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31. 发明授权

US06717035B2 Transgenic plants expressing photorhabdus toxin 有权
标题翻译：转基因植物表达光斑念珠菌毒素
公开(公告)号：US06717035B2
公开(公告)日：2004-04-06
申请号：US10435835
申请日：2003-05-12
申请人： James K. Petell , Donald J. Merlo , Rod A. Herman , Jean L. Roberts , Lining Guo , Barry W. Schafer , Kitisri Sukhapinda , Ann Owens Merlo
发明人： James K. Petell , Donald J. Merlo , Rod A. Herman , Jean L. Roberts , Lining Guo , Barry W. Schafer , Kitisri Sukhapinda , Ann Owens Merlo
IPC分类号： A01H500
CPC分类号： C07K14/24 , C12N15/8286 , Y02A40/162
摘要： Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising the nucleic acid of SEQ ID NO:4 are insect resistant.
摘要翻译：编码昆虫毒素TcdA和TcbA的新型多核苷酸序列具有与天然基因显着不同的基本组成，使得它们与植物基因更相似。新的序列适用于单子叶植物和双子叶植物中的高表达。具有包含SEQ ID NO：4的核酸的基因组的转基因植物具有抗虫性。

32. 发明授权

US06495738B1 Modification of fatty acid composition in plants by expression of a fungal acyl-CoA desaturase 有权
标题翻译：通过真菌酰基辅酶A去饱和酶的表达修饰植物中的脂肪酸组成
公开(公告)号：US06495738B1
公开(公告)日：2002-12-17
申请号：US09280428
申请日：1999-03-29
申请人： Otto Folkerts , Donald J. Merlo
发明人： Otto Folkerts , Donald J. Merlo
IPC分类号： A01H500
CPC分类号： C12N9/0083 , C12N15/8247
摘要： Genes-encoding a delta-9 CoA desaturase from Aspergillus nidulans have been isolated. The proteins encoded by genes, when expressed in a plant, can alter the saturate levels of the oil.
摘要翻译：已经分离了从构巢曲霉编码Δ-9 CoA去饱和酶的基因。由基因编码的蛋白质，当在植物中表达时，可以改变油的饱和水平。

33. 发明授权

US6166302A Modified Bacillus thuringiensis gene for lepidopteran control in plants 失效
标题翻译：改良的苏云金芽孢杆菌基因在植物中的鳞翅目控制
公开(公告)号：US6166302A
公开(公告)日：2000-12-26
申请号：US729601
申请日：1996-10-11
申请人： Donald J. Merlo , Otto Folkerts
发明人： Donald J. Merlo , Otto Folkerts
IPC分类号： C12N15/09 , A01H5/00 , A01H5/10 , C07K14/325 , C12N15/11 , C12N15/32 , C12N15/67 , C12N15/82
CPC分类号： C12N15/8216 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Synthetic DNA sequences which are optimized for expression in plants, particularly maize, and which encode a Bacillus thuringiensis protein that is toxic to specific insects are provided, along with methods for the engineering of any synthetic insecticidal gene in maize.
摘要翻译：提供了优化用于在植物，特别是玉米中表达并且编码对特定昆虫有毒的苏云金芽孢杆菌蛋白质的合成DNA序列，以及在玉米中任何合成杀虫基因工程的方法。

34. 发明申请

US20130067617A1 PLANTS HAVING STRESS RELATED TRAITS AND METHODS FOR MAKING THE SAME 有权
标题翻译：具有应力相关行为的植物及其制备方法
公开(公告)号：US20130067617A1
公开(公告)日：2013-03-14
申请号：US13617207
申请日：2012-09-14
申请人： Avutu S. Reddy , Sun Yuejin , Donald J. Merlo , Weiting W. Ni , Dayakar Pareddy , Zhifang Gao , Gregory A. Bradfisch
发明人： Avutu S. Reddy , Sun Yuejin , Donald J. Merlo , Weiting W. Ni , Dayakar Pareddy , Zhifang Gao , Gregory A. Bradfisch
IPC分类号： A01H5/00 , C12N5/10 , A01H5/10 , C12N15/29 , C12N15/82 , A01H1/02
CPC分类号： C07K14/415 , C12N9/93 , C12N15/8273 , C12Y603/02019
摘要： This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.
摘要翻译：本发明一般涉及通过增加或产生植物中RING蛋白的一种或多种活性而与相应的非转化野生型植物细胞相比，优选在瞬时和重复的非生物胁迫条件下具有增加的产量的植物细胞。

35. 发明申请

US20110203017A1 Novel Herbicide Resistance Genes 有权
标题翻译：新型除草剂抗性基因
公开(公告)号：US20110203017A1
公开(公告)日：2011-08-18
申请号：US12091896
申请日：2006-10-27
申请人： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald J. Merlo , Jayakumar P. Samuel , Gaofeng Lin
发明人： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald J. Merlo , Jayakumar P. Samuel , Gaofeng Lin
IPC分类号： A01H5/00 , C12N5/10 , A01N55/00 , A01N39/04 , C07H21/04 , A01P13/00 , A01H5/10
CPC分类号： C12N15/8274 , C12N9/0071 , C12N15/8275
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
摘要翻译：本发明提供了不仅对2,4-D有抗性的新型植物，而且还提供了对吡啶氧基乙酸酯除草剂的抗性。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

36. 发明申请

US20110124503A1 NOVEL HERBICIDE RESISTANCE GENES 审中-公开
标题翻译：新型抗除草剂基因
公开(公告)号：US20110124503A1
公开(公告)日：2011-05-26
申请号：US12951813
申请日：2010-11-22
申请人： Terry R. Wright , Justin M. Lira , Donald J. Merlo , Nicole L. Arnold
发明人： Terry R. Wright , Justin M. Lira , Donald J. Merlo , Nicole L. Arnold
IPC分类号： A01N57/20 , A01N39/02 , A01H5/00 , A01N47/34 , C12Q1/68 , G01N33/53 , A01P13/00 , A01P13/02
CPC分类号： C12N15/8274 , C12N9/0069 , C12N15/8209
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和改善除草剂抗性管理方案。更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

37. 发明授权

US07902334B2 Pesticidally active proteins and polynucleotides obtainable from Paenibacillus species 有权
标题翻译：杀虫活性蛋白质和可从Paenibacillus物种获得的多核苷酸
公开(公告)号：US07902334B2
公开(公告)日：2011-03-08
申请号：US11775663
申请日：2007-07-10
申请人： Scott B. Bintrim , Scott A. Bevan , Baolong Zhu , Donald J. Merlo
发明人： Scott B. Bintrim , Scott A. Bevan , Baolong Zhu , Donald J. Merlo
IPC分类号： C07K14/00
CPC分类号： C12P19/34 , C07K14/195 , C12P21/02 , C12Q1/689 , C12Q2600/142
摘要： The subject invention provides unique biological alternatives for pest control. More specifically, the present invention relates to novel pesticidal proteins, novel sources of pesticidal proteins, polynucleotides that encode such toxins, and to methods of using these toxins to control insects and other plant pests. The subject invention relates to the surprising discovery that Paenibacillus species, and proteins therefrom, have toxicity to lepidopterans. There have been no known reports of a Paenibacillus species, strain, or protein having toxicity to lepidopterans. This is also the first known example of a Paenibacillus Cry protein that is toxic to lepidopterans. Furthermore, this is the first known report of a Paenibacillus having toxin complex (TC)-like proteins. The DAS1529 isolate disclosed here is also the first known example of a natural bacterium that produces both a Cry toxin and TC proteins. The subject invention also relates to new classes of Cry and TC proteins that are pesticidally active.
摘要翻译：本发明提供了用于病虫害防治的独特的生物学替代物。更具体地，本发明涉及新型杀虫蛋白，杀虫蛋白的新来源，编码这种毒素的多核苷酸，以及使用这些毒素来控制昆虫和其它植物害虫的方法。本发明涉及令人惊奇的发现：Paenibacillus物种及其蛋白质对鳞翅目病毒具有毒性。没有已知的具有对鳞翅目昆虫具有毒性的Paenibacillus物种，菌株或蛋白质的报道。这也是对鳞翅目昆虫具有毒性的第一个已知的Paenibacillus Cry蛋白的例子。此外，这是具有毒素复合物（TC）样蛋白质的Paenibacillus的第一个已知报告。此处公开的DAS1529分离物也是产生Cry毒素和TC蛋白质的天然细菌的第一已知实例。本发明还涉及具有杀虫活性的新类型的Cry和TC蛋白。

38. 发明授权

US07888069B2 Plant-made west nile virus (WNV) vaccines, vectors and plant codon optimized sequences 失效
标题翻译：植物西尼罗病毒（WNV）疫苗，载体和植物密码子优化序列
公开(公告)号：US07888069B2
公开(公告)日：2011-02-15
申请号：US11962924
申请日：2007-12-21
申请人： Kelley A. Smith , Steven R. Webb , Steven L. Evans , Charles A. Mihaliak , Donald J. Merlo , Geoffrey J. Letchworth
发明人： Kelley A. Smith , Steven R. Webb , Steven L. Evans , Charles A. Mihaliak , Donald J. Merlo , Geoffrey J. Letchworth
IPC分类号： C12N15/40 , C12N15/62 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8258 , A61K39/12 , A61K2039/55555 , A61K2039/55566 , C07K14/005 , C12N2770/24122 , C12N2770/24134 , Y02A50/394
摘要： The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.
摘要翻译：本申请提供针对西尼罗河病毒（WNV）多肽及其片段的物质的多种组合物，以及如本文所述编码，指导表达或产生WNV多肽的多核苷酸，载体和转化的宿主细胞。本文还提供了使用多肽和多核苷酸在个体中产生免疫应答或检测WNV特异性或中和抗体的存在的方法。

39. 发明申请

US20100251432A1 Novel Herbicide Resistance Genes 有权
标题翻译：新型除草剂抗性基因
公开(公告)号：US20100251432A1
公开(公告)日：2010-09-30
申请号：US12599385
申请日：2008-05-09
申请人： Justin M. Lira , Erika Megan Snodderley , Andrew E. Robinson , Terry R. Wright , Donald J. Merlo
发明人： Justin M. Lira , Erika Megan Snodderley , Andrew E. Robinson , Terry R. Wright , Donald J. Merlo
IPC分类号： A01H5/00 , C12N5/10 , A01N57/00 , A01N39/02 , C07H21/04
CPC分类号： C12N15/8274 , C12N9/0004 , C12N15/8275
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
摘要翻译：本发明提供了不仅对2,4-D有抗性的新型植物，而且还涉及吡啶氧基乙酸酯除草剂。本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。根据本发明使用的优选酶和基因在本文中称为AAD-13（丙烯酰氧基烷酸酯加氧酶）。这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

40. 发明授权

US06590142B1 Transgenic plants expressing photorhabdus toxin 有权
公开(公告)号：US06590142B1
公开(公告)日：2003-07-08
申请号：US09637048
申请日：2000-08-11
申请人： James K. Petell , Donald J. Merlo , Rod A. Herman , Jean L. Roberts , Lining Guo , Barry W. Schafer , Kitisri Sukhapinda , Ann Owens Merlo
发明人： James K. Petell , Donald J. Merlo , Rod A. Herman , Jean L. Roberts , Lining Guo , Barry W. Schafer , Kitisri Sukhapinda , Ann Owens Merlo
IPC分类号： A01H500
CPC分类号： C07K14/24 , C12N15/8286 , Y02A40/162
摘要： Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising a nucleic acid of SEQ ID NO: 3 or SEQ ID NO:4 are insect resistant.

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