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    • 34. 发明授权
    • Methods and apparatus for automated base-calling on multiple DNA strands
    • 在多个DNA链上进行自动基本呼叫的方法和装置
    • US08126235B2
    • 2012-02-28
    • US12418933
    • 2009-04-06
    • Xiaomeng ShiMuriel MedardRalf KoetterNuala Ann Koetter, legal representativeDesmond S. Lun
    • Xiaomeng ShiMuriel MedardRalf KoetterDesmond S. Lun
    • G06K9/00
    • G06K9/0053G01N27/44717G01N30/8682G01N30/8696
    • In some aspects, a method of automated base-calling using at least one image obtained from a chemical sequencing process performed simultaneously on a plurality of DNA strands, the at least one image including intensity information corresponding to locations of at least one base in the plurality of DNA strands is provided. The method comprises processing the at least image to obtain a function corresponding to the intensity information in the at least one image for the at least one base, the function incorporating intensity information corresponding to each of the plurality of DNA strands, identifying a plurality of peaks in the function, the plurality of peaks indicating possible locations for the at least one base in the plurality of DNA strands, assigning membership to each of the plurality of peaks by determining whether each of the plurality of peaks is believed to have resulted from none, one or multiple of the plurality of DNA strands, and computing a sequence for the at least one base for each of the plurality of DNA strands based, at least in part, on the membership assignment.
    • 在一些方面,一种使用从多个DNA链同时执行的化学测序过程获得的至少一个图像进行自动基本呼叫的方法,所述至少一个图像包括对应于多个基因中至少一个基因位置的强度信息 的DNA链。 所述方法包括处理所述至少一个图像以获得对应于所述至少一个基底的所述至少一个图像中的强度信息的函数,所述功能包括对应于所述多个DNA链中的每一个的强度信息,识别多个峰 在所述功能中,所述多个峰指示所述多个DNA链中所述至少一个碱基的可能位置,通过确定所述多个峰中的每一个是否被认为是由所述多个峰中的每一个赋予的, 至少部分地基于成员分配来计算多个DNA链中的一个或多个,以及针对所述多个DNA链中的每一个为所述至少一个碱基计算序列。
    • 37. 发明申请
    • METHODS AND APPARATUS FOR AUTOMATED BASE-CALLING ON MULTIPLE DNA STRANDS
    • 用于自动基因多态DNA检测的方法和装置
    • US20100074492A1
    • 2010-03-25
    • US12418933
    • 2009-04-06
    • Xiaomeng ShiMuriel MedardRalf KoetterNuala Ann KoetterDesmond S. Lun
    • Xiaomeng ShiMuriel MedardRalf KoetterNuala Ann KoetterDesmond S. Lun
    • G06K9/00
    • G06K9/0053G01N27/44717G01N30/8682G01N30/8696
    • In some aspects, a method of automated base-calling using at least one image obtained from a chemical sequencing process performed simultaneously on a plurality of DNA strands, the at least one image including intensity information corresponding to locations of at least one base in the plurality of DNA strands is provided. The method comprises processing the at least image to obtain a function corresponding to the intensity information in the at least one image for the at least one base, the function incorporating intensity information corresponding to each of the plurality of DNA strands, identifying a plurality of peaks in the function, the plurality of peaks indicating possible locations for the at least one base in the plurality of DNA strands, assigning membership to each of the plurality of peaks by determining whether each of the plurality of peaks is believed to have resulted from none, one or multiple of the plurality of DNA strands, and computing a sequence for the at least one base for each of the plurality of DNA strands based, at least in part, on the membership assignment.
    • 在一些方面,一种使用从多个DNA链同时执行的化学测序过程获得的至少一个图像进行自动基本呼叫的方法,所述至少一个图像包括对应于多个基因中至少一个基因位置的强度信息 的DNA链。 所述方法包括处理所述至少一个图像以获得对应于所述至少一个基底的所述至少一个图像中的强度信息的函数,所述功能包括对应于所述多个DNA链中的每一个的强度信息,识别多个峰 在所述功能中,所述多个峰指示所述多个DNA链中所述至少一个碱基的可能位置,通过确定所述多个峰中的每一个是否被认为是由所述多个峰中的每一个赋予的, 至少部分地基于成员分配来计算多个DNA链中的一个或多个,以及针对所述多个DNA链中的每一个为所述至少一个碱基计算序列。
    • 40. 发明授权
    • Pseudorandom noise sequence generator
    • 伪随机噪声序列发生器
    • US06201870B1
    • 2001-03-13
    • US09036234
    • 1998-03-06
    • Muriel MedardJohn D. MooresKatherine L. HallKristin A. RauschenbachSalil ParikhAgnes H. Chan
    • Muriel MedardJohn D. MooresKatherine L. HallKristin A. RauschenbachSalil ParikhAgnes H. Chan
    • H04L900
    • H04L9/0668
    • A pseudorandom sequence generator including a first feedback shift register having at least one input and at least one output and a first controller having an output in communication with the at least one input of the first feedback shift register; the first feedback shift register operating at a first speed S1 and the first controller operating at a second speed S2. In one embodiment the first speed S1 of the first feedback shift register is an integer multiple of the second speed S2 of the first controller. In another embodiment the first feedback shift register includes a shift register having an input, an output, and at least one tap; and a feedback function generator having a first input in communication with the at least one tap of the shift register, a second input in communication with the output of the first controller, and an output in communication with the input of the shift register; the feedback function generator includes at least one feedback function.
    • 一种伪随机序列发生器,包括具有至少一个输入和至少一个输出的第一反馈移位寄存器和具有与第一反馈移位寄存器的至少一个输入通信的输出的第一控制器; 第一反馈移位寄存器以第一速度S1操作,第一控制器以第二速度S2操作。 在一个实施例中,第一反馈移位寄存器的第一速度S1是第一控制器的第二速度S2的整数倍。 在另一实施例中,第一反馈移位寄存器包括具有输入,输出和至少一个抽头的移位寄存器; 以及反馈函数发生器,其具有与所述移位寄存器的所述至少一个抽头通信的第一输入,与所述第一控制器的输出通信的第二输入以及与所述移位寄存器的输入通信的输出; 所述反馈函数发生器包括至少一个反馈函数。