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    • 55. 发明授权
    • Methods for the detoxification of nitrile and/or amide compounds
    • 腈和/或酰胺化合物的解毒方法
    • US5863750A
    • 1999-01-26
    • US769080
    • 1996-12-18
    • George E. Pierce
    • George E. Pierce
    • C12M1/00C02F3/00C02F3/12C02F3/34C12N1/00C12N11/14C12Q1/02C12Q1/00C12Q1/24C12Q33/53
    • C02F3/1231C02F3/34Y02W10/15Y10S435/81Y10S435/843Y10S435/975
    • The present invention describes methods for the detoxification of a mixture of nitrile compounds, or a mixture of nitrile and amide compounds by conversion of the nitrile compound(s) to the corresponding amide or acid compounds using a pure culture of an induced microorganism strain capable of converting a nitrile moiety to an amide or acid moiety. If an amide is formed or is present in the mixture, the amide can be further converted, using the present methods for detoxification, to the corresponding acid. The acid can then, if desired, be further degraded to CO.sub.2, H.sub.2 O and biomass. The induced pure cultures are able to detoxify a mixture of nitrites or a mixture of nitriles and amides which are typically present, in high concentration(s), in nitrile production waste streams. The present invention further discloses methods for removing a nitrile compound from an amide preparation, such as an acetamide or acrylamide preparation containing an unwanted nitrile compound, using an induced pure culture of an induced microorganism strain capable of converting a nitrile moiety to an amide or acid moiety. The pure cultures are able to purify or reduce the toxicity of the amide preparation thus improving purity and amide product yield from the amide preparation. The present invention further discloses methods for the conversion of a mixture of amide compounds to the corresponding acid compounds using a pure culture of an induced microorganism strain capable of converting an amide moiety to an acid moiety. This invention also discloses kits, biofilters and methods for use of the kits and biofilters for detoxification containing the useful microorganism strains.
    • 本发明描述了通过将纯化学培养的腈衍生物转化成相应的酰胺或酸化合物来对腈化合物或腈和酰胺化合物的混合物进行解毒的方法, 将腈部分转化为酰胺或酸部分。 如果形成酰胺或存在于混合物中,则可以使用本发明的解毒方法将酰胺进一步转化成相应的酸。 如果需要,酸可以进一步降解为CO 2,H 2 O和生物质。 诱导的纯培养物能够在腈生产废物流中以高浓度存在的亚硝酸盐或腈和酰胺的混合物的混合物解毒。 本发明还公开了使用能够将腈部分转化为酰胺或酸的诱导微生物菌株的诱导纯培养物从酰胺制剂例如乙酰胺或含有不需要的腈化合物的丙烯酰胺制剂中除去腈化合物的方法 部分。 纯培养物能够净化或降低酰胺制剂的毒性,从而提高酰胺制剂的纯度和酰胺产物产率。 本发明还公开了使用能够将酰胺部分转化为酸部分的诱导微生物菌株的纯培养物将酰胺化合物的混合物转化为相应的酸化合物的方法。 本发明还公开了用于该试剂盒和生物过滤器的用于解毒的试剂盒,生物过滤器和方法,其含有有用的微生物菌株。