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    • 36. 发明授权
    • Assay
    • 测定
    • US07901949B2
    • 2011-03-08
    • US11423581
    • 2006-06-12
    • Balbir Raj
    • Balbir Raj
    • G01N33/53
    • G01N33/558G01N33/54306G01N33/54386G01N33/54393Y10S435/81
    • The present invention provides an assay kit for detecting an analyte of interest in a sample. The kit comprises a) a reporter species; b) a labelled species having first and second binding regions, wherein the first binding region is capable of binding to the analyte of interest and the second binding region is capable of binding to the reporter species; c) an immobilised species capable of binding to the first binding region of the labelled species; and d) immobilised capture reagent capable of binding to the reporter species. The arrangement is such that the sample is contacted with the labelled species, is then contacted with the immobilised species and is subsequently contacted with the immobilised capture reagent, the reporter species being added prior to exposure of the sample to the immobilised capture reagent. If no analyte is present in the sample, the labelled species becomes bound to the immobilised species and is therefore unable to bind to the immobilised capture reagent. If analyte is present in the sample, the analyte binds to the labelled species such that the labelled species is unable to bind to the immobilised species but can bind to the immobilised capture reagent via the reporter species, the presence of the analyte thus being determined by the presence of labelled species bound to the immobilised capture reagent via the reporter species.
    • 本发明提供了用于检测样品中感兴趣的分析物的测定试剂盒。 该套件包括a)报道物种; b)具有第一和第二结合区域的标记物质,其中所述第一结合区域能够结合目标分析物,并且所述第二结合区域能够结合所述报告物种; c)能够结合标记物种的第一结合区域的固定化物质; 和d)能够结合报告物种的固定捕获试剂。 该装置使得样品与标记物质接触,然后与固定化物质接触,随后与固定的捕获试剂接触,报道物种在将样品暴露于固定的捕获试剂之前加入。 如果样品中没有分析物,则标记物质与固定化物质结合,因此不能与固定的捕获试剂结合。 如果分析物存在于样品中,则分析物结合到标记的物质,使得标记的物质不能结合固定的物质,但是可以通过报道物质与固定的捕获试剂结合,因此分析物的存在由 标记物质通过报道物种与固定的捕获试剂结合的存在。