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41. 发明授权

US08257920B2 Method of detecting activation of notch signal transmission system 有权
标题翻译：检测陷波信号传输系统的激活方法
公开(公告)号：US08257920B2
公开(公告)日：2012-09-04
申请号：US11665264
申请日：2005-05-10
申请人： Hideyuki Okano , Akinori Tokunaga , Jun Kohyama , Keiko Nakao
发明人： Hideyuki Okano , Akinori Tokunaga , Jun Kohyama , Keiko Nakao
IPC分类号： C07H21/04 , C12N5/00 , C12N15/00 , C12N15/09 , C12Q1/68
CPC分类号： C12N15/86 , C12N2510/00 , C12N2740/15043
摘要： The purpose of the present invention is to provide detection methods of Notch signaling activation for detecting the activation of the Notch signaling in living cells simply and conveniently. The expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene which is controlled by the wild-type Hes-1 gene promoter has been introduced is compared with the expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene controlled by a mutated Hes-1 gene promoter which is not controlled by an activated Notch protein has been introduced, and a transgenic cell in which a signal by the expression of Venus introduced by the vector having the wild-type Hes-1 promoter is observed and in which a signal by the expression of Venus introduced by the vector having the mutated Hes-1 gene promoter which is not controlled by the activated Notch protein is not observed is identified.
摘要翻译：本发明的目的是提供用于在简单方便地检测活细胞中Notch信号传导的Notch信号传导的检测方法。将引入了由野生型Hes-1基因启动子控制的具有荧光蛋白维纳斯基因的载体的转基因细胞中的荧光蛋白金丝酵母的表达与转基因的荧光蛋白维纳斯的表达进行比较已经引入了具有由不受活化的Notch蛋白质控制的突变的Hes-1基因启动子控制的具有荧光蛋白Venus基因的载体的细胞，以及通过载体引入的金星的信号的转基因细胞观察到具有野生型Hes-1启动子的信号，其中没有观察到由未被活化的Notch蛋白质控制的具有突变的Hes-1基因启动子的载体引入的金星的表达信号。

42. 发明申请

US20110250684A1 METHOD FOR PRODUCING NEURAL STEM CELLS 有权
标题翻译：生产神经干细胞的方法
公开(公告)号：US20110250684A1
公开(公告)日：2011-10-13
申请号：US13127566
申请日：2009-11-04
申请人： Wado Akamatsu , Hideyuki Okano
发明人： Wado Akamatsu , Hideyuki Okano
IPC分类号： C12N5/0797
CPC分类号： C12N5/0623 , C12N2500/25 , C12N2500/46 , C12N2501/01 , C12N2501/115 , C12N2501/235 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2506/13 , C12N2506/1307
摘要： In order to provide a method for producing neural stem cells easily and quickly by inducing differentiation of somatic cells directly into neurospheres, dedifferentiation factors are introduced into somatic cells, which are then cultured in suspension in the presence of growth factors to produce the neurospheres, thereby allowing the neural stem cells to be produced quickly without establishing iPS cells.
摘要翻译：为了提供通过诱导体细胞直接分化成神经球来容易且快速地产生神经干细胞的方法，将去分化因子引入体细胞，然后在生长因子存在下将其分离培养以产生神经球，由此允许快速产生神经干细胞而不建立iPS细胞。

43. 发明授权

US07264793B2 Numb protein expression inhibitors by Musashi 有权
标题翻译： Musashi的Numb蛋白表达抑制剂
公开(公告)号：US07264793B2
公开(公告)日：2007-09-04
申请号：US10343311
申请日：2001-11-22
申请人： Takao Imai , Akinori Tokunaga , Tetsu Yoshida , Katsuhiko Mikoshiba , Masato Nakafuku , Hideyuki Okano
发明人： Takao Imai , Akinori Tokunaga , Tetsu Yoshida , Katsuhiko Mikoshiba , Masato Nakafuku , Hideyuki Okano
IPC分类号： A61K49/00 , A61K38/00 , C12P21/06 , C12N5/00 , C12N15/00 , C12N15/63
CPC分类号： C07K14/47 , A61K38/00
摘要： An Numb protein expression inhibitor comprising, as active ingredient, Musashi protein, a polypeptide having an amino acid sequence obtained by replacement, deletion, addition or insertion of at least one amino acid in an amino acid sequence of Musashi protein, or a gene encoding said polypeptide.By the present invention, a new function of Musashi protein has been elucidated. Described specifically, Musashi protein inhibits expression of Numb protein having a neuronal differentiation regulating function and potentiates the activity of the Notch signaling system, so that Musashi protein can be used as a therapeutic for various diseases of the central nervous system.
摘要翻译：一种Numb蛋白质表达抑制剂，其包含Musashi蛋白质作为活性成分的具有通过在Musashi蛋白质的氨基酸序列中替换，缺失，添加或插入至少一个氨基酸而获得的氨基酸序列的多肽，或编码所述多肽。通过本发明，已经阐明了Musashi蛋白质的新功能。具体地说，Musashi蛋白抑制具有神经元分化调节功能的Numb蛋白的表达，并增强Notch信号系统的活性，使得Musashi蛋白可以用作中枢神经系统各种疾病的治疗剂。

44. 发明申请

US20070098701A1 Methods for enhancing survival and/or proliferation of neural stem cells and neurite extension enhancers therefor pharmaceutical compostions containing neural stem cells assay methods and screening methods 有权
标题翻译：用于增强神经干细胞和神经突延伸增强剂的存活和/或增殖的方法，用于含有神经干细胞的药物组合物测定方法和筛选方法
公开(公告)号：US20070098701A1
公开(公告)日：2007-05-03
申请号：US10571277
申请日：2004-09-08
申请人： Hideyuki Okano , James Okano , Masanori Sakaguchi , Hidehiro Mizusawa , Satoru Ishibashi
发明人： Hideyuki Okano , James Okano , Masanori Sakaguchi , Hidehiro Mizusawa , Satoru Ishibashi
IPC分类号： A61K48/00 , C12N5/08
CPC分类号： C12N5/0623 , A61K35/30 , C12N2501/59
摘要： Problems to be Solved Methods for enhancing survival and/or proliferation of neural stem cells and pharmaceutical compositions containing neural stem cells prepared by such methods, together with methods for assaying factors enhancing survival and/or proliferation of neural stem cells and methods for screening for such factors. Means for Solving the Problem Either Galectin-1 is overexpressed in neural stem cells or neural stem cells are cultured in a liquid medium containing Galectin-1. Pharmaceutical compositions containing Galectin-1-overexpressing neural stem cells and pharmaceutical composition containing Galectin-1, prepared by the aforementioned methods, improve higher cerebral functions damaged by cerebral ischemia. Further, by seeding neural stem cells at clonal concentrations and determining whether the seeded neural stem cells are capable of proliferating in an assay medium to be assayed, whether the factor enhances survival and/or proliferation of neural stem cells is assayed and a factor enhancing survival and/or proliferation of neural stem cells are identified using this assay method.
摘要翻译：要解决的问题增强神经干细胞的存活和/或增殖的方法和通过这些方法制备的含有神经干细胞的药物组合物以及用于测定增强神经干细胞的存活和/或增殖的因子的方法以及用于筛选这些方法的方法因素解决问题的方法将Galectin-1在神经干细胞或神经干细胞中过表达，在含有Galectin-1的液体培养基中培养。含有Galectin-1-过表达神经干细胞的药物组合物和含有通过上述方法制备的Galectin-1的药物组合物改善了由脑缺血损伤的较高脑功能。此外，通过以克隆浓度接种神经干细胞并确定接种的神经干细胞是否能够在要测定的测定培养基中增殖，测定该因子是否增强神经干细胞的存活和/或增殖，并且提高生存的因子和/或使用该测定方法鉴定神经干细胞的增殖。

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