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31. 发明授权

US6043039A Method of and composite for in situ fluorescent hybridization 失效
标题翻译：原位荧光杂交法及复合方法
公开(公告)号：US6043039A
公开(公告)日：2000-03-28
申请号：US122661
申请日：1998-07-27
申请人： Irit Bar-Am , Cathy Janish , Yuval Garini , Dario Cabib , Robert A. Buckwald
发明人： Irit Bar-Am , Cathy Janish , Yuval Garini , Dario Cabib , Robert A. Buckwald
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N33/566
CPC分类号： C12Q1/6841
摘要： A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.
摘要翻译：一种荧光原位杂交方法，包括以下步骤：（a）获得物种的染色体扩散; （b）制备含有多个染色体涂料的杂化复合物，所述多个染色体涂料中的每一个用不同的荧光团或荧光团组合进行标记，使得所述杂交复合物中高度重复序列的平均比活性基本上等于杂交复合物中独特序列的平均比活性; （c）使杂交复合物变性并对杂交复合物进行条件以允许杂交复合物中的至少一部分高度重复序列进行再冷凝，同时杂交复合物中至少一部分独特序列保持单链; （d）在杂交条件下使杂交复合物与染色体扩散接触; （e）洗去杂交组合物的过量; 和（d）分析和呈现现在杂交的染色体扩增的图像。

32. 发明授权

US5912165A Method for chromosome classification by decorrelaiton statistical analysis and hardware therefore 失效
标题翻译：因此，通过去相关统计分析和硬件进行染色体分类的方法
公开(公告)号：US5912165A
公开(公告)日：1999-06-15
申请号：US942549
申请日：1997-10-01
申请人： Dario Cabib , Robert A. Buckwald , Nissim Ben-Yosef
发明人： Dario Cabib , Robert A. Buckwald , Nissim Ben-Yosef
IPC分类号： C12Q1/68 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/453 , G01N21/64 , G01N33/50 , G01N33/569 , G06K9/00 , G06K9/76 , C12M1/34 , F21V9/08 , G02B21/16
CPC分类号： G01N21/6458 , C12Q1/6841 , C12Q1/6883 , G01J3/12 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01N21/6428 , G01N33/5005 , G01N33/56966 , G06K9/00127 , G06K9/76 , C12Q2600/156 , G01J2003/2866 , G01J3/02 , G01N2021/6417
摘要： A method and hardware for chromosome classification by decorrelation statistical analysis to provide color (spectral) karyotypes and to detect chromosomal aberrations. The method and hardware employ a set of N decorrelation matched filters for chromosome classification. The N decorrelation matched filters are dedicated for extracting decorrelated spectral data from chromosome samples painted according to a specific experimental protocol. The N decorrelation matched filters being described by: ##EQU1## where V.sub.ik min equals minimum V.sub.ik over all i, and V.sub.ik max equals maximum V.sub.ik over all i, and wherein N is an integer greater than two.
摘要翻译：通过去相关统计分析进行染色体分类的方法和硬件，以提供颜色（光谱）核型和检测染色体畸变。该方法和硬件采用一组N个去相关匹配滤波器进行染色体分类。 N去相关匹配滤波器专用于从根据特定实验方案绘制的染色体样品中提取去相关光谱数据。 N个去相关匹配滤波器由以下描述：其中Vik min等于所有i上的最小Vik，Vik max等于所有i上的最大Vik，并且其中N是大于2的整数。

33. 发明授权

US5784162A Spectral bio-imaging methods for biological research, medical diagnostics and therapy 失效
标题翻译：用于生物研究，医学诊断和治疗的光谱生物成像方法
公开(公告)号：US5784162A
公开(公告)日：1998-07-21
申请号：US571047
申请日：1995-12-12
申请人： Dario Cabib , Robert A. Buckwald , Zvi Malik , Yuval Garini , Nir Katzir , Dirk G. Soeknsen
发明人： Dario Cabib , Robert A. Buckwald , Zvi Malik , Yuval Garini , Nir Katzir , Dirk G. Soeknsen
IPC分类号： G01B11/00 , A61B3/00 , A61B3/12 , A61B5/00 , A61B10/00 , C12Q1/68 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/443 , G01J3/45 , G01J3/453 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/483 , G01N33/50 , G01N33/569 , G01N33/58 , G02B21/36 , G06K9/00 , G06K9/76 , G01B9/02
CPC分类号： G01N21/6458 , C12Q1/6841 , C12Q1/6883 , G01J3/12 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01N21/6428 , G01N21/6486 , G01N33/5005 , G01N33/56966 , G01N33/582 , G06K9/00127 , G06K9/76 , G01J2003/2866 , G01J3/02 , G01N2021/6417 , G01N2021/6423
摘要： According to the present invention there are provided spectral imaging methods for biological research, medical diagnostics and therapy comprising the steps of (a) preparing a sample to be spectrally imaged; (b) viewing the sample through an optical device, the optical device being optically connected to an imaging spectrometer, the optical device and the imaging spectrometer obtaining a spectrum of each pixel of the sample by: (i) collecting incident light simultaneously from all pixels of the sample using collimating optics; (ii) passing the incident collimated light through an interferometer system having a number of elements, to form an exiting light beam; (iii) passing the exiting light beam through a focusing optical system which focuses the exiting light beam on a detector having a two-dimensional array of detector elements, so that at each instant each of the detector elements is the image of one pixel of the sample, so that the real image of the sample is stationary on the plane of the detector array, and so that each of the detector elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths, wherein the linear combination is a function of the instantaneous optical path difference; (iv) rotating one or more of the elements of the interferometer system, so that the optical path difference between the two coherent beams generated by the interferometer system is scanned simultaneously for all the pixels of the sample; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.
摘要翻译：根据本发明，提供了用于生物学研究，医学诊断和治疗的光谱成像方法，其包括以下步骤：（a）制备待成像的样品; （b）通过光学装置观察样品，光学装置光学连接到成像光谱仪，光学装置和成像光谱仪通过以下步骤获得样品的每个像素的光谱：（i）从所有像素同时收集入射光的样品使用准直光学; （ii）使入射的准直光通过具有多个元件的干涉仪系统，以形成出射的光束; （iii）使出射的光束通过聚焦光学系统，该聚焦光学系统将出射的光束聚焦在具有检测器元件的二维阵列的检测器上，使得在每个瞬间，每个检测器元件是样本，使得样本的真实图像在检测器阵列的平面上是静止的，并且使得每个检测器元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合，其中线性组合是瞬时光程差的函数; （iv）旋转干涉仪系统的一个或多个元件，使得由干涉仪系统产生的两个相干光束之间的光程差同时扫描样品的所有像素; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

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