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    • 42. 发明授权
    • Method for total intelligent parking/pollution and surveillance control system
    • 全智能停车/污染监控系统方法
    • US07071839B2
    • 2006-07-04
    • US10716023
    • 2003-11-18
    • Rajesh PatelSanjeev Nath
    • Rajesh PatelSanjeev Nath
    • G08G1/01
    • G07F17/246G06Q30/0284G07B15/02G08G1/14G08G1/205
    • The invention relates to systems to manage parking meters using mechanical, electrical and electromechanicals monitoring and transmitting devices. Such intelligent parking meters may be used for example to monitor pollution by idling vehicles, survey the area about the meters to identify events such as vandalism, accident or any other designated emergency situation, and to monitor volume and flow of traffic to help coordinate traffic light sequencing for facilitating optimum traffic movement. The meters may be coin, bill or card operated and are useful for monitoring individual parking spaces designed to monitor conditions ambient to the parking meter and issue summons to vehicles violating the preset requirements.
    • 本发明涉及使用机械,电气和机电监控和发射装置来管理停车计费器的系统。 这样的智能停车收费表可以用于监测怠速车辆的污染,调查仪表的区域,以识别事故,如破坏行为,事故或任何其他指定的紧急情况,并监测交通量和流量,以协助交通灯 排序,以促进最佳交通运动。 仪表可以是硬币,纸币或卡片操作,并且可用于监测专用于监视停车计时器周围环境的各个停车位,并向违反预设要求的车辆发出传票。
    • 50. 发明授权
    • Quantitative determination of nucleic acid amplification products
    • US06573054B2
    • 2003-06-03
    • US10043415
    • 2002-01-10
    • Rajesh PatelNurith Kurn
    • Rajesh PatelNurith Kurn
    • C12Q168
    • C12Q1/6851C12Q1/6818C12Q2565/519C12Q2527/101
    • The present invention relates to a method for detecting the amount of a target polynucleotide in a sample. A combination is provided in a medium. The combination comprises (i) a sample suspected of containing the target polynucleotide, the target polynucleotide being in single stranded form, (ii) a reference polynucleotide comprising a sequence that is common with a sequence of the target polynucleotide, and (iii) a predetermined amount of an oligonucleotide probe that has a sequence that hybridizes with the sequence that is common. The combination is subjected to conditions for amplifying the target polynucleotide and the reference polynucleotide. The conditions permit formation of substantially non-dissociative complexes of the target polynucleotide and the reference polynucleotide, respectively, with the oligonucleotide probe. Furthermore, the predetermined amount of the oligonucleotide probe is less than the expected amount of the amplified target polynucleotide. The ratio of the amount of the complex of the target polynucleotide with the oligonucleotide probe to the amount of the complex of the reference polynucleotide with the oligonucleotide probe is determined. Determination of the ratio is facilitated by employing second and third oligonucleotide probes. The second oligonucleotide probe has a sequence that hybridizes only with the second sequence of the target polynucleotide. The third oligonucleotide probe has a sequence that hybridizes only with a respective second sequence of the reference polynucleotide. The ratio is related to the known amount of the reference polynucleotide to determine the amount of the target polynucleotide in the sample. One or more reference polynucleotides may be employed with a corresponding third oligonucleotide probe for each reference probe. Kits for carrying out the above methods are also disclosed. The method is particularly applicable to the amplification and detection of RNA.