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31. 发明申请

US20040091994A1 Alpha-amylase variant with altered properties 审中-公开
标题翻译：具有改变性质的α-淀粉酶变体
公开(公告)号：US20040091994A1
公开(公告)日：2004-05-13
申请号：US10399161
申请日：2003-04-11
发明人： Carsten Andersen
IPC分类号： C12N009/28 , C11D003/386 , C07H021/04 , C11D007/42 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C11D3/386 , C12N9/2417
摘要： The present invention relates to variants of parent alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, specific activity, and altered pI, in particular higher pI.
摘要翻译：本发明涉及亲本α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于所述亲本α-淀粉酶表现出至少一个以下特性的改变：底物特异性，底物结合，底物切割模式，热稳定性，pH /活性曲线，pH /稳定性曲线，对氧化的稳定性，比活性和改变的pI，特别是较高的pI。

32. 发明申请

US20040038841A1 Cellulase preparation containing nonionic surfactant and method of treating fiber 失效
标题翻译：含有非离子表面活性剂的纤维素酶制剂及纤维处理方法
公开(公告)号：US20040038841A1
公开(公告)日：2004-02-26
申请号：US10416328
申请日：2003-05-08
发明人： Jinichiro Koga , Akitaka Nakane , Yuko Baba , Toshiaki Kono
IPC分类号： C11D003/386
CPC分类号： D06P5/137 , C11D1/66 , C11D3/38645 , C12N9/2437 , C12Y302/01004 , D06L4/40 , D06M16/003 , D06P5/158 , D21C5/005 , D21C5/027 , Y02W30/648
摘要： The present invention relates to a cellulase preparation comprising nonionic surfactants together with endoglucanases derived from Zygomycetes; a detergent composition comprising the above cellulase preparation with detergent components; a method of treating fabric which comprises treating cellulose-containing fabric with the above cellulase preparation so as to improve the properties of the fabric; a method of deinking waster paper which comprises a step of treating waste paper with the above cellulase preparation together with a deinking agent; and a method of improving the freeness of paper pulp which comprises a step of treating paper pulp with the above cellulase preparation.
摘要翻译：本发明涉及包含非离子表面活性剂的纤维素酶制剂以及源自接合物的内切葡聚糖酶; 一种洗涤剂组合物，其包含具有洗涤剂成分的上述纤维素酶制剂; 一种处理织物的方法，其包括用上述纤维素酶制剂处理含纤维素的织物以改善织物的性能; 一种脱墨纸的方法，其包括用脱墨剂与上述纤维素酶制剂一起处理废纸的步骤; 以及提高纸浆的游离度的方法，其包括用上述纤维素酶制剂处理纸浆的步骤。

33. 发明申请

US20040029242A1 Process for immobilizing an enzyme 审中-公开
标题翻译：固定酶的方法
公开(公告)号：US20040029242A1
公开(公告)日：2004-02-12
申请号：US10637126
申请日：2003-08-08
申请人： The Procter & Gamble Company
发明人： Ming Tang , Yan Chen , Xiaoqing Wang , Xin Fu
IPC分类号： C11D003/386
CPC分类号： C11D3/38663
摘要： A process for immobilizing an enzyme includes the steps of selecting a supporting substrate, activating the supporting substrate with an activating molecule to form an activated supporting substrate; adding an enzyme and the activated supporting substrate to an organic solvent to form a mixture and obtaining an immobilized enzyme from the mixture. The organic solvent contains from about 0.01% to about 30% by weight of the organic solvent of water.
摘要翻译：固定酶的方法包括选择支撑基底，用活化分子活化支撑基底以形成活化的支撑基底的步骤; 将酶和活化的支持底物加入到有机溶剂中以形成混合物并从混合物中获得固定化酶。有机溶剂含有约0.01％至约30％重量的水的有机溶剂。

34. 发明申请

US20030211958A1 Alpha-amylase mutants 有权
标题翻译： α-淀粉酶突变体
公开(公告)号：US20030211958A1
公开(公告)日：2003-11-13
申请号：US10327837
申请日：2002-12-23
申请人： Novozymes A/S
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
IPC分类号： C11D003/386 , C07H021/04 , C12N009/28 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2null concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2null concentrations (relative to the parent).
摘要翻译：本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中具有增加的热稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供了一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）表现出增加的热稳定性。

35. 发明申请

US20030175940A1 Cell-wall degrading enzyme variants 失效
标题翻译：细胞壁降解酶变体
公开(公告)号：US20030175940A1
公开(公告)日：2003-09-18
申请号：US10403192
申请日：2003-03-31
申请人： Novozymes A/S
发明人： Sanne O. Schroder Glad , Carsten Andersen , Martin Schulein , Hanne Dela , Torben Peter
IPC分类号： C12N009/88 , G01N033/53 , G06F019/00 , G01N033/48 , G01N033/50 , C07H021/04 , C12N001/21 , C11D003/386 , C12N015/74
CPC分类号： C11D3/38636 , C12N9/88
摘要： The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.
摘要翻译：本发明涉及具有β-螺旋结构的细胞壁降解酶的变体，该变体具有至少一个取代基，其位置通过鉴定潜在属于堆叠的所有残基而确定; 将堆栈表征为内部或外部; 基于母体或野生型酶堆残基的主导特征和/或其相对于β-螺旋（内部或外部）的取向，将所述叠层表征为极性，疏水性或芳族/杂芳族化合物; 通过在属于这些基团之一的氨基酸的一个或所有位置内允许突变将堆叠的所有堆叠位置优化为疏水性脂族氨基酸，疏水性芳族或极性氨基酸; 通过DSC测量变体的热稳定性或应用相关测定，例如Pad-Steam应用测试; 并选择稳定的变体。当与包含SEQ ID NO：1的氨基酸序列的果胶酸裂解酶比对时，具有保守氨基酸残基D111，D141或E141，D145，K165，R194和R199的野生型亲本果胶酸裂合酶（EC 4.2.2.2） 2是优选的。

36. 发明申请

US20030113734A1 EGVII endoglucanase and nucleic acids encoding the same 有权
标题翻译： EGVII内切葡聚糖酶和编码相同的核酸
公开(公告)号：US20030113734A1
公开(公告)日：2003-06-19
申请号：US10028244
申请日：2001-12-18
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
IPC分类号： C12Q001/68 , C07H021/04 , C12P007/06 , C12N009/42 , C12N001/16 , C12P021/02 , C11D003/386 , C12N015/74
CPC分类号： C12N9/2437 , C11D3/38 , C11D3/38636 , C11D3/38645 , C12N9/2445 , C12N15/1137 , C12N2310/11 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2201/00 , C12Y302/01004 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
摘要翻译：本发明提供了一种命名为egl7的新型内切葡聚糖酶核酸序列和相应的EGVII氨基酸序列。本发明还提供了包含编码EGVII的核酸序列，重组EGVII蛋白的表达载体和宿主细胞及其制备方法。

37. 发明申请

US20030096723A1 Protease obtained from Vibrio metschnikovii variants and detergent compositions comprising the above protease 有权
标题翻译：从弧菌属细菌变种获得的蛋白酶和包含上述蛋白酶的洗涤剂组合物
公开(公告)号：US20030096723A1
公开(公告)日：2003-05-22
申请号：US10228040
申请日：2002-08-27
发明人： Ghee-Hong Jin , Sung-Hoo Jhon , Hyun-Hwan Lee , Hyune-Mo Rho
IPC分类号： C11D003/386
CPC分类号： C12N9/52
摘要： The present invention relates to a Vibrio metscnikovii variants, more particularly to a biochemical characteristics of protease which is obtained from Vibrio metscnikovii variant strains such as Vibrio metschnikovii KS1, Vibrio metschnikovii KS1 transformed with pDSBCm, Vibrio metschnikovii KS1 transformed with pSBCm and Vibrio metschnikovii RH530 N-4-8 strain, the mother strain of Vibrio metschnikovii and to a detergent composition including the protease.
摘要翻译：本发明涉及弧菌弧菌变体，更具体地涉及从弧菌弧菌变种株如Vibrio metschnikovii KS1，用pDSBCm转化的弧菌弧菌KS1，用pSBCm转化的弧菌弧菌KS1和弧菌弧菌RH530N获得的蛋白酶的生化特性 -4-8菌株，弧菌弧菌的母体菌株和包含蛋白酶的洗涤剂组合物。

38. 发明申请

US20030087785A1 Modified polypeptides 审中-公开
标题翻译：修饰的多肽
公开(公告)号：US20030087785A1
公开(公告)日：2003-05-08
申请号：US10209812
申请日：2002-08-01
申请人： Novozymes A/S
发明人： Arne Agerlin Olsen , Claus von der Osten , Kim Vilbour Andersen , Steffen Ernst , Erwin Ludo Roggen
IPC分类号： C11D003/386 , C07H021/04 , C12N009/24 , C12N009/50 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C12N9/242 , A61K47/54 , A61K47/60 , A61K47/646 , C11D3/3719 , C11D3/38 , C12N9/54
摘要： The present invention relates to polypeptides with reduced immune response including reduced allergenicity having one or more amino acid residues being substituted with other amino acid residues and/or having coupled one or more polymeric molecules in the vicinity of the polypeptides metal binding site, a method for preparing modified polypeptides of the invention, the use of the polypeptide for reducing the immunogenicity and allergenicity and compositions comprising the polypeptide.
摘要翻译：本发明涉及具有降低的免疫应答的多肽，包括具有一个或多个氨基酸残基被其它氨基酸残基取代的降低的变应原性和/或在多肽金属结合位点附近具有偶联的一个或多个聚合物分子的方法，制备本发明的修饰多肽，多肽用于降低免疫原性和变应原性的用途以及包含该多肽的组合物。

39. 发明申请

US20030064908A1 Amylase variants 失效
标题翻译：淀粉酶变体
公开(公告)号：US20030064908A1
公开(公告)日：2003-04-03
申请号：US10025648
申请日：2001-12-19
申请人： Novozymes A/S
发明人： Henrik Bisgard-Frantzen , Allan Svendsen , Torben Vedel Borchert
IPC分类号： C11D003/00 , C11D003/386 , C12N009/00
CPC分类号： C11D3/38618 , C11D3/38609 , C11D3/38681 , C12N9/2417 , C12Y302/01001 , D06L1/14
摘要： The present invention relates to variants of a parent null-amylase, which parent null-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an null-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an a-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent null-amylase has been deleted; and/or (b) at least one amino acid residue of the parent null-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent null-amylase; the variant having null-amylase activity and exhibiting at least one of the following properties relative to the parent null-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2null dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.
摘要翻译：本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下特性中的至少一个：增加的热稳定性; 提高氧化稳定性; 并减少Ca2 +依赖; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。

40. 发明申请

US20020173441A1 Enzyme-containing granules and process for the production thereof 失效
公开(公告)号：US20020173441A1
公开(公告)日：2002-11-21
申请号：US10101123
申请日：2002-03-19
申请人： Novozymes A/S
发明人： Joao F. De Lima , Eder Manzini Bordin , Erik Kjaer Markussen , Kirsten Boegh Levring , Michael Bonde , Erik Marcussen , Grethe Saugmann
IPC分类号： C11D017/00 , C11D003/386
CPC分类号： A21D8/042 , A23K20/189 , C11D3/222 , C11D3/38672 , C11D17/0039 , C12N9/16 , C12N9/98 , C12N11/10 , C12N11/14 , D06L4/40 , D06M16/003 , D06P1/0004 , D06P5/02 , D06P5/158
摘要： The present invention relates to enzyme-containing granules comprising (a) an enzyme and (b) a core which intrinsically is capable of absorbing at least 5% w/w (based on the weight of the core) of water and to processes for the production of such granules comprises (a) contacting absorbent cores, capable of absorbing at least 5% w/w (based on the weight of the core) of water, with a liquid medium, such as an aqueous medium, containing an enzyme in dissolved and/or dispersed form, the amount of the liquid medium employed being such that substantially no attendant agglomeration of the resulting product occurs; and (b) at least partially removing volatile components of the liquid medium from the resulting product.

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