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31. 发明申请

US20060121560A1 DNA fragment to promote translation reaction and method for cell-free protein synthesis system using the same 审中-公开
标题翻译： DNA片段促进翻译反应和方法，使用无细胞蛋白质合成系统
公开(公告)号：US20060121560A1
公开(公告)日：2006-06-08
申请号：US11295739
申请日：2005-12-07
申请人： Takashi Suzuki , Masaaki Ito , Toru Ezure , Masamitsu Shikata , Shinichiro Kobayashi
发明人： Takashi Suzuki , Masaaki Ito , Toru Ezure , Masamitsu Shikata , Shinichiro Kobayashi
IPC分类号： C12P21/06 , C12N5/06 , C12N15/63
CPC分类号： C12P21/00 , C07K14/43586
摘要： The present invention provides a DNA fragment allowing easy cloning of a desired gene and capable of further improving translation efficiency, a protein expression vector and a template DNA having the DNA fragment, a mRNA obtained from the template DNA, a reaction solution for cell-free protein synthesis system containing the template DNA or the mRNA, a method for cell-free protein synthesis system using the template DNA, and, kit for cell-free protein synthesis system including the expression vector. A DNA fragment having the base sequence represented by any of SEQ ID No. 1 to 11 to use for promoting translation reaction, a protein expression vector and a template DNA having the DNA fragment, a mRNA obtained from the template DNA, a reaction solution for cell-free protein synthesis system containing the template DNA or the mRNA, a method for cell-free protein synthesis system using the template DNA, and, kit for cell-free protein synthesis system including the expression vector.
摘要翻译：本发明提供了能够容易地克隆所需基因并能够进一步提高翻译效率的DNA片段，蛋白质表达载体和具有DNA片段的模板DNA，从模板DNA获得的mRNA，无细胞的反应溶液含有模板DNA或mRNA的蛋白质合成系统，使用模板DNA的无细胞蛋白质合成系统的方法，以及包含表达载体的无细胞蛋白质合成系统的试剂盒。具有用于促进翻译反应的SEQ ID No.1至11所示的碱基序列的DNA片段，具有该DNA片段的蛋白质表达载体和模板DNA，从模板DNA获得的mRNA，含有模板DNA或mRNA的无细胞蛋白质合成系统，使用模板DNA的无细胞蛋白质合成系统的方法，以及包含表达载体的无细胞蛋白质合成系统的试剂盒。

32. 发明申请

US20060084146A1 Method for cell-free protein synthesis using extract solution derived from insect cell 有权
标题翻译：使用衍生自昆虫细胞的提取物溶液进行无细胞蛋白质合成的方法
公开(公告)号：US20060084146A1
公开(公告)日：2006-04-20
申请号：US11247294
申请日：2005-10-12
申请人： Masamitsu Shikata , Nobuhiro Hanafusa , Shinichiro Kobayashi
发明人： Masamitsu Shikata , Nobuhiro Hanafusa , Shinichiro Kobayashi
IPC分类号： C12P21/06 , C12N5/06
CPC分类号： C12P21/00
摘要： The present invention provides a simple cell-free protein synthesis method capable of affording synthesis of a protein in a high amount in a short time at a low cost. A method for cell-free protein synthesis using an extract derived from an insect cell, the method comprising removing a component which can pass through a semipermeable membrane through the semipermeable membrane while maintaining synthesis reaction, thereby to continuously synthesize a protein. Preferably, an mRNA is additionally supplied while said synthesis reaction is maintained. Further, said insect cell is preferably an established culture cell derived from Trichoplusia ni ovum cell.
摘要翻译：本发明提供了一种简单的无细胞蛋白质合成方法，其能够以低成本在短时间内高效地提供蛋白质的合成。一种使用来自昆虫细胞的提取物进行无细胞蛋白质合成的方法，该方法包括除去可以通过半透膜的半透膜的成分，同时保持合成反应，从而连续合成蛋白质。优选地，在保持所述合成反应的同时提供mRNA。此外，所述昆虫细胞优选是源自粉纹夜蛾卵母细胞的建立的培养细胞。

33. 发明授权

US06565952B1 Ink-jet recording sheet 失效
标题翻译：喷墨记录纸
公开(公告)号：US06565952B1
公开(公告)日：2003-05-20
申请号：US09720989
申请日：2001-01-03
申请人： Takayuki Ishikawa , Shinichiro Kobayashi , Takeshi Yoshimoto , Nobuhiro Kubota , Kiyoshi Iwamoto , Jun Sugiyama , Hiroyuki Onishi , Yukari Sano
发明人： Takayuki Ishikawa , Shinichiro Kobayashi , Takeshi Yoshimoto , Nobuhiro Kubota , Kiyoshi Iwamoto , Jun Sugiyama , Hiroyuki Onishi , Yukari Sano
IPC分类号： B32B300
CPC分类号： B41M5/5254 , B41M5/52 , B41M5/5218 , Y10T428/31935 , Y10T428/31938
摘要： The invention provides an ink-jet recording sheet having an excellent preventive effect on overall. degradation by light such as degradation of image clarity, and decrease in density or change of tone of images, and a resin composition for ink-jet recording media used in the formation thereof. The recording sheet of the invention comprises a substrate and as an ink-receiving layer provided on the substrate, a layer containing an acrylic resin comprising, as copolymerizable monomers, (a) a dialkylaminoalkyl methacrylate, (b) acrylamide and (c) at least one monomer selected from among an. alkoxypolyethylene glycol methacrylate, a 2-hydroxyalkyl methacrylate and a 2-hydroxyalkyl acrylate.
摘要翻译：本发明提供一种喷墨记录纸，其总体上具有优异的预防效果。通过光降解，例如图像清晰度的降低，图像的浓度或色调变化的降低，以及用于其形成的喷墨记录介质的树脂组合物。本发明的记录纸包括基材和设置在基材上的油墨接收层，含有丙烯酸树脂的层包含作为可共聚单体的（a）甲基丙烯酸二烷基氨基烷基酯，（b）丙烯酰胺和（c）至少一种选自以下的单体。烷氧基聚乙二醇甲基丙烯酸酯，2-羟烷基甲基丙烯酸酯和2-羟基烷基丙烯酸酯。

34. 发明授权

US06513269B2 Steam sprayer 失效
标题翻译：蒸汽喷雾器
公开(公告)号：US06513269B2
公开(公告)日：2003-02-04
申请号：US09912320
申请日：2001-07-26
申请人： Shinichiro Kobayashi , Toshihide Nakamura , Mamoru Ikeshima , Masao Shimizu
发明人： Shinichiro Kobayashi , Toshihide Nakamura , Mamoru Ikeshima , Masao Shimizu
IPC分类号： D06F7520
CPC分类号： D06F75/20 , D06F75/30 , D06F75/38
摘要： A steam sprayer is used in ironing textile products, including clothes. The steam sprayer is user-friendly and offers highly effective ironing. A vaporizing chamber generates steam by vaporizing water stored in a tank. A water-supplying unit supplies the vaporizing chamber with the water. A steam-ejecting section spouts the steam outside. The steams-ejecting section includes a ironing surface with high frictional resistance characteristics. When touching the ironing surface to clothes or other fabrics for ironing, a pulling force is caused by the frictional resistance, which is a preferable wrinkles-off effect. Thus, the steam sprayer offers a synergistic effect brought by spouting the steam and applying the pulling force to clothes, thereby neatly fixing the form of clothes.
摘要翻译：蒸汽喷雾器用于熨烫纺织品，包括衣服。蒸汽喷雾器是用户友好的，并提供高效熨烫。蒸发室通过蒸发储存在罐中的水来产生蒸汽。供水单元向蒸发室供应水。蒸汽喷射部分将蒸汽喷出。蒸汽喷射部分包括具有高摩擦阻力特性的熨烫表面。当熨烫表面接触熨烫衣物或其他织物时，由摩擦阻力引起拉力，这是优选的皱纹效果。因此，蒸汽喷雾器通过喷射蒸汽并将拉力施加到衣服而产生协同效应，从而整齐地固定衣服的形式。

35. 发明授权

US6138389A Iron bottom surface with plated metal film 失效
标题翻译：铁底表面镀金属膜
公开(公告)号：US6138389A
公开(公告)日：2000-10-31
申请号：US718940
申请日：1996-09-24
申请人： Narutoshi Kanazawa , Toshimitsu Kurumizawa , Masao Shimizu , Toshihide Nakamura , Shinichiro Kobayashi , Toru Taniguchi , Toshiyuki Maeda , Hiroaki Matsuyoshi , Mitsuaki Yamada , Michio Saito
发明人： Narutoshi Kanazawa , Toshimitsu Kurumizawa , Masao Shimizu , Toshihide Nakamura , Shinichiro Kobayashi , Toru Taniguchi , Toshiyuki Maeda , Hiroaki Matsuyoshi , Mitsuaki Yamada , Michio Saito
IPC分类号： D06F75/38
CPC分类号： D06F75/38
摘要： An iron comprising a bottom member as an ironing face, which is provided with a plated metal film containing fluorine compound fine particles, so that the ironing face provides high surface hardness which shows less wear, good slidability and less generation of static electricity.
摘要翻译：一种熨斗，其包括作为熨烫面的底部构件，其设置有包含氟化合物细颗粒的电镀金属膜，使得熨烫面提供较高的表面硬度，其显示较少的磨损，良好的滑动性和较少的静电产生。

36. 发明授权

US6122849A Iron with thermal resistance layer 有权
标题翻译：铁与热电阻层
公开(公告)号：US6122849A
公开(公告)日：2000-09-26
申请号：US300409
申请日：1999-04-27
申请人： Kenji Kida , Shinichi Nakajima , Toshihide Nakamura , Shinichiro Kobayashi
发明人： Kenji Kida , Shinichi Nakajima , Toshihide Nakamura , Shinichiro Kobayashi
IPC分类号： D06F75/26 , D06F75/38 , D06F75/24
CPC分类号： D06F75/26 , D06F75/38
摘要： An iron has a first base to be directly heated for increasing an amount of accumulated heat, a second base including a soleplate and a thermal resistance layer disposed between the bases. The thermal resistance layer has a greater heat resistance than the first base so that the first base heated by a heater can maintain a higher temperature than the second base. The iron of the present invention can increase an amount of accumulated heat, thereby extending not only an ironing period but also a period for spraying steam. If a weight of the iron is decreased, the iron still can hold approximately the same accumulated heat as a conventional iron so that a lightweight iron can be provided.
摘要翻译：铁具有直接加热以增加积热量的第一基座，包括底板的第二基座和设置在基座之间的热电阻层。热阻层比第一基底具有更高的耐热性，使得由加热器加热的第一基底可以保持比第二基底更高的温度。本发明的铁可以增加累积的热量，从而不仅延长了熨烫时间，而且延长了喷射蒸汽的时间。如果铁的重量减少，则铁仍然能够保持与常规铁大致相同的累积热量，从而可以提供轻质的铁。

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