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31. 发明授权

US07713700B2 Method of amplifying nucleic acids, reagent kit for amplifying nucleic acids, method of detecting single nucleotide polymorphism, and reagent kit for detecting single nucleotide polymorphism 失效
标题翻译：核酸扩增方法，核酸扩增试剂盒，单核苷酸多态性检测方法，单核苷酸多态性检测试剂盒
公开(公告)号：US07713700B2
公开(公告)日：2010-05-11
申请号：US12062215
申请日：2008-04-03
申请人： Yasushi Shigemori , Takehiko Shibata , Tsutomu Mikawa , Michio Oishi , Osamu Ohara
发明人： Yasushi Shigemori , Takehiko Shibata , Tsutomu Mikawa , Michio Oishi , Osamu Ohara
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2521/507
摘要： An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.
摘要翻译：本发明的目的是提供用于扩增所需核酸同时抑制PCR反应中副产物扩增的核酸扩增方法，用于核酸扩增的试剂盒，检测单核苷酸多态性以检测单核苷酸的方法在PCR反应中抑制副产物扩增的多态性，以及用于检测单核苷酸多态性的试剂盒。通过PCR扩增核酸的方法的特征在于在反应溶液中混合含有至少一种源自Thermus thermophilus的RecA蛋白的同源重组蛋白，以及通过修饰RecA蛋白获得的经修饰的RecA蛋白，并且具有功能类似于RecA蛋白，并进行PCR。

32. 发明申请

US20100029702A1 Method For Identification of Compound Having Anti-Anxiety Effect 审中-公开
标题翻译：用于鉴定具有抗焦虑作用的化合物的方法
公开(公告)号：US20100029702A1
公开(公告)日：2010-02-04
申请号：US12447979
申请日：2007-11-01
申请人： Daisuke Okajima , Hiroshi Yokota , Osamu Ohara , Takahiro Nagase , Michio Ohishi
发明人： Daisuke Okajima , Hiroshi Yokota , Osamu Ohara , Takahiro Nagase , Michio Ohishi
IPC分类号： C12Q1/68 , A61K31/438 , A61P25/22
CPC分类号： C12Q1/6883 , A01K2267/0356 , C12Q2600/136 , C12Q2600/156 , G01N2800/301
摘要： There are provided a method for identifying a compound having an anti-anxiety effect, an inhibitor of a function of a protein having a function as a G-protein coupled receptor, characterized by finding the protein and a gene encoding the protein and using at least one of a DNA represented by the nucleotide sequence of this gene or a complementary strand thereof, a homologue of the DNA, a protein encoded by the DNA, and a cell expressing the protein encoded by the DNA, an agent and a method for improving an anxiety condition, a method and an agent for prophylactic and/or therapeutic treatment of an anxiety disorder, and a measurement method for diagnosis and a diagnostic method of an anxiety disorder.
摘要翻译：提供了鉴定具有抗焦虑作用的化合物的方法，具有作为G蛋白偶联受体的功能的蛋白质的功能抑制剂，其特征在于找到蛋白质和编码该蛋白质的基因，并至少使用由该基因的核苷酸序列或其互补链所代表的DNA，DNA的同系物，由DNA编码的蛋白质和表达由DNA编码的蛋白质的细胞之一，药剂和改善其的方法焦虑症，焦虑症的预防和/或治疗方法和药剂，以及焦虑障碍的诊断和诊断方法的测定方法。

33. 发明申请

US20090317304A1 Pipette Core Member, Pipette, and Pipette Device 有权
标题翻译：移液器核心部件，移液器和移液器装置
公开(公告)号：US20090317304A1
公开(公告)日：2009-12-24
申请号：US12515846
申请日：2007-11-22
申请人： Mamoru Hirafuji , Ichiro Tominaga , Shigeto Eda , Ichiro Yanagisawa , Osamu Ohara
发明人： Mamoru Hirafuji , Ichiro Tominaga , Shigeto Eda , Ichiro Yanagisawa , Osamu Ohara
IPC分类号： B01L3/02 , F04F99/00 , C12M1/26
CPC分类号： B01L3/022 , B01L3/0227 , C12M33/04
摘要： A pipette core member is used in a pipette for sampling a sample. This pipette core member includes: a pump body including a reservoir communicated with one suction/discharge port of an electroosmotic flow pump, the electroosmotic flow pump and the reservoir being integrally formed; and a capillary connected to the electroosmotic flow pump and communicated with the other suction/discharge port of the electroosmotic flow pump. The capillary is secured to the pump body.
摘要翻译：在移液管中使用移液管芯构件来取样。该吸管芯构件包括：泵主体，包括与电渗流泵的一个吸入/排出口连通的储存器，电渗流量泵和储存器整体形成; 以及连接到电渗流量泵并与电渗流量泵的另一吸入/排出口连通的毛细管。毛细管固定在泵体上。

34. 发明授权

US07482322B2 Plexin family-like polypeptide, and uses thereof 失效
标题翻译：丛状家族样多肽及其用途
公开(公告)号：US07482322B2
公开(公告)日：2009-01-27
申请号：US10573262
申请日：2004-10-28
申请人： Hisashi Koga , Osamu Ohara , Haruhiko Koseki , Mitsuhiro Okada , Akiyoshi Uemura , Hiroki Arakawa , Mitsuhiro Tada
发明人： Hisashi Koga , Osamu Ohara , Haruhiko Koseki , Mitsuhiro Okada , Akiyoshi Uemura , Hiroki Arakawa , Mitsuhiro Tada
IPC分类号： A61K38/16 , A61K38/00 , C07K14/435 , C07K14/00
CPC分类号： C07K14/705 , A61K38/00
摘要： The present invention is directed to a novel plexin family like DNA, a gene containing the DNA, novel polypeptide encoded by the DNA, a recombinant protein containing the polypeptide or heterologous polypeptide sequences, antibodies which bind to the polypeptides, methods for producing the polypeptides of the present invention. Conditions that can be diagnosed, screened, prevented, or treated by the compositions herein include prophylactic, angiogenesis and conservation of functions in various organs, angiogenesis at the time of tumor and regeneration of various organs, proliferation, differentiation and preservation of function of the cells in various organs, aging, and dysfunction of the present invention.
摘要翻译：本发明涉及一种新型的plexin家族如DNA，含有该DNA的基因，由该DNA编码的新型多肽，含有该多肽或异源多肽序列的重组蛋白，与该多肽结合的抗体，产生多肽的多肽的方法本发明。可以通过本文组合物诊断，筛选，预防或治疗的病症包括各种器官的功能的预防，血管生成和保护，肿瘤时的血管发生和各种器官的再生，细胞的功能的增殖，分化和保存在本发明的各种器官，衰老和功能障碍中。

35. 发明申请

US20080274954A1 Gene Encoding a Guanine Nucleotide Exchange Factor and Its Gene Product 审中-公开
标题翻译：基因编码鸟嘌呤核苷酸交换因子及其基因产物
公开(公告)号：US20080274954A1
公开(公告)日：2008-11-06
申请号：US11578324
申请日：2005-04-14
申请人： Osamu Ohara , Takahiro Nagase , Michio Ohishi , Hiroshi Yokota , Osamu Kamida
发明人： Osamu Ohara , Takahiro Nagase , Michio Ohishi , Hiroshi Yokota , Osamu Kamida
IPC分类号： A61K38/00 , C12N15/11 , C12N15/00 , C12N5/06 , C07K16/18 , A61P31/00 , C12P21/04 , G01N33/00 , C12Q1/68
CPC分类号： G01N33/57446 , A61K38/00 , C07K14/82 , C12Q1/44 , G01N33/57407 , G01N2500/00 , Y10T436/143333
摘要： A gene encoding a novel protein having a guanine nucleotide exchange factor (GEF) activity for a Rho family protein being one group of small GTP binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.
摘要翻译：编码具有Rho家族蛋白质的鸟嘌呤核苷酸交换因子（GEF）活性的新蛋白质的基因是一组小GTP结合蛋白，即由SEQ ID NO：1，SEQ ID NO：1所示的核苷酸序列所示的多核苷酸 SEQ ID NO：3或SEQ ID NO：5，或互补链，多核苷酸的等同物，由多核苷酸编码的蛋白质，含有多核苷酸的载体，含有该载体的转化体，由该多核苷酸编码的蛋白质的抗体提供了鉴定抑制多核苷酸编码的蛋白质的功能和/或多核苷酸表达的化合物的方法，确定疾病的方法，药物组合物和试剂盒的方法。

36. 发明授权

US07344838B2 Gene encoding brain specific membrane protein 失效
标题翻译：基因编码脑特异性膜蛋白
公开(公告)号：US07344838B2
公开(公告)日：2008-03-18
申请号：US11007517
申请日：2004-12-07
申请人： Shin-Ichi Funahashi , Shoji Miyata , Nobuo Nomura , Takahiro Nagase , Osamu Ohara
发明人： Shin-Ichi Funahashi , Shoji Miyata , Nobuo Nomura , Takahiro Nagase , Osamu Ohara
IPC分类号： C12Q1/68 , C12P21/02 , C12N5/10 , C12N15/63 , C07H21/04
CPC分类号： C07K14/705
摘要： A novel gene apparently encoding a transmembrane glycoprotein has been successfully isolated by constructing a cDNA library of 4 kb or above in size from mRNA expressed in human adult brain and analyzing the structures of the cDNAs contained within said library by the shotgun method. The novel gene shows brain-specific expression and the protein encoded by said gene has a typical PDZ binding motif.
摘要翻译：通过构建人大脑中表达的mRNA的大小为4kb或以上的cDNA文库，并通过霰弹枪法分析所述文库中所含的cDNA的结构，已经成功地分离了显然编码跨膜糖蛋白的新基因。该新基因显示脑特异性表达，由该基因编码的蛋白具有典型的PDZ结合基序。

37. 发明申请

US20070212365A1 Novel Plexin Polypeptide, Dna Encoding the Same and Use Thereof 失效
标题翻译：新型Plexin多肽，Dna编码相同，并使用它们
公开(公告)号：US20070212365A1
公开(公告)日：2007-09-13
申请号：US10573262
申请日：2004-10-28
申请人： Hisashi Koga , Osamu Ohara , Haruhiko Koseki , Mitsuhiro Okada , Akiyoshi Uemura , Hiroki Arakawa , Mitsuhiro Tada
发明人： Hisashi Koga , Osamu Ohara , Haruhiko Koseki , Mitsuhiro Okada , Akiyoshi Uemura , Hiroki Arakawa , Mitsuhiro Tada
IPC分类号： A61K39/00 , G01N33/574 , C07H21/04 , C12P21/06 , C12N5/06 , C07K14/82
CPC分类号： C07K14/705 , A61K38/00
摘要： The object of the invention is to provide novel DNA, a gene containing the DNA, novel polypeptide encoded by the DNA, a recombinant protein containing the polypeptide, or an antibody against the polypeptide, which is useful for development and screening of prophylactic, therapeutic and diagnostic drug for angiogenesis and conservation of functions in various organs, angiogenesis at the time of tumor and regeneration of various organs, proliferation, differentiation and preservation of function of the cells in various organs, aging, and dysfunction. Means to Solve the Problem A DNA comprising a base sequence encoding one of the following polypeptide (a) or (b): (a) a polypeptide comprising the full length or a part of an amino acid sequence which is the same or substantially the same as an amino acid sequence represented by SEQ ID NO:1, SEQ ID NO:15 or SEQ ID NO:18, and (b) a polypeptide comprising an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO:1, SEQ ID NO:15 or SEQ ID NO:18 by deletion, substitution or addition of a part of the amino acids and having a biological activity substantially equivalent to the polypeptide (a).
摘要翻译：本发明的目的是提供新的DNA，含有DNA的基因，由DNA编码的新型多肽，含有多肽的重组蛋白或抗多肽的抗体，其可用于开发和筛选预防性治疗和用于血管发生的诊断药物和各种器官功能的保存，肿瘤时的血管发生和各种器官的再生，增殖，分化和保存各种器官，衰老和功能障碍中的细胞的功能。解决问题的方法包含编码以下多肽（a）或（b）之一的碱基序列的DNA：（a）包含相同或基本相同的氨基酸序列的全长或部分的多肽作为由SEQ ID NO：1，SEQ ID NO：15或SEQ ID NO：18表示的氨基酸序列，和（b）包含源自SEQ ID NO：1所示氨基酸序列的氨基酸序列的多肽， SEQ ID NO：15或SEQ ID NO：18，通过缺失，取代或添加一部分氨基酸并且具有与多肽（a）基本相同的生物活性。

38. 发明申请

US20070196377A1 Gene encoding a guanmine nucleotide exchange factor and the gene product thereof 失效
标题翻译：基因编码鸟苷核苷酸交换因子及其基因产物
公开(公告)号：US20070196377A1
公开(公告)日：2007-08-23
申请号：US10594707
申请日：2005-03-29
申请人： Osamu Ohara , Takahiro Nagase , Michi Oishi , Hiroshi Yokota , Osamu Kamida
发明人： Osamu Ohara , Takahiro Nagase , Michi Oishi , Hiroshi Yokota , Osamu Kamida
IPC分类号： A61K48/00 , C12Q1/68 , G01N33/574 , C07H21/04 , C07K16/40 , A61K39/395 , C12P21/06 , C12N9/10
CPC分类号： A61K31/7088 , C07K14/4702 , C07K14/82 , G01N33/57446 , G01N33/57484 , G01N2333/4703 , Y10S435/81 , Y10S435/975
摘要： A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.
摘要翻译：编码作为Rho家族蛋白质的鸟嘌呤核苷酸交换因子（GEF）的新型蛋白质的基因是一组小的GTP结合蛋白，即由SEQ ID NO：1所示的核苷酸序列显示的多核苷酸， SEQ ID NO：3或SEQ ID NO：5，或互补链，多核苷酸的等同物，由多核苷酸编码的蛋白质，含有多核苷酸的载体，含有该载体的转化体，提供多核苷酸，鉴定抑制多核苷酸编码的蛋白质的功能的化合物的方法和/或多核苷酸的表达，确定疾病的方法，药物组合物和试剂盒的方法。

39. 发明申请

US20070128645A1 Use of KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development 审中-公开
标题翻译：使用KIAA0172基因治疗和诊断疾病以及药物开发
公开(公告)号：US20070128645A1
公开(公告)日：2007-06-07
申请号：US11606148
申请日：2006-11-30
申请人： Ryoichi Kiyama , Keisuke Kitajima , Shinobu Oguchi , Michio Oishi , Osamu Ohara , Takahiro Nagase
发明人： Ryoichi Kiyama , Keisuke Kitajima , Shinobu Oguchi , Michio Oishi , Osamu Ohara , Takahiro Nagase
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6886 , C07K14/47 , C12Q2600/154 , C12Q2600/172 , G01N33/505
摘要： An object of the present invention is to provide use of the KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development. An agent for treating cancer which comprises as an active ingredient a polypeptide encoded by the KIAA0172 gene, a partial sequence thereof or a variant thereof; an agent for treating cancer which comprises as an active ingredient an oligonucleotide including the KIAA0172 gene sequence; an agent for detecting cancer which comprises an antibody which recognizes polypeptide encoded by the KIAA0172 gene; an agent for detecting cancer which comprises an oligonucleotide including the KIAA0172 gene sequence; a composition for treating cancer which comprises said agent for treating cancer and a pharmaceutically acceptable carrier; and a composition for detecting cancer which comprises said agent for detecting cancer and a pharmaceutically acceptable carrier.
摘要翻译：本发明的目的是提供KIAA0172基因在疾病和药物开发的治疗和诊断中的用途。一种用于治疗癌症的药物，其包含作为活性成分的由KIAA0172基因编码的多肽，其部分序列或其变体; 一种治疗癌症的药物，其包含作为活性成分的包含KIAA0172基因序列的寡核苷酸; 用于检测癌症的试剂，其包含识别由KIAA0172基因编码的多肽的抗体; 用于检测癌症的试剂，其包含含有KIAA0172基因序列的寡核苷酸; 用于治疗癌症的组合物，其包含所述治疗癌症的药物和药学上可接受的载体; 和用于检测癌症的组合物，其包含所述用于检测癌症的药物和药学上可接受的载体。

40. 发明申请

US20050106719A1 Method of efficiently recombining orf encoded by cdna and template vector, trap vector and primer to be used therein 审中-公开
标题翻译：由cdna和模板载体有效重组或编码的方法，其中使用的捕获载体和引物
公开(公告)号：US20050106719A1
公开(公告)日：2005-05-19
申请号：US10491192
申请日：2003-12-04
申请人： Takahiro Nagase , Daisuke Nakajima , Osamu Ohara
发明人： Takahiro Nagase , Daisuke Nakajima , Osamu Ohara
IPC分类号： C12N15/10 , C12N15/64 , C12N15/66 , C12N15/85 , C12N15/74
CPC分类号： C12N15/66 , C12N15/64
摘要： The object of the present invention is to solve the problems in excising a DNA region (or gene) with conventional restriction enzymes, or in amplification of the DNA region by PCR. The present invention provides a method for precisely, quickly and simply cloning a target ORF, for example the target ORF encoding a protein, particularly the target ORF encoding a long-chain ORF comprising bases as many as several thousand bp in cDNA, and means such as various vectors used for the cloning method,
摘要翻译：本发明的目的是解决用常规限制酶切除DNA区域（或基因）或通过PCR扩增DNA区域的问题。本发明提供了精确，快速和简单地克隆靶ORF的方法，例如编码蛋白质的靶ORF，特别是编码包含cDNA多达数千bp的碱基的长链ORF的靶ORF，作为用于克隆方法的各种载体，

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