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31. 发明申请

US20080027213A1 Chimeric immunogens 失效
公开(公告)号：US20080027213A1
公开(公告)日：2008-01-31
申请号：US11808807
申请日：2007-06-13
申请人： Michel Klein , Run-Pan Du , Mary Ewasyshyn
发明人： Michel Klein , Run-Pan Du , Mary Ewasyshyn
IPC分类号： C07K14/005
CPC分类号： G01N33/56983 , A61K39/00 , C07K14/005 , C07K2319/00 , C07K2319/02 , C07K2319/40 , C12N2760/18522 , C12N2760/18622 , G01N2333/115 , G01N2333/135
摘要： Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

32. 发明申请

US20060159709A1 Recombinant high molecular weight major outer membrane protein of moraxella 审中-公开
标题翻译：重组高分子量主要外膜蛋白的moraxella
公开(公告)号：US20060159709A1
公开(公告)日：2006-07-20
申请号：US11013711
申请日：2004-12-16
申请人： Sheena Loosmore , Ken Sasaki , Yan Yang , Michel Klein
发明人： Sheena Loosmore , Ken Sasaki , Yan Yang , Michel Klein
IPC分类号： A61K39/02 , C07H21/04 , C12P21/06 , C12N15/74 , C12N1/21 , C07K14/195
CPC分类号： C07K14/212
摘要： An isolated and purified outer membrane protein of a Moraxella strain, particularly M. catarrhalis, having a molecular mass of about 200 kDa, is provided by recombinant means. The about 200 kDa outer membrane protein as well as nucleic acid molecules encoding the same are useful in diagnostic applications and immunogenic compositions, particularly for in vivo administration to a host to confer protection against disease caused by a bacterial pathogen that produces the about 200 kDa outer membrane protein or produces a protein capable of inducing antibodies in a host specifically reactive with the about 200 kDa outer membrane protein.
摘要翻译：通过重组方法提供分离和纯化的分子量为约200kDa的莫拉氏菌属菌株，特别是卡他莫拉氏菌的外膜蛋白。大约200kDa的外膜蛋白以及编码它们的核酸分子可用于诊断应用和免疫原性组合物，特别是用于体内施用于宿主以赋予产生约200kDa外部细菌病原体引起的疾病的保护产生能够在与约200kDa外膜蛋白特异性反应的宿主中诱导抗体的蛋白质。

33. 发明授权

US07018637B2 Multi-oligosaccharide glycoconjugate bacterial meningitis vaccines 失效
标题翻译：多糖糖缀合细菌性脑膜炎疫苗
公开(公告)号：US07018637B2
公开(公告)日：2006-03-28
申请号：US09027956
申请日：1998-02-23
申请人： Pele Chong , Alf Lindberg , Michel Klein
发明人： Pele Chong , Alf Lindberg , Michel Klein
IPC分类号： A61K39/385 , A61K39/00 , A61K39/095 , C07K1/00 , C07K14/00
CPC分类号： G01N33/56944 , A61K39/092 , A61K39/095 , A61K39/385 , A61K2039/6037 , A61K2039/645 , Y02A50/466 , Y02A50/484 , Y02A50/59 , Y10S530/807 , Y10S530/81
摘要： Multivalent immunogenic molecules comprise a carrier molecule containing at least one functional T-cell epitope and multiple different carbohydrate fragments each linker to the carrier molecule and each containing at least one functional B-cell epitope. The carrier molecule inputs enhanced immunogenicity to the multiple carbohydrate fragments. The carbohydrate fragments may be capsular oligosaccharide fragments from Streptococcus pneumoniae, which may be serotypes 1, 4, 5, 6B, 9V, 14, 18C, 19F or 23F, or Neisseria meningitidis, which may be serotype A, B, C, W-135 or Y. Such oligosaccharide fragments may be sized from 2 to 5 kDa. Alternatively, the carbohydrate fragments may be fragments of carbohydrate-based tumor antigens, such as Globo H, LeY or STn. The multivalent molecules may be produced by random conjugation or site-directed conjugation of the carbohydrate fragments to the carrier molecule. The multivalent molecules may be employed in vaccines or in the generation of antibodies for diagnostic application.
摘要翻译：多价免疫原性分子包含含有至少一个功能性T细胞表位和多个不同碳水化合物片段的载体分子，每个接头与载体分子并且每个包含至少一个功能性B细胞表位。载体分子对多个碳水化合物片段的免疫原性增强。碳水化合物片段可以是来自肺炎链球菌的荚膜寡糖片段，其可以是血清型1,4,5,6B，9V，14,18C，19F或23F或脑膜炎奈瑟氏球菌，其可以是血清型A，B，C，W- 135或Y.这样的寡糖片段的大小可以为2至5kDa。或者，碳水化合物片段可以是基于碳水化合物的肿瘤抗原的片段，例如Globo H，Le Y Y或ST n。多价分子可以通过碳水化合物片段与载体分子的随机缀合或位点定向缀合产生。多价分子可以用于疫苗或产生用于诊断应用的抗体。

34. 发明申请

US20050089525A1 Subunit respiratory syncytial virus vaccine preparation 审中-公开
标题翻译：亚单位呼吸道合胞病毒疫苗的制备
公开(公告)号：US20050089525A1
公开(公告)日：2005-04-28
申请号：US10488241
申请日：2002-09-03
申请人： George Cates , Michel Klein , Ray Ooman , Sonia Sanhueza
发明人： George Cates , Michel Klein , Ray Ooman , Sonia Sanhueza
IPC分类号： A61K39/00 , A61K39/155 , C07K14/135 , C12N7/00 , C12N7/02 , C12N7/04 , G01N33/569 , A61K39/12
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/155 , A61K2039/55505 , A61K2039/55511 , A61K2039/55577 , A61K2039/70 , C07K2319/00 , C12N7/00 , C12N2760/18521 , C12N2760/18522 , C12N2760/18534 , C12N2760/18551 , C12N2760/18561 , G01N33/56983
摘要： The fusion (F) protein, attachment (G) protein and matrix (M) protein of respiratory syncytial virus (RSV) are isolated and purified from respiratory syncytial virus by mild detergent extraction of the proteins from concentrated virus, loading the protein onto a hydroxyapatite or other ion-exchange matrix column and eluting the protein using mild salt treatment. The F, G and M proteins, formulated as immunogenic compositions, are safe and highly immunogenic and protect relevant animal models against decreased caused by respiratory syncytial virus infection.
摘要翻译：呼吸道合胞病毒（RSV）的融合（F）蛋白，附着（G）蛋白和基质（M））蛋白从呼吸道合胞病毒中分离并纯化，通过温和洗涤从浓缩病毒中提取蛋白质，将蛋白质加载到羟基磷灰石上或其他离子交换基质柱，并使用温和盐处理洗脱该蛋白质。配制为免疫原性组合物的F，G和M蛋白是安全和高度免疫原性的，并保护相关动物模型免受呼吸道合胞病毒感染引起的降低。

35. 发明授权

US06368589B1 Use of autologous promoters to express gene products in Bordetella 失效
标题翻译：使用自体启动子在博德特氏菌中表达基因产物
公开(公告)号：US06368589B1
公开(公告)日：2002-04-09
申请号：US08460565
申请日：1995-06-02
申请人： Sheena Loosmore , Gavin Zealey , Reza Khayyam Yacoob , Michel Klein
发明人： Sheena Loosmore , Gavin Zealey , Reza Khayyam Yacoob , Michel Klein
IPC分类号： A01N6300
CPC分类号： C07K14/235 , A61K39/00 , A61K39/099 , C12N15/74
摘要： Autologous promoters are used to effect expression of gene products in Bordetella strains. Hybrid Bordetella genes are constructed comprising a Bordetella gene, particularly one encoding a Bordetella antigen, fused at an ATG codon to a native but autologous Bordetella promoter or other Bordetella strain. Genes and promoters from B. pertussis are preferred. B. pertussis, containing the hybrid gene by insertion into the chromosome of the organism by homologous recombination at specific loci, effects expression of the protein for which the Bordetella gene codes at a production rate different from that achieved for the homologous gene. Specific strains and plasmids are described.
摘要翻译：自体启动子用于在博德氏菌菌株中表达基因产物。构建了混合的博德特氏病基因，其包含与ATG密码子融合到天然但自体博德特氏菌启动子或其他博德氏菌属菌株的博德特氏菌基因，特别是一种编码伯氏杆菌抗原的基因。百日咳酵母的基因和启动子是优选的。百日咳杆菌通过在特定基因座处同源重组插入到生物体的染色体中，包含杂交基因，以不同于同源基因的生产速率影响博德特氏菌基因编码的蛋白质的表达。描述了特异性菌株和质粒。

36. 发明授权

US06013514A Haemophilus outer membrane protein 失效
标题翻译：嗜血杆菌外膜蛋白
公开(公告)号：US06013514A
公开(公告)日：2000-01-11
申请号：US433522
申请日：1995-09-12
申请人： Pele Chong , Wayne Thomas , Yan Ping Yang , Sheena Loosmore , Dwo Yuan Charles Sia , Michel Klein
发明人： Pele Chong , Wayne Thomas , Yan Ping Yang , Sheena Loosmore , Dwo Yuan Charles Sia , Michel Klein
IPC分类号： G01N33/569 , A61K39/00 , A61K39/102 , A61K39/12 , A61K39/395 , A61P31/04 , C07K14/11 , C07K14/195 , C07K14/285 , C07K16/12 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/19 , C07H21/04
CPC分类号： C07K14/285 , C07K16/1242 , A61K39/00 , C07K2317/34
摘要： Purified and isolated nucleic acid from specific strains of Haemophilus influenzae is provided which encodes at least a portion of the D15 outer membrane protein of Haemophilus. The nucleic acid is used to produce peptides, polypeptides and proteins free of contaminant associated with Haemophilus for purposes of diagnosis and medical treatment. Furthermore, the nucleic acid may be used in the diagnosis of Haemophilus infection. Antisera obtained following immunization with the nucleic acid D15 outer membrane protein or peptides also may be used for the purpose of diagnosis and medical treatment.
摘要翻译： PCT No.PCT / CA93 / 00501 Sec。 371 1995年9月12日第 102（e）日期1995年9月12日PCT 1993年11月23日PCT公布。公开号WO94 / 12641 日期1994年6月9日提供了来自嗜血杆菌流感嗜血杆菌的特异菌株的纯化和分离的核酸，其编码嗜血杆菌的D15外膜蛋白的至少一部分。为了诊断和治疗的目的，该核酸用于产生不含与嗜血杆菌相关的污染物的肽，多肽和蛋白质。此外，核酸可用于诊断嗜血杆菌感染。用核酸D15外膜蛋白或肽免疫后获得的抗血清也可用于诊断和治疗的目的。

37. 发明授权

US5817318A Synthetic peptides for an HIV-1 vaccine 失效
标题翻译： HIV-1疫苗的合成肽
公开(公告)号：US5817318A
公开(公告)日：1998-10-06
申请号：US467972
申请日：1995-06-06
申请人： Dwo Yuan Charles Sia , Pele Chong , Michel Klein
发明人： Dwo Yuan Charles Sia , Pele Chong , Michel Klein
IPC分类号： A61K39/00 , C07K14/16 , A61K39/21 , A61K39/38 , C07K1/00
CPC分类号： C07K14/005 , A61K39/00 , C12N2740/16222
摘要： The invention discloses the identification, characterization and use of a synthetic peptide, GPKEPFRDYVDRFYK (p24E), as a T-cell carrier for the appropriate assembly, with defined B-cell epitopes of the HIV-1 envelope protein, for the construction of potential synthetic vaccine candidates against HIV.
摘要翻译：本发明公开了鉴定，表征和使用合成肽GPKEPFRDYVDRFYK（p24E）作为用于适当组装的T细胞载体，其具有HIV-1包膜蛋白的定义的B细胞表位，用于构建潜在的合成针对艾滋病毒的疫苗候选人。

38. 发明授权

US5708149A Method for producing purified recombinant Haemophilus influenzae transferrin binding proteins 失效
标题翻译：生产纯化的重组流感嗜血杆菌转铁蛋白结合蛋白的方法
公开(公告)号：US5708149A
公开(公告)日：1998-01-13
申请号：US487890
申请日：1995-06-07
申请人： Sheena Loosmore , Robin Harkness , Anthony Schryvers , Pele Chong , Scott Gray-Owen , Yan-Ping Yang , Andrew Murdin , Michel Klein
发明人： Sheena Loosmore , Robin Harkness , Anthony Schryvers , Pele Chong , Scott Gray-Owen , Yan-Ping Yang , Andrew Murdin , Michel Klein
IPC分类号： G01N33/569 , A61K9/127 , A61K9/48 , A61K9/50 , A61K35/76 , A61K38/00 , A61K39/00 , A61K39/102 , A61K39/395 , A61K48/00 , A61P31/04 , A61P37/04 , C07H21/04 , C07K14/285 , C07K14/705 , C07K16/12 , C07K16/28 , C12N1/21 , C12N15/09 , C12N15/12 , C12P21/02 , C12R1/19 , C12R1/21
CPC分类号： C07K14/285 , C07K16/2881 , A61K2039/51 , A61K39/00 , C07K2317/34 , C12N2799/021
摘要： Purified and isolated nucleic acid is provided which encodes a transferrin receptor protein of a strain of Haemophilus or a fragment or an analog of the transferrin receptor protein. The nucleic acid sequence may be used to produce peptides free of contaminants derived from bacteria normally containing the Tbp1 or Tbp2 proteins for purposes of diagnostics and medical treatment. Furthermore, the nucleic acid molecule may be used in the diagnosis of infection. Also provided are recombinant Tbp1 or Tbp2 and methods for purification of the same. Live vectors expressing epitopes of transferrin receptor protein for vaccination are provided.
摘要翻译：提供了纯化和分离的核酸，其编码嗜血杆菌菌株的转铁蛋白受体蛋白或转铁蛋白受体蛋白的片段或类似物。为了诊断和治疗目的，核酸序列可用于产生不含通常含有Tbp1或Tbp2蛋白的细菌的不含污染物的肽。此外，核酸分子可以用于感染的诊断。还提供了重组Tbp1或Tbp2及其纯化方法。提供了表达转铁蛋白受体蛋白表位的活载体用于疫苗接种。

39. 发明授权

US5667787A Purification of a pertussis outer membrane protein 失效
标题翻译：百日咳外膜蛋白的纯化
公开(公告)号：US5667787A
公开(公告)日：1997-09-16
申请号：US433644
申请日：1995-05-04
申请人： Gail Jackson , Raafat Fahim , Larry Tan , Pele Chong , John Vose , Michel Klein
发明人： Gail Jackson , Raafat Fahim , Larry Tan , Pele Chong , John Vose , Michel Klein
IPC分类号： A61K39/00 , A61K39/10 , A61P31/04 , C07K1/18 , C07K1/34 , C07K14/005 , C07K14/195 , C07K14/235 , C07K14/41 , C12P21/00 , C12R1/01 , A23J1/00 , C07K1/00 , C08H1/00
CPC分类号： C07K14/235 , A61K39/00 , Y10S530/825
摘要： Pertactin (formerly 69 kDa protein) is recovered in stable biologically pure form having no detectable adenylate cyclase activity from fermentation broth from the fermentation of Bordetella pertussis as well as from the cells. The broth is processed to selectively remove pertussis toxin (PT) and filamentous haemagglutinin (FHA), the pertactin is precipitated by ammonium sulphate and the precipitate is dissolved in buffer at pH 6.0 to 8.5, the solution then is passed through hydroxyapatite and ion-exchange chromatograph columns before final ultrafiltration. Cells are extracted with urea and the extract ultrafiltered and diafiltered. The pertactin is precipitated from the extract and the precipitate processed as above. In a variation, the broth is contacted with ammonium sulphate to precipitate pertactin, PT and FHA, the precipitate is dissolved and the PT and FHA selectively removed, before the solution is passed to the chromatograph columns.
摘要翻译：从稳定的生物纯的形式中回收Pertactin（以前的69kDa的蛋白质），其发酵培养液中不含可检测的腺苷酸环化酶活性，来自百日咳博德特氏菌以及细胞的发酵。处理肉汤以选择性地除去百日咳毒素（PT）和丝状血凝素（FHA），通过硫酸铵沉淀百日咳素，将沉淀物溶于pH 6.0-8.5的缓冲液中，然后将溶液通过羟基磷灰石和离子交换最后超滤前的色谱柱。用尿素萃取细胞，超滤和渗滤提取物。从提取物中沉淀出促黄体生成素，并如上所述处理沉淀物。在一个变化中，将肉汤与硫酸铵接触以沉淀pertactin，PT和FHA，沉淀物溶解，并且在溶液通过色谱柱之前选择性地除去PT和FHA。

40. 发明授权

US5643753A Use of autologous promoters to express gene products in bordetella 失效
标题翻译：使用自体启动子在博来氏菌中表达基因产物
公开(公告)号：US5643753A
公开(公告)日：1997-07-01
申请号：US004301
申请日：1993-01-14
申请人： Sheena Loosmore , Gavin Zealey , Reza Khayyam Yacoob , Michel Klein
发明人： Sheena Loosmore , Gavin Zealey , Reza Khayyam Yacoob , Michel Klein
IPC分类号： A61K39/00 , A61K39/10 , C07K14/235 , C12N1/21 , C12N15/74 , C12N15/64 , C12N15/31
CPC分类号： C07K14/235 , A61K39/099 , C12N15/74 , A61K39/00
摘要： Autologous promoters are used to effect expression of gene products in Bordetella strains. Hybrid Bordetella genes are constructed comprising a Bordetella gene, particularly one encoding a Bordetella antigen, fused at an ATG codon to a native but autologous Bordetella promoter or other Bordetella strain. Genes and promoters from B. pertussis are preferred. B. pertussis, containing the hybrid gene by insertion into the chromosome of the organism by homologous recombination at specific loci, effects expression of the protein for which the Bordetella gene codes at a production rate different from that achieved for the homologous gene. Specific strains and plasmids are described.
摘要翻译：自体启动子用于在博德氏菌菌株中表达基因产物。构建了混合的博德特氏病基因，其包含与ATG密码子融合到天然但自体博德特氏菌启动子或其他博德氏菌属菌株的博德特氏菌基因，特别是一种编码伯氏杆菌抗原的基因。百日咳酵母的基因和启动子是优选的。百日咳杆菌通过在特定基因座处同源重组插入到生物体的染色体中，包含杂交基因，以不同于同源基因的生产速率影响博德特氏菌基因编码的蛋白质的表达。描述了特异性菌株和质粒。

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