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51. 发明申请

US20030135040A1 Compositions and methods of synthesis and use of novel nucleic acid structures 失效
标题翻译：合成和使用新型核酸结构的组合物和方法
公开(公告)号：US20030135040A1
公开(公告)日：2003-07-17
申请号：US10055732
申请日：2002-01-22
发明人： Ramon Eritja , Ramon Guimil Garcia
IPC分类号： C07H019/16
CPC分类号： C12Q1/6839 , C12P19/34 , C12Q2525/173 , C12Q2525/197 , C12Q2525/301 , C12Q2525/113
摘要： The present invention is directed to a method to produce 8-amino-2nulldeoxyadenosine by treating 8-azido-2nulldeoxyadenosine with an aqueous solution of methylamine or dimethylamine.
摘要翻译：本发明涉及通过用甲胺或二甲胺的水溶液处理8-叠氮基-2'-脱氧腺苷来制备8-氨基-2'-脱氧腺苷的方法。

52. 发明授权

US06548251B1 Inhibition of molecular and biological processes using modified oligonucleotides 失效
标题翻译：使用修饰的寡核苷酸抑制分子和生物过程
公开(公告)号：US06548251B1
公开(公告)日：2003-04-15
申请号：US09655804
申请日：2000-09-05
申请人： Sergei A. Kozyavkin , Andrei G. Malykh , Nikolai N. Polouchine , Alexei I. Slesarev
发明人： Sergei A. Kozyavkin , Andrei G. Malykh , Nikolai N. Polouchine , Alexei I. Slesarev
IPC分类号： C12Q168
CPC分类号： C12Q1/6832 , C12Q1/6853 , C12Q2525/113 , C12Q2527/107
摘要： A method of inhibiting at least one molecular process in a sample, comprising administering to the sample an oligonucleotide or polynucleotide containing at least one monomeric unit having formula (I): A—Xn (I) wherein A is an organic moiety, n is at least 1, and each X is independently selected from the group consisting of —NRCOCONu, —NHCOCR2CR2CONu, —NHCOCR═CRCONu, and —NHCOSSCONu, wherein each R independently represents H or a substituted or unsubstituted alkyl group, and Nu represents a nucleophile, or a salt of the compound.
摘要翻译：一种抑制样品中至少一种分子过程的方法，包括向所述样品施用含有至少一个具有式（I）的单体单元的寡核苷酸或多核苷酸：其中A是有机部分，n至少为1，并且每个X 独立地选自-NRCOCONu，-NHCOCR 2 CR 2 CONu，-NHCOCR = CHONONu和-NHCOSSCONu，其中每个R独立地表示H或取代或未取代的烷基，并且Nu表示亲核试剂或化合物的盐。

53. 发明申请

US20030064483A1 Method of nucleic acid sequencing 失效
公开(公告)号：US20030064483A1
公开(公告)日：2003-04-03
申请号：US09984566
申请日：2001-10-30
申请人： DUKE UNIVERSITY.
发明人： Barbara Ramsay Shaw , Kenneth W. Porter , Dmitri Sergueev
IPC分类号： C07H021/04 , C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6869 , C07H21/00 , C12Q1/6806 , C12Q2525/186 , C12Q2525/113 , C12Q2521/319 , C12Q2533/101 , C12Q2525/125
摘要： The present invention relates, in general, to a process of enzymatically synthesizing nucleic acids containing nucleotides that are resistant to degradation. The invention further relates to methods of utilizing such nucleic acids in DNA and RNA amplification and sequencing, gene therapy and molecular detection protocols.

54. 发明授权

US06514693B1 Method for detecting multiple copies of a repeat sequence in a nucleic acid molecule 失效
公开(公告)号：US06514693B1
公开(公告)日：2003-02-04
申请号：US08730635
申请日：1996-10-11
申请人： Peter Lansdorp
发明人： Peter Lansdorp
IPC分类号： C12Q168
CPC分类号： C12Q1/6876 , C12Q1/6841 , C12Q1/6886 , C12Q2600/136 , C12Q2525/151 , C12Q2525/113 , C12Q2525/107
摘要： A method for detecting or quantitating multiple copies of a repeat sequence in a nucleic acid molecule involving treating the nucleic acid molecule with a probe which is a nucleic acid analogue which is capable of hybridizing to the repeat sequence in the nucleic acid molecule and which is labelled with a detectable substance. The nucleic acid molecule is treated with the probe under conditions permitting the probe to hybridize to the repeat sequences in the nucleic acid molecule. Probe hybridized to complementary repeat sequences is identified in the nucleic acid molecule by directly or indirectly detecting the detectable substance. The method is preferably used for quantitating multiple copies of a repeat sequence in a nucleic acid molecule, preferably a telomere or centromere repeat sequence. Novel probes for use in the method of the invention and kits are described.

55. 发明申请

US20030022204A1 Method for detecting multiple copies of a repeat sequence in a nucleic acid molecule 审中-公开
标题翻译：用于检测核酸分子中重复序列的多个拷贝的方法
公开(公告)号：US20030022204A1
公开(公告)日：2003-01-30
申请号：US10132002
申请日：2002-04-25
发明人： Peter Lansdorp
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6876 , C12Q1/6841 , C12Q1/6886 , C12Q2600/136 , C12Q2525/151 , C12Q2525/113 , C12Q2525/107
摘要： A method for detecting or quantitating multiple copies of a repeat sequence in a nucleic acid molecule involving treating the nucleic acid molecule with a probe which is a nucleic acid analogue which is capable of hybridizing to the repeat sequence in the nucleic acid molecule and which is labelled with a detectable substance. The nucleic acid molecule is treated with the probe under conditions permitting the probe to hybridize to the repeat sequences in the nucleic acid molecule. Probe hybridized to complementary repeat sequences is identified in the nucleic acid molecule by directly or indirectly detecting the detectable substance. The method is preferably used for quantitating multiple copies of a repeat sequence in a nucleic acid molecule, preferably a telomere or centromere repeat sequence. Novel probes for use in the method of the invention and kits are described.
摘要翻译：一种用于检测或定量核酸分子中重复序列的多个拷贝的方法，包括用能够与核酸分子中的重复序列杂交的核酸类似物的探针来处理核酸分子，并且被标记具有可检测物质。在允许探针与核酸分子中的重复序列杂交的条件下用探针处理核酸分子。通过直接或间接检测可检测物质，在核酸分子中鉴定与互补重复序列杂交的探针。该方法优选用于在核酸分子，优选端粒或着丝粒重复序列中定量重复序列的多个拷贝。描述了用于本发明方法和试剂盒的新型探针。

56. 发明申请

US20020022228A1 Method and test kit for analyzing DNA repair 审中-公开
标题翻译：分析DNA修复的方法和试剂盒
公开(公告)号：US20020022228A1
公开(公告)日：2002-02-21
申请号：US09848116
申请日：2001-04-30
发明人： Peter Nehls , Karl Heinz Glusenkamp
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12Q1/6834 , C12Q2563/131 , C12Q2525/113
摘要： A method for analyzing the repair of DNA modifications and base mispairings as well as apurinic and apyrimidinic sites by DNA repair enzymes, comprising the following steps: contacting (a) single- or double-stranded DNA molecules which were covalently coupled to a solid-phase matrix carrying primary or secondary amino groups by reaction with a reactive squaric acid derivative, and which have modifications and/or base mispairings and/or apurinic or apyrimidinic sites, with (b) a composition containing DNA repair enzymes; and determining the elimination of the DNA modifications and/or base mispairings and/or apurinic or apyrimidinic sites. The DNA molecules are covalently coupled to the solid state matrix via a primary or secondary amino group incorporated in the DNA molecule at the 5null-end or at the 3null-end of the DNA or in the 2null-position of at least one deoxyribosyl residue.
摘要翻译：一种通过DNA修复酶分析DNA修饰和碱基错配的修复以及脱嘌呤和无嘧啶位点的方法，包括以下步骤：使（a）共价偶联到固相的单链或双链DNA分子通过与反应性方酸衍生物反应并具有修饰和/或碱基错配和/或脱嘌呤或嘧啶位点的基质携带伯或仲氨基，（b）含有DNA修复酶的组合物; 并确定DNA修饰和/或碱基错配和/或脱嘌呤或无嘧啶基位点的消除。 DNA分子通过引入到DNA分子中的DNA或分子中的一个或多个氨基基团共价偶联到DNA的5'端或3'端，或者在至少一个脱氧核糖基残基。

57. 发明申请

US20020012902A1 METHODS AND KIT FOR HYBRIDIZATION ANALYSIS USING PEPTIDE NUCLEIC ACID PROBES 无效
标题翻译：使用肽核酸探针进行杂交分析的方法和工具包
公开(公告)号：US20020012902A1
公开(公告)日：2002-01-31
申请号：US08726093
申请日：1996-10-04
发明人： MARTIN FUCHS , MICHAEL EGHOLM , HEATHER O'KEEFE , XIAN-WEI YAO
IPC分类号： C12Q001/00 , G01N031/00 , G01N033/53 , C25D021/12
CPC分类号： G01N27/44726 , C12Q1/6813 , G01N27/447 , C12Q2525/113 , C12Q2523/113 , C12Q2565/125 , C12Q2565/629 , C12Q2565/107
摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.
摘要翻译：一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在）将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。

58. 发明授权

US06251591B1 Quantitative method for detecting nucleotide concentration 失效
标题翻译：检测核苷酸浓度的定量方法
公开(公告)号：US06251591B1
公开(公告)日：2001-06-26
申请号：US09083837
申请日：1998-05-22
申请人： Yuan Min Wu , Eileen Xiao-Feng Nie
发明人： Yuan Min Wu , Eileen Xiao-Feng Nie
IPC分类号： C12Q168
CPC分类号： C12Q1/6813 , C12Q2527/143 , C12Q2525/113
摘要： The invention provides a method for rapidly, economically and efficiently determining the concentration of a target nucleobase-containing sequence in a fluid medium using laser induced fluorescence of antisense probes. When hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the test medium is proportional to the concentration of the target sequence. When hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the test medium is inversely proportional to the concentration of the target sequence. The method can be used to determine the concentration of a contaminant in a sample as a part of a system of quality control.
摘要翻译：本发明提供了使用反射探针的激光诱导荧光来快速，经济且有效地测定流体介质中靶核苷酸序列的浓度的方法。当杂交复合物和未杂交的探针在检测前分离时，测试培养基的荧光强度与靶序列的浓度成比例。当杂交复合物和未杂交的探针在检测之前未分离时，测试培养基的荧光强度与靶序列的浓度成反比。该方法可用于确定样品中污染物的浓度作为质量控制系统的一部分。

59. 发明授权

US06232462B1 Reduction of nonspecific hybridization by using novel base-pairing schemes 失效
标题翻译：通过使用新的碱基配对方案减少非特异性杂交
公开(公告)号：US06232462B1
公开(公告)日：2001-05-15
申请号：US09115566
申请日：1998-07-14
申请人： Mark L. Collins , Thomas Horn , Patrick J Sheridan , Brian D. Warner , Michael S. Urdea
发明人： Mark L. Collins , Thomas Horn , Patrick J Sheridan , Brian D. Warner , Michael S. Urdea
IPC分类号： C07H2100
CPC分类号： C12N15/113 , C07H19/16 , C07H19/20 , C07H21/00 , C12N2310/322 , C12N2310/336 , C12Q1/68 , C12Q1/6811 , C12Q1/6813 , C12Q1/682 , C12Q1/6832 , C12Q1/6837 , C12Q2527/107 , C12Q2525/117 , C12Q2525/113
摘要： Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2′-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.
摘要翻译：提供了用于基本上减少在核酸杂交测定中遇到的背景信号的方法。该方法的前提是消除或显着降低非特异性杂交的现象，从而提供仅在存在目标目的多核苷酸的情况下产生的可检测信号。另外，提供了一种用于化学合成异鸟嘌呤或2'-脱氧 - 异鸟嘌呤的新方法。本发明还可应用于反义和适体治疗剂和药物发现。

60. 发明申请

US20180171393A1 MONITORING AND ANALYSIS OF NUCLEIC ACID HYBRIDIZATION STATE AND AMPLIFICATION USING L-DNA 审中-公开
公开(公告)号：US20180171393A1
公开(公告)日：2018-06-21
申请号：US15571153
申请日：2016-05-02
申请人： VANDERBILT UNIVERSITY , BioVentures, Inc.
发明人： Frederick R. Haselton , Nicholas M. Adams , Steven J. Simmons , Elliott P. Dawson
IPC分类号： C12Q1/6818 , C12Q1/686 , B01L7/00
CPC分类号： C12Q1/6818 , B01L7/52 , C12N2310/122 , C12N2310/32 , C12Q1/68 , C12Q1/686 , C12Q2525/101 , C12Q2525/113 , C12Q2531/113 , C12Q2537/143 , C12Q2561/113 , Y02A50/58
摘要： Systems, methods, and compositions for monitoring and analyzing nucleic acid hybridization state using L-DNA probes are described. The methods include adding L-DNA probes that can be fluorescently detected to a system including D-DNA. The L-DNA probes include primer, target, and antisense nucleotide sequences, and fluorescent dye compounds. The L-DNA probes are particularly useful for monitoring and analyzing various parameters during DNA amplification using the polymerase chain reaction.

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