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51. 发明授权

US06939702B1 Lipase variant 有权
标题翻译：脂肪酶变体
公开(公告)号：US06939702B1
公开(公告)日：2005-09-06
申请号：US09937919
申请日：2000-03-30
申请人： Jesper Vind , Allan Svendsen , Shamkant Anant Patkar , Kim Vilbour Andersen , Dorte Aaby Halkier , Kirsten Bojsen
发明人： Jesper Vind , Allan Svendsen , Shamkant Anant Patkar , Kim Vilbour Andersen , Dorte Aaby Halkier , Kirsten Bojsen
IPC分类号： C12N15/09 , C07H21/04 , C11D1/02 , C11D1/66 , C11D3/386 , C11D10/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/20
CPC分类号： C11D3/38627 , C12N9/20
摘要： Certain variants of Lipolase (wild-type Humicolalanuginosa lipase) have a particularly good first-wash performance in a detergent solution. The variants should comprise one or more substitutions with positive amino acids near the N-terminal in the three-dimensional structure. The variants should further comprise a peptide addition at the C-terminal and/or should meet certain limitations on electrically charged amino acids at positions 90-101 and 210.
摘要翻译： Lipolase（野生型Humicolalanuginosa脂肪酶）的某些变体在洗涤剂溶液中具有特别好的第一洗涤性能。变体应包含一个或多个在三维结构中N-末端附近具有阳性氨基酸的取代。变体应进一步包含C末端的肽加成和/或应满足90-101和210位带电氨基酸的某些限制。

52. 发明授权

US06783941B2 Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes 失效
标题翻译：通过异源双链体的错配修复在体外制备多核苷酸文库的方法
公开(公告)号：US06783941B2
公开(公告)日：2004-08-31
申请号：US10004993
申请日：2001-12-05
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C12Q168
CPC分类号： C12N15/1027
摘要： A method for making polynucleotide libraries that comprise new recombined polynucleotides by using mismatch repair enzymes or proteins to shuffle at least two variants of the same polynucleotide or at least two homologous polynucleotides through a Recombinatorial Chain Reaction, RCR.
摘要翻译：通过使用错配修复酶或蛋白质来制备包含新的重组多核苷酸的多核苷酸文库的方法，其通过重组体链式反应RCR混洗相同多核苷酸的至少两种变体或至少两种同源多核苷酸。

53. 发明授权

US5817495A H.sub.2 O.sub.2 -stable peroxidase variants 失效
标题翻译： H2O2稳定的过氧化物酶变体
公开(公告)号：US5817495A
公开(公告)日：1998-10-06
申请号：US624545
申请日：1996-05-07
申请人： Anders Hjelholt Pedersen , Jesper Vind , Allan Svendsen , Joel R. Cherry , Michael Lamsa , Palle Schneider , Birger Rostgaard Jensen
发明人： Anders Hjelholt Pedersen , Jesper Vind , Allan Svendsen , Joel R. Cherry , Michael Lamsa , Palle Schneider , Birger Rostgaard Jensen
IPC分类号： C12N15/09 , C11D3/386 , C11D3/39 , C12N9/08 , C12R1/685 , C12R1/69 , C11D7/42 , C11D7/54
CPC分类号： C12N9/0065 , C11D3/38654 , C11D3/3947
摘要： The present invention relates to a novel variant of peroxidase with improved stability at alkaline conditions, and a bleaching or detergent composition comprising the peroxidase variant.
摘要翻译： PCT No.PCT / DK94 / 00382 Sec。 371日期：1996年5月7日 102（e）日期1996年5月7日PCT提交1994年10月13日PCT公布。出版物WO95 / 10602 日期1995年4月20日本发明涉及在碱性条件下具有改善的稳定性的过氧化物酶的新型变体，以及包含过氧化物酶变体的漂白或洗涤剂组合物。

54. 发明授权

US08298800B2 Lipolytic enzyme variants 有权
标题翻译：脂解酶变体
公开(公告)号：US08298800B2
公开(公告)日：2012-10-30
申请号：US12612398
申请日：2009-11-04
申请人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Thea Jorgensen
发明人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Thea Jorgensen
IPC分类号： C12N9/16 , A21D8/02
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译：本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。多肽可以特别地对极性脂质具有活性。

55. 发明授权

US08187854B2 Lipase variants 有权
标题翻译：脂肪酶变体
公开(公告)号：US08187854B2
公开(公告)日：2012-05-29
申请号：US12161582
申请日：2007-01-22
申请人： Jesper Vind , Jürgen Carsten Franz Knötzel , Kim Borch , Allan Svendsen , Thomas Honger Callisen , Debbie Yaver , Mads Eskelund Bjornvad , Peter Kamp Hansen , Haiyan Ge , Michael Lamsa
发明人： Jesper Vind , Jürgen Carsten Franz Knötzel , Kim Borch , Allan Svendsen , Thomas Honger Callisen , Debbie Yaver , Mads Eskelund Bjornvad , Peter Kamp Hansen , Haiyan Ge , Michael Lamsa
IPC分类号： C12N9/20 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/20 , C12N9/18
摘要： The invention provides variant lipases, preferably, variants with reduced tendency to odor generation obtained by introducing mutations in one or more regions identified in a parent lipase.
摘要翻译：本发明提供了变体脂肪酶，优选具有通过在母体脂肪酶中鉴定的一个或多个区域中引入突变获得的具有降低的气味产生倾向的变体。

56. 发明申请

US20120088277A1 Glucoamylase Variants 失效
公开(公告)号：US20120088277A1
公开(公告)日：2012-04-12
申请号：US13329550
申请日：2011-12-19
申请人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
发明人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
IPC分类号： C12P19/14 , C12N1/19 , C12N15/80 , C12N9/34 , C12N15/56
CPC分类号： C12N9/2428 , C12P19/14 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.

57. 发明申请

US20110165655A1 LIPOLYTIC ENZYMES 审中-公开
标题翻译：脂肪酶
公开(公告)号：US20110165655A1
公开(公告)日：2011-07-07
申请号：US12826960
申请日：2010-06-30
申请人： ALLAN SVENDSEN , JESPER VIND , HANS PETER HELDT-HANSEN , LUISE ERLANDSEN
发明人： ALLAN SVENDSEN , JESPER VIND , HANS PETER HELDT-HANSEN , LUISE ERLANDSEN
IPC分类号： C12N9/20
CPC分类号： C12N9/20 , C12Y301/01003
摘要： The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.
摘要翻译：本发明人开发了一种使用蛋白质工程的方法来生产对于具有两个亲脂基团的两亲底物中的一个酯键具有相对较高活性的脂肪分解酶），并且在具有一个亲油基团的两亲底物中具有较低的酯键活性。相对较高的磷脂酶活性和较低的溶血磷脂酶活性。

58. 发明授权

US07919298B2 Polypeptides having lipase activity and polynucleotides encoding same 有权
标题翻译：具有脂肪酶活性的多肽和编码该多肽的多核苷酸
公开(公告)号：US07919298B2
公开(公告)日：2011-04-05
申请号：US12393283
申请日：2009-02-26
申请人： Jesper Vind , Jurgen Carsten Franz Knotzel , Kim Borch , Allan Svendsen , Thomas Hoenger Callisen , Debbie Yaver , Mads Eskelund Bjoernvad , Peter Kamp Hansen
发明人： Jesper Vind , Jurgen Carsten Franz Knotzel , Kim Borch , Allan Svendsen , Thomas Hoenger Callisen , Debbie Yaver , Mads Eskelund Bjoernvad , Peter Kamp Hansen
IPC分类号： C12N9/20 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/68 , C12P21/04 , C12P7/00 , C07H21/04
CPC分类号： C12N9/20
摘要： The invention provides polypeptides obtained by introducing mutations in one or more regions identified in a parent lipase. The polypeptides of the present invention have surprisingly been found to have a low specific activity towards short chain fatty acids leading to a reduced odor generation and an increased BR over the lipases known in the art.
摘要翻译：本发明提供通过在母体脂肪酶中鉴定的一个或多个区域中引入突变获得的多肽。令人惊奇地发现本发明的多肽对短链脂肪酸具有低比活性，导致气味产生减少，并且在本领域已知的脂肪酶上增加BR。

59. 发明授权

US07833772B2 Glucoamylase variants 失效
标题翻译：葡糖淀粉酶变体
公开(公告)号：US07833772B2
公开(公告)日：2010-11-16
申请号：US11524693
申请日：2006-09-21
申请人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
发明人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
IPC分类号： C12N9/34 , C12N9/30 , C12N9/24 , C12N9/00 , C12P19/20 , C12C1/00 , C07H21/04
CPC分类号： C12N9/2428 , C12P19/14 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.
摘要翻译：本发明涉及亲本真菌葡糖淀粉酶的变体，其使用糖基底显示出改善的热稳定性和/或增加的比活性。

60. 发明申请

US20100221783A1 Expression Cloning Method Suitable for Selecting Library Clones Producing a Polypeptide of Interest 审中-公开
标题翻译：表达克隆方法适用于选择产生多肽兴趣的文库克隆
公开(公告)号：US20100221783A1
公开(公告)日：2010-09-02
申请号：US12599384
申请日：2008-05-07
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C12P21/04
CPC分类号： C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a recombinant polypeptide of interest, the method comprising the steps of: a) providing a polynucleotide library encoding one or more polypeptides of interest, wherein the library was prepared in an expression cloning vector comprising at least the following elements: i) a polynucleotide encoding a selectable marker; ii) a fungal replication initiation sequence, preferably an autonomously replicating sequence (ARS); and iii) a polynucleotide comprising in sequential order: a promoter derived from a fungal cell, a cloning-site into which the library is cloned, and a transcription terminator; b) transforming a mutant of a parent filamentous fungal host cell with the library, wherein the frequency of non-homologous recombination in the mutant has been decreased compared to the parent; c) culturing the transformed host cell obtained in (b) under conditions suitable for expression of the polynucleotide library; and d) selecting a transformed host cell which produces the polypeptide of interest.
摘要翻译：本发明涉及用于产生感兴趣的重组多肽的方法，所述方法包括以下步骤：a）提供编码一种或多种感兴趣多肽的多核苷酸文库，其中所述文库在包含至少以下的表达克隆载体中制备元素：i）编码可选择标记的多核苷酸; ii）真菌复制起始序列，优选自主复制序列（ARS）; 和iii）包含序列顺序的多核苷酸：源自真菌细胞的启动子，克隆文库的克隆位点和转录终止子; b）用文库转化亲本丝状真菌宿主细胞的突变体，其中与亲本相比，突变体中非同源重组的频率已经降低; c）在适于表达多核苷酸文库的条件下培养（b）中获得的转化的宿主细胞; 和d）选择产生感兴趣多肽的转化的宿主细胞。

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