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41. 发明申请

US20050049313A1 Electron mediator, electron mediator immobilized electrode, and biofuel cell using the electrode 有权
标题翻译：电子介体，电子介体固定电极和使用电极的生物燃料电池
公开(公告)号：US20050049313A1
公开(公告)日：2005-03-03
申请号：US10807489
申请日：2004-03-23
申请人： Matsuhiko Nishizawa , Tomokazu Matsue , Jun-ichi Kosuge , Noboru Fukasaku
发明人： Matsuhiko Nishizawa , Tomokazu Matsue , Jun-ichi Kosuge , Noboru Fukasaku
IPC分类号： C12M1/34 , C07C66/02 , C07C235/78 , H01M8/16 , A61K31/12
CPC分类号： C07C66/02 , C07C235/78 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An object of the present invention is to provide a highly functional mediator capable of smoothly proceeding electron transfer between an electrode and a biocatalyst based on the premise that the mediator excels in safety and cost and to provide an electrode and a cell with sufficient amount of such a mediator molecule immobilized efficiently and strongly. The present invention provides a mediator mediating electron transfer between an enzyme and an electrode and containing a quinone molecule derivative, in particular, a mediator containing a VK3 derivative. In addition, the present invention provides an electrode and a biofuel cell to which those mediators are applied.
摘要翻译：本发明的目的是提供一种能够平稳地进行电极和生物催化剂之间的电子转移的高功能介体，其基于这样的前提，即介质优良的安全性和成本，并且提供具有足够量的这样的电极和电池一种有效强固地固定的介体分子。本发明提供介导酶和电极之间的电子转移并含有醌分子衍生物，特别是含有VK3衍生物的介体的介体。此外，本发明提供一种电极和生物燃料电池，其中应用这些介体。

42. 发明申请

US20040219623A1 Method for quantitatively determining LDL cholesterols 审中-公开
标题翻译：定量测定LDL胆固醇的方法
公开(公告)号：US20040219623A1
公开(公告)日：2004-11-04
申请号：US10859999
申请日：2004-06-04
申请人： DAIICHI PURE CHEMICALS CO., LTD.
发明人： Mitsuhiro Nakamura , Kazuo Nakanishi , Koichi Hino , Mitsuhisa Manabe
IPC分类号： C12Q001/60
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

43. 发明申请

US20030162710A1 Physiologically active polypeptide and DNA 审中-公开
公开(公告)号：US20030162710A1
公开(公告)日：2003-08-28
申请号：US10335847
申请日：2003-01-03
申请人： DAIICHI PURE CHEMICALS CO., LTD.
发明人： Tetsuji Sudoh , Keiji Maekawa , Naoto Minamino , Kenji Kangawa , Hisayuki Matsuo , Atsushi Izumi , Mika Takashima
IPC分类号： A61K038/16 , C07K014/47 , C07H021/04
CPC分类号： C07K14/58 , A61K38/00
摘要： A physiologically active polypeptide derived from human brain and a DNA fragment comprising the base sequence encoding the polypeptide are disclosed. The polypeptide possesses excellent smooth muscle relaxation activity, diuretic or natriuretic activity, and vasodepressor activity, and is thus useful as a medicine for curing circulation diseases, e.g. cardiac edema, nephric edema, hepatic edema, pulmonary edema, hypertension, congestive heat failure, and acute and chronic renal failure.

44. 发明申请

US20020086843A1 Physiologically active polypeptide and DNA 审中-公开
标题翻译：生理活性多肽和DNA
公开(公告)号：US20020086843A1
公开(公告)日：2002-07-04
申请号：US09902161
申请日：2001-07-11
申请人： Daiichi Pure Chemicals Co., Ltd.
发明人： Tetsuji Sudoh , Keiji Maekawa , Naoto Minamino , Kenji Kangawa , Hisayuki Matsuo , Atsushi Izumi , Mika Takashima
IPC分类号： A61K048/00 , C12N009/00 , C07H021/04
CPC分类号： C07K14/58 , A61K38/00
摘要： A physiologically active polypeptide derived from human brain and a DNA fragment comprising the base sequence encoding the polypeptide are disclosed. The polypeptide possesses excellent smooth muscle relaxation activity, diuretic or natriuretic activity, and vasodepressor activity, and is thus useful as a medicine for curing circulation diseases, e.g. cardiac edema, nephric edema, hepatic edema, pulmonary edema, hypertension, congestive heat failure, and acute and chronic renal failure.
摘要翻译：公开了衍生自人脑的生理活性多肽和包含编码多肽的碱基序列的DNA片段。该多肽具有优异的平滑肌松弛活性，利尿或利尿钠活性和血管舒张活性，因此可用作治疗循环疾病的药物，例如，心脏水肿，肾水肿，肝水肿，肺水肿，高血压，充血性热衰竭以及急性和慢性肾衰竭。

45. 发明授权

US5871949A Method of quantitative assay for 1,5-anhydroglucitol and reagent for quantitative assay 失效
标题翻译：定量测定1,5-脱水葡萄糖醇和试剂的方法
公开(公告)号：US5871949A
公开(公告)日：1999-02-16
申请号：US980792
申请日：1997-12-01
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
IPC分类号： C12Q1/32 , C12Q1/00 , C12Q1/54
CPC分类号： C12Q1/32
摘要： Using a 1,5-anhydroglucitol dehydrogenase capable of acting on 1,5-anhydroglucitol and directly catalyzing a reducing chromophoric agent in the absence of an electron carrier, the 1,5-anhydroglucitol dehydrogenase is allowed to act on 1,5-anhydroglucitol in the presence of the reducing chromophoric agent preferably after the glucose in the specimen has been changed, or while being changed, into such a structure that it does not react with the 1,5-anhydroglucitol dehydrogenase in the specimen, by the aid of a glucose eliminator. Then, the amount of the resultant reduced colored substance is measured. As the 1,5-anhydroglucitol dehydrogenase, an enzyme produced by a microorganism having the ability to produce the 1,5-anhydroglucitol dehydrogenase is preferably used.
摘要翻译：使用能够作用于1,5-脱水葡萄糖醇并在不存在电子载体的情况下直接催化还原性发色剂的1,5-脱水葡萄糖醇脱氢酶，使1,5-脱水葡萄糖醇脱氢酶作用于1,5-脱水葡萄糖醇还原性发色剂的存在优选在样品中的葡萄糖已经被改变或改变之后，使其不与样品中的1,5-脱水葡萄糖醇脱氢酶反应，借助于葡萄糖消除者然后，测量所得的还原着色物质的量。作为1,5-脱水葡萄糖醇脱氢酶，优选使用具有生产1,5-脱水葡萄糖醇脱氢酶能力的微生物产生的酶。

46. 发明授权

US5773304A Method for quantitatively determining cholesterol 失效
标题翻译：定量测定胆固醇的方法
公开(公告)号：US5773304A
公开(公告)日：1998-06-30
申请号：US704681
申请日：1996-09-19
申请人： Koichi Hino , Mitsuhiro Nakamura , Mitsuhisa Manabe
发明人： Koichi Hino , Mitsuhiro Nakamura , Mitsuhisa Manabe
IPC分类号： G01N33/53 , C12Q1/26 , C12Q1/44 , C12Q1/60 , G01N33/92 , G01N33/533
CPC分类号： G01N33/92 , C12Q1/60 , Y10S435/962 , Y10S436/815 , Y10S436/824 , Y10S436/825 , Y10S436/826 , Y10T436/104165 , Y10T436/107497 , Y10T436/25
摘要： A method for quantitatively determining cholesterol in high density lipoproteins, in which, prior to the determination of cholesterol by an enzymatic method, a surfactant and a substance which forms a complex with lipoproteins other than high density lipoproteins are added to a sample containing lipoproteins. The method does not require any pretreatments such as centrifugal separation. With a simple operation, cholesterol in HDLs can be measured effectively. Also, this method can be adopted in a variety of automated analyzers, and thus is very useful in the field of clinical assays.
摘要翻译： PCT No.PCT / JP95 / 00641 Sec。 371日期1996年9月19日 102（e）1996年9月19日PCT PCT 1995年4月3日PCT公布。 WO96 / 23902 PCT公开号日期1996年8月8日一种用于定量测定高密度脂蛋白胆固醇的方法，其中在通过酶法测定胆固醇之前，将表面活性剂和与高密度脂蛋白以外的脂蛋白形成复合物的物质加入到样品含有脂蛋白。该方法不需要任何预处理，如离心分离。通过简单的操作，可以有效测量HDL中的胆固醇。此外，该方法可以在各种自动分析仪中使用，因此在临床测定领域非常有用。

47. 发明授权

US5635046A Method for drying polyacrylamide gel after electrophoresis 失效
标题翻译：电泳后聚丙烯酰胺凝胶干燥的方法
公开(公告)号：US5635046A
公开(公告)日：1997-06-03
申请号：US694459
申请日：1996-08-07
申请人： Kazuaki Notsu , Mieko Shiratori
发明人： Kazuaki Notsu , Mieko Shiratori
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44717 , G01N27/44721
摘要： A polyacrylamide gel which has undergone electrophoresis is dried in the presence of pressure applied via a semipermeable film placed on the gel. Before applying the semipermeable film onto the gel, solution components contained in the gel is replaced by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 or more carbon atoms, and water-soluble polymers. According to this method, a polyacrylamide gel which has undergone electrophoresis can be dried with ease and reduced costs while maintaining its transparency and without causing cracks even in the case of a gel containing polyacrylamide gel at a high concentration.
摘要翻译：已经经过电泳的聚丙烯酰胺凝胶在通过置于凝胶上的半透膜施加的压力存在下干燥。在将半透膜施加到凝胶上之前，由含有一种或多种选自糖类，具有4个或更多个碳原子的糖醇和水溶性聚合物的一种或多种物质的水溶液代替凝胶中所含的溶液成分。根据该方法，即使在含有高浓度聚丙烯酰胺凝胶的凝胶的情况下，经过电泳的聚丙烯酰胺凝胶也可以容易地进行干燥，降低成本，同时保持其透明性并且不引起裂纹。

48. 发明授权

US5494804A Differential determination process of .alpha.-amylase isoenzyme activities 失效
标题翻译： α-淀粉酶同工酶活性的差异测定方法
公开(公告)号：US5494804A
公开(公告)日：1996-02-27
申请号：US305917
申请日：1994-09-16
申请人： Riichiro Uchida , Shoichi Tokutake , Nobuyuki Yamaji , Yoshinori Motoyama , Kenji Hosoi
发明人： Riichiro Uchida , Shoichi Tokutake , Nobuyuki Yamaji , Yoshinori Motoyama , Kenji Hosoi
IPC分类号： C12N9/99 , C12Q1/00 , C12Q1/34 , C12Q1/40 , C07G3/00
CPC分类号： C12Q1/007
摘要： A process for differentially determining .alpha.-amylase isoenzyme activities by the inhibitor method, in which 6.sup.3 -deoxymaltotriose (DOG3) represented by the formula ##STR1## is used as an inhibitor.
摘要翻译：通过抑制剂方法差异测定α-淀粉酶同工酶活性的方法，其中由式（IMAGE）表示的63-脱氧麦芽三糖（DOG3）用作抑制剂。

49. 发明授权

US5306404A Process for preparing polyacrylamide gel plate for electrophoresis 失效
标题翻译：制备聚丙烯酰胺凝胶板电泳方法
公开(公告)号：US5306404A
公开(公告)日：1994-04-26
申请号：US009109
申请日：1993-01-26
申请人： Kazuaki Notsu , Nobuyoshi Ebata , Akiko Udagawa , Mieko Shiratori
发明人： Kazuaki Notsu , Nobuyoshi Ebata , Akiko Udagawa , Mieko Shiratori
IPC分类号： C08F20/56 , G01N27/447 , C25B7/00
CPC分类号： C08F20/56 , G01N27/44704 , G01N27/44747
摘要： A process for preparing a polyacrylamide gel plate for electrophoresis with any desired concentrations, which comprises mixing a high concentration acrylamide monomer solution, a low concentration peroxide solution, and a low concentration reducing solution at an arbitrary ratio, and introducing the mixture into a gel supporter. The process enables preparation of a large quantity of high quality polyacrylamide gel plates for electrophoresis which is useful for analysis of high molecular weight in vivo components typified by proteins with a good reproducibility. It also contributes to the production of various gels having a plurality of gel concentrations or having a specified concentration gradient, and serves for improving the productivity of multi-products manufacturing of such gel plates.
摘要翻译：一种用于制备任何所需浓度的用于电泳的聚丙烯酰胺凝胶板的方法，其包括以任意比例混合高浓度丙烯酰胺单体溶液，低浓度过氧化物溶液和低浓度还原溶液，并将混合物引入凝胶支持物。该方法能够制备大量用于电泳的高质量聚丙烯酰胺凝胶板，其可用于分析以具有良好重现性的蛋白质代表的高分子量体内成分。它还有助于生产具有多种凝胶浓度或具有特定浓度梯度的各种凝胶，并且用于提高这种凝胶板的多产品制造的生产率。

50. 发明授权

US5120641A Method for measurement of serotonin uptake sites and radioligand therefor 失效
标题翻译：用于测量丝氨酸摄取位点和其中的放射性质的方法
公开(公告)号：US5120641A
公开(公告)日：1992-06-09
申请号：US633676
申请日：1990-12-26
申请人： Kenji Hashimoto , Tsuyoshi Goromaru
发明人： Kenji Hashimoto , Tsuyoshi Goromaru
IPC分类号： G01N33/50 , G01N33/566 , G01N33/60 , G01N33/94
CPC分类号： G01N33/94 , G01N33/9453
摘要： Disclosed herein is an improved method for measurement of serotonin uptake sites in a sample, in which a radioligand is incubated with a sample and then the radioactivity of the radioligand bound to the sample is determined, wherein the improvement comprises using tritium-labeled 6-nitro-2-N-piperazinylquinoline or an acid addition salt thereof as the radioligand.

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