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    • 34. 发明授权
    • Thermostable xylanases from Microtetraspora flexuosa as a feed additive
    • 作为饲料添加剂来自Microtetraspora flexuosa的热稳定性木聚糖酶
    • US6132716A
    • 2000-10-17
    • US732242
    • 1997-04-07
    • Andrew J. MorganKathleen A. ClarksonElizabeth A. BodieWilliam A. Cuevas
    • Andrew J. MorganKathleen A. ClarksonElizabeth A. BodieWilliam A. Cuevas
    • A23K1/165C12N9/24C12P21/00C12R1/00C12R1/645C12S3/08D21C5/00D21C9/10A61K38/47A23L1/28
    • C12Y302/01032C12N9/248C12Y302/01008D21C5/005Y10S426/807Y10S435/822
    • A feed additive that provides increased feed conversion ratio is prepared containing a physiologically acceptable carrier and one or more of five thermostable xylanases from Microtetraspora flexuosa, preferably strain ATCC 35864. The xylanases can tolerate heating for one minute in a water bath at 95.degree. C. without substantial loss of activity, and tolerate alkaline conditions in a pH range of 7.0-10.0. A feed for poultry or pigs contains the xylanases in a feed containing at least 20% by weight cereal. The five xylanases have the following characteristics: (i) a molecular weight of about 33,100 daltons, a pI of about 8.5, and a maximum activity at pH 7.0-7.5 at a temperature of about 70.degree. C.; (ii) a molecular weight of about 33,300 daltons, a pI of about 7.5, and a maximum activity at pH 7.0-7.5 at a temperature of about 65.degree. C.; (iii) a molecular weight of about 31,000 daltons, a pI of about 6.2, and a maximum activity at pH 7.5 at a temperature of about 65.degree. C.; (iv) a molecular weight of about 50,000 daltons, a pI of about 5.8, and a maximum activity at pH 7.5 at a temperature of about 65.degree. C.; and (v) a molecular weight of about 35,000 daltons, a pI of about 5.3, and a maximum activity at pH 7.5 at a temperature of about 70.degree. C.
    • PCT No.PCT / EP95 / 01628 Sec。 371日期1997年4月7日 102(e)日期1997年4月7日PCT 1995年4月28日PCT PCT。 WO95 / 29997 PCT出版物 日期1995年11月9日制备提供增加的饲料转化率的饲料添加剂,其含有生理学上可接受的载体和来自Microtetraspora flexuosa的五种热稳定性木聚糖酶中的一种或多种,​​优选菌株ATCC 35864.木聚糖酶可以耐受在水浴中加热1分钟 在95℃下没有显着的活性损失,并且在7.0-10.0的pH范围内耐受碱性条件。 家禽或猪的饲料在含有至少20重量%谷物的饲料中含有木聚糖酶。 五种木聚糖酶具有以下特征:(i)约33,100道尔顿的分子量,约8.5的pI值,在约70℃的温度下在pH 7.0-7.5的最大活性。 (ii)约33,300道尔顿的分子量,约7.5的pI,在约65℃的温度下在pH 7.0-7.5的最大活性。 (iii)约31,000道尔顿的分子量,约6.2的pI,在约65℃的温度下在7.5的最大活性。 (iv)约50,000道尔顿的分子量,约5.8的pI,在约65℃的温度下在pH7.5的最大活性。 和(v)约35,000道尔顿的分子量,约5.3的pI,在约70℃的温度下在pH7.5的最大活性。