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31. 发明授权

US07951553B2 Method of assaying glycated protein 有权
标题翻译：测定糖化蛋白的方法
公开(公告)号：US07951553B2
公开(公告)日：2011-05-31
申请号：US10580000
申请日：2004-11-18
申请人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
发明人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
IPC分类号： C12Q1/37
CPC分类号： G01N33/726 , G01N33/6812 , G01N2333/95
摘要： The present invention provides a convenient, efficient method for assaying glycated protein, fructosyl peptide, or fructosyl amino acid which can be performed with reduced effect of fructosyl lysine compounds. The invention also provides a reagent for the assay.The invention is directed to a method for reducing the effect of a fructosyl lysine compound in an assay of fructosyl peptide or fructosyl amino acid contained in a sample, characterized by including causing an enzyme for assaying fructosyl peptide or fructosyl amino acid to act specifically on fructosyl peptide or fructosyl amino acid at a pH of 4.0 to 7.0 and measuring the product at a pH of 4.0 to 7.0; and a method for assaying glycated protein through the above method.
摘要翻译：本发明提供了一种用于测定糖基化蛋白质，果糖基肽或果糖基氨基酸的方便，有效的方法，其可以降低果糖基赖氨酸化合物的作用。本发明还提供了用于测定的试剂。本发明涉及一种降低果糖基赖氨酸化合物在样品中含有的果糖基肽或果糖基氨基酸的影响的方法，其特征在于包括使酶用于测定果糖基肽或果糖基氨基酸以特异性地作用于果糖基肽或果糖基氨基酸，pH为4.0〜7.0，在pH7.0〜7.0下测定产物; 以及通过上述方法测定糖化蛋白质的方法。

32. 发明授权

US07407774B2 Method for reducing influence of hemoglobin with albumin in an assay 有权
标题翻译：在测定中减少血红蛋白与白蛋白的影响的方法
公开(公告)号：US07407774B2
公开(公告)日：2008-08-05
申请号：US11684827
申请日：2007-03-12
申请人： Hiroyuki Ebinuma , Kumiko Yuki
发明人： Hiroyuki Ebinuma , Kumiko Yuki
IPC分类号： C12Q1/26
CPC分类号： C12Q1/32
摘要： A method for quantitatively determining a specific component in a biological specimen, which includes reacting a biological specimen, in the presence of an electron acceptor, with an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component, and measuring the formed reductant of the electron acceptor, wherein the method avoids the influence of hemoglobin effectively if any contained in the specimen by using a measuring reagent containing albumin, thereby making an quantitative determination of the target component accurately; and a reagent for the quantitative determination.
摘要翻译：一种用于定量测定生物样本中的特定成分的方法，其包括使生物样品在电子受体存在下与通过脱氢反应具有能够氧化特定成分或衍生物质的酶反应的酶测定形成的电子受体的还原剂，其中，通过使用含有白蛋白的测定试剂，如果含有试样中的任何含量，则可有效地避免血红蛋白的影响，从而准确地定量确定目标成分; 和用于定量测定的试剂。

33. 发明授权

US07393549B2 Defructosylation method 失效
标题翻译：脱甲硅烷基化方法
公开(公告)号：US07393549B2
公开(公告)日：2008-07-01
申请号：US10531321
申请日：2003-10-23
申请人： Hiroyuki Ebinuma
发明人： Hiroyuki Ebinuma
IPC分类号： A01N65/00 , C12P21/06
CPC分类号： C07K14/805 , C12N9/0022 , C12N9/2402 , C12P21/00 , C12P21/005 , C12Q1/26 , C12Q1/34 , G01N33/68 , G01N33/725 , G01N2400/00
摘要： The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein.
摘要翻译：本发明涉及来自植物的脱甲硅烷基化酶，通过使用酶使果糖基化肽或蛋白质去结构化的方法，以及测量果糖基化肽或蛋白质的方法。

34. 发明申请

US20070065891A1 Method of pretreating sample and immunological assay method using the same 有权
标题翻译：预处理样品的方法和使用其的免疫测定方法
公开(公告)号：US20070065891A1
公开(公告)日：2007-03-22
申请号：US10575711
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
IPC分类号： G01N33/569 , G01N33/554
CPC分类号： G01N33/74 , G01N2333/72 , Y10T436/25
摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers. The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.
摘要翻译：本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。

35. 发明申请

US20060072040A1 Video signal processing circuit 有权
标题翻译：视频信号处理电路
公开(公告)号：US20060072040A1
公开(公告)日：2006-04-06
申请号：US11242772
申请日：2005-10-04
申请人： Ikuo Osawa , Yoshifumi Yoshida , Hiroyuki Ebinuma , Toru Okada
发明人： Ikuo Osawa , Yoshifumi Yoshida , Hiroyuki Ebinuma , Toru Okada
IPC分类号： H03M1/12
CPC分类号： H04N5/14 , H04N5/08 , H04N5/126 , H04N9/64
摘要： A video signal processing circuit is supplied with an analog composite video signal formed by combining at least a luminance signal with a sync signal, and processes the analog composite video signal. The video signal processing circuit has an analog filter which removes high-frequency components from the analog composite video signal, a sync separation circuit which separates a sync signal from an output signal from the analog filter, an AD converter which performs AD conversion on the analog composite video signal, and a digital video signal processing circuit which performs predetermined video signal processing on the composite video signal digitized by the AD converter, by using the sync signal obtained by the sync separation circuit.
摘要翻译：视频信号处理电路被提供有通过将至少亮度信号与同步信号组合而形成的模拟复合视频信号，并处理模拟复合视频信号。视频信号处理电路具有从模拟复合视频信号中去除高频分量的模拟滤波器，将同步信号与来自模拟滤波器的输出信号分离的同步分离电路，对模拟信号进行AD转换的AD转换器复合视频信号和数字视频信号处理电路，通过使用由同步分离电路获得的同步信号，对由AD转换器数字化的复合视频信号执行预定的视频信号处理。

36. 发明授权

US06969613B1 Method for determination of hydrogen sulfide or sulfide ion and method for determination of specific substance utilizing said method 有权
标题翻译：用于测定硫化氢或硫化物离子的方法和利用所述方法测定特定物质的方法
公开(公告)号：US06969613B1
公开(公告)日：2005-11-29
申请号：US09856790
申请日：1999-12-07
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
IPC分类号： C12Q1/527 , G01N31/22 , G01N33/00
CPC分类号： C12Q1/527 , G01N31/22 , G01N31/224 , G01N33/0044 , Y10T436/18 , Y10T436/182
摘要： The present invention provides a method for quantitatively determining hydrogen sulfide or sulfide ions conveniently with high sensitivity, which comprises adding to a sample containing hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator. The present invention further provides a method for quantitatively determining a specific substance, which comprises adding to a sample containing a specific substance, a component which acts on the specific substance so that the specific substance forms hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions, and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator.
摘要翻译：本发明提供了一种以高灵敏度方便地定量测定硫化氢或硫化物离子的方法，其包括向含有硫化氢或硫化物离子的金属离子或释放所述金属离子的化合物和与金属离子发生显色，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。本发明还提供了一种定量测定特定物质的方法，该方法包括向含有特定物质的样品中加入作用于特定物质的成分，使特定物质形成硫化氢或硫化物离子，金属离子或化合物释放出所述金属离子的金属指示剂和与金属离子反应并导致发色的金属指示剂，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。

37. 发明授权

US07608405B2 Method of selectively assaying adiponectin multimers 有权
标题翻译：选择性测定脂连蛋白多聚体的方法
公开(公告)号：US07608405B2
公开(公告)日：2009-10-27
申请号：US10575931
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
IPC分类号： G01N33/53 , G01N33/543 , A61K39/395
CPC分类号： G01N33/74 , G01N2800/042 , G01N2800/32
摘要： Methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying of a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.
摘要翻译：用于选择性测定生物样品中目标脂联素多聚体的方法。这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其他脂联素多聚体区分开。

38. 发明申请

US20090096928A1 COLOR KILLER CIRCUIT 有权
标题翻译：彩色杀手电路
公开(公告)号：US20090096928A1
公开(公告)日：2009-04-16
申请号：US12249489
申请日：2008-10-10
申请人： Chikara Sakyo , Hiroyuki Ebinuma
发明人： Chikara Sakyo , Hiroyuki Ebinuma
IPC分类号： H04N9/70
CPC分类号： H04N9/70
摘要： There is provided a color killer circuit comprising a burst amplitude detection unit that detects an amplitude of a color burst; a delay unit that stores, delays, by a predetermined period of time, and outputs the detected amplitude; a subtractor that calculates a difference between an output from the burst amplitude detection unit and an output from the delay unit; a first comparator that compares the output from the burst amplitude detection unit with a first threshold value to determine whether or not the output from the burst amplitude detection unit is less than the first threshold value; and a second comparator that compares an output from the subtractor with a second threshold value to determine whether or not the output from the subtractor is greater than the second threshold value, wherein the color killer circuit masks the color component of the composite video signal when the first comparator determines that the output from the burst amplitude detection unit is less than the first threshold value, or when the second comparator determines that the output from the subtractor is greater than the second threshold value.
摘要翻译：提供了一种颜色抑制电路，其包括检测彩色脉冲的幅度的突发幅度检测单元; 延迟单元，其在预定时间段内存储，延迟并输出检测到的幅度; 计算来自所述脉冲串幅度检测单元的输出与所述延迟单元的输出之间的差的减法器; 第一比较器，将来自脉冲串幅度检测单元的输出与第一阈值进行比较，以确定来自脉冲串幅度检测单元的输出是否小于第一阈值; 以及第二比较器，其将来自所述减法器的输出与第二阈值进行比较，以确定所述减法器的输出是否大于所述第二阈值，其中，当所述复合视频信号的颜色分量在第一比较器确定来自脉冲串幅度检测单元的输出小于第一阈值，或者当第二比较器确定来自减法器的输出大于第二阈值时。

39. 发明授权

US07456903B2 Video signal processing circuit 有权
标题翻译：视频信号处理电路
公开(公告)号：US07456903B2
公开(公告)日：2008-11-25
申请号：US11242772
申请日：2005-10-04
申请人： Ikuo Osawa , Yoshifumi Yoshida , Hiroyuki Ebinuma , Toru Okada
发明人： Ikuo Osawa , Yoshifumi Yoshida , Hiroyuki Ebinuma , Toru Okada
IPC分类号： H04N9/64 , H04N5/08
CPC分类号： H04N5/14 , H04N5/08 , H04N5/126 , H04N9/64
摘要： A video signal processing circuit is supplied with an analog composite video signal formed by combining at least a luminance signal with a sync signal, and processes the analog composite video signal. The video signal processing circuit has an analog filter which removes high-frequency components from the analog composite video signal, a sync separation circuit which separates a sync signal from an output signal from the analog filter, an AD converter which performs AD conversion on the analog composite video signal, and a digital video signal processing circuit which performs predetermined video signal processing on the composite video signal digitized by the AD converter, by using the sync signal obtained by the sync separation circuit.
摘要翻译：视频信号处理电路被提供有通过将至少亮度信号与同步信号组合而形成的模拟复合视频信号，并处理模拟复合视频信号。视频信号处理电路具有从模拟复合视频信号中去除高频分量的模拟滤波器，将同步信号与来自模拟滤波器的输出信号分离的同步分离电路，对模拟信号进行AD转换的AD转换器复合视频信号和数字视频信号处理电路，通过使用由同步分离电路获得的同步信号，对由AD转换器数字化的复合视频信号执行预定的视频信号处理。

40. 发明申请

US20080233605A1 Method of Assaying Glycated Protein 有权
标题翻译：测定糖化蛋白的方法
公开(公告)号：US20080233605A1
公开(公告)日：2008-09-25
申请号：US10580000
申请日：2004-11-18
申请人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
发明人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
IPC分类号： C12Q1/37 , C12Q1/00
CPC分类号： G01N33/726 , G01N33/6812 , G01N2333/95
摘要： The present invention provides a convenient, efficient method for assaying glycated protein, fructosyl peptide, or fructosyl amino acid which can be performed with reduced effect of fructosyl lysine compounds. The invention also provides a reagent for the assay.The invention is directed to a method for reducing the effect of a fructosyl lysine compound in an assay of fructosyl peptide or fructosyl amino acid contained in a sample, characterized by including causing an enzyme for assaying fructosyl peptide or fructosyl amino acid to act specifically on fructosyl peptide or fructosyl amino acid at a pH of 4.0 to 7.0 and measuring the product at a pH of 4.0 to 7.0; and a method for assaying glycated protein through the above method.
摘要翻译：本发明提供了一种用于测定糖基化蛋白质，果糖基肽或果糖基氨基酸的方便，有效的方法，其可以降低果糖基赖氨酸化合物的作用。本发明还提供了用于测定的试剂。本发明涉及一种降低果糖基赖氨酸化合物在样品中含有的果糖基肽或果糖基氨基酸的影响的方法，其特征在于包括使酶用于测定果糖基肽或果糖基氨基酸以特异性地作用于果糖基肽或果糖基氨基酸，pH为4.0〜7.0，在pH7.0〜7.0下测定产物; 以及通过上述方法测定糖化蛋白质的方法。

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