二取代或多取代的苯氧基。该化合物可用于其中一个结合对成员与过氧化物酶连接并检测过氧化物酶的测定中。该方法也可用于检测过氧化氢。

66. 发明授权

US5578253A Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use 失效
标题翻译：化学发光二烷基取代的1,2-二氧杂环丁烷化合物，合成和使用方法
公开(公告)号：US5578253A
公开(公告)日：1996-11-26
申请号：US344124
申请日：1994-11-23
申请人： Arthur P. Schaap , Hashem Akhavan-Tafti
发明人： Arthur P. Schaap , Hashem Akhavan-Tafti
IPC分类号： G01N21/78 , C07C43/178 , C07C69/017 , C07C69/12 , C07C69/22 , C07C69/78 , C07D321/00 , C07F7/18 , C07F9/12 , C07F9/655 , C09K11/07 , C12Q1/34 , G01N33/533 , G01N33/58 , C09K3/00
CPC分类号： G01N33/582 , C07C43/1782 , C07C43/1787 , C07C69/017 , C07D321/00 , C07F7/1852 , C07F9/12 , C07F9/6551 , C07F9/65512 , C12Q1/34 , G01N33/533 , C07C2101/14
摘要： A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.
摘要翻译：描述了化学发光测定方法和组合物，其使用被去保护以触发化学发光反应的二烷基取代的二氧杂环丁烷。公开了在二氧环乙烷环上取代的化学发光的1,2-二氧杂环丁烷化合物，其具有可由试剂触发以产生光的两个非标记烷基。二烷基取代的二氧杂环丁烷可用于检测包括酶在内的引发剂。该酶可以在免疫测定，DNA探针测定或其它酶结合报告分子的其它测定中单独存在或连接到特异性结合对的成员。

67. 发明授权

US5439617A Polymeric phosphonium salts providing enhanced chemiluminescence from 1,2-dioxetanes 失效
公开(公告)号：US5439617A
公开(公告)日：1995-08-08
申请号：US195100
申请日：1994-02-14
申请人： Hashem Akhavan-Tafti
发明人： Hashem Akhavan-Tafti
IPC分类号： C09K11/07 , C08F8/40 , C08F30/02 , C08F230/02 , C08K5/156 , C08L43/00 , C08L43/02 , G01N21/76 , G01N33/533 , G01N33/58 , C09K3/00
CPC分类号： G01N33/582 , G01N21/76 , G01N33/533
摘要： Polymeric phosphonium salts useful for enhancing the chemiluminescence produced by 1,2-dioxetanes are described. The polymers are preferably water soluble and used in immunoassays and nucleic acid assays with enzyme triggerable 1,2-dioxetanes.

68. 发明申请

US20130084652A1 Homogeneous Chemiluminescence Assay Methods with Increased Sensitivity 审中-公开
标题翻译：均相化学发光测定方法增加灵敏度
公开(公告)号：US20130084652A1
公开(公告)日：2013-04-04
申请号：US13641476
申请日：2011-05-13
申请人： Nir Shapir , Michael Salvati , Jeff Todtleben , Hashem Akhavan-Tafti , Richard S. Handley
发明人： Nir Shapir , Michael Salvati , Jeff Todtleben , Hashem Akhavan-Tafti , Richard S. Handley
IPC分类号： G01N21/76
CPC分类号： G01N21/76 , C12Q1/28 , C12Q1/66 , G01N33/5306 , G01N33/582
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. Non-specific signal generated by singlet oxygen not in proximity is reduced or suppressed using a singlet oxygen quencher (SOQ). The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Use of Noise Modulation Agents significantly improves signal-to-noise ratios and assay sensitivity. Compositions and kits are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中的分析物的方法。一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。使用单线态氧猝灭剂（SOQ）降低或抑制不接近的单线态氧产生的非特异性信号。活化的化学发光化合物随后产生光。所产生的光量与介质中分析物的量有关。使用噪声调制剂可显着提高信噪比和测定灵敏度。还公开了组合物和试剂盒。

69. 发明申请

US20120094313A1 Nonseparation Assay Methods 有权
标题翻译：非分离测定方法
公开(公告)号：US20120094313A1
公开(公告)日：2012-04-19
申请号：US13315834
申请日：2011-12-09
申请人： Hashem Akhavan-Tafti
发明人： Hashem Akhavan-Tafti
IPC分类号： G01N33/566 , C12M1/34
CPC分类号： G01N33/54306 , C12Q1/28
摘要： Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays.
摘要翻译：披露了与已知方法相比简化的特异性结合反应的测定方法。能够产生化学发光的化合物固定在固体支持物上，就像用于从样品中捕获分析物的特异性结合对的成员一样。激活化学发光化合物并与特异性结合对成员结合的活化剂化合物与样品一起被加入到固体支持物中。触发溶液的添加在活化剂结合物被特异结合的位点处引起化学发光反应。测定方法称为非分离测定法，因为它们在检测步骤之前不需要除去或分离过量检测标记（活化剂缀合物）。该方法适用于各种类型的测定，包括免疫测定，受体 - 配体测定和核酸杂交测定。

70. 发明授权

US08137990B2 Compositions for chemiluminescent detection of hydrogen peroxide 有权
标题翻译：化学发光检测过氧化氢的组合物
公开(公告)号：US08137990B2
公开(公告)日：2012-03-20
申请号：US12541245
申请日：2009-08-14
申请人： Hashem Akhavan-Tafti , Robert A. Eickholt , Kenneth S. Lauwers , Richard S. Handley
发明人： Hashem Akhavan-Tafti , Robert A. Eickholt , Kenneth S. Lauwers , Richard S. Handley
IPC分类号： G01N33/533 , G01N21/76 , C07D321/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译：公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。

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