专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

31. 发明授权

US10066265B2 Determining antigen-specific t-cells 有权
公开(公告)号：US10066265B2
公开(公告)日：2018-09-04
申请号：US14242520
申请日：2014-04-01
申请人： Adaptive Biotechnologies Corp.
发明人： Mark Klinger , Malek Faham
IPC分类号： C12Q1/6881
CPC分类号： C12Q1/6881 , C12Q2600/106 , C12Q2600/158 , C12Q2600/16
摘要： The invention is directed to methods for determining antigen-specific T cells. In some embodiments, methods of the invention may be implemented by the steps of reacting under interaction conditions one or more antigens with T cells in a plurality of subsets of a tissue sample, such as peripheral blood; sorting antigen-interacting T cells from other T cells; separately sequencing for each subset recombined nucleic acid encoding a segment of a TCR chain from a sample of T cells prior to exposure to antigen and from a sample of T cells isolated based on their interaction with antigen, thereby forming a clonotype profile for the former sample and the latter sample for each subset; and identifying as antigen-specific T cells those T cells associated with a clonotype whose frequency increases in the latter sample relative to its frequency in the former sample.

32. 发明申请

US20180112278A1 METHODS OF MONITORING CONDITIONS BY SEQUENCE ANALYSIS 审中-公开
公开(公告)号：US20180112278A1
公开(公告)日：2018-04-26
申请号：US15820655
申请日：2017-11-22
申请人： Adaptive Biotechnologies Corp.
发明人： Malek FAHAM , Thomas WILLIS
IPC分类号： C12Q1/6886 , C12Q1/6869 , C12Q1/6883 , C12Q1/6827 , C12Q1/6881 , C12N15/10
CPC分类号： C12Q1/6886 , C12N15/1072 , C12Q1/6809 , C12Q1/6827 , C12Q1/6869 , C12Q1/6881 , C12Q1/6883 , C12Q2600/106 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , Y02A90/26
摘要： There is a need for improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer. Provided herein are methods for using DNA sequencing to identify personalized biomarkers in patients with autoimmune disease and other conditions. Identified biomarkers can be used to determine the disease state for a subject with an autoimmune disease or other condition.

33. 发明申请

US20180080078A1 METHOD OF IDENTIFYING HUMAN COMPATIBLE T CELL RECEPTORS SPECIFIC FOR AN ANTIGENIC TARGET 审中-公开
公开(公告)号：US20180080078A1
公开(公告)日：2018-03-22
申请号：US15563365
申请日：2016-04-01
申请人： ADAPTIVE BIOTECHNOLOGIES CORP. , FRED HUTCHINSON CANCER RESEARCH CENTER
发明人： Harlan S. ROBINS , Aude Georgiana CHAPUIS , Thomas M. SCHMITT , Philip GREENBERG , Anna SHERWOOD
IPC分类号： C12Q1/68 , G01N33/68 , G01N33/569
CPC分类号： C12Q1/6881 , C12N5/0636 , C12Q1/6804 , G01N33/56977 , G01N33/6872 , G01N2333/7051 , G01N2333/70539 , C12Q1/6869 , C12Q2531/113 , C12Q2537/143 , C12Q1/6806
摘要： Methods are provided for identifying T cell receptors that specifically bind a particular antigenic target and can be used as therapeutics against disease.

34. 发明申请

US20180037953A1 METHODS FOR DIAGNOSING INFECTIOUS DISEASE AND DETERMINING HLA STATUS USING IMMUNE REPERTOIRE SEQUENCING 审中-公开
公开(公告)号：US20180037953A1
公开(公告)日：2018-02-08
申请号：US15553040
申请日：2016-02-24
申请人： Adaptive Biotechnologies Corp. , Fred Hutchinson Cancer Research Center
发明人： Ryan O. Emerson , Harlan S. Robins , Mark Rieder , William Dewitt, III , Christopher Carlson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/68 , C12Q1/6869 , C12Q1/6883 , C12Q1/70 , C12Q2600/158 , C12Q2600/172
摘要： Methods are provided for predicting a subject's infection status using high-throughput T cell receptor sequencing to match the subject's TCR repertoire to a known set of disease-associated T cell receptor sequences. The methods of the present invention may be used to predict the status of several infectious agents in a single sample from a subject. Methods are also provided for predicting a subject's HLA status using high-throughput immune receptor sequencing.

35. 发明申请

US20180030543A1 IMMUNOCOMPETENCE ASSESSMENT BY ADAPTIVE IMMUNE RECEPTOR DIVERSITY AND CLONALITY CHARACTERIZATION 审中-公开
公开(公告)号：US20180030543A1
公开(公告)日：2018-02-01
申请号：US15637871
申请日：2017-06-29
申请人： Adaptive Biotechnologies Corp. , Memorial Sloan-Kettering Cancer Center
发明人： Harlan S. Robins , Julie Rubinstein , Ryan Emerson , Jianda Yuan
IPC分类号： C12Q1/68 , G06F19/22 , G06F19/12
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6883 , C12Q2600/106 , C12Q2600/156 , C12Q2600/16 , G06F19/12 , G06F19/22 , C12Q2537/143
摘要： Disclosed are methods for determining the immunological status of the adaptive immune system of a subject by identifying and quantifying rearranged DNA (and/or subsequently transcribed RNA) sequences encoding T cell receptor (TCR) and/or immunoglobulin (IG) polypeptides, in a lymphoid DNA-containing sample from the subject TCR and/or IG sequence diversity and sequence distribution permit immunocompetence and immune repertoire assessment and reflect the degree of T cell or B cell clonality and clerical exspansion in the sample. Methods for stratifying patient populations on the basis of immunocompetence including likelihood of responding to immunotherapy are also described.

36. 发明申请

US20180023143A9 MONITORING HEALTH AND DISEASE STATUS USING CLONOTYPE PROFILES 审中-公开
公开(公告)号：US20180023143A9
公开(公告)日：2018-01-25
申请号：US15061750
申请日：2016-03-04
申请人： ADAPTIVE BIOTECHNOLOGIES CORP.
发明人： Malek FAHAM , Thomas Willis
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6881 , C12Q1/6883 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16
摘要： There is a need for improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer, especially lymphoid neoplasms, such as lymphomas and leukemias. Provided herein are methods for using DNA sequencing to identify personalized, or patient-specific biomarkers in patients with lymphoid neoplasms, autoimmune disease and other conditions. Identified biomarkers can be used to determine and/or monitor the disease state for a subject with an associated lymphoid disorder or autoimmune disease or other condition. In particular, the invention provides a sensitive method for monitoring lymphoid neoplasms that undergo clonal evolutions without the need to development alternative assays for the evolved or mutated clones serving as patient-specific biomarkers.

37. 发明授权

US09523129B2 Sequence analysis of complex amplicons 有权
标题翻译：复合扩增子的序列分析
公开(公告)号：US09523129B2
公开(公告)日：2016-12-20
申请号：US14173712
申请日：2014-02-05
申请人： Adaptive Biotechnologies Corp.
发明人： Malek Faham , Martin Moorhead , Thomas Willis
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q1/6874 , C12Q1/6881 , C12Q1/6883 , C12Q2600/112 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16
摘要： The invention is directed to methods of generating sequence profiles of populations of nucleic acids, whose member nucleic acids contain regions of high variability, such as populations of nucleic acids encoding T cell receptors or B cell receptors. In one aspect, the invention provides pluralities of sets of primers for generating nested sets of templates from nucleic acids in such populations, thereby insuring the production of at least one template from which sequence reads are generated, despite such variability, or despite limited lengths or quality of sequence reads. In another aspect, members of such populations are bidirectionally sequenced so that further sequence information is obtained by analyzing overlapping sequence reads in the zones of highest variability.
摘要翻译：本发明涉及产生核酸群体序列谱的方法，该核酸的成员核酸含有高度变异性的区域，例如编码T细胞受体或B细胞受体的核酸群体。在一个方面，本发明提供了多组引物，用于从这些群体中的核酸产生嵌套的模板集合，从而确保产生序列读数的至少一个模板的产生，尽管这种变化性，或者尽管有限的长度或序列读取的质量。在另一方面，这些群体的成员被双向排序，以便通过分析最高变异性区域中的重叠序列读数来获得进一步的序列信息。

38. 发明申请

US20160340729A1 METHODS FOR DEFINING AND PREDICTING IMMUNE RESPONSE TO ALLOGRAFT 审中-公开
标题翻译：用于定义和预测免疫应答对ALLOGRAFT的方法
公开(公告)号：US20160340729A1
公开(公告)日：2016-11-24
申请号：US15110589
申请日：2015-01-09
申请人： ADAPTIVE BIOTECHNOLOGIES CORP. , NORTHWESTERN UNIVERSITY
发明人： Ryan O. Emerson , Ilan Kirsch , James Mathew , Joseph Leventhal
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q2600/118 , C12Q2600/156
摘要： Methods are provided for predicting and determining a subject's immune response to allograft. Methods include assessing immune response to an allograft by characterizing the diversity and distribution of clones of the adaptive immune repertoire. Methods are also provided for characterizing the adaptive immune response of a subject to an allograft using a mixed lymphocyte reaction culture.
摘要翻译：提供了用于预测和确定受试者对同种异体移植物的免疫应答的方法。方法包括通过表征适应性免疫谱的克隆的多样性和分布来评估同种异体移植物的免疫应答。还提供了使用混合淋巴细胞反应培养物来表征受试者对同种异体移植物的适应性免疫应答的方法。

39. 发明授权

US09499865B2 Detection and measurement of tissue-infiltrating lymphocytes 有权
标题翻译：组织浸润淋巴细胞的检测和测量
公开(公告)号：US09499865B2
公开(公告)日：2016-11-22
申请号：US13688414
申请日：2012-11-29
申请人： Adaptive Biotechnologies Corp.
发明人： Malek Faham , Mark Klinger
IPC分类号： C12Q1/68 , C12Q1/06 , C12P19/34 , G01N33/50
CPC分类号： C12Q1/6881 , C12Q1/06 , G01N33/5091 , G01N33/5094
摘要： The present invention is drawn to methods for measuring numbers, levels, and/or ratios of cells, such as lymphocytes, infiltrated into a solid tissue, such as a tumor or a tissue affected by an autoimmune disease, and to methods for making patient prognoses based on such measurements. In one aspect, methods of the invention comprise sorting lymphocytes from an accessible tissue, such as peripheral blood, into functional subsets, such as cytotoxic T cells and regulatory T cells, and generating clonotype profiles of each subset. An inaccessible disease-affected tissue is sampled and one or more clonotype profiles are generated. From the latter clonotype profiles, levels lymphocytes in each of the functional subsets are determined in the disease-affected tissue by their clonotypes, which are identified from lymphocytes sorted into subsets from the accessible tissue.
摘要翻译：本发明涉及用于测量浸润到固体组织例如肿瘤或受自身免疫性疾病影响的组织的细胞例如淋巴细胞的数量，水平和/或比率的方法，以及用于产生患者预后的方法基于这样的测量。在一个方面，本发明的方法包括将淋巴细胞从诸如外周血的可及组织分选成功能性亚类，例如细胞毒性T细胞和调节性T细胞，以及产生每个子集的克隆型谱。对不能接近的疾病影响的组织进行采样，并产生一个或多个克隆型谱。从后一种克隆型谱中，通过其克隆型在疾病影响组织中测定每个功能亚类中的淋巴细胞水平，所述克隆型从从可及组织分类为亚群的淋巴细胞中鉴定。

40. 发明申请

US20150247182A1 SINGLE CELL ANALYSIS USING SEQUENCE TAGS 审中-公开
标题翻译：使用序列标签的单细胞分析
公开(公告)号：US20150247182A1
公开(公告)日：2015-09-03
申请号：US14425036
申请日：2013-07-22
申请人： ADAPTIVE BIOTECHNOLOGIES CORP.
发明人： Malek Faham , Thomas Willis , Jianbiao Zheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q2521/301 , C12Q2527/101 , C12Q2563/159 , C12Q2563/179
摘要： The invention provides a method of making measurements on individual cells of a population by forming reactors containing single cells and a predetermined number, usually one, homogeneous sequence tag. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences, such as sequence tag copies derived from a homogeneous sequence tag, to other cellular nucleic acids of interest, thereby forming fusion products. The fusion products of such PCA reactions are then sequenced and tabulated to generate multiparameter data for cells of the population.
摘要翻译：本发明提供了通过形成含有单个细胞的反应器和预定数量的通常为一个均质序列标签来对群体的个体细胞进行测量的方法。一方面，本发明提供了一种通过进行聚合酶循环组件（PCA）反应以连接其鉴定的核酸序列（例如衍生自均质序列标签的序列标签拷贝）来对这样群体的个体细胞进行多参数测量的方法，到目的地的其他细胞核酸，从而形成融合产物。然后对这种PCA反应的融合产物进行测序并列表，以产生群体细胞的多参数数据。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式