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31. 发明授权

US08771931B2 Perfusion culture method and perfusion culture device for organ or tissue 有权
标题翻译：灌注培养法和器官或组织灌注培养装置
公开(公告)号：US08771931B2
公开(公告)日：2014-07-08
申请号：US13574855
申请日：2011-01-25
申请人： Takashi Tsuji , Kazuhisa Nakao
发明人： Takashi Tsuji , Kazuhisa Nakao
IPC分类号： A01N1/00
CPC分类号： A01N1/0247 , C12M21/08 , C12M29/10
摘要： Disclosed are a perfusion culture method and a perfusion culture device whereby a perfusate can be delivered to all parts of an organ or tissue so that the organ or tissue can be preserved while sufficiently maintaining the function thereof. Specifically disclosed is a method for perfusion culture of an organ or tissue removed from a mammal, said organ or tissue having been removed together with a second organ or tissue connected to the aforesaid organ or tissue in vivo, which comprises a step for fixing said second organ or tissue so as to hang the aforesaid organ or tissue and a step for perfusing blood vessels in the aforesaid organ or tissue with a perfusate.
摘要翻译：公开了一种灌注培养方法和灌注培养装置，其中灌注液可以递送到器官或组织的所有部分，使得可以在充分保持其功能的同时保存器官或组织。具体公开了一种用于从哺乳动物，所述器官或组织移除的器官或组织的灌注培养的方法，所述器官或组织与连接到所述前述器官或组织的体内的第二器官或组织一起被去除，其包括用于固定所述第二器官或组织，以悬挂上述器官或组织，以及用灌注液灌注前述器官或组织中的血管的步骤。

32. 发明授权

US08765467B2 Multicellular compositions of pluripotent human embryonic stem cells and cancer cells 失效
标题翻译：多能人胚胎干细胞和癌细胞的多细胞组合物
公开(公告)号：US08765467B2
公开(公告)日：2014-07-01
申请号：US10555537
申请日：2004-05-05
申请人： Karl L. Skorecki , Maty Tzukerman
发明人： Karl L. Skorecki , Maty Tzukerman
IPC分类号： A01N1/00 , C12N5/00 , C12N5/07
CPC分类号： G01N33/5011 , A61K49/0008 , C12N5/0606 , C12N5/0693 , C12N2501/115 , C12N2502/02 , G01N33/57492 , G01N2500/10
摘要： Methods are provided for producing novel multicellular compositions comprising cancer cells together with pluripotent human stem cells, which are capable of proliferating and differentiating into various normal cell lines and tissue structures. These novel multicellular compositions are useful for investigating the properties of cancer cells in a normal human tissue microenvironment, and for studying interventions that will modulate these properties including devising, testing and screening therapeutic drugs.
摘要翻译：提供了用于生产新型多细胞组合物的方法，其包含癌细胞以及能够增殖和分化成各种正常细胞系和组织结构的多能人干细胞。这些新颖的多细胞组合物可用于研究正常人组织微环境中癌细胞的性质，并用于研究将调节这些性质的干预，包括设计，测试和筛选治疗药物。

33. 发明授权

US08741555B2 Apparatus and method for perfusion and determining the viability of an organ 有权
标题翻译：用于灌注和确定器官活力的装置和方法
公开(公告)号：US08741555B2
公开(公告)日：2014-06-03
申请号：US10845154
申请日：2004-05-14
申请人： John Brassil , Simona Baicu
发明人： John Brassil , Simona Baicu
IPC分类号： A01N1/00 , A01N1/02
CPC分类号： A61B5/14525 , A01N1/02 , A01N1/0247 , A61B5/413
摘要： An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. During the period in which the organ is preserved and/or maintained, the organ may be additionally be perfused to obtain data regarding the fluid and/or organ. The data may then be used to ultimately provide information regarding the perfusion efficacy and allow for altering of the perfusion parameters.
摘要翻译：器官灌注装置和方法监测，维持和/或恢复器官的活力并保存器官用于储存和/或运输。其他装置包括器官输送器，器官盒和器官诊断装置。该方法包括在低温和/或正常体温温度下灌注器官，优选在用于器官运输和/或储存的低温器官冲洗之后。该方法可以在先前或随后的器官的静态或灌注低温暴露下进行。在保存和/或保持器官的期间，可以另外灌注器官以获得关于流体和/或器官的数据。然后可以使用数据来最终提供关于灌注功效的信息并允许改变灌注参数。

34. 发明授权

US08722403B2 Specimen preservation chamber and method 有权
标题翻译：样品保存室和方法
公开(公告)号：US08722403B2
公开(公告)日：2014-05-13
申请号：US13353914
申请日：2012-01-19
申请人： Sanjay S. Kumar
发明人： Sanjay S. Kumar
IPC分类号： A01N1/00
CPC分类号： A01N1/0252 , A01N1/0263 , A61B10/0096
摘要： The present invention comprises a specimen preserver. A bubble reservoir is used to hold a preserving liquid such as artificial cerebrospinal liquid (aCSF). Oxygen is introduced into the aCSF by a gas diffuser which is attached to the bubble reservoir. The tissue samples are contained in one or more chambers in a holding platform. The platform is suspended well above the bottom of the bubble reservoir. The lower portion of each of the chambers is open toward the bottom of the bubble reservoir, but is covered by a fine mesh. The gas introduced into the aCSF circulates within the bubble reservoir. However, the tissue samples are not directly exposed to the gas bubbles because the fine mesh excludes the bubbles from the chambers in which the tissue samples are housed.
摘要翻译：本发明包括样品保存器。使用气泡储存器容纳诸如人造脑脊液（aCSF）的保存液体。氧气通过连接到气泡储存器的气体扩散器引入到aCSF中。组织样品包含在保持平台中的一个或多个室中。平台悬浮在气泡储存器底部的上方。每个室的下部朝向气泡储存器的底部开口，但被细网覆盖。引入aCSF的气体在气泡储存器内循环。然而，组织样品不直接暴露于气泡，因为细网不排除来自容纳组织样本的腔室中的气泡。

35. 发明授权

US08685709B2 Fluidics based pulsatile perfusion preservation device and method 有权
标题翻译：基于流体的脉动灌注保存装置及方法
公开(公告)号：US08685709B2
公开(公告)日：2014-04-01
申请号：US12890348
申请日：2010-09-24
申请人： Leonid Bunegin , Edward F. Gelineau
发明人： Leonid Bunegin , Edward F. Gelineau
IPC分类号： A01N1/00 , A01N1/02
CPC分类号： A01N1/0247
摘要： In one embodiment, a preservation apparatus is described that includes a storage compartment. The storage compartment is configured to hold an organ or tissue and a preservation fluid. A cover assembly is configured to engage the storage compartment. The cover assembly includes a support element, wherein the support element together with the storage compartment define a storage chamber. The cover assembly also includes a lid and a gas permeable membrane disposed between the lid and the support element. The gas permeable membrane and the support element together define a perfusion chamber configured to hold preservation fluid and an organ or tissue during use.
摘要翻译：在一个实施例中，描述了包括存储隔间的保存装置。储存室构造成保持器官或组织和保存液体。盖组件构造成接合储藏室。所述盖组件包括支撑元件，其中所述支撑元件与所述储存室一起限定存储室。盖组件还包括盖和设置在盖和支撑元件之间的透气膜。气体可渗透膜和支撑元件一起限定了在使用期间构造成保持保存流体和器官或组织的灌注室。

36. 发明授权

US08685635B2 Dermal micro-organs, methods and apparatuses for producing and using the same 失效
标题翻译：真皮微器官，用于制造和使用它们的方法和装置
公开(公告)号：US08685635B2
公开(公告)日：2014-04-01
申请号：US13656832
申请日：2012-10-22
申请人： Medgenics Medical Israel Ltd.
发明人： Stephen F. Bellomo , Itzhak Lippin , Guillermo Alberto Piva , Lior Rosenberg , Mordechay Bukhman , Baruch S. Stern , David Shalhevet , Menachem D. Shavitt , Andrew L. Pearlman , Noam Shani , Einat Almon
IPC分类号： A01N1/00 , C12N5/00 , A61F2/10
CPC分类号： A61B17/3468 , A61B10/0275 , A61B17/32053 , A61B17/322 , A61B2017/0023 , A61B2017/00969 , A61B2017/2808 , A61B2017/320052 , A61B2017/320064 , A61B2017/3225 , A61B2217/005 , A61F2/105 , A61K35/36 , A61K38/1816 , A61K38/193 , A61K38/2066 , A61K38/215 , A61K38/27 , A61K38/28 , A61M5/425 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2510/00 , Y10T83/929
摘要： Embodiments of the present invention provide Dermal Micro-organs (DMOs), methods and apparatuses for producing the same. Some embodiments of the invention provide a DMO including a plurality of dermal components, which substantially retain the micro-architecture and three dimensional structure of the dermal tissue from which they are derived, having dimensions selected so as to allow passive diffusion of adequate nutrients and gases to cells of the DMO and diffusion of cellular waste out of the cells so as to minimize cellular toxicity and concomitant death due to insufficient nutrition and accumulation of waste in the DMO. Some embodiments of the invention provide methods and apparatuses for harvesting the DMO. An apparatus for harvesting the DMO may include, according to some exemplary embodiments, a support configuration to support a skin-related tissue structure from which the DMO is to be harvested, and a cutting tool able to separate the DMO from the skin-related tissue structure. Other embodiments are described and claimed.
摘要翻译：本发明的实施方案提供了皮肤微器官（DMO），其制备方法和装置。本发明的一些实施方案提供了包含多个皮肤成分的DMO，其基本上保留了衍生自它们的真皮组织的微结构和三维结构，其尺寸被选择为允许适当营养物和气体的被动扩散到DMO的细胞并将细胞废物扩散出细胞，以便最小化细胞毒性和由于DMO中的营养不足和废物积聚引起的伴随死亡。本发明的一些实施例提供了用于收获DMO的方法和装置。根据一些示例性实施例，用于收获DMO的装置可以包括用于支撑要从其中收获DMO的与皮肤相关的组织结构的支撑构型，以及能够将DMO与皮肤相关组织分离的切割工具结构体。描述和要求保护其他实施例。

37. 发明授权

US08673547B2 Method for isolation of afterbirth derived cells 有权
标题翻译：分离产后细胞的方法
公开(公告)号：US08673547B2
公开(公告)日：2014-03-18
申请号：US12530236
申请日：2008-03-07
申请人： Frederick A. Gage
发明人： Frederick A. Gage
IPC分类号： A01N1/00 , A01N1/02 , C12M1/00
CPC分类号： C12N5/0605 , C12N2509/00
摘要： A method for extracting cells from afterbirth tissue, including placing the afterbirth into a perfusion circuit prior to exsanguination, extracting the cells from the afterbirth with digestive enzymes and mechanically recovering cells from the digested afterbirth tissue, and isolating the cells from the perfusate and digestion mix. Also disclosed is a cell line derived from afterbirth using the two-step pulsatile perfusion extraction method.
摘要翻译：一种用于从分娩后组织中提取细胞的方法，包括在分娩前将分娩置于灌注回路中，用消化酶从分娩后提取细胞并从消化后的组织中机械回收细胞，并从灌注液和消化混合物中分离细胞。还公开了使用两步骤脉冲灌注提取方法从分娩得到的细胞系。

38. 发明授权

US08658348B2 Spatially-defined modification of fresh tissue using covalent chemistry 有权
标题翻译：使用共价化学的空间定义的新鲜组织修饰
公开(公告)号：US08658348B2
公开(公告)日：2014-02-25
申请号：US13206403
申请日：2011-08-09
申请人： Robert R. Kane , Aruna Perera
发明人： Robert R. Kane , Aruna Perera
IPC分类号： A01N1/00 , A61K47/00
CPC分类号： A61L27/3687 , A61K35/12 , C12N5/0006
摘要： Methods for modification of tissue using covalent chemistry. Tissue can be modified through direct alkylation, reduction followed by alkylation, or oxidation followed by condensation to covalently attach small organic molecules or appropriately modified proteins. The modification can be spatially limited to desired regions of the tissue surface.
摘要翻译：使用共价化学修饰组织的方法。组织可以通过直接烷基化，还原后进行烷基化或氧化，然后进行缩合以共价连接小有机分子或适当修饰的蛋白质进行修饰。该修饰可以在空间上限于组织表面的期望区域。

39. 发明授权

US08481276B2 Ex vivo method for determining potential GLP-2 receptor modulators 失效
公开(公告)号：US08481276B2
公开(公告)日：2013-07-09
申请号：US13565618
申请日：2012-08-02
申请人： Lidia L. Demchyshyn , Hong Wang
发明人： Lidia L. Demchyshyn , Hong Wang
IPC分类号： G01N33/567 , A01N1/00 , A01N1/02
CPC分类号： G01N33/5082 , G01N33/5088 , G01N33/74 , G01N2333/605 , G01N2333/726 , G01N2500/04 , G01N2500/10
摘要： Disclosed herein is a method for measuring the contractility of intestinal tissue upon treatment with GLP-2 or a GLP-2 ligand. Also disclosed is an assay which directly measures the activity of GLP-2 or GLP-2 ligands ex vivo and permits the screening of putative GLP-2 ligands in native tissue.

40. 发明授权

US08460859B2 Tissue preservation and fixation method 有权
标题翻译：组织保存和固定方法
公开(公告)号：US08460859B2
公开(公告)日：2013-06-11
申请号：US12447773
申请日：2007-10-26
申请人： Virginia A. Espina , Lance A. Liotta , David Geho
发明人： Virginia A. Espina , Lance A. Liotta , David Geho
IPC分类号： A01N1/00
CPC分类号： G01N1/30 , A01N1/00
摘要： This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that is effective to fix the phosphoproteins, and that has a sufficient water content to be soluble for a stabilizer and/or a permeability enhancing agent); (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; and (3) a permeability enhancing agent (e.g. PEG). Methods are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. the following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample.
摘要翻译：本发明涉及例如在室温下与包含磷蛋白的样品接触时可以固定和稳定细胞磷酸蛋白的组合物，保留细胞形态，并允许样品冷冻以产生适于分子的低温恒温器冷冻切片分析。组合物包含（1）固定磷酸蛋白有效的固定剂，并且其具有足够的水含量可溶于稳定剂和/或渗透性增强剂）; （2）稳定剂，其包含（a）激酶抑制剂和（b）磷酸酶抑制剂和任选的（c）蛋白酶（例如蛋白酶）抑制剂; 和（3）渗透性增强剂（例如PEG）。描述了使用这种组合物来保存磷蛋白的方法。还描述了用于监测蛋白质降解的内源替代标记，包括翻译后修饰（例如磷酸化）的丧失，例如，以下从受试者中除去细胞或组织; 和外源性分子前哨蛋白（例如连接到磁性纳米颗粒的磷酸蛋白），其允许评价细胞或组织群体样品的加工历史。

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