专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明授权

US4174384A Fluorescence quenching with immunological pairs in immunoassays 失效
标题翻译：荧光猝灭与免疫对免疫测定
公开(公告)号：US4174384A
公开(公告)日：1979-11-13
申请号：US731255
申请日：1976-10-12
申请人： Edwin F. Ullman , Moshe Schwarzberg
发明人： Edwin F. Ullman , Moshe Schwarzberg
IPC分类号： G01N33/542 , G01N21/38 , C07G7/00 , G01N31/00 , G01N33/16
CPC分类号： G01N33/542 , Y10S435/968 , Y10S436/80 , Y10S436/816 , Y10S530/806
摘要： Immunoassays are provided employing antibodies and a fluorescer-quencher (F-Q) chromophoric pair, wherein one or both of the chromophoric pair are bonded to antibodies. Depending on the particular ligand of interest, various reagent combinations can be employed, where the amount of quenching is directly related to the amount of ligand present in the assay medium.In carrying out the assay, the unknown and antibody specific for the ligand of interest to which is bound one of the F-Q pair, are combined in an aqueous buffered medium. Depending on the protocol, different assay reagents are employed in the aqueous buffered medium: (1) ligand analog bonded to the other of the F-Q pair; (2) antibodies specific for the ligand to which is bound the other of the F-Q pair or; finally, (3) a combination of a plurality of ligands bonded together through linking groups to a hub molecule, usually a polymer, in combination with antibody bound to the other of the F-Q pair. The composition is irradiated with light at a wavelength, absorbed by the fluorescing molecule and the amount of fluorescence determined. By employing appropriate standards, the presence and amount of the ligand can be determined.
摘要翻译：使用抗体和荧光猝灭剂（F-Q）发色对提供免疫测定，其中发色对中的一个或两个与抗体结合。根据感兴趣的具体配体，可以使用各种试剂组合，其中猝灭量与测定培养基中存在的配体的量直接相关。在进行测定时，将结合F-Q对之一的目的配体的未知和抗体结合在含水缓冲介质中。根据方案，在水性缓冲介质中使用不同的测定试剂：（1）与F-Q对中的另一个键合的配体类似物; （2）对与F-Q对中的另一个结合的配体特异的抗体; 最后，（3）通过连接基团连接在一起的多个配体与轮毂分子（通常为聚合物）结合的F-Q对另一个结合的抗体的组合。用波长的光照射组合物，由荧光分子吸收并测定荧光量。通过采用适当的标准，可以确定配体的存在和量。

52. 发明授权

US4039385A Cardiac glycoside enzyme conjugates 失效
标题翻译：心脏糖苷酶缀合物
公开(公告)号：US4039385A
公开(公告)日：1977-08-02
申请号：US649942
申请日：1976-01-19
申请人： Edwin F. Ullman , Kenneth E. Rubenstein
发明人： Edwin F. Ullman , Kenneth E. Rubenstein
IPC分类号： C07J19/00 , C07J41/00 , C07J43/00 , G01N33/535 , G01N33/94 , C07G7/02
CPC分类号： C07J43/003 , C07J19/00 , C07J41/0016 , G01N33/535 , G01N33/946 , G01N33/948 , G01N33/9486
摘要： Enzyme conjugates are prepared of cardiac glycosides and aglycones for use in homogeneous enzyme immunoassays. The conjugates retain a substantial degree of the original activity of the enzyme and upon binding of receptor to the steroid moiety, a substantial diminution of enzyme activity is obtained. Various linking groups are employed for linking the steroid portion of the molecule to the enzyme, such as non-oxo carbonyl groups.
摘要翻译：制备用于均相酶免疫测定的强心苷和糖苷配基的酶缀合物。缀合物保留酶的原始活性的相当程度，并且当受体与类固醇部分结合时，获得了酶活性的显着降低。使用各种连接基团将分子的类固醇部分与酶例如非羰基羰基连接。

53. 发明授权

US3996345A Fluorescence quenching with immunological pairs in immunoassays 失效
标题翻译：荧光猝灭与免疫对免疫测定
公开(公告)号：US3996345A
公开(公告)日：1976-12-07
申请号：US591386
申请日：1975-06-30
申请人： Edwin F. Ullman , Moshe Schwarzberg
发明人： Edwin F. Ullman , Moshe Schwarzberg
IPC分类号： G01N33/18 , G01N33/542 , G01N21/00 , G01N33/16
CPC分类号： G01N33/542 , G01N33/1833 , Y10S435/968 , Y10S436/80 , Y10S436/815 , Y10S436/816 , Y10S436/817
摘要： Immunoassays employing antibodies and a fluorescer-quencher (F-Q) chromophoric pair, wherein one or both of the chromophoric pair are bonded to antibodies. Depending on the particular ligand of interest, various reagent combinations can be employed, where the amount of quenching is directly related to the amount of ligand present in the assay medium. In carrying out the assay, the unknown and antibody specific for the ligand of interest to which is bound one of the F-Q pair, are combined in an aqueous buffered medium. Depending on the protocol, different assay reagents are employed in the aqueous buffered medium: (1) ligand analog bonded to the other of the F-Q pair; (2) antibodies specific for the ligand to which is bound the other of the F-Q pair or; finally, (3) a combination of a plurality of ligands bonded together through linking groups to a hub molecule, usually a polymer, in combination with antibody bound to the other of the F-Q pair. The composition is irradiated with light at a wavelength, absorbed by the fluorescing molecule and the amount of fluorescence determined. By employing appropriate standards, the presence and amount of the ligand can be determined.
摘要翻译：使用抗体和荧光猝灭剂（F-Q）发色对的免疫测定法，其中发色对中的一个或两个与抗体结合。根据感兴趣的具体配体，可以使用各种试剂组合，其中猝灭量与测定培养基中存在的配体的量直接相关。在进行测定时，将结合F-Q对之一的目的配体的未知和抗体结合在含水缓冲介质中。根据方案，在水性缓冲介质中使用不同的测定试剂：（1）与F-Q对中的另一个键合的配体类似物; （2）对与F-Q对中的另一个结合的配体特异的抗体; 最后，（3）通过连接基团连接在一起的多个配体与轮毂分子（通常为聚合物）结合的F-Q对另一个结合的抗体的组合。用波长的光照射组合物，由荧光分子吸收并测定荧光量。通过采用适当的标准，可以确定配体的存在和量。

54. 发明授权

US06632606B1 Methods for single nucleotide polymorphism detection 失效
标题翻译：单核苷酸多态性检测方法
公开(公告)号：US06632606B1
公开(公告)日：2003-10-14
申请号：US09592053
申请日：2000-06-12
申请人： Edwin F. Ullman , Sharat Singh
发明人： Edwin F. Ullman , Sharat Singh
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q2565/519 , C12Q2535/125 , C12Q2563/179 , C12Q2563/131
摘要： Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target DNA where the next succeeding 3′-nucleotide is a potential single nucleotide polymorphism and coding composition members, where the members are unique for each primer, and (2) differentially labeled terminating nucleotides, where the label permits separation of the terminating nucleotides. Desirably the particles are separated into groups having a common prevalent next succeeding nucleotide. The particles and target DNA are combined under nucleotide extending conditions, the particles separated into groups in accordance with the terminating nucleotide and the coding members identified, so that one knows the sequence and the single nucleotide polymorphism. Various protocols are provided for the determination.
摘要翻译：提供了用于使用具有（1）与目标DNA中的序列互补的引物，其中下一个后续的3'-核苷酸是潜在的单核苷酸多态性和编码组成成员的粒子来确定靶DNA中大量单核苷酸多态性的方法和组合物，其中成员对于每个引物是独特的，和（2）差异标记的终止核苷酸，其中标记允许分离终止的核苷酸。理想地，将颗粒分离成具有普通的下一个后续核苷酸的基团。颗粒和靶DNA在核苷酸延伸条件下合并，根据终止的核苷酸和鉴定的编码成员将颗粒分成组，以便知道序列和单核苷酸多态性。提供各种协议用于确定。

55. 发明授权

US06406913B1 Assay method utilizing induced luminescence 失效
标题翻译：利用诱导发光的测定方法
公开(公告)号：US06406913B1
公开(公告)日：2002-06-18
申请号：US08471130
申请日：1995-06-06
申请人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
发明人： Edwin F. Ullman , Hrair Kirakossian , John S. Pease , Yuri Daniloff , Daniel B. Wagner
IPC分类号： G01T1161
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中的分析物的方法。一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。活化的化学发光化合物随后产生光。所产生的光量与介质中分析物的量有关。优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。还公开了组合物和试剂盒。

56. 发明授权

US06326159B1 Receptors for immune complexes 失效
标题翻译：免疫复合物受体
公开(公告)号：US06326159B1
公开(公告)日：2001-12-04
申请号：US08300572
申请日：1994-09-02
申请人： Edwin F. Ullman , John Jelesko , Marcel R. Pirio , Thomas D. Kempe
发明人： Edwin F. Ullman , John Jelesko , Marcel R. Pirio , Thomas D. Kempe
IPC分类号： C07K1600
CPC分类号： C07K16/42 , G01N33/564 , G01N33/948 , Y10S435/975 , Y10S436/80 , Y10S436/804 , Y10S436/805 , Y10S436/808 , Y10S436/815 , Y10S436/817 , Y10S530/808
摘要： Receptors are disclosed that are antibodies that exhibit a binding affinity for an immune complex of a monoepitopic antigen and an antibody for such antigen that is substantially greater than the binding affinity for the monoepitopic antigen or the antibody for the monoepitopic antigen apart from the immune complex. Normally, the monoepitopic antigen has a molecular weight less than 1500 and is an organic compound. The antibodies of the present invention find use in a method for determining a monoepitopic antigen in a sample suspected of containing such antigen. The method comprises forming an immune sandwich complex comprising the monoepitopic antigen or an analog thereof, a first monoclonal antibody that binds to the monoepitopic antigen, and a second monoclonal antibody that is an antibody of the present invention and detecting the immune sandwich complex. Compositions of matter and kits for use in conducting an assay in accordance with the invention as well as methods for producing the above receptors are also disclosed.
摘要翻译：公开了受体，其是对单表皮抗原和这种抗原的抗体的免疫复合物具有结合亲和力的抗体，其显着大于除了免疫复合物之外的单表位抗原或单表位抗原的抗体的结合亲和力。通常，单表位抗原的分子量小于1500，是有机化合物。本发明的抗体用于确定疑似含有该抗原的样品中的单表位抗原的方法。该方法包括形成包含单表皮抗原或其类似物的免疫三明治复合物，与单表位抗原结合的第一单克隆抗体和作为本发明抗体的第二单克隆抗体，并检测免疫三明治复合物。还公开了用于进行根据本发明的测定的物质和试剂盒的组合物以及用于产生上述受体的方法。

57. 发明授权

US06284546B1 Method and device for photodetection 失效
标题翻译：光电检测方法及装置
公开(公告)号：US06284546B1
公开(公告)日：2001-09-04
申请号：US08469578
申请日：1995-06-06
申请人： Zbigniew T. Bryning , Benjamin R. Irvin , Hrair Kirakossian , Edwin F. Ullman
发明人： Zbigniew T. Bryning , Benjamin R. Irvin , Hrair Kirakossian , Edwin F. Ullman
IPC分类号： G01N2176
CPC分类号： G01N21/8806 , B01F5/0085 , B01F11/00 , B01F13/0001 , B01F13/0013 , B01F13/0809 , B01F15/0201 , B01F2215/0037 , B01L3/5085 , B01L2400/0415 , B01L2400/0433 , G01N21/8483 , G01N2001/386 , G01N2035/00237 , G01N2035/00554 , Y10T436/110833 , Y10T436/25
摘要： The present invention provides methods and apparatus for mixing two or more liquids and detecting light emitted by the mixture. The method comprises forming a liquid droplet containing two or more liquids on a substantially planar surface in containerless containment on the surface and causing the droplet to deform in an essentially zero air flow environment thereby mixing the liquids and surrounding the planar surface with a reflective housing and photodetector. An apparatus of the invention comprises (a) a substantially planar support, (b) means for dispensing liquids onto the support to form a droplet, and (c) non-evaporative means for causing the droplet to deform without deforming the support thereby mixing the liquids and a two part reflective housing and photodetector which is moveable. The drop can be deformed by, for example, application of acoustic energy or a variable electrostatic field. The methods and apparatus have particular application to the determination of an analyte.
摘要翻译：本发明提供了混合两种或多种液体并检测由混合物发射的光的方法和装置。该方法包括在表面上的无容器容纳物中的基本上平坦的表面上形成含有两种或更多种液体的液滴，并使液滴在基本为零的空气流动环境中变形，从而混合液体并围绕平面表面与反射壳体光电探测器本发明的装置包括（a）基本上平面的支撑件，（b）用于将液体分配到支撑件上以形成液滴的装置，和（c）非蒸发装置，用于使液滴变形而不使支架变形，液体和可移动的两部分反射壳体和光电检测器。可以通过例如施加声能或可变静电场来使液滴变形。所述方法和装置特别适用于分析物的测定。

58. 发明授权

US06180354B2 Metal chelate containing compositions for use in chemiluminescent assays 失效
标题翻译：含金属螯合物的组合物用于化学发光测定
公开(公告)号：US06180354B2
公开(公告)日：2001-01-30
申请号：US08480430
申请日：1995-06-06
申请人： Sharat Singh , Edwin F. Ullman
发明人： Sharat Singh , Edwin F. Ullman
IPC分类号： G01N3353
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , G01N33/533 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/968 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T436/13
摘要： Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.
摘要翻译：公开了包含（a）金属螯合物的组合物，其中金属选自铕，铽，镝，钐锇和钌至少六配位状态，和（b）具有被两个芳基取代的双键的化合物基团，氧原子和选自氧，硫和氮的原子，其中一个芳基相对于另一个赋予电子。这种组合物优选掺入胶乳颗粒材料中。还公开了用于测定怀疑含有分析物的培养基中的分析物的方法和试剂盒。所述方法和试剂盒采用如上所述的组合物作为一种组分。

59. 发明授权

US6121001A Detection of nucleic acids by target-catalyzed product formation 有权
标题翻译：通过靶催化产物形成检测核酸
公开(公告)号：US6121001A
公开(公告)日：2000-09-19
申请号：US440363
申请日：1999-11-15
申请人： Linda M. Western , Samuel J. Rose , Edwin F. Ullman
发明人： Linda M. Western , Samuel J. Rose , Edwin F. Ullman
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/44
CPC分类号： C12Q1/689 , C12Q1/682 , C12Q1/6823
摘要： A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5'-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5'-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3' of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.
摘要翻译：公开了一种修饰寡核苷酸的方法，该方法可用于检测多核苷酸分析物。在等温条件下，在5'-核酸酶的存在下，寡核苷酸与多核苷酸例如多核苷酸分析物可逆地杂交。多核苷酸分析物用作识别元件，使得5'-核酸酶能够切割寡核苷酸，以提供（i）与多核苷酸分析物基本上不可杂交的第一片段和（ii）位于3'末端的第3个片段第一片段（在完整寡核苷酸中）并且与多核苷酸分析物基本上可杂交。相对于多核苷酸分析物的摩尔量，获得至少100倍摩尔过量的第一片段和/或第二片段。检测到第一片段和/或第二片段的存在，其存在表明多核苷酸分析物的存在。该方法特别适用于多核苷酸分析物如DNA的检测。还公开了用于根据本发明的方法的套件。

60. 发明授权

US6110677A Oligonucleotide modification, signal amplification, and nucleic acid detection by target-catalyzed product formation 失效
标题翻译：通过靶催化产物形成的寡核苷酸修饰，信号扩增和核酸检测
公开(公告)号：US6110677A
公开(公告)日：2000-08-29
申请号：US15949
申请日：1998-01-30
申请人： Linda M. Western , Samuel J. Rose , Edwin F. Ullman
发明人： Linda M. Western , Samuel J. Rose , Edwin F. Ullman
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/44 , C12Q1/48
CPC分类号： C12Q1/689 , C12Q1/682 , C12Q1/6823
摘要： A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5'-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5'-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3' of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.
摘要翻译：公开了一种修饰寡核苷酸的方法，该方法可用于检测多核苷酸分析物。在等温条件下，在5'-核酸酶的存在下，寡核苷酸与多核苷酸例如多核苷酸分析物可逆地杂交。多核苷酸分析物用作识别元件，使得5'-核酸酶能够切割寡核苷酸，以提供（i）与多核苷酸分析物基本上不可杂交的第一片段和（ii）位于3'末端的第3个片段第一片段（在完整寡核苷酸中）并且与多核苷酸分析物基本上可杂交。相对于多核苷酸分析物的摩尔量，获得至少100倍摩尔过量的第一片段和/或第二片段。检测到第一片段和/或第二片段的存在，其存在表明多核苷酸分析物的存在。该方法特别适用于多核苷酸分析物如DNA的检测。还公开了用于根据本发明的方法的套件。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式