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    • 50. 发明授权
    • Method of transforming monocotyledons using scutella of immature embryos
    • 使用未成熟胚的小檗转化单子叶植物的方法
    • US07939328B1
    • 2011-05-10
    • US08428238
    • 1994-09-01
    • Hideaki SaitoYuji IshidaYukoh HieiToshihiko Komari
    • Hideaki SaitoYuji IshidaYukoh HieiToshihiko Komari
    • C12N15/82
    • C12N15/8205
    • Disclosed is a method of transforming monocotyledons which necessitates only a short period from the transformation to the regeneration of a whole plant as compared with the conventional methods, thus reducing the frequency of occurrence of mutants, and can be generally applied to the plants for which any system of regenerating the whole plants from protoplasts has not been established, and in which the material to be used can be readily prepared without any particular apparatuses. The present invention provides a method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genus Agrobacterium containing a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.
    • 本发明公开了一种转化单子叶植物的方法,其与常规方法相比仅需要从整个植物的转化到再生的短的时间,从而降低突变体的发生频率,并且通常可以应用于任何 尚未建立从原生质体再生整株植物的系统,其中待使用的材料可以在没有任何特定装置的情况下容易地制备。 本发明提供一种用于将单子叶植物的未成熟胚的鳞片状细菌与含有未成熟胚的未分化处理的所需基因的农杆菌属的农杆菌属转化成单转基因的方法,得到转化体。