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31. 发明授权

US5145783A Glyphosate-tolerant 5-endolpyruvyl-3-phosphoshikimate synthase 失效
标题翻译：耐草甘膦5-端烯丙基呋喃-3-磷酸合成酶
公开(公告)号：US5145783A
公开(公告)日：1992-09-08
申请号：US550276
申请日：1990-07-09
申请人： Ganesh M. Kishore , Dilip M. Shah
发明人： Ganesh M. Kishore , Dilip M. Shah
IPC分类号： C12N9/10 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/10
摘要： Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a conserved sequence found between positions 80 and 120 in the mature wild-type EPSP synthase.
摘要翻译：编码耐草甘膦的5-烯醇丙酮酸-3-磷酸鸟苷酸（EPSP）合成酶，编码耐受草甘膦的EPSP合成酶的DNA，编码草甘膦耐受酶的植物基因，含有该基因的植物转化载体，转化的植物细胞和含有植物基因的分化转化植物被披露。通过在成熟野生型EPSP合酶中在位置80和120之间发现的保守序列中取代丙氨酸残基的甘氨酸残基来制备草甘膦耐受性EPSP合成酶。

32. 发明授权

US09562251B2 Production of steviol glycosides in microorganisms 有权
标题翻译：微生物中甜菊糖苷的生产
公开(公告)号：US09562251B2
公开(公告)日：2017-02-07
申请号：US13701406
申请日：2011-06-02
申请人： Ganesh M. Kishore , Jorgen Hansen , Jens Houghton-Larsen , Esben Halkjær Hansen , Michael Dalgaard Mikkelsen , Sabina Tavares , Charlotte Blom
发明人： Ganesh M. Kishore , Jorgen Hansen , Jens Houghton-Larsen , Esben Halkjær Hansen , Michael Dalgaard Mikkelsen , Sabina Tavares , Charlotte Blom
IPC分类号： C12P19/56 , C12P7/42 , C12N9/10 , C12N15/52 , C12N15/82 , C12P15/00
CPC分类号： C12P19/56 , A23L27/36 , C12N9/1051 , C12N9/1077 , C12N15/52 , C12N15/8243 , C12N15/8245 , C12P7/42 , C12P15/00 , C12Y204/01126 , Y02P20/52
摘要： Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.
摘要翻译：公开了重组微生物，植物和植物细胞，其被工程化以表达编码甜菊醇生物合成酶和UDP-糖基转移酶（UGT）的新型重组基因。这样的微生物，植物或植物细胞可以产生甜菊醇或甜菊糖苷，例如可以在食品和膳食补充剂中用作天然甜味剂的罗红糖苷或雷鲍迪苷A.

33. 发明申请

US20110166224A1 DIACYLGLYCEROL RICH FATS, OILS AND FUNCTIONAL FOODS 审中-公开
标题翻译： DIACYLGLYCEROL富含脂肪，油和功能食品
公开(公告)号：US20110166224A1
公开(公告)日：2011-07-07
申请号：US12981302
申请日：2010-12-29
申请人： Ganesh M. Kishore , Riccardo G. Locascio
发明人： Ganesh M. Kishore , Riccardo G. Locascio
IPC分类号： A61K31/23 , A23D9/00 , A21D13/00 , A21D10/00 , A21D13/08 , A23C9/123 , A23L1/187 , A23C19/00 , A23D7/00 , A23C15/12 , A23L1/24 , A61P9/12 , A61P9/00 , A61P3/10 , A61P3/00 , A61P3/06 , A61P9/10
CPC分类号： A21D2/16 , A21D2/165 , A23C9/1315 , A23C15/126 , A23C19/093 , A23D7/011 , A23D7/013 , A23D9/013 , A23L7/126 , A23L25/10 , A23L33/11 , A23L33/115 , A23L33/12 , A23L33/30 , A61K31/23 , A61K31/231 , A61K45/06 , A61K2300/00
摘要： Disclosed is a fat or oil useful for cooking applications which includes from 10 to 90% by weight diacylglycerols, and has a SFI of at least 15% at room temperature. In particular embodiments, the fat or oil is derived from palm oil, palm kernel oil, coconut oil, sunflower oil, soybean oil, corn oil, rapeseed oil, grape seed oil, rice bran oil, sesame oil, and peanut oil, or any combination thereof. In some cases, the fat or oil exhibits health benefits including lowered serum LDL, raised serum HDL, lowered total serum cholesterol, reduced risk of metabolic syndrome, reduced risk of diabetes mellitus, enhanced fetal health, enhanced insulin sensitivity, reduced risk of hypertension, and enhanced resistance to obesity per unit of consumption. Food compositions and methods of health enhancement utilizing the fats and oils of the invention are also disclosed.
摘要翻译：公开了一种用于烹饪应用的脂肪或油，其包含10至90重量％的二酰基甘油，并且在室温下具有至少15％的SFI。在具体实施方案中，脂肪或油衍生自棕榈油，棕榈仁油，椰子油，向日葵油，大豆油，玉米油，菜籽油，葡萄籽油，米糠油，芝麻油和花生油，或任何的组合。在某些情况下，脂肪或油会显示健康益处，包括降低血清LDL，升高血清HDL，降低总血清胆固醇，降低代谢综合征风险，降低糖尿病风险，增强胎儿健康，增强胰岛素敏感性，降低高血压风险，并增强每单位消费对肥胖的抵抗力。还公开了利用本发明的脂肪和油的健康增强的食物组合物和方法。

34. 发明授权

US07906710B2 Transgenic plants containing altered levels of steroid compounds 有权
标题翻译：含有改变水平的类固醇化合物的转基因植物
公开(公告)号：US07906710B2
公开(公告)日：2011-03-15
申请号：US12477673
申请日：2009-06-03
申请人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译：公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。还公开了使用这种构建体来改变植物的细胞，植物，种子和储存器官中的固醇产生和含量的方法。还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。还提供了可用于改变甾醇生产的新型核苷酸序列。还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶，至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞，植物，种子和储存器官。

35. 发明申请

US20100037354A1 TRANSGENIC PLANTS CONTAINING ALTERED LEVELS OF STEROID COMPOUNDS 有权
标题翻译：含有替代化合物水平的转基因植物
公开(公告)号：US20100037354A1
公开(公告)日：2010-02-11
申请号：US12477673
申请日：2009-06-03
申请人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
IPC分类号： A01H5/00 , C12N5/04
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译：公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。还公开了使用这种构建体来改变植物的细胞，植物，种子和储存器官中的固醇产生和含量的方法。还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。还提供了可用于改变甾醇生产的新型核苷酸序列。还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶，至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞，植物，种子和储存器官。

36. 发明授权

US06723897B2 Methods for controlling gibberellin levels 有权
标题翻译：控制赤霉素水平的方法
公开(公告)号：US06723897B2
公开(公告)日：2004-04-20
申请号：US09371307
申请日：1999-08-10
申请人： Sherri M. Brown , Tedd D. Elich , Gregory R. Heck , Ganesh M. Kishore , Eugene W. Logusch , Sherry J. Logusch , Kenneth J. Piller , Sudabathula Rao , Joel E. Ream , Scott R. Baerson
发明人： Sherri M. Brown , Tedd D. Elich , Gregory R. Heck , Ganesh M. Kishore , Eugene W. Logusch , Sherry J. Logusch , Kenneth J. Piller , Sudabathula Rao , Joel E. Ream , Scott R. Baerson
IPC分类号： C12N1582
CPC分类号： C12N9/0071 , C12N9/00 , C12N9/0069 , C12N15/8222 , C12N15/8243 , C12N15/8297
摘要： Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase, 3-&bgr; hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing antisense RNA which suppresses the gibberellin biosynthetic pathway sequence. The antisense sequence is the complement of a copalyl diphosphate synthase sequence, a 3&bgr;-hydroxylase sequence, or a C20-oxidase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.
摘要翻译：公开了用于抑制和控制赤霉酸水平的方法和材料。具体地说，公开了二甲基二磷酸合成酶，3-β羟化酶和2-氧化酶的核酸序列和另外的核酸序列。可以通过制备能够表达抑制赤霉素生物合成途径序列的反义RNA的嵌合表达构建体来抑制或控制赤霉酸水平。反义序列是双酰基二磷酸合成酶序列，3β-羟化酶序列或C20-氧化酶序列的补体。补充剂，优选赤霉素或赤霉素前体或中间体的施用恢复生物活性。

37. 发明授权

US5627061A Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases 失效
标题翻译：耐草甘膦5-烯醇丙酮酸莽草酸-3-磷酸合成酶
公开(公告)号：US5627061A
公开(公告)日：1997-05-06
申请号：US476008
申请日：1995-06-07
申请人： Gerard F. Barry , Ganesh M. Kishore , Stephen R. Padgette , William C. Stallings
发明人： Gerard F. Barry , Ganesh M. Kishore , Stephen R. Padgette , William C. Stallings
IPC分类号： C12N9/10 , C12N15/54 , C12N15/82
CPC分类号： C12N9/1092 , C12N15/8275 , Y10S435/816 , Y10S435/822 , Y10S435/839 , Y10S435/874 , Y10S435/883 , Y10T436/143333
摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
摘要翻译：公开了编码II类EPSPS酶的基因。这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

38. 发明授权

US5512466A Control of fruit ripening and senescence in plants 失效
标题翻译：控制植物中的果实成熟和衰老
公开(公告)号：US5512466A
公开(公告)日：1996-04-30
申请号：US809457
申请日：1991-12-17
申请人： Harry J. Klee , Ganesh M. Kishore
发明人： Harry J. Klee , Ganesh M. Kishore
IPC分类号： A01H5/00 , C07H21/04 , C12N9/88 , C12N15/09 , C12N15/60 , C12N15/82 , C12N15/31 , A01H5/08 , C12N15/63
CPC分类号： C12N15/8249 , C12N9/88
摘要： A method for controlling the ripening of fruits and vegetables as well as a method for controlling senescence of plant tissue is described. The method generally embraces the expression of an ACC metabolizing enzyme in the fruit or other desired plant tissue to inhibit the production of ethylene in the fruit or plant tissue. The use of the ACC metabolizing enzyme ACC deaminase is described in detail. The ripening or senescence process in the fruit or plant tissue is inhibited by the expression of the ACC deaminase gene such that the shelf-life and marketability of the fruit or plant is enhanced. The ACC metabolizing enzyme may be used in combination with other methods for reducing ethylene production in transformed plants to further reduce the production of ethylene in the fruit or plant. DNA constructs containing the ACC deaminase gene are also described.
摘要翻译：描述了控制水果和蔬菜成熟的方法以及控制植物组织衰老的方法。该方法通常包含ACC代谢酶在果实或其它所需植物组织中的表达，以抑制水果或植物组织中的乙烯的产生。 ACC代谢酶ACC脱氨酶的使用详细描述。水果或植物组织中的成熟或衰老过程受到ACC脱氨酶基因的表达的抑制，使得水果或植物的保质期和市售性提高。 ACC代谢酶可以与其他减少转化植物中乙烯生产的方法结合使用，以进一步减少水果或植物中乙烯的产生。还描述了含有ACC脱氨酶基因的DNA构建体。

39. 发明授权

US5463175A Glyphosate tolerant plants 失效
标题翻译：草甘膦耐受性植物
公开(公告)号：US5463175A
公开(公告)日：1995-10-31
申请号：US391339
申请日：1995-02-21
申请人： Gerard F. Barry , Ganesh M. Kishore
发明人： Gerard F. Barry , Ganesh M. Kishore
IPC分类号： A01H5/00 , C07H21/04 , C12N1/20 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/06 , C12N15/00 , C12N15/09 , C12N15/53 , C12N15/82 , C12P21/06 , C12R1/01 , C12R1/38 , C12R1/91 , C12N5/14
CPC分类号： C12R1/38 , C12N15/8275 , C12N9/0004 , C12N9/0012 , C12R1/01
摘要： Genes encoding a glyphosate oxidoreductase enzyme are disclosed. The genes are useful in producing transformed bacteria and plants which degrade glyphosate herbicide as well as crop plants which are tolerant to glyphosate herbicide.
摘要翻译：公开了编码草甘膦氧化还原酶的基因。这些基因可用于生产转化的细菌和植物，其降解草甘膦除草剂以及对草甘膦除草剂具有耐受性的作物植物。

40. 发明授权

US5310667A Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases 失效
标题翻译：耐草甘膦5-烯醇丙酮酸-3-磷酸羟肟酸合成酶
公开(公告)号：US5310667A
公开(公告)日：1994-05-10
申请号：US380963
申请日：1989-07-17
申请人： David A. Eichholtz , Charles S. Gasser , Ganesh M. Kishore
发明人： David A. Eichholtz , Charles S. Gasser , Ganesh M. Kishore
IPC分类号： C12N9/10 , C12N15/54 , C12N15/82 , C12N15/01 , C12N15/29 , C12N15/32
CPC分类号： C12N9/1092 , C12N15/8275
摘要： Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a first conserved sequence found between positions 80 and 120, and either an aspartic acid residue or asparagine residue for a glycine residue in a second conserved sequence found between positions 120 and 160 in the mature wild type EPSP synthase.
摘要翻译：编码耐草甘膦的5-烯醇丙酮酸-3-磷酸鸟苷酸（EPSP）合成酶，编码耐受草甘膦的EPSP合成酶的DNA，编码草甘膦耐受酶的植物基因，含有该基因的植物转化载体，转化的植物细胞和含有植物基因的分化转化植物被披露。通过在第80和120位之间发现的第一个保守序列中取代丙氨酸残基作为甘氨酸残基，并且在位置之间发现的第二个保守序列中的甘氨酸残基的天冬氨酸残基或天冬酰胺残基取代了草甘膦耐受EPSP合成酶成熟野生型EPSP合酶中的120和160。

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