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31. 发明申请

US20110189742A1 METHOD FOR THE PRODUCTION OF FREE CARBOXYLIC ACIDS 失效
标题翻译：生产游离羧酸的方法
公开(公告)号：US20110189742A1
公开(公告)日：2011-08-04
申请号：US13002519
申请日：2009-04-29
申请人： Thomas Haas , Thomas Tacke , Achim Marx , Alexander Schraven , Olivier Zehnacker , Eva Maria Wittmann
发明人： Thomas Haas , Thomas Tacke , Achim Marx , Alexander Schraven , Olivier Zehnacker , Eva Maria Wittmann
IPC分类号： C12P7/40
CPC分类号： C12P7/40 , C07C51/02 , C07C51/41 , C07C51/47 , C12P7/42 , C07C59/01 , C07C59/06 , C07C59/255 , C07C59/08 , C07C59/265
摘要： A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R1, R2 and R3, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.
摘要翻译：一种制备游离羧酸的方法，包括：A）通过加入式（I）的胺的位于水性介质中的生物细胞制备羧酸，其中R1，R2和R3彼此独立地相同或不同的支链或非支链的任选取代的烃基或H; B）对于添加的胺A）是水溶性的，加入式（I）的水不溶性胺，其中在A）或B）中获得多相体系，并且相应的羧酸铵由水不溶性胺和羧酸; C）去除水不溶性相; 和D）释放游离羧酸时加热水不溶性相。

32. 发明申请

US20100068773A1 MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译： 3-羟基丁酸的微生物生产
公开(公告)号：US20100068773A1
公开(公告)日：2010-03-18
申请号：US12303161
申请日：2007-06-01
申请人： Achim Marx , Markus Poetter , Stefen Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefen Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
IPC分类号： C12P7/62 , C12N1/21 , C12P7/42 , C07H21/04 , C12N15/74 , C07K14/00
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.
摘要翻译：本发明涉及与其野生型相比被修饰的细胞，使得其能与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸相比形成更多的细胞甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。

33. 发明授权

US10174349B2 Recombinant cell producing 2-hydroxyisobutyric acid 有权
公开(公告)号：US10174349B2
公开(公告)日：2019-01-08
申请号：US13001204
申请日：2009-04-28
申请人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
发明人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
IPC分类号： C12P7/42 , C12P7/62
摘要： The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.

34. 发明授权

US08980594B2 Use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase 有权
标题翻译：使用与MeaB蛋白质同源的蛋白质来增加3-羟基羧酸-CoA变位酶的酶活性
公开(公告)号：US08980594B2
公开(公告)日：2015-03-17
申请号：US13500041
申请日：2010-10-11
申请人： Liv Reinecke , Steffen Schaffer , Tim Koehler , Anja Thiessenhusen , Achim Marx , Markus Buchhaupt
发明人： Liv Reinecke , Steffen Schaffer , Tim Koehler , Anja Thiessenhusen , Achim Marx , Markus Buchhaupt
IPC分类号： C12P7/42 , C12P7/40 , C12P7/62 , C12N9/90 , C12N9/14 , C12P7/52 , C07K14/195
CPC分类号： C12P7/52 , C07K14/195 , C07K2319/00 , C12N9/90 , C12P7/42
摘要： The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
摘要翻译：本发明涉及使用与MeaB蛋白质同源的蛋白质来增加3-羟基羧酸-CoA变位酶的酶活性，包含3-羟基羧酸-CoA变位酶的融合蛋白和与MeaB蛋白质同源的蛋白质序列以及用于生产2-羟基异丁酸的酶法。

35. 发明申请

US20110171702A1 RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译：重组细胞生产2-羟基丁酸
公开(公告)号：US20110171702A1
公开(公告)日：2011-07-14
申请号：US13001204
申请日：2009-04-28
申请人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
发明人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
IPC分类号： C12P7/40 , C12N5/10 , C12N1/00 , C12P7/42 , C12P7/62
CPC分类号： C12P7/625 , C12P7/42
摘要： The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.
摘要翻译：本发明涉及一种经遗传修饰以能够产生比其野生型更多的2-羟基异丁酸或更多含2-羟基异丁酸单体单元的聚羟基链烷酸酯的细胞，其特征在于2-羟基异丁酸或含有2-羟基异丁酸的聚羟基链烷酸酯，通过作为中间体的乙酰乙酰辅酶A和作为前体的3-羟基丁酰辅酶A制备羟基异丁酸单体单元。

36. 发明申请

US20100190224A1 ENZYME FOR THE PRODUCTION OF METHYLMALONYL-COENZYME A OR ETHYLMALONYL-COENZYME A AND USE THEREOF 审中-公开
标题翻译：用于生产甲基亚甲基二甲基丙烯酸酯或其乙烯基 - 苯甲醚的酶及其用途
公开(公告)号：US20100190224A1
公开(公告)日：2010-07-29
申请号：US12593090
申请日：2008-03-27
申请人： Markus Poetter , Achim Marx , Georg Fuchs , Birgit Alber , Tobias Juergen Erb
发明人： Markus Poetter , Achim Marx , Georg Fuchs , Birgit Alber , Tobias Juergen Erb
IPC分类号： C12P7/42 , C12N15/74 , C12N9/00 , C12N1/21 , C12N15/63 , C07H21/04
CPC分类号： C12P7/52 , C12N9/506 , C12P7/42
摘要： The present invention relates to an isolated DNA, which is selected from the following sequences: a) a sequence according to SEQ ID No. 01, b) an intron-free sequence that is derived from a sequence according to a) and encodes the same protein or peptide as the sequence according to SEQ ID No. 01, c) a sequence that encodes a protein or peptide, which comprises the amino acid sequence according to SEQ ID No. 02, d) a sequence that is at least 80% identical to a sequence according to a) to c), e) a sequence that hybridizes with the antisense strand of a sequence according to one of the groups a) to d) or would hybridize taking into account degeneration of the genetic code, f) a derivative of a sequence according to one of the groups a) to e) obtained by substitution, addition, inversion and/or deletion of one or more bases, g) a sequence that corresponds to SEQ ID No. 01 within the degeneration of the genetic code, h) a sequence with neutral sense mutations of SEQ ID No. 01, and i) a sequence complementary to a sequence according to one of the groups a) to h). The invention further relates to a vector, the use of this vector for transformation of a cell, a transformed cell, a polypeptide, cells genetically engineered relative to their wild type, a method of production of a genetically engineered cell, the genetically engineered cell obtainable by this method, the use of this cell and a method of production of 3-hydroxyisobutyric acid or of a derivative thereof.
摘要翻译：本发明涉及分离的DNA，其选自以下序列：a）根据SEQ ID No 01的序列，b）源自根据a）的序列的内含子序列，并编码它们蛋白质或肽，其为编码蛋白质或肽的序列，其包含根据SEQ ID No.22的氨基酸序列，d）至少80％相同的序列根据a）至c）的序列，e）与根据a）至d）中的一个组的序列的反义链杂交的序列或将考虑到遗传密码的变性而杂交，f）a 根据通过一个或多个碱基的取代，添加，倒位和/或缺失得到的a）至e）中的一个序列的序列的衍生物，g）在遗传变性中对应于SEQ ID No 01的序列代码，h）具有SEQ ID No 01的中性有义突变的序列，和i）与根据a）至h）中的一个组合的序列互补的序列。本发明进一步涉及载体，该载体用于转化细胞，转化细胞，多肽，相对于其野生型基因工程改造的细胞，遗传工程细胞的生产方法，可获得的遗传工程改造的细胞通过该方法，可以使用该细胞和3-羟基异丁酸或其衍生物的制造方法。

37. 发明申请

US20100291644A1 PROCESS FOR PREPARING METHACRYLIC ACID OR METHACRYLIC ESTERS 有权
标题翻译：制备甲基丙烯酸或甲基丙烯酸酯的方法
公开(公告)号：US20100291644A1
公开(公告)日：2010-11-18
申请号：US12602593
申请日：2008-05-30
申请人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
IPC分类号： C12P7/62 , C12P7/40 , C12P7/44
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.
摘要翻译：本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸，如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸酸，并且在适当的情况下酯化甲基丙烯酸。本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

38. 发明授权

US06569650B1 Process for the fermentative preparation of metabolic products and for the nucleotide sequences encoding for the sod gene 失效
标题翻译：用于代谢产物的发酵制备和编码sod基因的核苷酸序列的方法
公开(公告)号：US06569650B1
公开(公告)日：2003-05-27
申请号：US09373731
申请日：1999-08-13
申请人： Muriel Merkamm , Armel Guyonvarch , Achim Marx
发明人： Muriel Merkamm , Armel Guyonvarch , Achim Marx
IPC分类号： C12P1304
CPC分类号： C12N9/0089 , C12P13/08 , C12R1/15
摘要： The present invention is directed to nucleotide sequences coding for the superoxide dismutase (sod) gene from Corynebacterium melassecola. It includes processes for the fermentative preparation of nucleotides, vitamins and L-amino acids using coryneform bacteria in which the sod gene is amplified.
摘要翻译：本发明涉及编码来自黑曲霉（Corynebacterium melassecola）的超氧化物歧化酶（sod）基因的核苷酸序列。它包括使用其中sod基因被扩增的棒状细菌发酵制备核苷酸，维生素和L-氨基酸的方法。

39. 发明授权

US08703451B2 Method for the production of free carboxylic acids 失效
标题翻译：游离羧酸生产方法
公开(公告)号：US08703451B2
公开(公告)日：2014-04-22
申请号：US13002519
申请日：2009-04-29
申请人： Thomas Haas , Thomas Tacke , Achim Marx , Alexander Schraven , Olivier Zehnacker , Eva Maria Wittmann
发明人： Thomas Haas , Thomas Tacke , Achim Marx , Alexander Schraven , Olivier Zehnacker , Eva Maria Wittmann
IPC分类号： C12P7/40 , C07C51/47 , C07C51/41 , C12P7/42
CPC分类号： C12P7/40 , C07C51/02 , C07C51/41 , C07C51/47 , C12P7/42 , C07C59/01 , C07C59/06 , C07C59/255 , C07C59/08 , C07C59/265
摘要： A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R1, R2 and R3, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.
摘要翻译：一种制备游离羧酸的方法，包括：A）通过加入式（I）的胺的位于水性介质中的生物细胞制备羧酸，其中R1，R2和R3彼此独立地相同或不同的支链或非支链的任选取代的烃基或H; B）对于添加的胺A）是水溶性的，加入式（I）的水不溶性胺，其中在A）或B）中获得多相体系，并且相应的羧酸铵由水不溶性胺和羧酸; C）去除水不溶性相; 和D）释放游离羧酸时加热水不溶性相。

40. 发明申请

US20120264182A1 USE OF A PROTEIN HOMOLOGOUS TO A MEAB PROTEIN FOR INCREASING THE ENZYMATIC ACTIVITY OF A 3-HYDROXYCARBOXYLIC ACID-COA MUTASE 有权
标题翻译：蛋白质同源异构体对美国蛋白质的使用，用于增加3-羟基羧基酸 - 椰子油酸酯酶的酶活性
公开(公告)号：US20120264182A1
公开(公告)日：2012-10-18
申请号：US13500041
申请日：2010-10-11
申请人： Liv Reinecke , Steffen Schaffer , Tim Koehler , Anja Thiessenhusen , Achim Marx , Markus Buchhaupt
发明人： Liv Reinecke , Steffen Schaffer , Tim Koehler , Anja Thiessenhusen , Achim Marx , Markus Buchhaupt
IPC分类号： C12N9/96 , C12P7/62 , C12P7/40 , C07H21/04 , C12P7/42
CPC分类号： C12P7/52 , C07K14/195 , C07K2319/00 , C12N9/90 , C12P7/42
摘要： The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
摘要翻译：本发明涉及使用与MeaB蛋白质同源的蛋白质来增加3-羟基羧酸-CoA变位酶的酶活性，包含3-羟基羧酸-CoA变位酶的融合蛋白和与MeaB蛋白质同源的蛋白质序列以及用于生产2-羟基异丁酸的酶法。

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