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    • 32. 发明授权
    • Device for electrofusion of cells
    • 细胞电凝装置
    • US5007995A
    • 1991-04-16
    • US350715
    • 1989-05-11
    • Yasuo TakahashiKazuo SuzukiToshinobu NiimuraTokio Kano
    • Yasuo TakahashiKazuo SuzukiToshinobu NiimuraTokio Kano
    • C12M3/00C12N13/00
    • C12N13/00C12M35/02
    • A device and a method for fusing biological cells. The device is formed by a chamber having a tube for containing a cell suspension and a cover for sealing the tube. The chamber is provided with a lower electrode having a smooth and flat surface for contacting the cells precipitating in the cell suspension, and an upper electrode opposite to the lower electrode. The lower electrode forms the inner bottom surface of the tube. The upper electrode is inserted in the cover to enter the tube. The chamber is centrifuged to form the layers of cells on the lower electrode at the bottom of the tube. Then, a predetermined ac voltage is applied across the upper and lower electrodes to fuse the cells efficiently. Such a device and method are useful for the production of monoclonal antibodies or giant cells.
    • 用于融合生物细胞的装置和方法。 该装置由具有用于容纳细胞悬浮液的管和用于密封管的盖的室形成。 所述腔室设置有具有光滑平坦表面的下电极,用于使细胞悬浮液中沉淀的细胞接触,以及与下电极相对的上电极。 下电极形成管的内底表面。 上电极插入盖子进入管子。 将腔室离心以在管底部的下电极上形成细胞层。 然后,跨越上下电极施加预定的交流电压以有效地熔化电池。 这种装置和方法可用于生产单克隆抗体或巨细胞。
    • 34. 发明授权
    • Soybean hydrolyzate
    • 大豆水解物
    • US4882180A
    • 1989-11-21
    • US233900
    • 1988-08-18
    • Shoji TakaoHiroshi NakashimaMakoto WatanabeMakoto SaitohKyoko ChibaKazuo Suzuki
    • Shoji TakaoHiroshi NakashimaMakoto WatanabeMakoto SaitohKyoko ChibaKazuo Suzuki
    • A23L11/00A23J3/34
    • A23J3/346
    • A soybean hydrolyzate is disclosed. The hydrolyzate is of a high nutritional value and excellent in protein absorbability. Moreover, it is free from bean-like or grass-like smelling that is characteristic of soybean, as well as from bitter taste. Consequently, the hydrolyzate is very valuable food as protein source.The hydrolyzate is characterized by that the soluble fraction of the hydrolyzate contains amino nitrogen in an amount in the range from 5 to 20%, preferably from 10 to 15% of the total nitrogen of said fraction. The hydrolyzate is prepared by pulverizing soybean, adding water to the pulverized soybean to form a slurry of a solid content in the range from 1 to 20%, heating the slurry at a temperature from 60.degree. to 100.degree. C. for a period from 5 to 180 minutes, homogenizing the slurry under a high pressure and hydrolyzing the homogenates with a neutral protease produced by Bacillus subtilis.
    • 公开了一种大豆水解产物。 水解产物营养价值高,蛋白质吸收性好。 此外,它没有豆类或草样的嗅觉,这是大豆的特征以及苦味。 因此,水解产物是非常有价值的食物作为蛋白质来源。 水解产物的特征在于水解产物的可溶级分含有氨基氮,其量为所述级分总氮的5至20%,优选10至15%。 通过粉碎大豆制备水解产物,向粉碎的大豆中加入水以形成固体含量为1至20%的浆料,在60℃至100℃的温度下加热浆料5分钟 至180分钟,高压均质浆料,并用枯草芽孢杆菌产生的中性蛋白酶水解匀浆物。
    • 37. 发明授权
    • Method for chain-opening polymerization of norbornene derivatives
    • 降冰片烯衍生物的开链聚合方法
    • US4250063A
    • 1981-02-10
    • US5503
    • 1979-01-22
    • Teizo KotaniShuichi MatsumotoKatsutoshi IgarashiKazuo Suzuki
    • Teizo KotaniShuichi MatsumotoKatsutoshi IgarashiKazuo Suzuki
    • C08F4/22C08G61/08C08F4/16C08F18/00
    • C08G61/08
    • Polymers or copolymers of norbornene derivatives can be produced by contacting in the presence or absence of an inert solvent at least one norbornene derivative substituted by at least one polar group selected from the group consisting of ester, ether, nitrile, amide, imide, acid anhydride, halogen, and silyl, or by at least one substituent having one of said polar groups; or a combination of said norbornene derivative with at least one member selected from the group consisting of cycloalkenes having no polar substituent and polymers having olefinic carbon-to-carbon double bonds; with a catalyst consisting essentially of (a) at least one coordination compound of W or Mo, the oxidation number of which is 2, 1 or 0, (b) at least one titanium tetrahalide and, if necessary, (c) at least one compound selected from the group consisting of componds having an electron-accepting .pi. bond, N-halogen-substituted cyclic acid imides, sulfides, sulfoxides and phosphines. The above-mentioned catalyst is more active, cheaper, and easier to handle than the conventional catalysts containing organoaluminum compounds.
    • 降冰片烯衍生物的聚合物或共聚物可以通过在惰性溶剂存在或不存在下使至少一种被至少一个选自酯,醚,腈,酰胺,酰亚胺,酸酐的极性基团取代的降冰片烯衍生物 ,卤素和甲硅烷基,或至少一个具有所述极性基团之一的取代基; 或所述降冰片烯衍生物与选自不具有极性取代基的环烯烃和具有烯烃碳 - 碳双键的聚合物中的至少一种的组合; 催化剂基本上由(a)至少一种W或Mo的配位化合物组成,其氧化数为2,1或0,(b)至少一种四卤化钛,如果需要,(c)至少一种 选自具有电子接受性π键的化合物,N-卤素取代的环状酰亚胺,硫化物,亚砜和膦的化合物。 与含有机铝化合物的常规催化剂相比,上述催化剂更具活性,更便宜且更容易处理。