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51. 发明申请

US20070059763A1 Cellomics system 失效
标题翻译：大提琴系统
公开(公告)号：US20070059763A1
公开(公告)日：2007-03-15
申请号：US11195662
申请日：2005-08-03
申请人： Kazunori Okano , Kenji Yasuda
发明人： Kazunori Okano , Kenji Yasuda
IPC分类号： G01N33/53
CPC分类号： G01N33/5061 , G01N33/566
摘要： In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.
摘要翻译：在标记细胞并根据使用细胞分离器的标记程度分离和收集细胞时，对细胞的影响被最小化，并且促进使用所收集的细胞，从而当标记细胞时，细胞被标记在保持每个单元的相互作用的状态下。在标记中，存在于靶细胞表面上的特定标记材料经由转运体取出，细胞逐个分散，将其与细胞分离器分离。分离后立即将细胞置于不含特定标记物质的溶液中以除去细胞中所含的特异性标记物质。该系列步骤用细胞分离芯片连续进行。

52. 发明申请

US20060183118A1 Nucleic acid recovery chip and nucleic acid recovery device 审中-公开
标题翻译：核酸回收芯片和核酸回收装置
公开(公告)号：US20060183118A1
公开(公告)日：2006-08-17
申请号：US10525404
申请日：2003-08-26
申请人： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
发明人： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12M35/02 , C07H21/00
摘要： On a glass plate being transparent at specified wavelengths there are provided a laminated region being absorptive at the specified wavelengths; means for applying voltage to the region, the region exhibiting electrical conductivity; means for binding nucleic acids onto the region; a container for accommodating cells on the region; means for culturing cells in the container; means for observing the cells; and means for effecting localized dissociation and recovery of nucleic acid components bound on the region by heat, the heat generated locally only in the vicinity of focused light by irradiating the region with focused light of the specified wavelengths. For clarifying the distribution of nucleic acid components in cells of specified condition or the distribution of nucleic acid components in each cell of a tissue cell mass there is provided means for selectively separating and recovering nucleic acid components of specified range in each cell of specified cellular condition.
摘要翻译：在特定波长透明的玻璃板上，提供在特定波长下吸收的层叠区域; 用于向该区域施加电压的装置，显示导电性的区域; 用于将核酸结合到该区域上的方法; 用于容纳所述区域上的细胞的容器; 用于在容器中培养细胞的方法; 观察细胞的手段; 以及用于通过加热在区域上结合的核酸组分的局部解离和回收的手段，通过用指定波长的聚焦光照射该区域，局部仅在聚焦光附近产生的热。为了澄清核酸组分在特定条件的细胞中的分布或组织细胞块的每个细胞中的核酸组分的分布，提供了用于在特定细胞条件的每个细胞中选择性分离和回收特定范围的核酸组分的手段。

53. 发明申请

US20060141618A1 Cell separation apparatus 失效
标题翻译：细胞分离装置
公开(公告)号：US20060141618A1
公开(公告)日：2006-06-29
申请号：US10543867
申请日：2004-04-30
申请人： Kenji Yasuda , Kazunori Takahashi
发明人： Kenji Yasuda , Kazunori Takahashi
IPC分类号： C12M1/00 , C12N5/06
CPC分类号： C12M47/04 , B01L3/502761 , B01L2300/0681 , B01L2400/0421 , B01L2400/043 , B01L2400/0439 , B01L2400/086
摘要： A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the exhaustion of an electrode due to its electrolysis.
摘要翻译：细胞分离装置包括用于通过使用凝胶电极向细胞分离空间中的细胞施加电压而使细胞分离空间内的细胞移动的装置和用于分离和排出细胞的通道，因此不破坏细胞样品，防止由于电解而导致电极的耗尽。

54. 发明申请

US20060134667A1 Method for detecting fusion gene 审中-公开
标题翻译：融合基因检测方法
公开(公告)号：US20060134667A1
公开(公告)日：2006-06-22
申请号：US11274314
申请日：2005-11-16
申请人： Masatoshi Narahara , Toshiro Saito , Takayuki Obara , Kenji Yasuda
发明人： Masatoshi Narahara , Toshiro Saito , Takayuki Obara , Kenji Yasuda
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/6834 , C12Q1/6886 , C12Q2600/158 , C12Q2525/143 , C12Q2521/119 , C12Q2525/149
摘要： The present invention relates to a method for detecting fusion gene transcripts resulting from chromosomal translocation. Specifically, the method of the present invention comprises allowing at least two or more probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or complementary base sequence thereof, and each of which immobilized on a support, to hybridize with a sample containing a nucleic acid derived from a fusion gene, thereby allowing the detection of two or more fusion genes at a time.
摘要翻译：本发明涉及一种检测由染色体易位引起的融合基因转录物的方法。具体地说，本发明的方法包括允许至少两个或更多个探针，每个探针含有夹心融合基因或其互补碱基序列的断点的外显子的部分碱基序列，并且每个探针固定在载体上，与含有衍生自融合基因的核酸的样品杂交，从而一次检测两个或更多个融合基因。

55. 发明授权

US07064813B2 Apparatus and method for measuring micro area in specimen 失效
标题翻译：用于测量样品中微区的装置和方法
公开(公告)号：US07064813B2
公开(公告)日：2006-06-20
申请号：US10253479
申请日：2002-09-25
申请人： Satoshi Takahashi , Kenji Yasuda , Yoshitada Oshida , Taisaku Seino
发明人： Satoshi Takahashi , Kenji Yasuda , Yoshitada Oshida , Taisaku Seino
IPC分类号： G01N21/00
CPC分类号： G01N1/312
摘要： Disclosed is an apparatus for measuring a micro area in a specimen in which a reagent necessary for observation and examination of a cell smear specimen or a tissue specimen is dropped to cause a reaction for a measurement and an analysis by way of an image. The apparatus includes: a micro reaction unit that is able to select a specific micro area in the specimen and to subject the specific micro area to a reaction-operation; a unit that measures; records and displays an image of the micro area; and a control unit that controls the measuring, recording, and displaying unit. Reactions can be effected quickly on such specimens as smear cell specimens and tissue slice specimens. The application of the reagent solution can be saved. A comparison between local presence of a gene or anti-body and a cell image can be made quickly and easily.
摘要翻译：公开了一种用于测量样本中的微小区域的装置，其中用于观察和检查细胞涂片或组织样本的试剂下降以引起测量反应和通过图像进行分析。该装置包括：微反应单元，其能够选择样品中的特定微区域并使特定微区域进行反应操作; 衡量单位记录并显示微区图像; 以及控制单元，其控制测量，记录和显示单元。反应可以在诸如涂片细胞标本和组织切片标本的样品上快速实现。可以节省试剂溶液的应用。基因或抗体的局部存在与细胞图像的比较可以快速且容易地进行。

56. 发明申请

US20060127378A1 Composition for promoting the proliferation of lactobacillus casei subsp. casei 审中-公开
标题翻译：用于促进干酪乳杆菌亚种增殖的组合物干酪
公开(公告)号：US20060127378A1
公开(公告)日：2006-06-15
申请号：US10521947
申请日：2003-07-22
申请人： Kenji Yasuda , Tomohiko Ogawa , Masakatsu Hasegawa
发明人： Kenji Yasuda , Tomohiko Ogawa , Masakatsu Hasegawa
IPC分类号： A61K35/74 , A61K31/721
CPC分类号： C12N1/38 , A23K20/163 , A23L29/273 , A23L33/135 , A23V2002/00 , C12N1/20 , A23V2200/3204 , A23V2250/5042 , A23V2200/3202
摘要： The present invention relates to a composition for promoting bacterial proliferation for selectively proliferating Lactobacillus casei subsp. casei, which includes a dextran. According to the present invention, a variety of biological activities originated from L. casei subsp. casei can be sustained in a living body by selectively growing-proliferating and colonizing L. casei subsp. casei in the intestine of a human being, animal, or the like or by selectively growing-proliferating L. casei subsp. casei in the intestine, without supplying L. casei subsp. casei at all times.
摘要翻译：本发明涉及用于促进细菌增殖以选择性增殖干酪乳杆菌亚种的组合物。其中包括葡聚糖。根据本发明，起源于干酪乳杆菌的各种生物活性。干酪素可以通过选择性生长增殖和殖民化的干酪乳杆菌亚种，维持在活体内。在人，动物等的肠中或通过选择性增殖增殖的干酪乳杆菌肠道中的酪蛋白，不提供干酪乳杆菌。干酪素在任何时候。

57. 发明申请

US20050117167A1 Light interference measurement method using computer-generated hologram, and interferometer using this method 失效
标题翻译：使用计算机生成的全息图的光干涉测量方法，以及使用该方法的干涉仪
公开(公告)号：US20050117167A1
公开(公告)日：2005-06-02
申请号：US10994270
申请日：2004-11-23
申请人： Kenji Yasuda , Shinichi Matsuda
发明人： Kenji Yasuda , Shinichi Matsuda
IPC分类号： G01B9/02 , G01B9/023 , G01B11/24 , G03H1/00 , G03H1/08
CPC分类号： G03H1/0005 , G01B9/023 , G01B11/2441 , G03H1/08 , G03H2001/0033 , G03H2001/0066 , G03H2001/0072
摘要： The present invention makes it possible to reliably hold a computer-generated hologram 17 and a sample surface 18a in ideal design positions and to perform interference measurements on the surface shape of the sample surface 18a by holding the computer-generated hologram 17 in a predetermined attitude by using a retaining member, and interposing a spacer 45 between this retaining member and the object so that relative positioning of both the computer-generated hologram 17 and the sample surface 18a is performed. Light transmission type targets used for alignment are respectively disposed on the four corners of the frame body of the CGH 17 and the four corners of the measurement mirror 18, and the system is devised so that images of these targets can be obtained by an image focusing lens 43 and CCD camera 44, thus making it possible to check the adjustment of the alignment of the CGH 17 and sample surface 18a.
摘要翻译：本发明使得可以将计算机生成的全息图17和样品表面18a可靠地保持在理想的设计位置，并且通过将计算机产生的全息图17保持在样品表面18a中来对样品表面18a的表面形状进行干涉测量通过使用保持构件的预定姿态，并且在该保持构件和物体之间插入间隔件45，使得执行计算机生成的全息图17和样本表面18a的相对定位。用于对准的光透射型目标分别设置在CGH 17的框体和测量镜18的四个角的四个角上，并且设计了系统，使得可以通过图像聚焦获得这些目标的图像透镜43和CCD照相机44，从而可以检查CGH 17和样品表面18a的对准的调整。

58. 发明授权

US06787314B2 Polynucleotide separation method and apparatus therefor 失效
标题翻译：多核苷酸分离方法及其设备
公开(公告)号：US06787314B2
公开(公告)日：2004-09-07
申请号：US10003305
申请日：2001-12-06
申请人： Kenji Yasuda , Kazunori Okano , Hirokazu Kato
发明人： Kenji Yasuda , Kazunori Okano , Hirokazu Kato
IPC分类号： C12Q168
CPC分类号： B01L3/5027 , B01J2219/0052 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L3/50851 , B01L7/52 , B01L7/54 , B01L2200/0668 , B01L2200/0673 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0825 , B01L2300/0838 , B01L2300/0877 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , B01L2400/0421 , B01L2400/0454 , B01L2400/0487 , C12Q1/6806 , C12Q1/6837 , C40B40/06
摘要： Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.
摘要翻译：将各自具有特定碱基序列的不同探针固定在形成在基底表面上的每个独立区域上，将样品溶液中的互补多核苷酸与探针杂交，并将基底上的每个独立区域加热然后冷却序列，因此回收溶液以提取分开对应于各个探针的不同多核苷酸。

59. 发明授权

US06245207B1 Cell separation device using ultrasound and electrophoresis 失效
标题翻译：细胞分离装置采用超声波和电泳
公开(公告)号：US06245207B1
公开(公告)日：2001-06-12
申请号：US09313212
申请日：1999-05-18
申请人： Kenji Yasuda , Takeshi Sakamoto
发明人： Kenji Yasuda , Takeshi Sakamoto
IPC分类号： C02F140
CPC分类号： B01D21/283 , B01D21/0009 , B03D3/04 , C02F1/36 , C02F1/469 , C12M47/04 , G01N27/44769
摘要： A cell separation module for separating and collecting cells having a charge and cells having no charge comprises a holder and a throwaway cell separation chamber held by the holder. Ultrasound is introduced into the cell separation chamber so as to allow force for causing cells to be put together to act into the cell separation chamber. In addition, an electrostatic force is allowed to act in the cell separation chamber so as to cause the cell to move by the charges of the cells. Competition between both the forces is used to separate the cells correspondingly to the freshness of the cells.
摘要翻译：用于分离和收集具有电荷的细胞和不含电荷的细胞的细胞分离模块包括保持器和由保持器保持的一次性细胞分离室。将超声波引入细胞分离室，以便允许将细胞置于一起作用于细胞分离室的力。此外，允许静电力作用于细胞分离室，以使细胞通过细胞的电荷而移动。使用两个力之间的竞争来对应于细胞的新鲜度分离细胞。

60. 发明授权

US06218126B1 Polynucleotide separation method and apparatus therefor 失效
标题翻译：多核苷酸分离方法及其设备
公开(公告)号：US06218126B1
公开(公告)日：2001-04-17
申请号：US09522465
申请日：2000-03-09
申请人： Kenji Yasuda , Kazunori Okano , Hirokazu Kato
发明人： Kenji Yasuda , Kazunori Okano , Hirokazu Kato
IPC分类号： C12Q168
CPC分类号： B01L3/5027 , B01J2219/0052 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L3/50851 , B01L7/52 , B01L7/54 , B01L2200/0668 , B01L2200/0673 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0825 , B01L2300/0838 , B01L2300/0877 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , B01L2400/0421 , B01L2400/0454 , B01L2400/0487 , C12Q1/6806 , C12Q1/6837 , C40B40/06
摘要： Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.
摘要翻译：将各自具有特定碱基序列的不同探针固定在形成在基底表面上的每个独立区域上，将样品溶液中的互补多核苷酸与探针杂交，并将基底上的每个独立区域加热然后冷却序列，因此回收溶液以提取分开对应于各个探针的不同多核苷酸。

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