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    • 66. 发明申请
    • Estimation method of fluorescent dye's concentration from multiple fluorescence and the estimation method of fluorescent intensity from multiple fluorescence
    • 多重荧光荧光染料浓度的估计方法和多重荧光荧光强度的估算方法
    • US20060200318A1
    • 2006-09-07
    • US11218466
    • 2005-09-06
    • So-ichiro MorishitaHideo YokotaRyutaro HimenoTaketoshi Mishima
    • So-ichiro MorishitaHideo YokotaRyutaro HimenoTaketoshi Mishima
    • G06F19/00G01N31/00
    • G01N21/6428G01N2201/1293
    • It is an object of the invention to provide an estimation method of fluorescent dye's concentration from multiple fluorescence, where the accurate estimation of the fluorescent dye's concentration of each fluorescent dye from multiple fluorescence is made possible and the separation of multiple fluorescence which is difficult in the prior art is made possible. In the estimation method of fluorescent dye's concentration from multiple fluorescence where the fluorescent dye's concentration from measured multiple fluorescence, independent component analysis is performed to the spectrum of fluorescent dye where fluorescent dye's concentration is known to derive the intensity of an independent component, regression analysis is performed by using the derived intensity of the independent component as a variable to estimate the fluorescent dye's concentration function of the fluorescent dye where the fluorescent dye's concentration is known, and the concentration of fluorescent dye is estimated from the measured multiple fluorescence based on the estimated fluorescent dye's concentration function.
    • 本发明的一个目的是提供一种荧光染料从多个荧光的浓度的估计方法,其中可以准确地估计荧光染料从多个荧光的荧光染料的浓度,并且在多个荧光中难以分离多个荧光 现有技术成为可能。 在荧光染料浓度从荧光染料浓度测量多个荧光的荧光染料浓度的估计方法中,对已知荧光染料浓度的荧光染料的光谱进行独立成分分析,得出独立成分的强度,回归分析为 通过使用独立成分的衍生强度作为变量来进行荧光染料的荧光染料的浓度函数的荧光染料的浓度的测定,荧光染料的浓度是已知的,荧光染料的浓度是根据测定的多个荧光估计的 染料的浓度功能。