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    • 47. 发明授权
    • Method of selecting a cardiomyoctye using intracellular mitochondria as an indicator
    • 使用细胞内线粒体作为指标选择心肌细胞的方法
    • US08623649B2
    • 2014-01-07
    • US11660581
    • 2005-08-26
    • Fumiyuki HattoriKeiichi Fukuda
    • Fumiyuki HattoriKeiichi Fukuda
    • A61K35/34
    • C12N5/0657C12N2506/02G01N33/56966
    • The invention relates to methods of selecting a cardiomyocyte from a cell population derived from a whole heart or a differentiated cell population derived from a stem cell without genetic alteration. Specifically, the invention relates to a method of selecting a cardiomyocyte from a cardiomyocyte-containing cell mixture without genetic alteration of a cardiomyocyte, on the basis of a relative content of cellular mitochondria and/or a relative mitochondrial transmembrane potential of the cell. The invention also relates to methods of enriching a cardiomyocyte from a cardiomyocyte-containing cell mixture without genetic alteration of a cardiomyocyte, producing a cardiomyocyte without genetic alteration of a cardiomyocyte, and evaluating the ratio of a cardiomyocyte in a cardiomyocyte-containing cell mixture.
    • 本发明涉及从源自全部心脏的细胞群体或源自干细胞的分化细胞群体中选择心肌细胞而不进行遗传改变的方法。 具体地说,本发明涉及一种基于细胞线粒体的相对含量和/或细胞的相对线粒体跨膜电位从含有心肌细胞的细胞混合物中选择心肌细胞而不对心肌细胞进行遗传改变的方法。 本发明还涉及从含有心肌细胞的细胞混合物中丰富心肌细胞的方法,而没有心肌细胞的遗传改变,产生心肌细胞而没有心肌细胞的遗传改变,以及评估心肌细胞含有心​​肌细胞的细胞混合物中的比例。