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    • 38. 发明授权
    • Spectrophotometer
    • 分光光度计
    • US4169678A
    • 1979-10-02
    • US865487
    • 1977-12-29
    • Masaru InoueMakoto IshikawaKenji Fukuda
    • Masaru InoueMakoto IshikawaKenji Fukuda
    • G01J3/02G01J3/42
    • G01J3/42
    • A spectrophotometer used for measurement of the transmittance of a sample according to an optical null method, wherein light from a light source is divided into two light beams and pass through a sample cell and reference cell respectively, chopped by a chopper for alternatively supplying the light beams through a monochrometer to a light detector providing signals which are amplified and subjected to synchronous detection by a synchronous rectifier operating in synchronism with the chopper operation so as to obtain an electric signal proportional to the intensity difference between the light passed through the sample cell and that passed through the reference cell with the intensity of the light passed through the reference cell being controlled by a light attenuator connected to a recording pen until the difference signal becomes null. The spectrophotometer further includes an AC signal source generating an AC signal in synchronous relation with the operation of the chopper for applying the AC signal to the input of the amplifier, a position-voltage transducer providing a signal representing the position of a recording pen, a voltage difference detector detecting the difference between the output voltages of the position-voltage transducer before and after the application of the AC signal, and a voltage variation rate detector detecting rate of transient variation with respect to time of the signal of the position-voltage transducer after application of the AC signal. The optical sensitivity of the spectrophotometer is detected by the voltage difference detector and the electrical gain is detected by the voltage variation rate detector.
    • 用于根据光学零方法测量样品的透射率的分光光度计,其中将来自光源的光分成两束光,并分别通过切片机切碎,以切换供应光 通过单色仪将光束检测器提供给光检测器,提供信号,该信号被同步整流器进行放大和同步检测,同步整流器与斩波器操作同步,以获得与通过样品池的光的强度差成比例的电信号, 通过参考单元,其中通过参考单元的光的强度由连接到记录笔的光衰减器控制,直到差信号变为零。 分光光度计还包括AC信号源,其产生与用于将AC信号施加到放大器的输入端的斩波器的操作同步的AC信号,提供表示记录笔的位置的信号的位置电压换能器, 电压差检测器检测在施加AC信号之前和之后的位置电压传感器的输出电压之间的差异,以及电压变化率检测器检测相对于位置电压传感器的信号的时间的瞬态变化的速率 应用交流信号后。 通过电压差检测器检测分光光度计的光学灵敏度,电压变化率检测器检测电力增益。