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31. 发明授权

US07244622B2 Device and method for multiple analyte detection 有权
标题翻译：多分析物检测的装置和方法
公开(公告)号：US07244622B2
公开(公告)日：2007-07-17
申请号：US11029968
申请日：2005-01-05
申请人： Timothy M. Woudenberg , Michael Albin , Reid B. Kowallis , Yefim Raysberg , Robert P. Ragusa , Emily S. Winn-Deen
发明人： Timothy M. Woudenberg , Michael Albin , Reid B. Kowallis , Yefim Raysberg , Robert P. Ragusa , Emily S. Winn-Deen
IPC分类号： G01N33/543 , G01N15/06 , G01N33/53 , G01N33/566 , G01N21/00 , C12Q1/68 , B32B5/02 , B32B27/12 , B01L3/00 , C12M1/34
CPC分类号： C12Q1/6816 , B01J2219/00317 , B01J2219/00599 , B01J2219/00722 , B01L3/5027 , B01L3/502715 , B01L3/50851 , B01L7/52 , B01L2200/027 , B01L2200/0642 , B01L2300/0816 , B01L2300/0864 , B01L2300/1827 , B01L2400/049 , C12Q1/08 , C40B40/06 , C40B50/08 , C40B60/14 , G01N21/6452 , G01N2035/00148 , Y10S436/805 , Y10S436/807 , Y10S436/808 , Y10S436/809 , Y10T436/143333 , Y10T436/25 , C12Q2565/631
摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
摘要翻译：本发明涉及一种用于同时测试样品中多种所选分析物中一种或多种的存在，不存在和/或量的方法和装置。本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。还公开了利用该装置的方法。

32. 发明授权

US07235406B1 Nucleic acid analysis device 有权
标题翻译：核酸分析装置
公开(公告)号：US07235406B1
公开(公告)日：2007-06-26
申请号：US09628076
申请日：2000-07-28
申请人： Timothy W. Woudenberg , Michael Albin , Reid B. Kowallis , Yefim Raysberg , Robert P. Ragusa , Emily S. Winn-Deen
发明人： Timothy W. Woudenberg , Michael Albin , Reid B. Kowallis , Yefim Raysberg , Robert P. Ragusa , Emily S. Winn-Deen
IPC分类号： G01N33/543 , G01N21/00 , G01N15/06 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/566 , C12Q1/68 , C12M1/34 , B01L3/00 , B32B5/02 , B32B27/12
CPC分类号： G01N21/6452 , B01L3/5027 , B01L7/52 , B01L2200/0642 , B01L2300/0829 , B01L2300/0864 , B01L2300/1827 , B01L2400/049 , G01N21/253 , G01N21/6428 , G01N33/54373 , G01N2021/6432 , Y10S436/807
摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
摘要翻译：本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口。每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。还公开了利用该装置的方法。

33. 发明授权

US6126899A Device for multiple analyte detection 失效
标题翻译：多分析物检测装置
公开(公告)号：US6126899A
公开(公告)日：2000-10-03
申请号：US831983
申请日：1997-04-02
申请人： Timothy W. Woudenberg , Michael Albin , Reid B. Kowallis , Yefim Raysberg , Robert P. Ragusa , Emily S. Winn-Deen
发明人： Timothy W. Woudenberg , Michael Albin , Reid B. Kowallis , Yefim Raysberg , Robert P. Ragusa , Emily S. Winn-Deen
IPC分类号： C12N15/09 , B01L3/00 , B01L7/00 , C12M1/00 , C12Q1/08 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N35/00 , G01N1/14 , G01N15/06 , G01N21/00 , G01N21/64
CPC分类号： G01N33/5304 , B01L3/5027 , B01L3/50273 , C12Q1/08 , B01L2200/027 , B01L2200/0642 , B01L2200/141 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/1827 , B01L2400/049 , B01L7/52 , G01N2035/00148 , Y10S435/973 , Y10S436/807
摘要： The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
摘要翻译：本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口。每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。还公开了利用该装置的方法。

34. 发明授权

US06214191B1 Electrophoresis apparatus and method 失效
标题翻译：电泳装置及方法
公开(公告)号：US06214191B1
公开(公告)日：2001-04-10
申请号：US09448908
申请日：1999-11-23
申请人： John E. Wiktorowicz , Yefim Raysberg
发明人： John E. Wiktorowicz , Yefim Raysberg
IPC分类号： G01N2726
CPC分类号： G01N27/44795 , G01N27/44773
摘要： Disclosed are an apparatus, system, and method for two dimensional electrophoresis of analytes of interest, particularly polypeptides. The apparatus includes a sample separation cavity comprising (1) an electrophoresis region located along an upper portion of the cavity for performing charge and/or size-based electrophoresis in a first dimension along the upper portion, and (2) below the electrophoresis region, a second electrophoresis region for performing electrophoresis in a second dimension in a direction substantially perpendicular to the first dimension. In one preferred embodiment, the second electrophoresis region contains an isoelectric focusing region containing a continuous pKa gradient immobilized on at least one of the major opposing surfaces of the cavity.
摘要翻译：公开了用于感兴趣的分析物，特别是多肽的二维电泳的装置，系统和方法。所述装置包括样品分离腔，所述样品分离腔包括（1）沿所述空腔的上部定位的电泳区，用于沿着所述上部在第一维中进行电荷和/或基于尺寸的电泳，以及（2）所述电泳区以下，第二电泳区域，用于在基本上垂直于第一维度的方向上进行第二维度的电泳。在一个优选的实施方案中，第二电泳区域包含等电聚焦区域，该等电点聚焦区域包含固定在空腔的至少一个主要相对表面上的连续pKa梯度。

35. 发明授权

US6013165A Electrophoresis apparatus and method 失效
标题翻译：电泳装置及方法
公开(公告)号：US6013165A
公开(公告)日：2000-01-11
申请号：US84041
申请日：1998-05-22
申请人： John E. Wiktorowicz , Yefim Raysberg
发明人： John E. Wiktorowicz , Yefim Raysberg
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44795 , G01N27/44773
摘要： Disclosed are an apparatus, system, and method for two dimensional electrophoresis of analytes of interest, particularly polypeptides. The apparatus includes a sample separation cavity comprising (1) an electrophoresis region located along an upper portion of the cavity for performing charge and/or size-based electrophoresis in a first dimension along the upper portion, and (2) below the first said electrophoresis region, a second electrophoresis region for performing electrophoresis in a second dimension in a direction substantially perpendicular to the first dimension. In one preferred embodiment, the second electrophoresis region contains an isoelectric focusing region containing a continuous pKa gradient immobilized on at least one of the major opposing surfaces of the cavity.
摘要翻译：公开了用于感兴趣的分析物，特别是多肽的二维电泳的装置，系统和方法。所述装置包括样品分离腔，所述样品分离腔包括（1）沿所述腔的上部定位的电泳区，用于沿着所述上部在第一维中进行电荷和/或基于尺寸的电泳，以及（2）在所述第一个所述电泳区域，用于在基本上垂直于第一维度的方向上在第二维度中进行电泳的第二电泳区域。在一个优选的实施方案中，第二电泳区域包含等电聚焦区域，该等电点聚焦区域包含固定在空腔的至少一个主要相对表面上的连续pKa梯度。

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