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51. 发明授权

US08030473B2 Method to trigger RNA interference 有权
标题翻译：触发RNA干扰的方法
公开(公告)号：US08030473B2
公开(公告)日：2011-10-04
申请号：US11334776
申请日：2006-01-06
申请人： James C. Carrington , Edwards Allen
发明人： James C. Carrington , Edwards Allen
IPC分类号： C12N15/113 , C12N15/63 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/111 , C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8274 , C12N15/8281 , C12N15/8283 , C12N15/8285 , C12N2310/14 , C12N2320/11
摘要： A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double-stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.
摘要翻译：描述了在体内产生siRNA的方法，以及在该方法中有用的构建体和组合物。该方法不依赖于使用含有反向重复或双重启动子的DNA或合成构建体，以形成完整或大部分双链RNA。相反，该方法取决于产生单链RNA转录物的构建体，并利用内源或体内产生的miRNA或siRNA来引发siRNA的产生。 miRNA或siRNA指导转录物的切割，并设置用于生产与构建体内的起始切割位点相邻编码的siRNA（通常长度为21个核苷酸）的寄存器。该方法导致相对于起始切割位点具有可预测大小和寄生（相）的siRNA的特异性形成。该方法可用于在体内产生特异性siRNA以灭活或抑制一种或多种靶基因或其他实体，例如病原体。

52. 发明授权

US07786350B2 Recombinant DNA constructs compressing engineered plant microRNAs and methods for controlling gene expression 有权
标题翻译：包含工程化植物微小RNA的重组DNA构建体和用于控制基因表达的方法
公开(公告)号：US07786350B2
公开(公告)日：2010-08-31
申请号：US11545099
申请日：2006-10-10
申请人： Edwards Allen , Larry A. Gilbertson , Shihshieh Huang , Elysia K. Krieger
发明人： Edwards Allen , Larry A. Gilbertson , Shihshieh Huang , Elysia K. Krieger
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides constructs containing engineered miRNAs or miRNA precursors derived from a maize miR408b, transgenic plant cells, plants, and seeds containing such constructs, and methods for their use.
摘要翻译：本发明提供了包含衍生自玉米miR408b，转基因植物细胞，植物和含有此类构建体的种子的工程化miRNA或miRNA前体的构建体及其使用方法。

53. 发明申请

US20070199095A1 Methods for producing hybrid seed 审中-公开
标题翻译：杂交种子的生产方法
公开(公告)号：US20070199095A1
公开(公告)日：2007-08-23
申请号：US11524564
申请日：2006-09-20
申请人： Edwards Allen , Larry Gilbertson , Nancy Houmard , Shihshieh Huang , Sergey Ivashuta , James Roberts
发明人： Edwards Allen , Larry Gilbertson , Nancy Houmard , Shihshieh Huang , Sergey Ivashuta , James Roberts
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8218 , A01H1/02 , A01H5/00 , A01H5/10 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
摘要翻译：本发明提供了生产非天然杂种种子的方法。还公开了可用于在作物植物上赋予诱导型不育性的特异性miRNA和miRNA识别位点，以及包含这种外源miRNA识别位点的重组DNA构建体。

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