专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

41. 发明授权

US5283339A Immobilized metal aqueous two-phase extraction and precipitation 失效
标题翻译：固定金属水相两相萃取沉淀
公开(公告)号：US5283339A
公开(公告)日：1994-02-01
申请号：US925906
申请日：1992-08-05
申请人： Frances H. Arnold , Gerald E. Wuenschell
发明人： Frances H. Arnold , Gerald E. Wuenschell
IPC分类号： C07K1/22 , C08G65/332 , C08G65/333 , C07F1/08 , C07F3/00 , C07F15/00
CPC分类号： C07K1/22 , C08G65/332 , C08G65/33303
摘要： Metallated compounds can be used to extract or precipitate proteins from solution. The compounds can be polymers, such as polyalkylene glycols, which can be mono- or bimetallated or smaller bis-metal chelates, such as ethylenebis(oxyethylenenitrilo)tetraacetic acid. The monometallated polymers are preferred in the extraction process whereas the bimetallated compounds are preferred in the precipitation process. In addition, new PEG compounds which are capable of chelating a variety of metals are described. Both mono- and bi-metallated forms are set forth. The new metallated compounds are very effective in extracting or precipitating proteins from solution.
摘要翻译：金属化合物可用于从溶液中提取或沉淀蛋白质。化合物可以是聚合物，例如聚亚烷基二醇，其可以是单或双金属化或更小的双金属螯合物，例如亚乙基双（氧乙烯亚硝基）四乙酸。单金属化聚合物在提取过程中是优选的，而双金属化合物在沉淀过程中是优选的。此外，还描述了能够螯合各种金属的新型PEG化合物。阐述单和双金属化形式。新的金属化合物非常有效地从溶液中提取或沉淀蛋白质。

42. 发明授权

US09249401B2 Polypeptides having cellulase activity 有权
标题翻译：具有纤维素酶活性的多肽
公开(公告)号：US09249401B2
公开(公告)日：2016-02-02
申请号：US12755328
申请日：2010-04-06
申请人： Frances H. Arnold , Pete Heinzelman
发明人： Frances H. Arnold , Pete Heinzelman
IPC分类号： C12N9/42 , C12P19/14
CPC分类号： C12N9/2437 , C07K2319/00 , C12P19/14 , C12Y302/01004 , C12Y302/01091
摘要： The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
摘要翻译：本公开涉及CBH II嵌合融合多肽，编码多肽的核酸和用于产生多肽的宿主细胞。

43. 发明申请

US20150259657A1 STABLE FUNGAL CEL6 ENZYME VARIANTS 有权
公开(公告)号：US20150259657A1
公开(公告)日：2015-09-17
申请号：US13554736
申请日：2012-07-20
申请人： Frances H. Arnold , Indira Wu
发明人： Frances H. Arnold , Indira Wu
IPC分类号： C12N9/42 , C12P7/10 , C12P19/02
CPC分类号： C12N9/2437 , C12N15/00 , C12P7/00 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2203/00 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： The disclosure provides variant Cel6a enzymes having increased thermostability, methods of making and using such polypeptides.
摘要翻译：本公开提供了具有增加的热稳定性的变体Cel6a酶，制备和使用这些多肽的方法。

44. 发明申请

US20120171693A1 Methods for Generating Novel Stabilized Proteins 审中-公开
标题翻译：生成新型稳定蛋白的方法
公开(公告)号：US20120171693A1
公开(公告)日：2012-07-05
申请号：US11969894
申请日：2008-01-05
申请人： Frances H. Arnold , Yougen Li
发明人： Frances H. Arnold , Yougen Li
IPC分类号： C12Q1/68 , C12Q1/34 , C12Q1/40 , G06F19/20 , C12Q1/26 , C12Q1/28 , C12N9/00 , G06F19/14 , C12Q1/48 , C12Q1/37
CPC分类号： C12N9/0077 , G16B15/00 , G16B30/00
摘要： The disclosure provides methods for identifying and producing stabilized chimeric proteins.
摘要翻译：本公开提供了用于鉴定和产生稳定的嵌合蛋白的方法。

45. 发明授权

US08076114B2 Cytochrome P450 oxygenases 有权
标题翻译：细胞色素P450加氧酶
公开(公告)号：US08076114B2
公开(公告)日：2011-12-13
申请号：US12754216
申请日：2010-04-05
申请人： Edgardo T Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12P21/04 , C07H21/04 , C12Q1/26
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

46. 发明申请

US20100248327A1 CYTOCHROME P450 OXYGENASES 有权
标题翻译： CYTOCHROME P450氧化剂
公开(公告)号：US20100248327A1
公开(公告)日：2010-09-30
申请号：US12754216
申请日：2010-04-05
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C07H21/04
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

47. 发明授权

US07704715B2 Peroxide-driven cytochrome P450 oxygenase variants 有权
标题翻译：过氧化物驱动的细胞色素P450加氧酶变体
公开(公告)号：US07704715B2
公开(公告)日：2010-04-27
申请号：US12287681
申请日：2008-10-11
申请人： Patrick C. Cirino , Frances H. Arnold
发明人： Patrick C. Cirino , Frances H. Arnold
IPC分类号： C12N9/02 , C12Q1/26 , C12Q1/68 , C12N15/00 , C12N1/20 , C12P21/04 , C07H21/04
CPC分类号： C12N9/0071 , C12Y114/14001
摘要： The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.
摘要翻译：本发明涉及细胞色素P450加氧酶的新变体。与相应的野生型酶相比，这些变体具有改善使用过氧化物作为供氧体的能力。与细胞色素P450 BM-3 F87A突变体相比，这些变体也具有改善的热稳定性。优选的变体包括具有I58V，F87A，H100R，F107L，A135S，M145A / V，N239H，S274T，L324I，I366V，K434E，E442K和/或V446I氨基酸取代的细胞色素P450 BM-3血红素结构域突变体。

48. 发明申请

US20090298148A1 REGIO- AND ENANTIOSELECTIVE ALKANE HYDROXYLATION WITH MODIFIED CYTOCHROME P450 有权
标题翻译：具有改良的细胞色素P450的REGIO-和选择性碱性羟化
公开(公告)号：US20090298148A1
公开(公告)日：2009-12-03
申请号：US12424454
申请日：2009-04-15
申请人： Frances H. Arnold , Matthew W. Peters , Peter Meinhold
发明人： Frances H. Arnold , Matthew W. Peters , Peter Meinhold
IPC分类号： C12P7/02 , C12N9/02 , C12N15/53
CPC分类号： C12N9/0071 , C12N9/0077 , C12P7/04 , C12Y114/14001
摘要： Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio-and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.
摘要翻译：使用定向进化和定点诱变的组合将来自巨大芽孢杆菌的细胞色素P450 BM-3设计为使用大气二氧氧化物作为区域和对映选择性的羟基化线型烷烃。突变体9-10A-A328V主要在2-位羟基化辛烷值以形成S-2-辛醇（40％ee）。另一个突变体1-12G，主要在2-位羟基化烷烃大于己烷，但形成R-2-醇（40-55％ee）。这些生物催化剂对烷烃底物具有高活性，并支持数千种产品周转。这些区域和对映体选择性保留在与大肠杆菌的全细胞生物转化中，其中工程化的P450可以以高水平表达并且昂贵的辅因子是内源性提供的。

49. 发明申请

US20080293928A1 Cytochrom P450 oxygenases 有权
标题翻译：细胞色素P450加氧酶
公开(公告)号：US20080293928A1
公开(公告)日：2008-11-27
申请号：US11800970
申请日：2007-05-07
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C07H21/04
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

50. 发明申请

US20080268517A1 STABLE, FUNCTIONAL CHIMERIC CYTOCHROME P450 HOLOENZYMES 审中-公开
标题翻译：稳定的，功能性的CHIMERIC CYTOCHROME P450 HOLOENZYMES
公开(公告)号：US20080268517A1
公开(公告)日：2008-10-30
申请号：US12049318
申请日：2008-03-15
申请人： Frances H. Arnold , Yougen Li
发明人： Frances H. Arnold , Yougen Li
IPC分类号： C12N9/02 , C12N15/11 , C12N1/20 , C12N15/00
CPC分类号： C12N9/0077
摘要： The present disclosure relates to cytochrome p450 fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
摘要翻译：本公开涉及细胞色素p450融合多肽，编码多肽的核酸和用于产生多肽的宿主细胞。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式