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    • 33. 发明申请
    • METHOD FOR DETECTING ANTIGEN, AND APPARATUS FOR DETECTING ANTIGEN USING THE SAME, AND MICROFLUIDIC CHIP USING THE SAME
    • 用于检测抗原的方法和用于检测抗原的装置以及使用其的微流感芯片
    • US20110086436A1
    • 2011-04-14
    • US12900316
    • 2010-10-07
    • Nam Soo MYUNG
    • Nam Soo MYUNG
    • G01N33/566C40B60/12G01N30/00
    • G01N33/54346B01F13/0059B01F13/0818B01L3/50273B01L2400/043G01N33/54333
    • A method for detecting an antigen, an apparatus for detecting an antigen using the same, and a microfluidic chip using the same are disclosed. The method for detecting an antigen, includes: binding a first antibody and nano-beads to generate antibody nano-beads; binding the generated antibody nano-beads and an antigen to generate antigen-antibody nano-beads; forming at least one of an electric field and a magnetic field on the generated antigen-antibody nano-beads to bind the generated antigen-antibody nano-beads and a second antibody; and detecting the antigen-antibody nano-beads bound to the second antibody. Thus, when nano-beads affected by an electromagnetic field exist within the electromagnetic field that temporally and spatially changes, the nano-beads move according to non-uniformity of the electromagnetic field. In particular, an active mixing can be performed by using the electromagnetic field which is spatially non-uniform and changes temporally, a reaction time can be reduced. In addition, a flow can be controlled to make nano-beads move to a capture antibody, thus detecting an antigen with a small amount of a test sample within a short time.
    • 公开了一种用于检测抗原的方法,使用其的抗原检测装置和使用该抗原的微流控芯片。 检测抗原的方法包括:结合第一抗体和纳米珠以产生抗体纳米珠; 结合生成的抗体纳米珠和抗原以产生抗原 - 抗体纳米珠; 在产生的抗原 - 抗体纳米珠上形成电场和磁场中的至少一个以结合所产生的抗原 - 抗体纳米珠和第二抗体; 并检测与第二抗体结合的抗原 - 抗体纳米珠。 因此,当由电磁场影响的纳米珠存在于时间和空间变化的电磁场内时,纳米珠根据电磁场的不均匀性而移动。 特别地,可以通过使用在空间上不均匀并且在时间上变化的电磁场来执行主动混合,从而可以减少反应时间。 此外,可以控制流动以使纳米珠移动到捕获抗体,从而在短时间内用少量的测试样品检测抗原。
    • 37. 发明申请
    • MICROFLUIDIC DEVICE
    • 微流体装置
    • US20100216126A1
    • 2010-08-26
    • US12442993
    • 2008-01-12
    • Wamadeva BalachandranSayyed Mohamad AzimiJeremy AhernMassoud ZolgharniMohammad Reza BahmanyarPredrag Slijepcevic
    • Wamadeva BalachandranSayyed Mohamad AzimiJeremy AhernMassoud ZolgharniMohammad Reza BahmanyarPredrag Slijepcevic
    • C12Q1/68B81B7/02G01N33/50G01N1/28G01N1/34C12M1/00
    • B01L3/50273B01F13/0059B01F13/0818B01L3/502746B01L3/502761B01L2200/0647B01L2300/0645B01L2300/0809B01L2300/087B01L2400/043Y10T436/25
    • A microfluidic device comprising; i) an inlet; ii) a first layer comprising at least first and second current carrying structures, wherein the at least first and second current carrying structures each comprise a plurality of teeth, and wherein the teeth of the first and second current carrying structures are optionally offset such that the teeth of the first current carrying structure are positioned between the teeth of the second current carrying structure; iii) a second layer comprising a first microfluidic chamber in fluid communication with the inlet positioned above the at least first and second current carrying structures of the first layer; and iv) a third layer comprising at least third and fourth current carrying structures wherein the at least third and fourth current carrying structures each comprise a plurality of teeth, and wherein the teeth of the third and fourth current carrying structures are optionally offset such that the teeth of the third current carrying structure are positioned between the teeth of the fourth current carrying structure; and wherein the at least third and fourth current carrying structures are positioned in the third layer so as to be above the first microfluidic chamber and such that the teeth of the third current carrying structure are positioned substantially vertically above or offset from the teeth of the first current carrying structure and the teeth of the fourth current carrying structure are positioned substantially vertically above or offset from the teeth of the second current carrying structure; wherein the teeth have a stem having substantially elliptical tip.
    • 一种微流体装置,包括: i)入口; ii)包括至少第一和第二载流结构的第一层,其中所述至少第一和第二载流结构各自包括多个齿,并且其中所述第一和第二载流结构的齿可选地偏移,使得 第一载流结构的齿位于第二载流结构的齿之间; iii)第二层,包括与位于第一层的至少第一和第二载流结构之上的入口流体连通的第一微流体室; 以及iv)包括至少第三和第四载流结构的第三层,其中所述至少第三和第四载流结构各自包括多个齿,并且其中所述第三和第四载流结构的齿可选地偏移,使得 第三载流结构的齿位于第四载流结构的齿之间; 并且其中所述至少第三和第四载流结构定位在所述第三层中以便在所述第一微流体室的上方,并且使得所述第三载流结构的齿基本垂直地定位在所述第一 载流结构和第四载流结构的齿基本垂直地定位在第二载流结构的齿之上或偏移上; 其中所述齿具有具有基本上椭圆形尖端的杆。