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    • 38. 发明申请
    • METHODS AND COMPOSITIONS FOR THE MODULATION OF AMINO ACID BIOSYNTHESIS
    • 用于调节氨基酸生物合成的方法和组合物
    • US20100115661A1
    • 2010-05-06
    • US12581331
    • 2009-10-19
    • John H. DuesingAnthony J. KinneyDaniel Siehl
    • John H. DuesingAnthony J. KinneyDaniel Siehl
    • C12N15/82A01H5/00
    • C12N15/8243C12N15/8251C12N15/8253C12N15/8254
    • Compositions and methods for increasing the level of one or more selected free ε and/or α-N-acetylated amino acids in a selected tissue or organ of a plant are provided. In specific embodiments, the plant, plant part, seed or grain comprises a free α-N-acetylated amino acid content of at least 200 ppm or a ratio of free α-N-acetylated amino acid content to free non-α-N-acetylated amino acid content of about 3 to about 1000. Compositions comprising plants, plant parts, seed and grain having stably incorporated into their genome a heterologous polynucleotide encoding an amino acid-N-acetyltransferase polypeptide operably linked to a promoter active in the seed are provided. Further provided are compositions comprising a plant, plant part, seed or grain having stably incorporated into their genome a first heterologous polynucleotide encoding a first amino acid-N-acetyltransferase polypeptide operably linked to a first promoter active in the seed and a second heterologous polynucleotide encoding a second amino acid-N-acetyltransferase polypeptide operably linked to a second promoter active in the seed, wherein the first and the second amino acid-N-acetyltransferase polypeptide acetylate the α-amine of distinct amino acids. Compositions comprising food sources, feed and supplements, along with methods of increasing the nutritional value of a plant, plant part, seed or grain, are further provided.
    • 用于增加一个或多个所选择的免费等级的组合和方法 和/或α-N-乙酰化氨基酸在植物的选定组织或器官中。 在具体实施方案中,植物,植物部分,种子或颗粒包含至少200ppm的游离α-N-乙酰化氨基酸含量或游离的α-N-乙酰化氨基酸含量与游离的非α-N- 提供约3至约1000的乙酰化氨基酸含量。提供包含植物,植物部分,种子和颗粒的组合物,其稳定地并入其基因组中,编码与种子中活性物质活化的启动子可操作地连接的氨基酸-N-乙酰转移酶多肽的异源多核苷酸 。 进一步提供的是包含植物,植物部分,种子或谷物的组合物,其稳定地并入其基因组中,第一异源多核苷酸编码与种子中有活性的第一启动子可操作地连接的第一氨基酸-N-乙酰转移酶多肽和第二异源多核苷酸编码 可操作地连接到在种子中有活性的第二启动子的第二氨基酸-N-乙酰转移酶多肽,其中所述第一和第二氨基酸-N-乙酰转移酶多肽对不同氨基酸的α-胺进行乙酰化。 还提供了包含食物来源,饲料和补充剂的组合物,以及增加植物,植物部分,种子或谷物的营养价值的方法。