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61. 发明授权

US07833994B2 Ethanol extraction of phytosterols from corn fiber 有权
标题翻译：乙醇从玉米纤维中提取植物甾醇
公开(公告)号：US07833994B2
公开(公告)日：2010-11-16
申请号：US11084933
申请日：2005-03-21
申请人： Charles Abbas , Kyle E. Beery , Thomas P. Binder , Anne M. Rammelsberg
发明人： Charles Abbas , Kyle E. Beery , Thomas P. Binder , Anne M. Rammelsberg
IPC分类号： A61K31/56 , A61K31/575
CPC分类号： A23L33/11 , A23V2002/00 , A23V2250/2136
摘要： The present invention provides a process for extracting sterols from a high solids, thermochemically hydrolyzed corn fiber using ethanol as the extractant. The process includes obtaining a corn fiber slurry having a moisture content from about 20 weight percent to about 50 weight percent solids (high solids content), thermochemically processing the corn fiber slurry having high solids content of 20 to 50% to produce a hydrolyzed corn fiber slurry, dewatering the hydrolyzed corn fiber slurry to achieve a residual corn fiber having a moisture content from about 30 to 80 weight percent solids, washing the residual corn fiber, dewatering the washed, hydrolyzed corn fiber slurry to achieve a residual corn fiber having a moisture content from about 30 to 80 weight percent solids, and extracting the residual corn fiber with ethanol and separating at least one sterol.
摘要翻译：本发明提供使用乙醇作为萃取剂从高固体，热化学水解的玉米纤维中提取固醇的方法。该方法包括获得具有约20重量％至约50重量％固体（高固体含量）的水分含量的玉米纤维浆料，热化学处理具有20至50％的高固含量的玉米纤维浆料以产生水解玉米纤维将水解的玉米纤维浆液脱水，以获得含水量为约30至80重量％固体的残余玉米纤维，洗涤残余的玉米纤维，对经洗涤的水解玉米纤维浆液进行脱水以获得具有水分的残余玉米纤维含量为约30至80重量％的固体，并用乙醇萃取残余的玉米纤维并分离至少一种甾醇。

62. 发明授权

US07723097B2 Escherichia coli strains that over-produce L-threonine and processes for their production 有权
标题翻译：产生L-苏氨酸的大肠杆菌菌株及其生产过程
公开(公告)号：US07723097B2
公开(公告)日：2010-05-25
申请号：US11371732
申请日：2006-03-09
申请人： John N. D'Elia , Sean W. Jordan
发明人： John N. D'Elia , Sean W. Jordan
IPC分类号： C12N15/53 , C12N15/63 , C12N1/21
CPC分类号： C12P13/08 , C12N9/0008 , C12P13/06 , C12P13/12
摘要： The invention relates to novel bacterial strains and constructs as well as methods for production of L-amino acids, including but not limited to L-threonine. Such novel bacterial strains may be characterized by, for instance, Escherichia coli strains in which an aspartate semialdehyde dehydrogenase (asd) gene is operably associated with at least one non-native promoter, non-native ribosome binding site, or both.
摘要翻译：本发明涉及新的细菌菌株和构建体以及生产L-氨基酸的方法，包括但不限于L-苏氨酸。这样的新型细菌菌株的特征可以是例如其中天冬氨酸半醛脱氢酶（asd）基因与至少一种非天然启动子，非天然核糖体结合位点或两者可操作地相关的大肠杆菌菌株。

63. 发明申请

US20100112651A1 COMPOSITIONS AND METHODS FOR MANIPULATING CARBON FLUX IN CELLS 有权
标题翻译：用于在细胞中操作碳通量的组合物和方法
公开(公告)号：US20100112651A1
公开(公告)日：2010-05-06
申请号：US12409072
申请日：2009-03-23
申请人： Beth Fatland-Bloom , P. John Rayapati , Nyerhovwo John Tonukari
发明人： Beth Fatland-Bloom , P. John Rayapati , Nyerhovwo John Tonukari
IPC分类号： C12P7/56 , C07H21/00 , C07H21/02 , C12N15/74 , C12N1/21 , C12N1/19 , C12N1/15 , C12P7/46 , C12P7/20
CPC分类号： C12P7/46 , C12N9/93 , C12P7/20 , C12P7/56 , C12Y604/01001
摘要： Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.
摘要翻译：提供编码通过代谢途径改变碳通量的酶的核苷酸和蛋白质序列，其导致宿主细胞如米曲霉细胞中的乳酸或富马酸盐的产生。还提供了操作细胞中碳通量的方法。

64. 发明授权

US07687253B2 Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation 有权
公开(公告)号：US07687253B2
公开(公告)日：2010-03-30
申请号：US12051064
申请日：2008-03-19
申请人： Hungming J. Liaw , Jill S. Bradshaw , Yueqin Yang , Weiying Mao
发明人： Hungming J. Liaw , Jill S. Bradshaw , Yueqin Yang , Weiying Mao
IPC分类号： C12N1/20 , C12N9/00 , C12N9/02 , C12N15/01 , C07H21/02
CPC分类号： C12P13/08 , C12N15/01 , C12N15/52 , C12R1/19
摘要： The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.

65. 发明授权

US07667061B2 Method of preparing a composition using argentation chromatography 有权
标题翻译：使用梯度色谱法制备组合物的方法
公开(公告)号：US07667061B2
公开(公告)日：2010-02-23
申请号：US11612250
申请日：2006-12-18
申请人： Thomas P. Binder , Doug Geier , Ahmad K. Hilaly , Robert Duane Sandage , John G. Soper
发明人： Thomas P. Binder , Doug Geier , Ahmad K. Hilaly , Robert Duane Sandage , John G. Soper
IPC分类号： C11B7/00 , B01D15/00
CPC分类号： B01D15/1828 , B01D15/20 , B01D15/362 , B01J20/0233 , B01J20/284 , B01J20/3234 , B01J20/3236 , B01J47/016 , C07C67/56 , C11B3/10 , G01N30/462 , C07C69/587 , C07C69/58
摘要： The present invention is directed to a method of preparing compositions enriched in compounds containing carbon chains of varying degrees of unsaturation using argentation chromatography. The present method utilizes an argentized cationic resin or a conditioned argentized alumina to separate compounds containing saturated or mono-unsaturated carbon chains from compounds having polyunsaturated carbon chains present in a starting composition. The invention is particularly useful for preparing a composition enriched in polyunsaturated fatty acid alkyl esters from mixtures of fatty acid esters in a starting composition derived from vegetable oils. The present invention is also directed to a method of preparing a conditioned argentized alumina adsorbent having increased selectivity for compounds containing one or more polyunsaturated carbon chains.
摘要翻译：本发明涉及使用梯度色谱法制备富集含有不同不饱和度的碳链的化合物的组合物的方法。本方法利用阳离子阳离子树脂或调节的氧化铝将起始组合物中存在的具有多不饱和碳链的化合物与饱和或单不饱和碳链分开。本发明特别可用于在源自植物油的起始组合物中由脂肪酸酯的混合物制备富含多不饱和脂肪酸烷基酯的组合物。本发明还涉及一种制备具有对含有一个或多个多不饱和碳链的化合物具有增加的选择性的调节的氧化铝吸附剂的方法。

66. 发明授权

US07635579B2 Metabolic engineering of amino acid production 有权
标题翻译：氨基酸生产代谢工程
公开(公告)号：US07635579B2
公开(公告)日：2009-12-22
申请号：US11978908
申请日：2007-10-30
申请人： P. John Rayapati , Corey M. Crafton
发明人： P. John Rayapati , Corey M. Crafton
IPC分类号： C12P13/04 , C12P13/08 , C12N15/74 , C12N1/20
CPC分类号： C12N15/01 , C12N9/88 , C12P13/04 , C12P13/08
摘要： The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be quantified by the formula: [(g amino acid produced/g dextrose consumed)*100]=% Yield and expressed as yield from dextrose. The invention provides microorganisms that are made auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids by mutagenesis and selected for their ability to produce higher percent yields of the desired amino acid than the parental strain. Preferred microorganisms are Corynebacterium, Brevibacterium or Escherichia coli producing L-lysine. Mutagenesis is performed by classical techniques or through rDNA methodology. Methods of the invention are designed to increase the production of an amino acid by mutagenizing a parental strain, selecting cells auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids and selecting branched chain amino acid auxotrophs or bradytrophs that produce a higher percent yield from dextrose of the desired amino acid than the parental strain. Processes of the invention are designed for the production an amino acid comprising culturing in a medium a microorganism obtained by mutagenizing a parent strain to be auxotrophic or bradytrophic for branched chain amino acid synthesis and selecting variants that are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain.
摘要翻译：本发明涉及氨基酸的发酵生产，提供微生物，对其有用的方法和方法。本发明的微生物能够以比亲本菌株更高的效率将葡萄糖转化成L-异亮氨酸，L-亮氨酸和L-缬氨酸以外的氨基酸。转化效率可以通过以下公式进行定量：[（g氨基酸产生/ g葡萄糖消耗）* 100] =％产率并以葡萄糖的收率表示。本发明提供了通过诱变合成一种或多种支链氨基酸产生营养缺陷型或营养缺陷型微生物，并且选择其产生比亲本菌株更高产率的所需氨基酸的能力。优选的微生物是产生L-赖氨酸的棒状杆菌，短杆菌或大肠杆菌。诱变通过经典技术或通过rDNA方法进行。本发明的方法被设计成通过诱变亲本菌株来增加氨基酸的产生，选择营养缺陷型或营养型细胞用于合成一种或多种支链氨基酸并选择产生更高百分比的支链氨基酸营养缺陷型或营养缺陷型来自所需氨基酸的葡萄糖的产量高于亲本菌株。本发明的方法被设计用于生产氨基酸，其包括在培养基中培养通过将亲本菌株诱变为营养缺陷型或营养型的支链氨基酸合成获得的微生物，并选择能够将葡萄糖转化成除了氨基酸以外的氨基酸的变体 L-异亮氨酸，L-亮氨酸和L-缬氨酸，其效力高于亲本菌株。

67. 发明授权

US07582777B2 Long chain (C22-C50) polyunsaturated hydrocarbons, derivatives, synthesis and uses thereof 有权
标题翻译：长链（C22-C50）多不饱和烃，衍生物，合成及用途
公开(公告)号：US07582777B2
公开(公告)日：2009-09-01
申请号：US11250622
申请日：2005-10-17
申请人： Paul D. Bloom
发明人： Paul D. Bloom
IPC分类号： C07D303/04 , C07D319/10 , C07D407/06 , C07D407/14 , C07C1/20
CPC分类号： C07D319/12 , C08G59/226 , C08G59/36 , C08G59/38
摘要： The present invention is directed to unsaturated or derivatized long chain (C22-C50) polyunsaturated hydrocarbons and a method of preparing the long chain hydrocarbons via electrocoupling of C12-C26 fatty acids. It has been found that soapstock is an inexpensive source of starting materials for the present method. The present invention is also directed to compositions comprising the long chain polyunsaturated hydrocarbons, which can be used as reactive diluents and modifiers in latex, epoxy, alkyd and polymer compositions. In another aspect, the present invention is directed to derivitization or ozonolysis of the long chain polyunsaturated hydrocarbons. The present method is also useful for preparing C12-26 alkyl esters and C12-26 carbon chain compounds containing a terminal olefin.
摘要翻译：本发明涉及不饱和或衍生的长链（C22-C50）多不饱和烃和通过C 12 -C 26脂肪酸的电偶合制备长链烃的方法。已经发现，皂片是本方法的起始材料的廉价源。本发明还涉及包含长链多不饱和烃的组合物，其可以用作胶乳，环氧树脂，醇酸树脂和聚合物组合物中的反应性稀释剂和改性剂。另一方面，本发明涉及长链多不饱和烃的衍生或臭氧分解。本发明方法也可用于制备含有末端烯烃的C12-26烷基酯和C12-26碳链化合物。

68. 发明申请

US20090178928A1 Process for Desalting Glycerol Solutions and Recovery of Chemicals 审中-公开
标题翻译：脱盐甘油溶液和化学品回收工艺
公开(公告)号：US20090178928A1
公开(公告)日：2009-07-16
申请号：US12165384
申请日：2008-06-30
申请人： Jeremie Ray GROOS , Ahmad K. Hilaly , Krishnamurthy Mani , Rishi Shukla
发明人： Jeremie Ray GROOS , Ahmad K. Hilaly , Krishnamurthy Mani , Rishi Shukla
IPC分类号： B01D61/44 , B01D61/46
CPC分类号： C11C1/08 , B01D61/42 , B01D61/445 , B01D61/58 , C07C29/76 , C07C67/03 , C10G2300/1011 , C11C3/003 , Y02E50/13 , Y02P30/20 , C07C31/225 , C07C69/52 , C07C69/24
摘要： Processes for desalting glycerol-rich solutions or process streams using electrodialysis are provided. The glycerol-rich process streams are typically byproducts from the production of biodiesel. Following electrodialysis, the resulting aqueous salt solution is placed in a water splitting cell to recover the acid and base components of the salt. These acid and base components, in turn, can be reused in other processes, such as biodiesel production.
摘要翻译：提供了使用电渗析脱盐富含甘油的溶液或工艺流的方法。富含甘油的工艺流通常是来自生物柴油生产的副产物。电渗析后，将所得的盐水溶液置于水分解池中以回收盐的酸和碱组分。这些酸和碱组分又可以用于其他工艺，例如生物柴油生产。

69. 发明授权

US07524526B2 Process for producing high purity isoflavones 失效
标题翻译：生产高纯度异黄酮的方法
公开(公告)号：US07524526B2
公开(公告)日：2009-04-28
申请号：US10409683
申请日：2003-04-09
申请人： Ahmad K. Hilaly , Bob Sandage , John Soper
发明人： Ahmad K. Hilaly , Bob Sandage , John Soper
IPC分类号： A23J1/00
CPC分类号： C07D311/36
摘要： A process for the production of a high purity isoflavone composition is disclosed. The process involves subjecting plant material to a primary chromatographic step to obtain an isoflavone enriched fraction and subjecting the isoflavone enriched fraction to a secondary chromatographic step. Also disclosed is a process that involves passing the plant material through an ultrafiltration membrane which has a molecular weight cut-off range that produces a plant material permeate prior to subjecting the plant material permeate to primary and secondary chromatographic steps.
摘要翻译：公开了一种生产高纯度异黄酮组合物的方法。该方法包括使植物材料进行初级色谱步骤以获得富含异黄酮的级分并使富含异黄酮的级分进行二级色谱步骤。还公开了一种方法，其涉及使植物材料通过超滤膜，该超滤膜具有在使植物材料渗透进入初级和次级色谱步骤之前产生植物材料渗透物的分子量截留范围。

70. 发明申请

US20090094079A1 EVALUATING COMMODITY CONDITIONS USING AERIAL IMAGE DATA 有权
标题翻译：使用空中影像数据评估商品条件
公开(公告)号：US20090094079A1
公开(公告)日：2009-04-09
申请号：US12248110
申请日：2008-10-09
申请人： Charles Linville
发明人： Charles Linville
IPC分类号： G06Q30/00 , G01C21/20 , G06K9/46 , H04N7/18 , G06Q50/00
CPC分类号： G06Q50/00 , G01C21/20 , G01C21/30 , G06Q10/02
摘要： Various tools, strategies and techniques are provided for evaluating the condition of one or more commodities in one or more regions of interest. Collection of image data associated with the commodities can be facilitated through use of an aircraft traveling a predetermined travel route over the regions of interest. The collected image data may be analyzed to evaluate the condition of the commodities, forecast commodity production, and/or to perform other tasks.
摘要翻译：提供各种工具，策略和技术来评估一个或多个感兴趣区域中的一种或多种商品的状况。可以通过使用在感兴趣的区域上行驶预定行进路线的飞机来促进与商品相关联的图像数据的收集。可以分析收集的图像数据以评估商品状况，预测商品生产和/或执行其他任务。

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