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    • 33. 发明授权
    • Juvenile hormone or one of its agonists as a chemical ligand to control gene expression in plants by receptor mediated transactivation
    • 少年激素或其激动剂之一作为通过受体介导的反式激活控制植物中的基因表达的化学配体
    • US06362394B1
    • 2002-03-26
    • US09376107
    • 1999-08-17
    • Lyle Dean CrosslandStephen Arthur Goff
    • Lyle Dean CrosslandStephen Arthur Goff
    • C12N1582
    • C12N15/8238C07K14/70567C07K14/721C07K2319/00C12N15/8287
    • The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises transforming a plant with a USP receptor expression cassette which encodes a USP receptor and at least one target expression cassette which encodes a target polypeptide. Contacting said transformed plant with juvenile hormone or one of its agonists activates expression of the target polypeptide in the presence of said USP receptor polypeptide. Optionally, additional “secondary” receptor expression cassettes may be used, wherein the secondary receptor expression cassette encodes a receptor polypeptide distinct from USP. The method is useful for controlling various traits of agronomic importance, such as plant fertility. Also disclosed is a method of identifying previously unknown ligands for USP. Substances to be tested are identified by placing them in contact with plant cells transformed with a USP receptor expression cassette and a target expression cassette. The target expression cassette encodes a reporter polypeptide whose expression can be determined quantitatively or qualitatively, whereby the test substance is identified as a ligand for USP.
    • 本发明涉及控制植物中基因表达的方法。 具体地,该方法包括用编码USP受体的USP受体表达盒和至少一个编码靶多肽的目标表达盒转化植物。 将所述转化的植物与幼体激素或其一种激动剂接触在所述USP受体多肽的存在下激活靶多肽的表达。 任选地,可以使用另外的“次级”受体表达盒,其中二级受体表达盒编码与USP不同的受体多肽。 该方法可用于控制农艺学重要性的各种性状,如植物生育力。 还公开了鉴定USP以前未知的配体的方法。 通过将它们与用USP受体表达盒和目标表达盒转化的植物细胞接触来鉴定待测物质。 靶表达盒编码报告多肽,其表达可以定量或定性地测定,由此将检测物质鉴定为USP的配体。