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41. 发明授权

US08901378B2 Corn event TC1507 and methods for detection thereof 有权
标题翻译：玉米事件TC1507及其检测方法
公开(公告)号：US08901378B2
公开(公告)日：2014-12-02
申请号：US12914271
申请日：2010-10-28
申请人： Eric Barbour , James Wayne Bing , Guy A. Cardineau , Robert F. Cressman, Jr. , Manju Gupta , Mary E. Hartnett Locke , David Hondred , Joseph W. Keaschall , Michael G. Koziel , Terry EuClaire Meyer , Daniel Moellenbeck , Kenneth Edwin Narva , Wilas Nirunsuksiri , Stephen W. Ritchie , Marjorie L. Rudert , Craig D. Sanders , Aihua Shao , Steven Jeffrey Stelman , David S. Stucker , Laura Ann Tagliani , William M. Van Zante
发明人： Eric Barbour , James Wayne Bing , Guy A. Cardineau , Robert F. Cressman, Jr. , Manju Gupta , Mary E. Hartnett Locke , David Hondred , Joseph W. Keaschall , Michael G. Koziel , Terry EuClaire Meyer , Daniel Moellenbeck , Kenneth Edwin Narva , Wilas Nirunsuksiri , Stephen W. Ritchie , Marjorie L. Rudert , Craig D. Sanders , Aihua Shao , Steven Jeffrey Stelman , David S. Stucker , Laura Ann Tagliani , William M. Van Zante
IPC分类号： A01H1/00 , C12N15/82 , C12Q1/68
CPC分类号： C12Q1/6895 , C12N15/8277 , C12N15/8286 , C12Q1/686 , C12Q2600/13 , C12Q2600/16 , Y02A40/162 , Y10S435/975
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize TC1507 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米TC1507事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

42. 发明授权

US08829279B2 Family of pesticidal proteins and methods for their use 有权
标题翻译：一系列杀虫蛋白及其使用方法
公开(公告)号：US08829279B2
公开(公告)日：2014-09-09
申请号：US13168454
申请日：2011-06-24
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： A01H5/00 , C12N15/82 , C07K14/325 , A01N37/18
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

43. 发明授权

US08563808B2 AXMI-003, a delta-endotoxin gene and methods for its use 有权
公开(公告)号：US08563808B2
公开(公告)日：2013-10-22
申请号：US11765491
申请日：2007-06-20
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N1/21 , C12N15/32
CPC分类号： C07H21/04 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

44. 发明授权

US08334431B2 AXMI-115, AXMI-113, AXMI-005, AXMI-163 and AXMI-184: insecticidal proteins and methods for their use 有权
标题翻译： AXMI-115，AXMI-113，AXMI-005，AXMI-163和AXMI-184：其杀虫蛋白及其使用方法
公开(公告)号：US08334431B2
公开(公告)日：2012-12-18
申请号：US12497221
申请日：2009-07-02
申请人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32 , C07K14/325 , A01N37/18
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予宿主细胞杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。组合物还包含转化的宿主细胞。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。

45. 发明授权

US08133858B2 AXMI-008, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-008，一种δ-内毒素基因及其使用方法
公开(公告)号：US08133858B2
公开(公告)日：2012-03-13
申请号：US12026427
申请日：2008-02-05
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： C07K14/325 , A01N37/18
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5和7所示氨基酸序列的核苷酸序列，以及SEQ ID NO：和6，以及其变体和片段。

46. 发明申请

US20110263488A1 FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE 有权
标题翻译：杀虫蛋白家族及其使用方法
公开(公告)号：US20110263488A1
公开(公告)日：2011-10-27
申请号：US13168454
申请日：2011-06-24
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas B. Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas B. Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： A01N63/02 , C12N15/63 , C12N1/21 , A01H5/10 , A01H5/00 , C07K14/325 , A01P5/00 , A01P7/04 , C07H21/04 , C12N5/10
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

47. 发明授权

US07811598B2 AXMI-028 and AXMI-029, a family of novel delta-endotoxin genes and methods for their use 有权
公开(公告)号：US07811598B2
公开(公告)日：2010-10-12
申请号：US12166253
申请日：2008-07-01
申请人： Nadine Carozzi , Michael G. Koziel , Tracy Hargiss , Nicholas B. Duck
发明人： Nadine Carozzi , Michael G. Koziel , Tracy Hargiss , Nicholas B. Duck
IPC分类号： A01N25/00 , A61K38/16 , C12P21/02
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

48. 发明授权

US07807881B2 Methods to confer herbicide resistance 失效
标题翻译：赋予除草剂抗性的方法
公开(公告)号：US07807881B2
公开(公告)日：2010-10-05
申请号：US11588811
申请日：2006-10-27
申请人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck , Michael G. Koziel
发明人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck , Michael G. Koziel
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8274 , C12N9/88 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.
摘要翻译：提供了赋予对植物细胞和细菌细胞的除草剂抗性的组合物和方法。所述方法包括用编码除草剂抗性基因的核苷酸序列转化细胞。特别地，通过与脱羧酶具有同源性的蛋白质的表达赋予除草剂抗性。组合物包括转化的植物，植物组织和种子，以及转化的细菌细胞。

49. 发明申请

US20100138961A1 AXMI-010, A DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
公开(公告)号：US20100138961A1
公开(公告)日：2010-06-03
申请号：US12701567
申请日：2010-02-07
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01H1/00 , C07H21/00 , C12N15/74 , C12N1/21 , C12N5/04 , A01H5/00 , C12P21/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.

50. 发明申请

US20100137216A1 AXMI-027, AXMI-036 AND AXMI-038, A FAMILY OF DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译： AXMI-027，AXMI-036和AXMI-038，一种三内酰胺素基因家族及其使用方法
公开(公告)号：US20100137216A1
公开(公告)日：2010-06-03
申请号：US12691115
申请日：2010-01-21
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck
IPC分类号： A61K38/16 , C07K14/195 , A01P15/00
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,11和13所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,10和12所示的核苷酸序列，作为以及其变体和片段。

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