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    • 31. 发明申请
    • Method for detecting protein
    • 蛋白质检测方法
    • US20050196815A1
    • 2005-09-08
    • US11008954
    • 2004-12-13
    • Yukio Sudo
    • Yukio Sudo
    • G01N33/53C07K16/44C12P21/08G01N33/532G01N33/543G01N33/577G01N33/68G01N33/542
    • G01N33/6845
    • An object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.
    • 本发明的目的是提供一种使用非放射性标记物检测蛋白质的方法,其比生物素/(链霉素)抗生物素蛋白相互作用的情况更小程度地被抑制,并且其检测灵敏度比 检测系统采用DIG。 本发明提供一种检测目标物质的方法,其包括以下步骤:(A)使目标物质与由具有6元环的化合物标记的蛋白质接触,以进行结合反应 ; 和(B)通过使用针对具有6元环的上述化合物的抗体来检测用与目标物质结合的具有6元环的化合物标记的蛋白质。
    • 35. 发明授权
    • Multi-layered element for quantititative analysis of immuno reactant
    • 用于定量分析免疫反应物的多层元件
    • US4975366A
    • 1990-12-04
    • US15916
    • 1987-02-18
    • Yukio SudoNobuhito MasudaKenji Miura
    • Yukio SudoNobuhito MasudaKenji Miura
    • G01N31/22C12Q1/00G01N33/52G01N33/543
    • G01N33/54386G01N33/525Y10S435/805Y10S436/81
    • A multi-layered element for the quantative analysis of an immuno-reactant having a water-impermeable and light-transmissible support layer; a coloring reagent layer carried by said support layer and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix. When the analyte immunoreactant is an antigen, said reaction layer contains the specific antibody for the antigen, the antibody being immobilized by a first water-insoluble carrier. When the analyte immuno-reactant is an antibody, said reaction layer contains the specific antigen for the antibody. The reaction layer contains an enzyme substrate immobilized by a second water-insoluble carrier different from said first water-insoluble carrier. The coloring reagent layer contains a detection reagent composition for coupling with an enzymatic reaction product produced by the reaction beween the enzyme-labelled complex of the immuno-reactant and the enzyme substrate immobilized by the second water-insoluble carrier. Upon coupling of the detection reagent composition with the enzymatic reaction product, a color is developed, the optical density of which is measured to determine the quantity of the analyte immuno-reactant contained in a liquid sample.
    • 用于定量分析具有不透水和透光性支持层的免疫反应物的多层元件; 由所述载体层承载并含有亲水性聚合物作为粘合剂的着色试剂层; 以及覆盖所述试剂层并由多孔基体制成的反应层。 当分析物免疫反应物是抗原时,所述反应层含有针对抗原的特异性抗体,抗体由第一水不溶性载体固定。 当分析物免疫反应物是抗体时,所述反应层含有抗体的特异性抗原。 反应层含有由不同于第一水不溶性载体的第二水不溶性载体固定的酶底物。 着色试剂层含有检测试剂组合物,用于与通过免疫反应物的酶标记的复合物和由第二水不溶性载体固定的酶底物之间的反应产生的酶反应产物偶联。 当检测试剂组合物与酶反应产物偶联时,显色,测量其光密度以确定液体样品中所含的分析物免疫反应物的量。
    • 39. 发明授权
    • Power steering system
    • 动力转向系统
    • US07086494B2
    • 2006-08-08
    • US11104507
    • 2005-04-13
    • Tadaharu YokotaMasakazu KurataMichiya HiramotoYukio Sudo
    • Tadaharu YokotaMasakazu KurataMichiya HiramotoYukio Sudo
    • B62D5/06
    • B62D5/065B62D5/06B62D5/062F15B7/006F15B2211/20561F15B2211/3051
    • A power steering system for a vehicle. The system includes a hydraulic power cylinder, first and second hydraulic lines, a bypass passage through which the first and second hydraulic lines are connected with each other, and a flow passage change-over device adapted to close the bypass passage during steering assisting. A supply passage and a return passage are disposed in parallel with each other to form part of each of the first and second hydraulic lines. The flow passage change-over device is disposed in each of the first and second hydraulic lines. A differential pressure regulating valve is disposed in each of the return passages and adapted to allow hydraulic fluid to flow toward the hydraulic pressure source in a condition where pressure on side of the hydraulic power cylinder becomes higher than a set pressure or higher than pressure on side of the hydraulic pressure source.
    • 一种用于车辆的动力转向系统。 该系统包括液压动力缸,第一和第二液压管路,第一和第二液压管线彼此连接的旁通通道,以及适于在转向辅助期间关闭旁路通道的流路转换装置。 供给通道和返回通道彼此平行地设置以形成第一和第二液压管线中的每一个的一部分。 流路切换装置设置在第一和第二液压管线的每一个中。 差压调节阀设置在每个返回通道中,适于在液压动力缸侧压力高于设定压力或高于侧压力的情况下使液压流体朝向液压源流动 的液压源。