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31. 发明授权

US08796026B2 Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor 有权
标题翻译：从苏云金芽孢杆菌分泌的杀虫蛋白并用于其
公开(公告)号：US08796026B2
公开(公告)日：2014-08-05
申请号：US10563270
申请日：2004-07-06
申请人： James A. Baum , Judith C. Donovan , William P. Donovan , James T. Engleman , Karina Krasomil-Osterfeld , John W. Pitkin , James K. Roberts
发明人： James A. Baum , Judith C. Donovan , William P. Donovan , James T. Engleman , Karina Krasomil-Osterfeld , John W. Pitkin , James K. Roberts
IPC分类号： C12N15/82 , C12N15/32
CPC分类号： A01N63/02 , C07K14/32
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译：本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

32. 发明授权

US07534939B2 Plant transformed with polynucleotide encoding lepidopteran-active Bacillus thuringiensis δ-endotoxin 有权
标题翻译：植物用编码鳞翅目昆虫活性苏云金芽孢杆菌δ-内毒素的多核苷酸转化
公开(公告)号：US07534939B2
公开(公告)日：2009-05-19
申请号：US11487813
申请日：2006-07-17
申请人： James A. Baum , Chih-Rei Chu , William P. Donovan , Amy J. Gilmer , Mark J. Rupar
发明人： James A. Baum , Chih-Rei Chu , William P. Donovan , Amy J. Gilmer , Mark J. Rupar
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译：公开了包含对鳞翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株。还公开了新型苏云金芽孢杆菌基因及其编码的晶体蛋白质，以及制备和使用包含本发明新核酸序列的转基因细胞的方法。

33. 发明申请

US20080229449A1 Lepidopteran-active bacillus thuringiensis delta-endotoxin polynucleotides, compositions, and methods of use 审中-公开
标题翻译：鳞翅目激活型苏云金芽孢杆菌δ-内毒素多核苷酸，组合物和使用方法
公开(公告)号：US20080229449A1
公开(公告)日：2008-09-18
申请号：US12069688
申请日：2008-02-12
申请人： James A. Baum , Chih-Rei Chu , William P. Donovan , Amy J. Gilmer , Mark J. Rupar
发明人： James A. Baum , Chih-Rei Chu , William P. Donovan , Amy J. Gilmer , Mark J. Rupar
IPC分类号： C12N15/11 , C12N15/63 , C12N1/21 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译：公开了包含对鳞翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株。还公开了新型苏云金芽孢杆菌基因及其编码的晶体蛋白质，以及制备和使用包含本发明新核酸序列的转基因细胞的方法。

34. 发明申请

US20080201801A1 Invertebrate MicroRNAs 有权
标题翻译：无脊椎动物微RNA
公开(公告)号：US20080201801A1
公开(公告)日：2008-08-21
申请号：US12033178
申请日：2008-02-19
申请人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
发明人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
IPC分类号： C12N15/11 , A01H5/00
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.
摘要翻译：本发明提供具有抗无脊椎害虫的植物。更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

35. 发明授权

US06949626B2 Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor 有权
标题翻译：苏云金芽孢杆菌cryET33和cryET34组合物及其用途
公开(公告)号：US06949626B2
公开(公告)日：2005-09-27
申请号：US09949972
申请日：2001-09-10
申请人： William P. Donovan , Judith C. Donovan , Annette C. Slaney
发明人： William P. Donovan , Judith C. Donovan , Annette C. Slaney
IPC分类号： A01H5/00 , A01N63/00 , C07K14/325 , C07K16/12 , C12N1/21 , C12N5/02 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/82 , C12P21/02 , C12Q1/68 , C12R1/07 , G01N33/68 , C07K16/00 , G01N33/53
CPC分类号： G01N33/56911 , C07K14/325 , G01N2333/325 , Y10S435/81 , Y10S530/825 , Y10T436/143333
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译：公开了包含对鞘翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株，包括红粉甲虫幼虫（Tribolium castaneum）和日本甲虫幼虫（Popillia japonica）。还公开了新颖的苏云金芽孢杆菌晶体毒素基因，命名为cryET33和cryET34，其编码烯醇毒力晶体蛋白CryET33（29-kDa）晶体蛋白，cryET34基因编码14-kDa CryET34晶体蛋白。 CryET33和CryET34晶体蛋白对红粉甲虫幼虫和日本甲虫幼虫有毒。还公开了制备和使用包含本发明的新核酸序列的转基因细胞的方法。

36. 发明授权

US06593293B1 Lepidopteran-active Bacillus thuringiensis &dgr;-endotoxin compositions and methods of use 有权
标题翻译：鳞翅目活性苏云金芽孢杆菌δ-内毒素的组成及使用方法
公开(公告)号：US06593293B1
公开(公告)日：2003-07-15
申请号：US09661322
申请日：2000-09-13
申请人： James A. Baum , Chih-Rei Chu , William P. Donovan , Amy J. Gilmer , Mark J. Rupar
发明人： James A. Baum , Chih-Rei Chu , William P. Donovan , Amy J. Gilmer , Mark J. Rupar
IPC分类号： A61K3800
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译：公开了包含对鳞翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株。还公开了新型苏云金芽孢杆菌基因及其编码的晶体蛋白质，以及制备和使用包含本发明新核酸序列的转基因细胞的方法。

37. 发明授权

US06399330B1 Bacillus thuringiensis cryet33 and cryet34 compositions and uses thereof 有权
标题翻译：苏云金芽孢杆菌cryet33和cryet34组合物及其用途
公开(公告)号：US06399330B1
公开(公告)日：2002-06-04
申请号：US09549839
申请日：2000-04-14
申请人： William P. Donovan , Judith C. Donovan , Annette C. Slaney
发明人： William P. Donovan , Judith C. Donovan , Annette C. Slaney
IPC分类号： C07H2104
CPC分类号： G01N33/56911 , C07K14/325 , G01N2333/325 , Y10S435/81 , Y10S530/825 , Y10T436/143333
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译：公开了包含对鞘翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株，包括红粉甲虫幼虫（Tribolium castaneum）和日本甲虫幼虫（Popillia japonica）。还公开了新颖的苏云金芽孢杆菌晶体毒素基因，命名为cryET33和cryET34，其编码烯醇毒力晶体蛋白CryET33（29-kDa）晶体蛋白，cryET34基因编码14-kDa CryET34晶体蛋白。 CryET33和CryET34晶体蛋白对红粉甲虫幼虫和日本甲虫幼虫有毒。还公开了制备和使用包含本发明的新核酸序列的转基因细胞的方法。

38. 发明授权

US06326351B1 Bacillus thuringiensis CryET33 and CryET34 compositions and uses therefor 有权
标题翻译：苏云金芽孢杆菌CryET33和CryET34组合物及其用途
公开(公告)号：US06326351B1
公开(公告)日：2001-12-04
申请号：US09147992
申请日：1999-07-21
申请人： William P. Donovan , Judith C. Donovan , Annette C. Slaney
发明人： William P. Donovan , Judith C. Donovan , Annette C. Slaney
IPC分类号： A61K3816
CPC分类号： G01N33/56911 , C07K14/325 , G01N2333/325 , Y10S435/81 , Y10S530/825 , Y10T436/143333
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode respectively the coleopteran-toxic proteins, CryET33 (29-kDa) crystal protein, and CryET34 (14-kDa) crystal protein. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译：公开了包含对鞘翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株，包括红粉甲虫幼虫（Tribolium castaneum）和日本甲虫幼虫（Popillia japonica）。还公开了分别编码鞘翅目毒素蛋白CryET33（29-kDa）晶体蛋白和CryET34（14-kDa）晶体蛋白的新型苏云金芽孢杆菌晶体毒素基因，命名为cryET33和cryET34。还公开了制备和使用包含本发明的新核酸序列的转基因细胞的方法。

39. 发明授权

US5187091A Bacillus thuringiensis cryIIIC gene encoding toxic to coleopteran insects 失效
标题翻译：枸杞子IS IC IC ING ING TS TS TS TS TS TS TS TS TS TS
公开(公告)号：US5187091A
公开(公告)日：1993-02-16
申请号：US496568
申请日：1990-03-20
申请人： William P. Donovan , Mark J. Rupar , Annette C. Slaney , Timothy B. Johnson
发明人： William P. Donovan , Mark J. Rupar , Annette C. Slaney , Timothy B. Johnson
IPC分类号： A01M1/20 , A01N63/00 , A01N63/02 , C07K14/00 , C07K14/195 , C07K14/325 , C07K14/41 , C12N1/20 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/32 , C12P21/00 , C12R1/07
CPC分类号： A01N63/02 , A01N63/00 , C07K14/325 , Y10S530/82
摘要： A purified and isolated cryIII-type gene was obtained from a novel B.t. strain. The gene has a nucleotide base sequence coding for the amino acid sequence illustrated in FIG. 1. The 74.4 kDa protein produced by this gene is an irregularly shaped crystal that is toxic to coleopteran insects, including Colorado potato beetle and insects of the genus Diabrotica.
摘要翻译：从小型B.t.获得纯化和分离的cryIII型基因。应变。该基因具有编码图1所示氨基酸序列的核苷酸碱基序列。 1.该基因产生的74.4 kDa蛋白是一种不规则形状的晶体，对鞘翅目昆虫有毒，包括科罗拉多马铃薯甲虫和Diabrotica属的昆虫。

40. 外观设计

USD310719S Baby bottle 失效
公开(公告)号：USD310719S
公开(公告)日：1990-09-18
申请号：US169769
申请日：1988-03-18
申请人： William P. Donovan
设计人： William P. Donovan

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