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51. 发明授权

US08282688B2 Laccase mediators and methods of use 有权
标题翻译：漆酶介体和使用方法
公开(公告)号：US08282688B2
公开(公告)日：2012-10-09
申请号：US11954863
申请日：2007-12-12
申请人： Joseph C. McAuliffe , Huaming Wang
发明人： Joseph C. McAuliffe , Huaming Wang
IPC分类号： D06L3/11
CPC分类号： D06P5/158 , C12N9/0061 , C12Y110/03002 , D06P5/13 , D06P5/132 , D06P5/137 , D06P5/15 , D06P5/153
摘要： Novel laccase mediators, including carboxyamido and cyano derivatives of 2,6-dimethoxyphenol that exhibit improved hydrolytic stability and good bleaching performance. The novel laccase enzymes may be employed in conjunction with the 2,6-dimethoxyphenol derivatives of this invention to provide an improved method for bleaching denim fabrics.
摘要翻译：新型漆酶介质，包括显示出改进的水解稳定性和良好漂白性能的2,6-二甲氧基苯酚的羧酰氨基和氰基衍生物。新型漆酶可以与本发明的2,6-二甲氧基苯酚衍生物结合使用，以提供漂白牛仔布织物的改进方法。

52. 发明申请

US20080248527A1 Binding Phenol Oxidizing Enzyme-Peptide Complexes 审中-公开
标题翻译：结合酚氧化酶 - 肽复合物
公开(公告)号：US20080248527A1
公开(公告)日：2008-10-09
申请号：US12133247
申请日：2008-06-04
申请人： Aehle Wolfgang , Toby M. Baldwin , Franciscus J. C. Van Gastel , Giselle G. Janssen , Christopher J. Murray , Huaming Wang , Deborah S. Winetzky
发明人： Aehle Wolfgang , Toby M. Baldwin , Franciscus J. C. Van Gastel , Giselle G. Janssen , Christopher J. Murray , Huaming Wang , Deborah S. Winetzky
IPC分类号： D06M16/00 , C07K7/00 , C12N15/11 , C12N15/00 , C12N9/02 , C12N1/00 , C11D7/42 , C12P21/04
CPC分类号： C11D3/38681 , C12N9/0061
摘要： The present application relates to peptides which bind to a target stain, phenol oxidizing enzyme-binding peptide complexes wherein the binding peptide is attached to the C-terminus of the phenol oxidizing enzyme or is inserted or substituted into the phenol oxidizing enzyme. In a preferred embodiment the phenol oxidizing enzyme is a laccase specifically Stachybotrys oxidase B and variants thereof. The invention provides expression vectors comprising the phenol oxidizing enzyme-binding peptide complex as well as host cells comprising the vectors.
摘要翻译：本申请涉及与靶染色剂结合的肽，酚氧化酶结合肽复合物，其中结合肽连接到酚氧化酶的C末端或插入或取代到酚氧化酶中。在优选的实施方案中，酚氧化酶是漆酶特异性Stachybotrys氧化酶B及其变体。本发明提供了包含酚氧化酶结合肽复合物的表达载体以及包含载体的宿主细胞。

53. 发明申请

US20080234465A1 EXPRESSION IN FILAMENTOUS FUNGI OF PROTEASE INHIBITORS AND VARIANTS THEREOF 有权
标题翻译：蛋白酶抑制剂及其变体的真菌真菌中的表达
公开(公告)号：US20080234465A1
公开(公告)日：2008-09-25
申请号：US11848196
申请日：2007-08-30
申请人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
发明人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
IPC分类号： C07K14/00 , C07K1/00
CPC分类号： C12N15/80 , C07K14/811
摘要： Described herein are protease inhibitors, variants thereof and methods for their production.
摘要翻译：本文描述的是蛋白酶抑制剂，其变体及其制备方法。

54. 发明授权

US07402664B2 Nucleic acids and expression vectors comprising carotenoid binding peptides 有权
标题翻译：核酸和包含类胡萝卜素结合肽的表达载体
公开(公告)号：US07402664B2
公开(公告)日：2008-07-22
申请号：US10235043
申请日：2002-09-03
申请人： Aehle Wolfgang , Toby M. Baldwin , Franciscus J. C. van Gastel , Giselle G. Janssen , Christopher J. Murray , Huaming Wang , Deborah S. Winetzky
发明人： Aehle Wolfgang , Toby M. Baldwin , Franciscus J. C. van Gastel , Giselle G. Janssen , Christopher J. Murray , Huaming Wang , Deborah S. Winetzky
IPC分类号： C07H21/02 , C07H21/04 , A61K38/04 , A61K38/00 , C12N15/00
CPC分类号： C11D3/38681 , C12N9/0061
摘要： The present application relates to peptides which bind to a target stain, phenol oxidizing enzyme-binding peptide complexes wherein the binding peptide is attached to the C-terminus of the phenol oxidizing enzyme or is inserted or substituted into the phenol oxidizing enzyme. In a preferred embodiment the phenol oxidizing enzyme is a laccase specifically Stachybotrys oxidase B and variants thereof. The invention provides expression vectors comprising the phenol oxidizing enzyme-binding peptide complex as well as host cells comprising the vectors.
摘要翻译：本申请涉及与靶染色剂结合的肽，酚氧化酶结合肽复合物，其中结合肽连接到酚氧化酶的C末端或插入或取代到酚氧化酶中。在优选的实施方案中，酚氧化酶是漆酶特异性Stachybotrys氧化酶B及其变体。本发明提供了包含酚氧化酶结合肽复合物的表达载体以及包含载体的宿主细胞。

55. 发明授权

US07378256B2 Aspergillus niger promoter for expressing genes in host cells 有权
标题翻译：用于在宿主细胞中表达基因的黑曲霉启动子
公开(公告)号：US07378256B2
公开(公告)日：2008-05-27
申请号：US10992149
申请日：2004-11-18
申请人： Steve Kim , Edwin Lee , Wei Liu , Huaming Wang
发明人： Steve Kim , Edwin Lee , Wei Liu , Huaming Wang
IPC分类号： C12P21/02
CPC分类号： C12N15/81 , C07K14/38
摘要： The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same.
摘要翻译：本发明涉及分离的启动子序列，特别是从本文称为A4-L或A4的黑曲霉分离的启动子以及DNA构建体和包含其的载体。

56. 发明授权

US07160709B2 Phenol oxidizing enzymes 有权
标题翻译：苯酚氧化酶
公开(公告)号：US07160709B2
公开(公告)日：2007-01-09
申请号：US10997247
申请日：2004-11-24
申请人： Huaming Wang
发明人： Huaming Wang
IPC分类号： C12N9/02 , C12N15/53 , C12N15/79
CPC分类号： C11D3/38654 , C12N9/0057
摘要： Disclosed herein are novel phenol oxidizing enzymes naturally-produced by strains of the species Stachybotrys which possess a pH optima in the alkaline range and which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes.Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts.
摘要翻译：本文公开了由Stachybotrys品种天然产生的新型酚氧化酶，其在碱性范围内具有最佳pH，可用于改变与染料和有色化合物相关的颜色以及抗染料转移应用。本文还公开了Stachybotrys属（Stachybotrys parvispora MUCL 38996）和Stachybotrys chartarum MUCL 38898属的能够天然产生新型酚氧化酶的菌株的生物纯培养物。本文公开了Stachybotrys酚氧化酶的氨基酸和核酸序列以及包含该核酸的表达载体和宿主细胞。本文公开了生产酚氧化酶的方法以及构建表达宿主的方法。

57. 发明申请

US20060154843A1 Neutral cellulase catalytic core and method of producing same 审中-公开
公开(公告)号：US20060154843A1
公开(公告)日：2006-07-13
申请号：US11305650
申请日：2005-12-16
申请人： Huaming Wang , Kai Bao
发明人： Huaming Wang , Kai Bao
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12N9/42 , C12N15/74 , C12N1/21
CPC分类号： C12N9/2437 , A23K10/14 , A23K20/189 , C11D3/38645 , D06M16/003 , D06M2101/06 , D06M2200/50 , D06P1/38 , D21C5/005
摘要： The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

58. 发明申请

US20060105425A1 Aspergillus niger promoter for expressing genes in host cells 有权
标题翻译：用于在宿主细胞中表达基因的黑曲霉启动子
公开(公告)号：US20060105425A1
公开(公告)日：2006-05-18
申请号：US10992149
申请日：2004-11-18
申请人： Steve Kim , Edwin Lee , Wei Liu , Huaming Wang
发明人： Steve Kim , Edwin Lee , Wei Liu , Huaming Wang
IPC分类号： C12P21/06 , C07H21/04 , C12N9/00 , C12N15/74 , C12N1/21
CPC分类号： C12N15/81 , C07K14/38
摘要： The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same.
摘要翻译：本发明涉及分离的启动子序列，特别是从本文称为A4-L或A4的黑曲霉分离的启动子以及DNA构建体和包含其的载体。

59. 发明申请

US20050244923A1 Novel phenol oxidizing enzymes 有权
标题翻译：新型酚氧化酶
公开(公告)号：US20050244923A1
公开(公告)日：2005-11-03
申请号：US10997247
申请日：2004-11-24
申请人： Huaming Wang
发明人： Huaming Wang
IPC分类号： C12N15/09 , C07H21/04 , C11D3/386 , C12N1/15 , C12N1/16 , C12N1/18 , C12N1/19 , C12N1/21 , C12N9/02 , C12N15/53 , C12N15/74 , C12P21/00 , C12P21/06 , C12R1/07 , C12R1/645 , C12R1/66 , C12R1/85
CPC分类号： C11D3/38654 , C12N9/0057
摘要： Disclosed herein are novel phenol oxidizing enzymes naturally-produced by strains of the species Stachybotrys which possess a pH optima in the alkaline range and which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts.
摘要翻译：本文公开了由Stachybotrys品种天然产生的新型酚氧化酶，其在碱性范围内具有最佳pH，可用于改变与染料和有色化合物相关的颜色以及抗染料转移应用。本文还公开了Stachybotrys属（Stachybotrys parvispora MUCL 38996）和Stachybotrys chartarum MUCL 38898属的能够天然产生新型酚氧化酶的菌株的生物纯培养物。本文公开了Stachybotrys酚氧化酶的氨基酸和核酸序列以及包含该核酸的表达载体和宿主细胞。本文公开了生产酚氧化酶的方法以及构建表达宿主的方法。

60. 发明授权

US06632930B2 Method of increasing recovery of heterologous active enzymes produced in plants 有权
标题翻译：提高植物中异源活性酶回收率的方法
公开(公告)号：US06632930B2
公开(公告)日：2003-10-14
申请号：US09881165
申请日：2001-06-14
申请人： Elizabeth Hood , John A. Howard , Michele Bailey , Franciscus J. C. van Gastel , Michael Ward , Huaming Wang , Susan Woodard
发明人： Elizabeth Hood , John A. Howard , Michele Bailey , Franciscus J. C. van Gastel , Michael Ward , Huaming Wang , Susan Woodard
IPC分类号： A23J100
CPC分类号： C12Y110/03002 , C12N9/0061 , C12N9/16 , C12N9/96 , C12N15/8257
摘要： A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.
摘要翻译：提供了增加植物中产生的活性酶的回收率的方法，其中酶需要用于活化的过渡金属辅因子。在植物发育，提取期间或提取后，将金属辅因子提供给酶。通过在非酶降解温度下温育提取的酶也可提供活性酶的回收。加入负离子盐进一步提高了活性酶的回收率。提供了使用铜溶液从植物中回收漆酶的最佳盐浓度

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