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51. 发明授权

US08795998B2 Fermentative glycerol-free ethanol production 有权
标题翻译：发酵甘油无乙醇生产
公开(公告)号：US08795998B2
公开(公告)日：2014-08-05
申请号：US13061695
申请日：2010-07-23
申请人： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
发明人： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
IPC分类号： C12N1/18 , C12N9/08 , C12P7/06 , C12P7/10 , C12N15/81
CPC分类号： C12N15/815 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/93 , C12P7/06 , C12P7/10 , C12Y101/01001 , C12Y101/01008 , C12Y102/0101 , C12Y602/01001 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
摘要翻译：本发明涉及酵母细胞，特别是重组酵母细胞，NADH依赖性甘油合成所需的缺乏酶活性的细胞或相对于NADH依赖性甘油合成具有降低的酶活性的细胞与其相应的野生型酵母细胞，该细胞包含编码NAD +依赖性乙酰化乙醛脱氢酶（EC 1.2.1.10）活性的一种或多种异源核酸序列。本发明还涉及根据本发明的细胞在制备乙醇中的用途。

52. 发明授权

US08715973B1 Organic acid-tolerant microorganisms and uses thereof for producing organic acids 有权
标题翻译：有机耐酸微生物及其用于生产有机酸的用途
公开(公告)号：US08715973B1
公开(公告)日：2014-05-06
申请号：US13798835
申请日：2013-03-13
申请人： Wisconsin Alumni Research Foundation
发明人： Brian Frederick Pfleger , Matthew Brett Begemann
IPC分类号： C12P7/52 , C12P7/40 , C12P7/04 , C12N9/88 , C12P7/42
CPC分类号： C12P7/52 , C12N1/12 , C12N9/93 , C12P7/40 , C12P7/42 , C12Y602/01001
摘要： Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.
摘要翻译：有机耐酸微生物及其使用方法。有机耐酸微生物包括减少或消除AcsA活性或AcsA同系物活性的修饰。这些修饰增加微生物对3-羟基丙酸（3HP），丙烯酸和丙酸等有机酸的耐受性。对微生物的进一步修饰如增加丙二酰辅酶A还原酶和/或乙酰辅酶A羧化酶的表达提供或提高微生物产生3HP的能力。提供了用改性微生物生成有机酸的方法。使用acsA或其同系物作为反选择性标记的方法包括用感兴趣的基因替换细胞中的acsA或其同系物，并且选择含有有效基因的细胞的数量，所述有机酸有效抑制含有acsA或同源物的细胞生长。

53. 发明授权

US08343752B2 Biological methods for preparing adipic acid 有权
标题翻译：制备己二酸的生物学方法
公开(公告)号：US08343752B2
公开(公告)日：2013-01-01
申请号：US13245780
申请日：2011-09-26
申请人： Stephen Picataggio , Tom Beardslee
发明人： Stephen Picataggio , Tom Beardslee
IPC分类号： C12N1/00 , C12N15/00 , C12N15/74 , C12P21/06 , C12P19/34 , C12P7/46 , C12P7/44 , C07H21/04 , C07K1/00
CPC分类号： C12P7/44 , C12N9/001 , C12N9/0042 , C12N9/0073 , C12Y103/03006 , C12Y602/01001 , C12Y602/01003
摘要： The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.
摘要翻译：该技术部分地涉及用于生产己二酸的生物学方法和能够进行这种生产的工程微生物。

54. 发明授权

US08252915B2 Human transferase family members and uses thereof 失效
标题翻译：人转移酶家族成员及其用途
公开(公告)号：US08252915B2
公开(公告)日：2012-08-28
申请号：US12828319
申请日：2010-07-01
申请人： Rachel E. Meyers , John J. Hunter , Fong-Ying Tsai
发明人： Rachel E. Meyers , John J. Hunter , Fong-Ying Tsai
IPC分类号： C07H1/00 , C07H5/04 , C07H5/05 , C07H21/02 , C07H21/04 , C08B37/00 , C12P21/06 , C12N5/00 , C12N5/02 , C12N15/00
CPC分类号： C12Q1/6883 , A01K2217/05 , A61K38/00 , A61K39/00 , C07K14/47 , C07K16/40 , C07K2319/00 , C12N9/1007 , C12N9/1025 , C12N9/1029 , C12N9/1048 , C12N9/1051 , C12N9/1096 , C12N9/93 , C12Q1/48 , C12Q2600/156 , C12Q2600/158 , C12Y602/01001 , C12Y602/01016
摘要： The invention provides isolated nucleic acids molecules, designated 33877, 47179, 26886, 46743, 27417, 32252, and 53320 nucleic acid molecules, which encode novel human transferase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 33877, 47179, 26886, 46743, 27417, 32252, or 53320 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 33877, 47179, 26886, 46743, 27417, 32252, or 53320 gene has been introduced or disrupted. The invention still further provides isolated 33877, 47179, 26886, 46743, 27417, 32252, or 53320 proteins, fusion proteins, antigenic peptides and anti-33877, 47179, 26886, 46743, 27417, 32252, or 53320 antibodies. Diagnostic methods utilizing compositions of the invention are also provided.
摘要翻译：本发明提供编码新的人转移酶家族成员的分离的核酸分子，命名为33877,47179,26886,4673,27417,32252和53320核酸分子。本发明还提供反义核酸分子，含有33877,47179,26886,4673,27417,32252或53320核酸分子的重组表达载体，已经引入了表达载体的宿主细胞，以及非人类转基因动物，其中33877 ，47179,26886,46743,27417,32.252或53320基因已被引入或破坏。本发明还进一步提供了分离的33877,47179,26886,46743,27417,32252或53320蛋白，融合蛋白，抗原肽和抗33877,47179,26886,46743,27417,32252或53320抗体。还提供了利用本发明组合物的诊断方法。

55. 发明申请

US20120122168A1 RECOMBINANT ESCHERICHIA COLI HAVING ENHANCED ACETYL-COENZYME A SYNTHETASE ACTIVITY FOR PRODUCING GLYEROL AND GLYCEROL-DERIVED PRODUCTS 失效
标题翻译：具有增强的乙酰胆碱的重组蛋白合成酶用于生产甘油和甘油衍生产物的合成酶活性
公开(公告)号：US20120122168A1
公开(公告)日：2012-05-17
申请号：US12946120
申请日：2010-11-15
申请人： QI CHEN , QIONG CHENG , ANDREW C. ELIOT , Kristin Ruebling-Jass
发明人： QI CHEN , QIONG CHENG , ANDREW C. ELIOT , Kristin Ruebling-Jass
IPC分类号： C12N1/21 , C12P7/18 , C12P7/42 , C12P7/20
CPC分类号： C12P7/20 , C12N9/93 , C12P7/18 , C12P7/42 , C12P7/52 , C12Y602/01001
摘要： Recombinant E. coli having enhanced acetyl-CoA synthetase activity and the ability to produce glycerol and glycerol-derived products, such as 3-hydroxypropionic acid, methylglyoxal, 1,2-propanediol, and 1,3-propanediol, are described. The recombinant E. coli comprise a promoter operably linked to a nucleotide sequence encoding a polypeptide having acetyl-CoA synthetase enzyme activity, wherein the promoter and nucleotide sequence are each independently either native or non-native.
摘要翻译：描述了具有增强的乙酰辅酶A合成酶活性和产生甘油和甘油衍生产物如3-羟基丙酸，甲基乙二醛，1,2-丙二醇和1,3-丙二醇的能力的重组大肠杆菌。重组大肠杆菌包含与编码具有乙酰-CoA合成酶活性的多肽的核苷酸序列可操作地连接的启动子，其中启动子和核苷酸序列各自独立地是天然的或非天然的。

56. 发明申请

US20110053252A1 MICROORGANISM PRODUCING O-ACETYL-HOMOSERINE AND THE METHOD OF PRODUCING O-ACETYL-HOMOSERINE USING THE MICROORGANISM 有权
标题翻译：生产O-乙酰羟基苯甲酸的微生物和使用微生物生产O-乙酰羟基苯甲酸的方法
公开(公告)号：US20110053252A1
公开(公告)日：2011-03-03
申请号：US12550099
申请日：2009-08-28
申请人： So Young Kim , Yong Uk Shin , In Kyung Heo , Hyun Ah Kim , Chang Il Seo , Ju Eun Kim , Sung Kwang Son , Sang Mok Lee , Sung Hoo Jhon , Han Jin Lee , Kwang Ho Na
发明人： So Young Kim , Yong Uk Shin , In Kyung Heo , Hyun Ah Kim , Chang Il Seo , Ju Eun Kim , Sung Kwang Son , Sang Mok Lee , Sung Hoo Jhon , Han Jin Lee , Kwang Ho Na
IPC分类号： C12N1/21
CPC分类号： C12N9/1205 , C12N9/93 , C12P13/06 , C12Y207/01033 , C12Y602/01001
摘要： Disclosed herein are a microorganism strain capable of producing the L-methionine precursor O-acetyl homoserine in high yield and a method of producing O-acetyl homoserine using the same. The microorganism strain is a strain of Escherichia sp. in which an acetyl-CoA synthase gene (acs) and/or a pantothenate kinase gene (coA) encoding a pantothenate kinase gene refractory to feedback inhibition by CoA is introduced and expressed.
摘要翻译：本文公开了能够以高产率生产L-甲硫氨酸前体O-乙酰高丝氨酸的微生物菌株和使用其的O-乙酰高丝氨酸的生产方法。微生物菌株是大肠杆菌属的菌株。其中引入并表达了编码可由CoA反馈抑制难治的泛酸激酶基因的乙酰辅酶A合成酶基因（acs）和/或泛酸激酶基因（coA）。

57. 发明授权

US07785832B2 Method of protein synthesis 有权
标题翻译：蛋白质合成方法
公开(公告)号：US07785832B2
公开(公告)日：2010-08-31
申请号：US11335592
申请日：2006-01-20
申请人： Jae-Gu Pan , Heung-Chae Jung , Sooan Shin
发明人： Jae-Gu Pan , Heung-Chae Jung , Sooan Shin
IPC分类号： C12P21/00 , C07H21/04
CPC分类号： C12N15/70 , C12N9/78 , C12N9/93 , C12Y602/01001
摘要： The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.
摘要翻译：本发明涉及一种蛋白质合成方法，更具体地说，涉及通过调节宿主细胞中蛋白质表达的有效蛋白质合成方法，其中所述宿主细胞用包含启动子的表达载体以及用于编码所需蛋白质的基因的DNA片段，其中所述启动子在细胞生长的静息期期间具有转录的诱导活性，并且还可以通过改变培养条件来控制所述蛋白质表达的诱导。

58. 发明申请

US20070190605A1 GENE PRODUCTS OF BACILLUS LICHENIFORMIS WHICH FORM ODOROUS SUBSTANCES AND IMPROVED BIOTECHNOLOGICAL PRODUCTION METHODS BASED THEREON 有权
标题翻译：基于生物技术的生物技术生产方法基于生物反应器的基因产物
公开(公告)号：US20070190605A1
公开(公告)日：2007-08-16
申请号：US11616319
申请日：2006-12-27
申请人： Cornelius Bessler , Jorg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
发明人： Cornelius Bessler , Jorg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
IPC分类号： C07H21/04 , C12P21/06 , C12P7/40 , C12N9/02 , C12N9/16 , C12N9/32 , C12N9/42 , C12N9/50 , C12N9/64 , C12N15/74 , C12N1/21
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0016 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12N9/16 , C12N9/78 , C12N9/80 , C12N9/84 , C12N9/88 , C12N9/93 , C12N15/75 , C12P7/16 , C12P7/40 , C12P7/42 , C12P7/52 , C12P13/001 , C12Y101/01 , C12Y101/01035 , C12Y101/01157 , C12Y102/04002 , C12Y103/99 , C12Y203/01019 , C12Y206/01042 , C12Y207/02007 , C12Y305/01001 , C12Y305/01011 , C12Y401/01018 , C12Y401/01019 , C12Y402/01017 , C12Y402/01055 , C12Y602/01 , C12Y602/01001 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.
摘要翻译：本发明涉及地衣芽孢杆菌的25个迄今未描述的基因及其衍生的基因产物及其全部充分同源的核酸及其蛋白质。它们发生在五种不同的代谢途径中，用于形成气味物质。所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分）和/或精氨酸）。这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成用于生物技术生产。此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。

59. 发明公开

US20240254523A1 GENETICALLY ENGINEERED STRAIN FOR PRODUCING POLYLACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID 审中-公开
公开(公告)号：US20240254523A1
公开(公告)日：2024-08-01
申请号：US18607573
申请日：2024-03-18
申请人： SHANGHAI SIPENG TECHNOLOGY LTD.
发明人： Fei TAO , Chunlin TAN , Ping XU
IPC分类号： C12P7/625 , C12N9/00 , C12N9/04 , C12N9/10 , C12N9/12 , C12N15/52
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N9/93 , C12N15/52 , C12Y101/01028 , C12Y203/0118 , C12Y207/02001 , C12Y208/03001 , C12Y602/01001 , C12Y604/01002
摘要： Provided are a genetically engineered strain for producing polylactic acid and a method for producing polylactic acid. The genome of the genetically engineered strain is integrated with a coding sequence of exogenous D-lactate dehydrogenase gene, a coding sequence of exogenous propionyl-CoA transferase gene, and a coding sequence of exogenous polyhydroxyalkanoate synthase gene, enabling the genetically engineered strain to express exogenous D-lactate dehydrogenase, exogenous propionyl-CoA transferase, and exogenous polyhydroxyalkanoate synthase. The method includes: providing the above genetically engineered strain of Synechococcus elongatus; introducing carbon dioxide and culturing the genetically engineered strain under light; and when a growth OD of the genetically engineered strain reaches the maximum, collecting and drying the genetically engineered strain, and recycling the polylactic acid in the strain.

60. 发明授权

US12049661B2 Construction and application of engineered strain of 有权
公开(公告)号：US12049661B2
公开(公告)日：2024-07-30
申请号：US17479018
申请日：2021-09-20
申请人： Jiangnan University
发明人： Liming Liu , Guipeng Hu , Xiulai Chen , Danlei Ma , Jia Liu , Qiuling Luo
IPC分类号： C12P7/46 , C12N1/20 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/12 , C12N9/88 , C12N15/70
CPC分类号： C12P7/46 , C12N1/20 , C12N9/0006 , C12N9/001 , C12N9/0093 , C12N9/1205 , C12N9/88 , C12N9/93 , C12N15/70 , C12Y101/01001 , C12Y101/01028 , C12Y101/0104 , C12Y103/05004 , C12Y117/01 , C12Y207/01029 , C12Y402/01002 , C12Y602/01001
摘要： The disclosure discloses construction and application of an engineered strain of E. coli for producing malic acid by fixing CO2, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation on Escherichia coli MG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with CO2 and glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.

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