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21. 发明申请

US20170175078A1 CELL CULTURE SUBSTRATE COMPRISING FLUORINE-CONTAINING POLYMER ON ITS SURFACE 审中-公开
公开(公告)号：US20170175078A1
公开(公告)日：2017-06-22
申请号：US15304910
申请日：2015-03-17
申请人： Nippon Shokubai, Co., Ltd.
发明人： Tomomi Makino , Hiroshi Horikawa
IPC分类号： C12N5/00 , C08G65/40 , C08J5/18 , C08G73/10 , C12N5/071 , C12M1/12
CPC分类号： C12N5/0062 , C08G65/4043 , C08G73/10 , C08G73/1028 , C08G73/1039 , C08G73/105 , C08G73/1071 , C08J5/18 , C08J2371/10 , C08J2379/08 , C12M23/20 , C12M23/24 , C12M23/26 , C12M25/02 , C12N5/0068 , C12N5/0671 , C12N2513/00 , C12N2533/30 , C12N2539/00
摘要： This invention provides a cell culture substrate comprising on its surface a fluorine-containing polymer that enables three-dimensional tissue culture. The cell culture substrate of the invention has a surface at least a part of which is composed of a resin composition comprising a fluorine-containing polymer having one or more fluorine atoms in a repeating unit and exhibits the oxygen gas permeability of 219 cm3 (STP)/(m2·24 h·atm) or higher. Three-dimensional tissue can be formed via cell culture with the use of the cell culture substrate of the invention.

22. 发明申请

US20170037364A1 METHOD OF PREPARING CELLS FOR 3D TISSUE CULTURE 审中-公开
标题翻译：制备三维组织培养细胞的方法
公开(公告)号：US20170037364A1
公开(公告)日：2017-02-09
申请号：US15303455
申请日：2015-04-15
申请人： INSPHERO AG
发明人： Simon MESSNER , Wolfgang MORITZ , Jan LICHTENBERG , Jens M. KELM
IPC分类号： C12N5/00 , G01N33/50 , C12N5/071
CPC分类号： C12N5/0062 , C12N5/0068 , C12N5/0671 , C12N2513/00 , C12N2533/00 , G01N33/5014 , G01N33/5067
摘要： The present invention relates to a method of preparing cells for 3D tissue culture, which method comprises the steps of plating the cells on a suitable surface, optionally, checking for their capability to adhere to said surface, discarding the cells which have not adhered to said surface, detaching the adhered cells and transferring them into a 3D tissue culture process.
摘要翻译：本发明涉及一种制备3D组织培养细胞的方法，该方法包括以下步骤：在合适的表面上电镀细胞，任选地检查其粘附到所述表面的能力，将未附着于所述细胞的细胞丢弃表面，分离粘附的细胞并将其转移到3D组织培养过程中。

23. 发明申请

US20170015974A1 BIOMATRIX SCAFFOLDS 审中-公开
公开(公告)号：US20170015974A1
公开(公告)日：2017-01-19
申请号：US15221413
申请日：2016-07-27
申请人： The University of North Carolina at Chapel Hill
发明人： Yunfang WANG , Lola Cynthia McAdams REID , Mitsuo YAMAUCHI , Cai-Bin CUI , Andrew Zhuang WANG , Michael Edward WERNER
IPC分类号： C12N5/00 , A61L27/24 , A61L27/36 , C12N7/00 , C12N5/071
CPC分类号： C12N5/0068 , A61L27/24 , A61L27/3604 , A61L27/3683 , A61L27/3691 , A61L2430/28 , C12N5/067 , C12N5/0671 , C12N5/0693 , C12N7/00 , C12N2500/25 , C12N2500/36 , C12N2501/20 , C12N2501/998 , C12N2506/14 , C12N2533/54 , C12N2533/90
摘要： The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.
摘要翻译：本发明提供生物基质支架，富集细胞外基质组分的组织提取物和结合的生长因子，细胞因子和激素，以及制备和使用它们的方法。

24. 发明申请

US20160298087A1 LIVER-MIMETIC DEVICE AND METHOD FOR SIMULATION OF HEPATIC FUNCTION USING SUCH DEVICE 审中-公开
标题翻译：肝脏装置和使用这种装置模拟肝功能的方法
公开(公告)号：US20160298087A1
公开(公告)日：2016-10-13
申请号：US14895912
申请日：2014-06-04
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： Xin QU , Maling GOU , Wei ZHU , Shaochen CHEN
IPC分类号： C12N5/071
CPC分类号： C12N5/0671 , C12N2506/45 , C12N2533/30 , C12N2533/54 , C12N2533/80
摘要： A liver-mimetic device and method include a 3D polymer scaffold having a matrix of liver-like lobules with hepatic-functioning particles encapsulated within the lobules. In some embodiments, each liver-like lobule is hexagonal in structure and the matrix is in a honeycomb arrangement. In some embodiments, the hepatic-functioning particles are hepatic progenitor cells. In other embodiments, the hepatic-functioning particles are polymer nanoparticles adapted to capture pore-forming toxins.
摘要翻译：肝模拟装置和方法包括具有包封在小叶内的肝功能颗粒的肝样小叶的基质的3D聚合物支架。在一些实施方案中，每个肝样小叶的结构是六边形的，并且基质是蜂窝状的。在一些实施方案中，肝功能颗粒是肝祖细胞。在其它实施方案中，肝功能颗粒是适于捕获成孔毒素的聚合物纳米颗粒。

25. 发明授权

US09442105B2 Engineered liver tissues, arrays thereof, and methods of making the same 有权
标题翻译：工程肝组织，其阵列及其制备方法
公开(公告)号：US09442105B2
公开(公告)日：2016-09-13
申请号：US13841430
申请日：2013-03-15
申请人： Organovo, Inc.
发明人： Benjamin R. Shepherd , Justin B. Robbins , Vivian A. Gorgen , Sharon C. Presnell
IPC分类号： C12N5/071 , G01N33/50 , C12N5/077
CPC分类号： C12N5/0697 , C12N5/0656 , C12N5/0671 , C12N2502/14 , C12N2502/28 , C12N2533/30 , C12N2535/10 , G01N33/5008 , G01N33/5088
摘要： Engineered, living, three-dimensional liver tissue constructs comprising: one or more layers, wherein each layer contains one or more liver cell types, the one or more layers cohered to form a living, three-dimensional liver tissue construct. In some embodiments, the constructs are characterized by having at least one of: at least one layer comprising a plurality of cell types, the cell types spatially arranged relative to each other to create a planar geometry; and a plurality of layers, at least one layer compositionally or architecturally distinct from at least one other layer to create a laminar geometry. Also disclosed are arrays and methods of making the same. Also disclosed are engineered, living, three-dimensional liver tissue constructs for use in the augmentation or restoration of one or more liver functions, by in vivo delivery of tissue or utilization of tissue in an extracorporeal device.
摘要翻译：包括：一层或多层，其中每层含有一种或多种肝细胞类型的工程化的，活的三维肝组织构建体，所述一层或多层被粘结以形成活的三维肝组织构建体。在一些实施方案中，构建体的特征在于具有以下至少一个：包含多个细胞类型的至少一个层，所述细胞类型相对于彼此空间布置以产生平面几何形状; 以及多个层，至少一个层与至少一个其它层组成或结构上不同以产生层状几何形状。还公开了制造它们的阵列和方法。还公开了用于增加或恢复一种或多种肝功能的工程化的，活的三维肝组织构建体，通过体外递送组织或在体外装置中利用组织。

26. 发明申请

US20160215267A1 METHODS FOR GENERATING 3D CELL CULTURE MODEL IN HIGH RESPIRATION ENVIRONMENTS FOR HIGH-CONTENT ANALYSES AND SCREENING 审中-公开
标题翻译：用于高分辨率分析和筛选的高分辨率环境中生成3D细胞培养模型的方法
公开(公告)号：US20160215267A1
公开(公告)日：2016-07-28
申请号：US14604275
申请日：2015-01-23
申请人： IKKO-ZU CORPORATION , KYOTO UNIVERSITY
发明人： Jun Kanamune , Shoichiro Sumi , Kazuyuki Yokokawa , Hideki Matsunaga , Chongmun Kim
IPC分类号： C12N5/071
CPC分类号： C12N5/0671 , C12N2500/02 , C12N2500/34 , C12N2503/02 , C12N2503/04 , C12N2533/54
摘要： A method of culturing cells to form cell aggregations and subjecting the cell aggregations to imaging analysis, comprises: seeding cells in a culture medium on a substantially flat substrate that is transparent, oxygen gas-permeable and liquid impermeable and is coated with atelo-collagen of large-mammalian origin; culturing the cells by continuously supplying air or oxygen-containing gas to the cells through said substrate to form the cell aggregations that are three-dimensional (3D), shaped as undulated as islands or mountains and discretely arranged as islands separated by substantially cell-free gaps; subjecting the cell aggregations on said substrate to the imaging analysis by using lights passing through said substrate.
摘要翻译：培养细胞以形成细胞聚集并使细胞聚集体进行成像分析的方法包括：将培养基中的细胞在透明的，透气性的和液体不可渗透的基本平坦的基质上接种，并涂覆有大哺乳动物来源通过连续向细胞提供空气或含氧气体通过所述基质培养细胞以形成三维（3D）的细胞聚集体，其形状如岛状或山脉形成为波状，并且离散地布置成通过基本上无细胞分离的岛差距通过使用通过所述基板的光使所述基板上的细胞聚集体进行成像分析。

27. 发明申请

US20160137984A1 COMPOSITIONS AND METHODS OF FUNCTIONALLY ENHANCED IN VITRO CELL CULTURE SYSTEM 审中-公开
标题翻译：在体细胞培养系统中功能增强的组合物和方法
公开(公告)号：US20160137984A1
公开(公告)日：2016-05-19
申请号：US15002292
申请日：2016-01-20
申请人： Martin Yarmush , Robert Freedman , Yaakov Nahmias , Eric Novik
发明人： Martin Yarmush , Robert Freedman , Yaakov Nahmias , Eric Novik
IPC分类号： C12N5/071
CPC分类号： C12N5/0671 , C12M23/12 , C12M23/16 , C12M25/14 , C12M35/08 , C12N2500/02 , C12N2500/90 , C12N2513/00
摘要： Compositions and methods described herein provide a cell culture system in which cells are in high metabolic states from the onset of the culture. Combinations of various cell culture components disclosed and employed herein allow cells to be in high metabolic states useful for drug testing immediately after the start of cell culture.
摘要翻译：本文描述的组合物和方法提供细胞培养系统，其中细胞在培养起始时处于高代谢状态。本文公开和使用的各种细胞培养组分的组合允许细胞在细胞培养开始之后立即处于用于药物测试的高代谢状态。

28. 发明授权

US09273276B2 Devices and methods for pharmacokinetic-based cell culture system 有权
标题翻译：基于药物动力学的细胞培养系统的装置和方法
公开(公告)号：US09273276B2
公开(公告)日：2016-03-01
申请号：US13301261
申请日：2011-11-21
申请人： Michael Shuler , Gregory T. Baxter , Aaron Sin , Robert Andrew Harrison , Scott Meyers
发明人： Michael Shuler , Gregory T. Baxter , Aaron Sin , Robert Andrew Harrison , Scott Meyers
IPC分类号： C12M1/34 , C12M3/06 , C12M3/00 , C12M1/36 , G01N33/50 , C12N5/071 , C12N5/00
CPC分类号： C12M41/46 , C12M23/16 , C12M23/44 , C12M41/48 , C12N5/0062 , C12N5/0671 , G01N33/5008 , G01N33/5067
摘要： A cell culture method using an in vitro microscale cell culture device having a first microscale chamber containing cells attached to an inner surface of the chamber and cell culture medium in contact with the cells. The cells are maintained in culture in the in vitro microscale cell culture device while flowing culture medium through the first chamber at a controlled rate to provide a liquid residence time value for the cells in the first chamber of the in vitro microscale cell culture device that is comparable to a liquid residence time value obtained with respect to comparable cells in vivo, such that metabolism by the cells in culture in the in vitro microscale cell culture device is comparable to metabolism by comparable cells in vivo.
摘要翻译：一种使用体外微细胞培养装置的细胞培养方法，所述培养装置具有包含连接于所述腔室内表面的细胞的第一微量室和与细胞接触的细胞培养基。将细胞在体外微量细胞培养装置中保持培养，同时以受控的速率流过培养基通过第一室，以提供体外微量细胞培养装置的第一室中的细胞的液体停留时间值，其为相当于在体内相当的细胞获得的液体停留时间值，使得体外微量细胞培养装置中培养物中的细胞的代谢与体内相当的细胞的代谢相当。

29. 发明申请

US20160030638A1 USE OF PERFUSION DECELLULARIZED LIVER FOR ISLET CELL RECELLULARIZATION 审中-公开
标题翻译：用于细胞增殖的脂肪细胞生长因子的使用
公开(公告)号：US20160030638A1
公开(公告)日：2016-02-04
申请号：US14777360
申请日：2014-03-13
申请人： MIROMATRIX MEDICAL INC.
发明人： Jeffrey Ross
IPC分类号： A61L27/38 , C12N5/071 , A61L27/40 , A61L27/36 , A61L27/58 , A61L27/54
CPC分类号： A61L27/3834 , A61L27/3633 , A61L27/3683 , A61L27/40 , A61L27/54 , A61L27/58 , C12N5/0671 , C12N5/0676 , C12N5/0678 , C12N5/069 , C12N5/0697 , C12N2501/115 , C12N2501/999 , C12N2502/28
摘要： The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
摘要翻译：本发明提供一种制备基于灌注的胰岛细胞的方法，所述胰岛细胞含有肝细胞外基质，胰岛细胞细胞外细胞外基质，以及使用细胞外基质的方法。

30. 发明申请

US20140274802A1 ENGINEERED LIVER TISSUES, ARRAYS THEREOF, AND METHODS OF MAKING THE SAME 有权
标题翻译：工程化肝脏组织，其阵列及其制备方法
公开(公告)号：US20140274802A1
公开(公告)日：2014-09-18
申请号：US13841430
申请日：2013-03-15
申请人： ORGANOVO, INC.
发明人： Benjamin R. Shepherd , Justin B. Robbins , Vivian A. Gorgen , Sharon C. Presnell
IPC分类号： C12N5/071 , G01N33/50
CPC分类号： C12N5/0697 , C12N5/0656 , C12N5/0671 , C12N2502/14 , C12N2502/28 , C12N2533/30 , C12N2535/10 , G01N33/5008 , G01N33/5088
摘要： Engineered, living, three-dimensional liver tissue constructs comprising: one or more layers, wherein each layer contains one or more liver cell types, the one or more layers cohered to form a living, three-dimensional liver tissue construct. In some embodiments, the constructs are characterized by having at least one of: at least one layer comprising a plurality of cell types, the cell types spatially arranged relative to each other to create a planar geometry; and a plurality of layers, at least one layer compositionally or architecturally distinct from at least one other layer to create a laminar geometry. Also disclosed are arrays and methods of making the same. Also disclosed are engineered, living, three-dimensional liver tissue constructs for use in the augmentation or restoration of one or more liver functions, by in vivo delivery of tissue or utilization of tissue in an extracorporeal device.
摘要翻译：包括：一层或多层，其中每层含有一种或多种肝细胞类型的工程化，活的三维肝组织构建体，所述一层或多层粘合形成活的三维肝组织构建体。在一些实施方案中，构建体的特征在于具有以下至少一个：包含多个细胞类型的至少一个层，所述细胞类型相对于彼此空间布置以产生平面几何形状; 以及多个层，至少一个层与至少一个其它层组成或结构上不同以产生层状几何形状。还公开了制造它们的阵列和方法。还公开了用于增加或恢复一种或多种肝功能的工程化的，活的三维肝组织构建体，通过体外递送组织或在体外装置中利用组织。

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