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31. 发明授权

US07776529B2 Methine-substituted cyanine dye compounds 有权
标题翻译：甲基取代的花青染料化合物
公开(公告)号：US07776529B2
公开(公告)日：2010-08-17
申请号：US11005861
申请日：2004-12-06
申请人： Jason Alfred Dallwig , David Carl Hagen , Ching-Ying Cheung , Gerald Alan Thomas , Stephen Yue
发明人： Jason Alfred Dallwig , David Carl Hagen , Ching-Ying Cheung , Gerald Alan Thomas , Stephen Yue
IPC分类号： A61K31/4709 , C07D215/12 , C12Q1/68
CPC分类号： C09B23/164 , C07D277/64 , C07D417/06 , C09B23/0008 , C09B23/04 , C09B23/06 , C09B23/107 , C12Q1/6806 , C12Q1/6876 , C12Q2600/16 , G01N33/5308 , Y10T436/143333
摘要： Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.
摘要翻译：具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

32. 发明申请

US20070268043A1 APPARATUS AND METHODS FOR SELF-BIASING DIFFERENTIAL SIGNALING CIRCUITRY HAVING MULTIMODE OUTPUT CONFIGURATIONS FOR LOW VOLTAGE APPLICATIONS 有权
标题翻译：用于自偏置具有用于低电压应用的多模输出配置的差分信号电路的装置和方法
公开(公告)号：US20070268043A1
公开(公告)日：2007-11-22
申请号：US11830897
申请日：2007-07-31
申请人： Junho Cho , Nancy Chan , Ramesh Senthinathan , Stephen Yue , Richard Fung
发明人： Junho Cho , Nancy Chan , Ramesh Senthinathan , Stephen Yue , Richard Fung
IPC分类号： H03K19/20 , H03K19/094
CPC分类号： H03K17/302 , H03K17/04106
摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.
摘要翻译：本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。两个或更多个开关被包括并配置成根据差分信号电路的操作模式选择性地将电源电压耦合到输出。电路还包括可操作地耦合到至少一个开关和差分信号电路的输出的开关控制偏置电路。开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。此外，体积偏置电路被包括并且可操作地耦合到开关。体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

33. 发明申请

US20060284649A1 APPARATUS AND METHODS FOR SELF-BIASING DIFFERENTIAL SIGNALING CIRCUITRY HAVING MULTIMODE OUTPUT CONFIGURATIONS FOR LOW VOLTAGE APPLICATIONS 有权
标题翻译：用于自偏置具有用于低电压应用的多模输出配置的差分信号电路的装置和方法
公开(公告)号：US20060284649A1
公开(公告)日：2006-12-21
申请号：US11160243
申请日：2005-06-15
申请人： Junho Cho , Nancy Chan , Ramesh Senthinathan , Stephen Yue , Richard Fung
发明人： Junho Cho , Nancy Chan , Ramesh Senthinathan , Stephen Yue , Richard Fung
IPC分类号： H03K19/094
CPC分类号： H03K17/302 , H03K17/04106
摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.
摘要翻译：本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。两个或更多个开关被包括并配置成根据差分信号电路的操作模式选择性地将电源电压耦合到输出。电路还包括可操作地耦合到至少一个开关和差分信号电路的输出的开关控制偏置电路。开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。此外，体积偏置电路被包括并且可操作地耦合到开关。体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

34. 发明授权

US5186769A Seamless steel tube manufacture 失效
标题翻译：无缝钢管制造
公开(公告)号：US5186769A
公开(公告)日：1993-02-16
申请号：US751078
申请日：1991-07-28
申请人： Patrick J. Hunt , John J. Jonas , Stephen Yue , George E. Ruddle , Lakshman N. Pussegoda
发明人： Patrick J. Hunt , John J. Jonas , Stephen Yue , George E. Ruddle , Lakshman N. Pussegoda
IPC分类号： C21D6/02 , C21D8/10
CPC分类号： C21D6/02 , C21D8/10
摘要： A method for producing seamless steel tubes suitable for use as grades of casing and line pipe having yield strengths in excess of 70,000 psi, without being heat treated. Such steels are made of an alloy comprising, by weight, about 0.10% to 0.18% carbon, about 0.10% to 2.0% manganese, about 0.10% to 0.16% vanadium, about 0.008% to 0.012% titanium and about 150 parts per million to 220 parts per million nitrogen, the balance comprising iron and incidental impurities. The subject method comprises the steps of alloying a steel of the aforesaid chemical composition, forming the steel into a billet of steel, reheating the billet in a reheating furnace, passing the billet through a piercing mill to form a steel shell, elongating the steel shell within a mandrel mill, and reducing the diameter of the elongated shell in a stretch reducing mill. Strains are applied to the shell in the stretch reducing mill below the T.sub.nr of the steel and above the A.sub.r3 to provoke dynamic recrystallization. The subject method may also include the steps of cooling the steel shell after it exits the mandrel mill to a temperature below its A.sub.r1 temperature, prior to reheating, and/or accelerated cooling (optional, special cooling may not be necessary for thinner walls) after exiting the stretch reducing mill at a rate between 3.degree. C. to 5.degree. C. per second. The nitrogen and vanadium are preferably introduced to the steel during alloying in the form of a VN alloying agent.
摘要翻译：适用于不经热处理的屈服强度超过70,000psi的套管和管线等级的无缝钢管的生产方法。这种钢由合金组成，包括约0.10％至0.18％的碳，约0.10％至2.0％的锰，约0.10％至0.16％的钒，约0.008％至0.012％的钛和约150ppm的钛 220百万分之氮，余量由铁和附带杂质组成。本发明的方法包括以下步骤：将上述化学组成的钢合金化，将钢形成钢坯，在再加热炉中重新加热坯料，使坯料通过穿孔机形成钢壳，使钢壳伸长在芯棒式无缝管轧机中，并且在减速磨机中减小细长壳体的直径。将菌株施加到钢的Tnr下方的拉伸还原轧机的壳体上，并且在Ar 3上方引起动态再结晶。本发明的方法还可以包括以下步骤：在再次加热和/或加速冷却之后将钢壳冷却到低于其Ar1温度的温度下冷却（可选地，对于较薄壁而言不需要特殊的冷却）以3℃至5℃/秒的速率离开拉伸还原研磨机。氮和钒优选在VN合金化剂形式的合金化过程中引入钢中。

35. 发明授权

US08546580B2 Chemical probe compounds that become fluorescent upon reduction, and methods for their use 有权
公开(公告)号：US08546580B2
公开(公告)日：2013-10-01
申请号：US12908152
申请日：2010-10-20
申请人： Ching-Ying Cheung , Diane Gray , Stephen Yue
发明人： Ching-Ying Cheung , Diane Gray , Stephen Yue
IPC分类号： C07D291/00 , C07C211/00
CPC分类号： G01N33/1806 , C07D211/62 , C07D295/112 , C07D401/14 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/04 , C12Q1/18 , G01N21/643 , G01N33/52 , G01N33/582 , G01N2021/6432
摘要： Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

36. 发明授权

US08415477B2 Violet laser excitable dyes and their method of use 有权
标题翻译：紫激光可激发染料及其使用方法
公开(公告)号：US08415477B2
公开(公告)日：2013-04-09
申请号：US13149392
申请日：2011-05-31
申请人： Gayle Buller , Jixiang Liu , Stephen Yue , Jolene Bradford
发明人： Gayle Buller , Jixiang Liu , Stephen Yue , Jolene Bradford
IPC分类号： C07D413/04 , C07D417/04
CPC分类号： C09B23/0075 , C07D409/04 , C07D413/04 , C07D413/14 , C07D417/04 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/6441
摘要： The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.
摘要翻译：本发明提供了在约400nm处最佳激发并具有至少约80nm的斯托克斯位移的染料化合物。这些染料可用于检测样品中的分析物和染料缀合物的制备。

37. 发明授权

US07871773B2 Aza-benzazolium containing cyanine dyes 有权
标题翻译：含有氰基染料的氮杂 -
公开(公告)号：US07871773B2
公开(公告)日：2011-01-18
申请号：US11756765
申请日：2007-06-01
申请人： Richard Haugland , Stephen Yue
发明人： Richard Haugland , Stephen Yue
IPC分类号： C12Q1/68 , C12Q1/00 , C12Q1/02 , G01N33/53 , C07D215/00 , C07D417/00 , C12P19/34
CPC分类号： C09B23/02 , C12Q1/6816 , C12Q1/6876 , G01N1/30 , G01N27/44726 , G01N33/52 , G01N33/5308 , G01N33/531 , G01N33/582 , G01N33/68 , G01N2001/302 , Y10S436/80 , Y10T436/143333 , C12Q2563/173 , C12Q2563/107
摘要： Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.
摘要翻译：描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

38. 发明申请

US20100227331A1 DETECTION OF IMMOBILIZED NUCLEIC ACID 审中-公开
标题翻译：检测固定化核酸
公开(公告)号：US20100227331A1
公开(公告)日：2010-09-09
申请号：US12778774
申请日：2010-05-12
申请人： Matthew Paul BEAUDET , W. Gregory Cox , Stephen Yue
发明人： Matthew Paul BEAUDET , W. Gregory Cox , Stephen Yue
IPC分类号： C12Q1/68 , C07D401/06
CPC分类号： G01N21/64 , C07D417/06 , C09B23/02 , C09B23/04 , C12Q1/6816 , C12Q1/6825 , G01N1/30 , G01N2001/302 , Y10T436/143333 , C12Q2563/173 , C12Q2565/125
摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.
摘要翻译：本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此确定核酸的存在。花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

39. 发明授权

US07727716B2 Detection of immobilized nucleic acid 有权
标题翻译：检测固定化核酸
公开(公告)号：US07727716B2
公开(公告)日：2010-06-01
申请号：US10956868
申请日：2004-09-30
申请人： Matthew Paul Beaudet , W. Gregory Cox , Stephen Yue
发明人： Matthew Paul Beaudet , W. Gregory Cox , Stephen Yue
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07D209/04
CPC分类号： G01N21/64 , C07D417/06 , C09B23/02 , C09B23/04 , C12Q1/6816 , C12Q1/6825 , G01N1/30 , G01N2001/302 , Y10T436/143333 , C12Q2563/173 , C12Q2565/125
摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.
摘要翻译：本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此确定核酸的存在。花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

40. 发明申请

US20100120051A1 CYANINE DYE COMPOUNDS 有权
标题翻译：氰基染料化合物
公开(公告)号：US20100120051A1
公开(公告)日：2010-05-13
申请号：US12686149
申请日：2010-01-12
申请人： Jason Alfred DALLWIG , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
发明人： Jason Alfred DALLWIG , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
IPC分类号： C12Q1/68
CPC分类号： C09B23/02 , C12Q1/6827 , G01N33/582 , Y10T436/143333 , C12Q2527/125 , C12Q2563/107
摘要： Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, a has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.
摘要翻译：具有带负电荷的取代基的花青染料化合物，其特征在于DNA的荧光染色，包括具有下式的化合物，其中W形成一个或两个稠合的5-或6-元芳环，具有0或1的值，n为0或1，X为O，S或Se，D为吡啶鎓或喹啉鎓部分，条件是该化合物具有至少一个带负电荷的取代基。

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